EKS-MUM-00409

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00409

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	346.8	1.8E-103	138	396	259

Status

Reviewed

Ensembl Protein

ENSMUSP00000041819

UniProt Accession

Q3U1V8; E9QLZ4; Q8BIG8;

Protein Name

Mitogen-activated protein kinase kinase kinase 9

Protein Synonyms/Alias

Gene Name

Map3k9

Gene Synonyms/Alias

Map3k9;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000042724	ENSMUSP00000041819	ENSMUST00000035987

Organism

Mus musculus

Functional Description

Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis (By similarity).

Protein Length

1077

Protein Sequence
(FASTA)

MESSRSLLGC LASATAAPPG DDATGAGAEE EEDEEEAAAE LGSHAALPYW TAVFEYEAAG 60

EDELTLRLGD VVEVLSKDSQ VSGDEGWWTG QLNQRVGIFP SNYVTPRSAF SSRCQPGAED 120

PSCYPPIQLL EIDFAELTLE EIIGIGGFGK VYRAFWAGDE VAVKAARHDP DEDISQTIEN 180

VRQEAKLFAM LKHPNIIALR GVCLKEPNLC LVMEFARGGP LNRVLSGKRI PPDILVNWAV 240

QIARGMNYLH DEAIVPIIHR DLKSSNILIL QKVENGDLSN KILKITDFGL AREWHRTTKM 300

SAAGTYAWMA PEVIRASMFS KGSDVWSYGV LLWELLTGEV PFRGIDGLAV AYGVAMNKLA 360

LPIPSTCPEP FAKLMEDCWN PDPHSRPSFT SILDQLTTIE ESGFFEMPKD SFHCLQDDWK 420

HEIQEMFDQL RAKEKELRTW EEELTRAALQ QKNQEELLRR REQELAEREI DILERELNII 480

IHQLCQEKPR VKKRKGKFRK SRLKLKDGNR ISLPSDFQHK FTVQASPTMD KRKSLISNRS 540

SPPASPTIIP RLRAIQLTPG ESSKTWGRSS VVPKEEGEEE EKRAPKKKGR TWGPGTLGQK 600

ELTSGDEGLK SLVDGYKQWS SSAPNLGKGP RSSPALPGFT SLMEIEDEDS EGPGSGENHQ 660

QHSPNQSYLC IPFPRGEDGD GPSSDGVHEE PTPVNSATST PQLTPTNSLK RGGTHHRRCE 720

VALLGCGAVL AATGLGFDLL EAGKCQLLPP EEPEPPAREE KKRREGLFQR ASRPRRSTSP 780

PSRKLFKKEE PMTLLGDPSA SLTLLSLSSI SECNSTRSLL RSDSDEIVVY EMPVSPVEAP 840

PLTQCTHNPL VNVRVERFKR DPNQSLTPTH VTLTAPTQPS GHRRTPSDGA LKPTAAPAVL 900

GSRSPSSNGM SPSPGTGMLK TPSPSRDPGE FPRLPDPNVV FPPTPRRWNT QRDSTLERPK 960

TLEFLPRPRP SANRQRLDPW WFVSPSHARS ASPANSSSTE TPSNLDSCFA SSSSTVEERP 1020

GLPALLPLQA GPLLPAERTL LDLDAEGQSQ DSTVPLCRAE LNAHGPSPYE IQQEFWS 1077

Nucleotide Sequence
(FASTA)

