EKS-MUM-00164

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00164

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/TLK	615.7	2.2E-185	456	734	279

Status

Reviewed

Ensembl Protein

ENSMUSP00000035961

UniProt Accession

Q8C0V0;

Protein Name

Serine/threonine-protein kinase tousled-like 1

Protein Synonyms/Alias

Tousled-like kinase 1;

Gene Name

Tlk1

Gene Synonyms/Alias

Tlk1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000041997	ENSMUSP00000035961	ENSMUST00000038584

Organism

Mus musculus

Functional Description

Rapidly and transiently inhibited by phosphorylationfollowing the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly (By similarity). Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10' (By similarity).

Protein Length

766

Protein Sequence
(FASTA)

MSVQSSSGSL EGPPSWSRLS TSPTPGSAAA ARSLLNHTPP SGRPREGAMD ELHSLDPRRQ 60

ELLEARFTGV ATGSTGSTGS CSVGAKASTN NESSNHSFGS LGSLSDKESE TPEKKQSESS 120

RGRKRKAESQ NESSQGKSIG GRGHKISDYF EYQGGNGSSP VRGIPPAIRS PQNSHSHSTP 180

SSSVRPNSPS PTALAFGDHP VVQPKQLSFK ITQTDLTMLK LAALESTKNQ DLEKKEGRID 240

DLLRANCDLR RQIDDQQKLL EKYKERLNKC ISMSKKLLIE KSTQEKLSSR EKSMQDRLRL 300

GHFTTVRHGA SFTEQWTDGF AFQNLVKQQE WVNQQREDIE RQRKLLGKRK PPTANNSQAP 360

ATNSEAKQRK TKAVNGAEND PFVRPNLPQL LTLAEYHEQE EIFKLRLGHL KKEEAEIQAE 420

LERLERVRNL HIRELKRINN EDNSQFKDHP TLNERYLLLH LLGRGGFSEV YKAFDLYEQR 480

YAAVKIHQLN KSWRDEKKEN YHKHACREYR IHKELDHPRI VKLYDYFSLD TDTFCTVLEY 540

CEGNDLDFYL KQHKLMSEKE ARSIVMQIVN ALRYLNEIKP PIIHYDLKPG NILLVDGTAC 600

GEIKITDFGL SKIMDDDSYG VDGMDLTSQG AGTYWYLPPE CFVVGKEPPK ISNKVDVWSV 660

GVIFFQCLYG RKPFGHNQSQ QDILQENTIL KATEVQFPVK PVVSSEAKAF IRRCLAYRKE 720

DRFDVHQLAN DPYLLPHMRR SNSSGNLHMS GLTATPTPPS SSIITY 766

Nucleotide Sequence
(FASTA)

