EKS-MUM-00323

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00323

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/BUB	309.2	5.0E-92	742	1007	266

Status

Reviewed

Ensembl Protein

ENSMUSP00000037126

UniProt Accession

Q9Z1S0; A2ARS1;

Protein Name

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Protein Synonyms/Alias

MAD3/BUB1-related protein kinase; BubR1; Mitotic checkpoint kinase MAD3L;

Gene Name

Bub1b

Gene Synonyms/Alias

Bub1b; Mad3l;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000040084	ENSMUSP00000037126	ENSMUST00000038341

Organism

Mus musculus

Functional Description

Essential component of the mitotic checkpoint. Requiredfor normal mitosis progression and tumor suppression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. Essential for tumor suppression. May play a role in regulating aging and fertility (By similarity).

Protein Length

1052

Protein Sequence
(FASTA)

MAEASEAMCL EGAEWELSKE NIQPLRHGRV MSTLQGALAK QESAGHTALQ QQKRAFESEI 60

RFYSGDDPLD VWDRYINWTE QNYPQGGKES NMSALVERAI EALQGETRYY NDPRFLSLWI 120

KLGHLCNEPL DMYSYLQSQG IGVSLAQFYI SWAEEYEARE NFKKADIIFQ EGIERKAEPL 180

DRLQSQHRQF QSRVSRQAFL ALGNEEEEAL EPSEPQRSSL AELKSRGKKM ARAPISRVGG 240

ALKAPGQSRG FLNAVPQPVH GNRRITVFDE NADTASRTEL SKPVAQPWMA PPVPRAKENE 300

LQPGPWSTDR PAGRRPHDNP ASVTSIPSVL PSFTPYVEES AQQTVMTPCK IEPSINHVLS 360

TRKPGREEGD PLQRVQSHQQ GCEEKKEKMM YCKEKIYAGV GEFSFEEIRA EVFRKKLKER 420

REAELLTSAK KREEMQKQIE EMERRLKAMQ AVQQEGAGGQ QEEKMPTEDP ARLQIASGPQ 480

EMSGVPLSCS ICPLSSNPRE ISPAENILQE QPDSKGSSMP FSIFDESLSD KKDKSPATGG 540

PQVLNAQRRP LSVLKTTEVG TTNEDVSPDI CDELTELEPL SEDAIITGFR NVTLCPNPED 600

TCDFARAARL ASTPFHEILS SKGIAADPEG LLQEEDLDGK AAEAHHTVHH QALIIKKLSP 660

IIEESREATH SSGFSRSSSS APSTSSIKGF QLLEKLELTN DGAEDAIQSP WCSQYRLQLL 720

KSLLELSAFA EFSVEDRPMP VLEIGKEIEL GPEDYVIKQE HLTCDDYRLF WVAPRSSAEL 780

TMIKASSQPI PWDFYINLKL KERLNEDYDQ LCSCCQYQDG HVVWYQYINC STLQNLLQHS 840

EFVTHEIIVL IIYNLLTIVE KLHRAEIVHG DLSPRSLILR NRIHDPYDYV NKDDHAVRIM 900

DFSYSVDLRV QLDAFAYSGF RTAQILEGQK ILANCSSPYH VDLLGIADLA HLLLFKEHLH 960

VFWDGLLWKL SQSTSELKDS ELWNKFFVRI LNASDKSTVS VLGELAAEMG GAFDATFHSH 1020

LNRALWKLGK TISPEALLTQ QDKQPGGSQS PA 1052

Nucleotide Sequence
(FASTA)