ATGGAGTCCT CCAGATCGCT TCTCGGCTGC CTGGCGAGTG CCACTGCCGC CCCGCCGGGG 60

GACGATGCCA CGGGCGCTGG GGCCGAAGAG GAGGAGGACG AGGAGGAGGC GGCGGCCGAG 120

CTGGGATCTC ACGCCGCGCT GCCCTACTGG ACGGCTGTGT TCGAGTACGA GGCGGCGGGC 180

GAGGACGAGC TGACCCTGCG GCTGGGCGAT GTGGTAGAGG TGCTGTCCAA GGACTCGCAG 240

GTGTCCGGCG ATGAGGGCTG GTGGACCGGA CAGCTGAACC AGCGGGTGGG CATCTTCCCC 300

AGCAACTACG TGACCCCGCG TAGCGCCTTC TCCAGCCGCT GCCAGCCGGG CGCCGAGGAC 360

CCCAGCTGCT ACCCGCCCAT TCAGCTGTTA GAGATTGATT TTGCGGAGCT AACCCTGGAG 420

GAGATCATCG GCATTGGGGG CTTTGGGAAA GTTTATCGTG CTTTCTGGGC GGGCGATGAG 480

GTGGCCGTGA AGGCAGCTCG TCACGACCCT GATGAGGACA TCAGCCAGAC CATAGAGAAC 540

GTTCGCCAAG AGGCCAAGCT CTTTGCCATG CTGAAGCACC CGAACATCAT TGCGCTCAGA 600

GGGGTGTGCC TGAAGGAACC CAACCTCTGC TTGGTCATGG AGTTTGCTCG TGGAGGGCCT 660

CTGAACAGAG TATTGTCTGG GAAGAGGATT CCCCCGGACA TCCTGGTGAA CTGGGCCGTG 720

CAGATCGCCA GAGGGATGAA CTATCTACAT GATGAGGCGA TCGTACCCAT CATCCACCGA 780

GACCTTAAGT CCAGCAACAT ATTGATCCTG CAGAAAGTGG AGAATGGAGA CCTGAGTAAC 840

AAGATTCTGA AGATCACCGA CTTTGGGCTG GCGCGGGAAT GGCACCGGAC CACCAAGATG 900

AGTGCGGCGG GAACATACGC TTGGATGGCA CCTGAAGTCA TCCGTGCTTC CATGTTTTCC 960

AAAGGCAGCG ATGTGTGGAG CTACGGTGTA CTGCTTTGGG AGCTGTTGAC TGGCGAGGTG 1020

CCCTTCCGGG GCATTGATGG CTTAGCAGTG GCTTACGGTG TGGCCATGAA CAAACTCGCC 1080

CTTCCTATCC CCTCTACATG TCCAGAGCCT TTTGCCAAAC TCATGGAAGA CTGCTGGAAT 1140

CCCGACCCCC ACTCGCGCCC ATCTTTCACG AGTATCCTGG ACCAGCTAAC GACTATAGAG 1200

GAGTCCGGTT TCTTTGAGAT GCCCAAGGAC TCCTTCCACT GCCTGCAGGA CGACTGGAAA 1260

CATGAGATTC AGGAGATGTT TGACCAACTC AGGGCCAAAG AAAAGGAGCT CCGAACCTGG 1320

GAAGAGGAGC TGACCCGGGC TGCGCTCCAG CAGAAGAACC AGGAGGAGCT GCTCCGGCGT 1380

AGGGAGCAGG AGCTGGCGGA GCGGGAGATC GACATCCTGG AGCGAGAGCT CAATATCATC 1440

ATCCACCAGC TGTGCCAGGA AAAGCCCCGG GTGAAGAAAC GCAAGGGCAA GTTCAGGAAG 1500

AGCCGGCTGA AGCTCAAGGA CGGCAACCGC ATCAGCCTCC CCTCCGATTT CCAGCACAAG 1560

TTCACGGTGC AGGCCTCCCC GACCATGGAT AAAAGGAAGA GTCTGATCAG CAACCGGTCG 1620

AGTCCTCCTG CAAGCCCCAC CATCATCCCT CGCCTTCGAG CCATCCAGTT GACACCTGGT 1680

GAAAGCAGTA AAACCTGGGG CCGGAGCTCA GTTGTTCCAA AAGAGGAAGG GGAGGAGGAG 1740

GAGAAGAGGG CCCCAAAGAA GAAGGGCCGG ACATGGGGAC CAGGAACACT TGGGCAGAAA 1800

GAGCTCACAT CAGGAGATGA AGGCCTCAAG TCCCTGGTAG ATGGATACAA GCAGTGGTCA 1860

TCCAGTGCCC CCAACCTGGG GAAGGGCCCA AGGAGTAGCC CCGCCCTACC TGGGTTCACC 1920

AGCCTTATGG AGATAGAGGA TGAGGATAGC GAAGGCCCAG GAAGCGGAGA GAATCATCAA 1980

CAGCATTCAC CCAACCAGTC CTACCTCTGT ATCCCGTTTC CTCGTGGAGA GGATGGGGAT 2040

GGCCCCTCTA GTGATGGAGT TCATGAGGAA CCTACCCCAG TCAACTCAGC TACCAGTACC 2100

CCTCAGCTGA CGCCAACCAA CAGCCTCAAG CGCGGTGGGA CCCACCACCG GCGCTGTGAG 2160

GTGGCTCTGC TCGGATGTGG AGCTGTTCTG GCAGCCACAG GCCTAGGGTT TGACTTGCTG 2220

GAAGCTGGCA AGTGCCAGCT GCTTCCCCCA GAGGAACCTG AGCCACCAGC CCGGGAGGAG 2280

AAGAAGAGGC GTGAAGGTCT TTTCCAAAGG GCCAGCCGCC CTCGTCGGAG TACCAGCCCC 2340

CCCTCCCGAA AGCTCTTCAA GAAGGAAGAG CCAATGACGT TGCTAGGAGA CCCCTCTGCC 2400

TCCTTGACAC TGCTGTCTCT CTCCTCCATC TCTGAATGCA ACTCTACCCG CTCCCTACTG 2460

CGCTCTGACA GTGATGAGAT CGTGGTGTAT GAAATGCCAG TCAGCCCAGT TGAAGCTCCA 2520

CCCCTAACCC AATGTACCCA CAACCCCCTG GTTAATGTCC GAGTGGAGCG CTTCAAGAGA 2580