ATGAGTGTCC AAAGTAGCAG TGGAAGTTTG GAGGGGCCGC CATCTTGGTC CCGGCTCTCC 60

ACGTCTCCAA CCCCGGGCTC GGCGGCGGCG GCCAGGTCCC TGCTGAATCA CACGCCGCCA 120

TCCGGGAGGC CCAGGGAAGG TGCAATGGAT GAGCTTCACA GTCTGGACCC AAGAAGGCAA 180

GAATTATTGG AAGCAAGATT TACTGGAGTT GCTACTGGGA GTACTGGGAG TACTGGGAGT 240

TGCAGTGTTG GAGCAAAAGC TTCAACAAAT AATGAAAGTT CTAATCACAG TTTTGGAAGC 300

TTAGGATCTC TAAGTGACAA AGAATCAGAG ACACCAGAGA AGAAACAATC AGAGTCATCC 360

AGGGGAAGAA AGAGAAAAGC AGAAAGTCAG AATGAAAGTA GTCAGGGAAA AAGTATCGGG 420

GGACGTGGCC ATAAGATCAG CGACTATTTT GAATACCAAG GTGGAAATGG CTCGAGTCCA 480

GTAAGAGGCA TCCCTCCAGC AATCCGTTCT CCGCAGAACT CACACTCACA TTCCACTCCT 540

TCCTCATCTG TTCGACCGAA TAGCCCTTCT CCTACTGCAT TAGCTTTCGG GGACCACCCT 600

GTTGTACAAC CAAAGCAGTT ATCCTTTAAG ATCACTCAAA CTGATCTTAC AATGCTGAAA 660

TTGGCAGCAT TAGAAAGTAC TAAAAATCAA GACCTGGAGA AGAAGGAAGG CCGGATTGAT 720

GATCTGCTCA GGGCCAACTG TGATCTCAGG CGGCAAATAG ATGATCAACA GAAATTACTT 780

GAAAAATACA AAGAACGATT AAATAAGTGC ATATCTATGA GCAAGAAACT ACTTATTGAA 840

AAGAGTACAC AAGAAAAATT GTCTAGCAGA GAAAAGAGTA TGCAAGATCG GTTGCGCCTT 900

GGGCACTTTA CCACAGTTAG ACACGGCGCT TCCTTTACAG AACAGTGGAC AGATGGTTTT 960

GCATTTCAGA ATCTTGTGAA GCAACAAGAA TGGGTGAATC AACAAAGGGA AGACATTGAA 1020

CGGCAAAGAA AACTTCTAGG CAAACGCAAA CCTCCCACAG CTAATAATTC TCAGGCACCT 1080

GCTACCAATT CTGAAGCCAA GCAAAGAAAG ACCAAGGCAG TCAATGGAGC GGAAAACGAT 1140

CCCTTTGTTA GGCCAAATTT ACCACAACTG CTGACTCTGG CAGAGTATCA TGAACAAGAG 1200

GAAATTTTTA AACTTAGACT AGGACATCTC AAAAAGGAAG AGGCTGAAAT CCAGGCGGAA 1260

CTGGAGCGTT TGGAGAGGGT CCGGAACCTT CACATCCGAG AGCTGAAGAG AATAAACAAT 1320

GAGGATAATT CACAGTTCAA AGATCATCCA ACGTTAAATG AAAGATATTT ATTACTTCAT 1380

CTGCTTGGGA GAGGCGGCTT TAGTGAGGTC TATAAGGCAT TCGACTTGTA TGAGCAAAGA 1440

TACGCTGCTG TGAAGATCCA CCAGCTTAAT AAGAGCTGGA GAGACGAGAA GAAAGAAAAC 1500

TACCACAAAC ACGCTTGCCG AGAGTATCGA ATACACAAGG AACTGGACCA CCCCAGAATA 1560

GTTAAACTCT ATGATTACTT CTCCTTGGAT ACAGATACGT TTTGTACAGT GCTAGAGTAC 1620

TGTGAAGGCA ATGACTTGGA CTTCTACCTG AAGCAGCACA AGTTGATGTC TGAGAAAGAA 1680

GCTCGGTCTA TTGTGATGCA GATTGTGAAT GCCTTACGCT ATCTCAATGA AATCAAACCT 1740

CCTATTATAC ATTATGACCT TAAGCCAGGA AATATCCTTC TGGTAGATGG AACAGCATGT 1800

GGAGAAATCA AAATCACTGA TTTTGGTCTG TCCAAGATTA TGGATGACGA TAGTTACGGT 1860

GTTGATGGGA TGGATCTGAC TTCCCAGGGG GCAGGCACTT ACTGGTATTT ACCTCCTGAG 1920

TGCTTTGTAG TTGGAAAAGA GCCACCGAAG ATCTCCAACA AGGTTGACGT GTGGTCAGTT 1980

GGGGTCATCT TCTTCCAGTG CCTCTATGGT AGAAAACCAT TTGGTCACAA TCAATCTCAA 2040

CAAGACATTC TACAGGAAAA CACAATACTG AAAGCCACAG AAGTCCAGTT CCCTGTAAAA 2100

CCTGTTGTAA GCAGTGAAGC CAAGGCCTTC ATAAGACGCT GTTTGGCTTA CCGAAAAGAA 2160

GATCGATTTG ATGTGCACCA GCTGGCAAAT GACCCATACC TTCTGCCACA CATGAGAAGA 2220

TCAAATTCTT CAGGAAACTT ACATATGTCT GGGCTGACAG CAACTCCCAC ACCTCCTTCT 2280

TCAAGCATAA TTACTTACTG A 2301

Domain Profile

S: 1     ylllkllgkggfsevykafdlkeqryvavkihqlnkdwkdekkenyvkhalreyrihkel  60

         ylll+llg+ggfsevykafdl+eqry+avkihqlnk+w+dekkeny+kha+reyrihkel

Q: 456   YLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKEL  515

         9***********************************************************

S: 61    dhprivklydllsldtdsfctvleyvegndldfylkqhkllsekearsiimqivsalkyl  120

         dhprivklyd++sldtd+fctvley+egndldfylkqhkl+sekearsi+mqiv+al+yl

Q: 516   DHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYL  575

         ************************************************************

S: 121   neikppiihydlkpgnillvegtacgeikitdfglskimdddsydsddgleltsqgagty  180

         neikppiihydlkpgnillv+gtacgeikitdfglskimdddsy++d g++ltsqgagty

Q: 576   NEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVD-GMDLTSQGAGTY  634

         *********************************************99.************

S: 181   wylppecfvvgkeppkisnkvdvwsvgvifyqclygkkpfghnqsqqdileentilkate  240

         wylppecfvvgkeppkisnkvdvwsvgvif+qclyg+kpfghnqsqqdil+entilkate

Q: 635   WYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATE  694

         ************************************************************

S: 241   vqfpskpvvsseakafirrclayrkedridvqqlakdeyl  280

         vqfp+kpvvsseakafirrclayrkedr+dv+qla+d+yl

Q: 695   VQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYL  734

         **************************************97

Domain Sequence
(FASTA)

YLLLHLLGRG GFSEVYKAFD LYEQRYAAVK IHQLNKSWRD EKKENYHKHA CREYRIHKEL 60

DHPRIVKLYD YFSLDTDTFC TVLEYCEGND LDFYLKQHKL MSEKEARSIV MQIVNALRYL 120

NEIKPPIIHY DLKPGNILLV DGTACGEIKI TDFGLSKIMD DDSYGVDGMD LTSQGAGTYW 180

YLPPECFVVG KEPPKISNKV DVWSVGVIFF QCLYGRKPFG HNQSQQDILQ ENTILKATEV 240

QFPVKPVVSS EAKAFIRRCL AYRKEDRFDV HQLANDPYL 279

Keyword

ATP-binding; Cell cycle; Chromatin regulator; Coiled coil; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0016568	; P:chromatin modification
GO:0006886	; P:intracellular protein transport
GO:0035556	; P:intracellular signal transduction
GO:0001672	; P:regulation of chromatin assembly or disassembly
GO:0006974	; P:response to DNA damage stimulus

KEGG

mmu:228012

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013