ATGGCGGAGG CGAGTGAAGC CATGTGCCTG GAGGGAGCAG AGTGGGAGCT GAGTAAAGAA 60

AACATACAGC CCTTACGGCA CGGGCGGGTC ATGTCCACAC TTCAGGGAGC TTTGGCAAAG 120

CAAGAGTCAG CCGGCCACAC TGCTCTGCAG CAGCAGAAAC GGGCATTTGA ATCTGAAATC 180

CGCTTTTACT CTGGAGATGA CCCTCTGGAT GTGTGGGACA GATATATTAA TTGGACAGAA 240

CAGAACTACC CTCAAGGGGG GAAAGAGAGT AACATGTCAG CGTTAGTGGA GAGAGCGATA 300

GAAGCACTCC AAGGAGAGAC GCGCTATTAT AATGACCCCC GCTTTCTCAG TCTCTGGATC 360

AAATTGGGAC ATTTGTGCAA TGAACCTTTG GATATGTACA GCTATTTACA AAGCCAAGGA 420

ATTGGCGTTT CCCTTGCCCA GTTCTATATT TCATGGGCTG AAGAATACGA AGCTAGAGAA 480

AATTTCAAGA AAGCGGACAT AATATTCCAG GAAGGGATTG AACGCAAGGC TGAGCCCCTG 540

GACAGACTGC AGTCCCAGCA CAGACAGTTC CAGTCTCGAG TGTCGCGACA AGCTTTCTTG 600

GCCCTTGGGA ATGAAGAGGA GGAGGCTTTG GAGCCTTCTG AACCACAGAG AAGCTCGCTA 660

GCTGAGCTGA AGAGCAGAGG GAAGAAGATG GCCAGAGCGC CCATCAGCCG TGTCGGAGGT 720

GCTCTGAAAG CTCCAGGTCA GAGCAGAGGG TTCCTAAATG CAGTTCCCCA GCCAGTACAC 780

GGTAATCGCA GGATCACCGT TTTTGATGAA AATGCCGATA CCGCGTCTAG AACGGAGTTA 840

TCTAAGCCTG TAGCCCAGCC ATGGATGGCA CCCCCTGTGC CCAGGGCCAA AGAGAACGAA 900

CTTCAGCCAG GCCCATGGAG CACAGACAGG CCGGCGGGAC GCAGGCCTCA TGACAATCCA 960

GCCTCTGTGA CGTCGATTCC CAGCGTGCTT CCCAGCTTTA CGCCGTACGT GGAAGAGAGC 1020

GCCCAGCAGA CAGTCATGAC ACCATGCAAG ATTGAGCCTA GTATCAACCA TGTTCTCAGC 1080

ACCAGGAAGC CAGGGAGAGA AGAAGGAGAC CCCTTGCAGA GAGTTCAGAG TCATCAGCAA 1140

GGCTGTGAGG AGAAGAAGGA GAAGATGATG TACTGTAAGG AGAAGATCTA TGCCGGAGTT 1200

GGGGAGTTCT CCTTTGAGGA GATCCGAGCT GAAGTGTTCC GAAAGAAGCT GAAAGAACGA 1260

AGGGAAGCCG AGCTGTTGAC CAGTGCAAAG AAGAGGGAGG AGATGCAGAA GCAGATCGAA 1320

GAGATGGAGA GGAGGCTGAA GGCAATGCAG GCTGTTCAGC AAGAAGGAGC TGGGGGCCAG 1380

CAAGAAGAGA AGATGCCTAC AGAGGACCCA GCCAGATTGC AGATTGCTTC GGGGCCTCAG 1440

GAAATGTCGG GAGTTCCTCT GTCCTGTTCC ATCTGTCCAC TAAGCTCGAA TCCTAGGGAA 1500

ATTTCACCTG CTGAGAACAT TTTGCAAGAA CAGCCTGATT CTAAAGGTTC CAGTATGCCT 1560

TTCTCCATTT TTGATGAGTC TCTTTCAGAC AAAAAGGACA AAAGTCCTGC TACAGGTGGT 1620

CCACAGGTTC TCAATGCCCA GAGAAGACCC CTTTCAGTTC TCAAAACTAC AGAAGTGGGC 1680

ACCACAAATG AGGATGTGTC TCCCGATATT TGTGATGAAC TCACAGAACT TGAGCCTCTG 1740

AGTGAAGACG CCATCATCAC TGGTTTCAGG AACGTCACTC TCTGTCCCAA CCCTGAGGAC 1800

ACTTGTGACT TTGCTAGAGC AGCTCGTTTG GCATCTACTC CTTTCCATGA GATACTGTCC 1860

TCGAAGGGCA TCGCTGCTGA TCCCGAGGGA CTGTTGCAGG AAGAGGATCT GGATGGGAAG 1920

GCCGCCGAGG CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA 1980

ATTATTGAAG AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA 2040

GCTCCCAGTA CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT 2100

GACGGGGCAG AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA 2160

AAATCCCTAC TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT 2220

GTGCTGGAAA TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG 2280

CACCTAACAT GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA 2340

ACCATGATAA AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG 2400

AAGGAGCGTC TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC 2460

CATGTTGTTT GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC 2520

GAATTTGTTA CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG 2580

AAGCTACACA GAGCTGAAAT AGTACACGGA GACTTGAGTC CACGGAGTCT GATCCTACGA 2640

AACAGAATCC ACGACCCCTA TGACTATGTA AATAAGGACG ATCACGCTGT GAGGATCATG 2700

GACTTCTCCT ACAGTGTTGA CCTGAGGGTG CAGCTGGATG CGTTTGCCTA TAGTGGCTTT 2760

CGGACTGCAC AGATCCTGGA AGGACAAAAG ATCCTGGCTA ACTGTTCTTC TCCCTACCAT 2820

GTAGATCTGT TGGGTATAGC AGACCTAGCG CACTTACTCC TGTTCAAGGA GCACCTCCAT 2880

GTCTTCTGGG ATGGACTCCT CTGGAAACTT AGCCAGAGCA CCTCTGAGCT AAAAGACAGT 2940

GAATTGTGGA ATAAATTCTT TGTGCGGATT CTGAATGCCA GTGACAAGTC CACAGTGTCT 3000

GTTCTGGGGG AGCTGGCAGC AGAAATGGGT GGGGCTTTTG ATGCCACATT CCATAGCCAC 3060

CTGAACAGAG CCCTGTGGAA GCTGGGGAAG ACAATCAGCC CGGAAGCTTT GCTCACTCAG 3120

CAAGACAAGC AGCCAGGCGG CTCCCAGAGC CCTGCCTAA 3159

Domain Profile

S: 1     leiekeigeGayakvykaeh....dsk........sgelvalKvekpansWefYIclqlk  48

         lei kei+ G++++v+k+eh    d++        s+el+++K+++++++W+fYI+l+lk

Q: 742   LEIGKEIELGPEDYVIKQEHltcdDYRlfwvaprsSAELTMIKASSQPIPWDFYINLKLK  801

         689*****************999877788889998*************************

S: 49    eRlkeeellksiieissallfqdasllvleyasygtlldlinkvkevtnkvmdeelvvll  108

         eRl+e+     +++++s++++qd+++++++y++++tl++l+++++ vt     +e++vl+

Q: 802   ERLNED-----YDQLCSCCQYQDGHVVWYQYINCSTLQNLLQHSEFVT-----HEIIVLI  851

         ****99.....*************************************.....*******

S: 109   ivqllkiveklHeveIiHgDlkPdnfllgnrimdkad.kdkeekglkliDfgrsiDmkll  167

         i++ll+iveklH++eI+HgDl+P++++l+nri+d++d ++k++++++++Df++s+D++++

Q: 852   IYNLLTIVEKLHRAEIVHGDLSPRSLILRNRIHDPYDyVNKDDHAVRIMDFSYSVDLRVQ  911

         *************************************99*********************

S: 168   pdgtkfkgkaktdafeciemlagkswsYqvDlfglaavihvllfgkyleviqeggswklk  227

         +d+++++g+++++++e++++la++s++Y+vDl+g+a+++h+llf+++l+v+++g  wkl+

Q: 912   LDAFAYSGFRTAQILEGQKILANCSSPYHVDLLGIADLAHLLLFKEHLHVFWDGLLWKLS  971

         ************************************************************

S: 228   qkikrllkkelwekiferlLNisdastlpsleelve  263

         q++++l+++elw+k+f+r+LN+sd+st+++l+el++

Q: 972   QSTSELKDSELWNKFFVRILNASDKSTVSVLGELAA  1007

         *********************************986

Domain Sequence
(FASTA)

LEIGKEIELG PEDYVIKQEH LTCDDYRLFW VAPRSSAELT MIKASSQPIP WDFYINLKLK 60

ERLNEDYDQL CSCCQYQDGH VVWYQYINCS TLQNLLQHSE FVTHEIIVLI IYNLLTIVEK 120

LHRAEIVHGD LSPRSLILRN RIHDPYDYVN KDDHAVRIMD FSYSVDLRVQ LDAFAYSGFR 180

TAQILEGQKI LANCSSPYHV DLLGIADLAH LLLFKEHLHV FWDGLLWKLS QSTSELKDSE 240

LWNKFFVRIL NASDKSTVSV LGELAA 266

Keyword

Acetylation; Apoptosis; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Kinase; Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0000940	; C:condensed chromosome outer kinetochore
GO:0000778	; C:condensed nuclear chromosome kinetochore
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0051301	; P:cell division
GO:0007091	; P:mitotic metaphase/anaphase transition
GO:0071459	; P:protein localization to chromosome, centromeric region
GO:0034501	; P:protein localization to kinetochore

KEGG

mmu:12236

;

InterPros

IPR011009	; Kinase-like_dom.
IPR013212	; Mad3_BUB1_I.
IPR000719	; Prot_kinase_cat_dom.
IPR015661	; Ser/Thr_kinase_Bub1.

Pfam

PF08311

; Mad3_BUB1_I; 1.

SMARTs

SM00777

; Mad3_BUB1_I; 1.

Prosites

PS51489	; BUB1_N; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013