GACCCCAACC AGTCCTTGAC TCCCACCCAT GTCACCCTCA CAGCCCCCAC GCAGCCTAGT 2640

GGTCACAGGC GGACTCCTTC TGATGGGGCT CTTAAGCCAA CAGCAGCCCC TGCAGTACTA 2700

GGCAGCAGGA GCCCCTCCAG CAATGGAATG AGTCCCAGTC CTGGAACAGG CATGTTGAAA 2760

ACTCCCAGTC CCAGCCGAGA CCCAGGTGAA TTTCCCCGTC TCCCTGACCC CAATGTGGTC 2820

TTTCCCCCAA CTCCAAGGCG CTGGAACACC CAGCGAGACT CTACCTTAGA GAGACCCAAG 2880

ACCCTGGAGT TTCTGCCTCG ACCGCGTCCT TCTGCCAACC GGCAGCGACT GGACCCTTGG 2940

TGGTTTGTGT CTCCCAGCCA TGCCCGCAGC GCCTCCCCAG CTAACAGCTC CAGCACAGAA 3000

ACACCCAGCA ACCTGGACTC CTGCTTTGCC AGCAGCAGCA GCACTGTGGA AGAGCGGCCT 3060

GGACTTCCAG CCCTGCTCCC TTTACAGGCA GGACCACTGC TCCCTGCTGA ACGGACACTT 3120

TTGGACCTGG ATGCTGAGGG GCAGAGCCAG GATAGCACTG TGCCCCTATG CAGAGCTGAG 3180

CTGAATGCAC ACGGGCCTTC CCCATATGAG ATCCAACAGG AGTTCTGGTC TTAG 3234

Domain Profile

S: 2     eleeligsgafgkvykakwrkkevavKklreeaee..kekleklereakllsklshpnii  59

         +lee+ig g fgkvy+a w ++evavK++r++  e  ++++e++++eakl+++l+hpnii

Q: 138   TLEEIIGIGGFGKVYRAFWAGDEVAVKAARHDPDEdiSQTIENVRQEAKLFAMLKHPNII  197

         5799****************************9998999*********************

S: 60    ellGvcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseap  119

         +l+Gvcl+epnl+lvme+++gg L++vl++   ++i+++ lv+wa+qiArGm+YLh+ea

Q: 198   ALRGVCLKEPNLCLVMEFARGGPLNRVLSG---KRIPPDILVNWAVQIARGMNYLHDEAI  254

         ******************************...99*************************

S: 120   kkiihrdLksknvLl.......seekktlkicdFGtarelskstkmsaagtlawmaPEvl  172

         ++iihrdLks+n+L+       + ++k lki+dFG are++++tkmsaagt+awmaPEv+

Q: 255   VPIIHRDLKSSNILIlqkvengDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVI  314

         ********************99999***********************************

S: 173   keekvseksDvwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkL  232

         +++ +s+ sDvwSygv+LWellt+evP+++++ la+ay+va+++l lpips+cpe+++kL

Q: 315   RASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKL  374

         ************************************************************

S: 233   mkecwnedpekRpsfseivksl  254

         m++cwn dp++Rpsf++i+++l

Q: 375   MEDCWNPDPHSRPSFTSILDQL  396

         ******************9876

Domain Sequence
(FASTA)

TLEEIIGIGG FGKVYRAFWA GDEVAVKAAR HDPDEDISQT IENVRQEAKL FAMLKHPNII 60

ALRGVCLKEP NLCLVMEFAR GGPLNRVLSG KRIPPDILVN WAVQIARGMN YLHDEAIVPI 120

IHRDLKSSNI LILQKVENGD LSNKILKITD FGLAREWHRT TKMSAAGTYA WMAPEVIRAS 180

MFSKGSDVWS YGVLLWELLT GEVPFRGIDG LAVAYGVAMN KLALPIPSTC PEPFAKLMED 240

CWNPDPHSRP SFTSILDQL 259

Keyword

Apoptosis; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Stress response; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0000186	; P:activation of MAPKK activity
GO:0006915	; P:apoptotic process
GO:0000165	; P:MAPK cascade
GO:0043065	; P:positive regulation of apoptotic process
GO:0046777	; P:protein autophosphorylation
GO:0006355	; P:regulation of transcription, DNA-dependent
GO:0006950	; P:response to stress
GO:0006351	; P:transcription, DNA-dependent

KEGG

mmu:338372

;

InterPros

IPR011009	; Kinase-like_dom.
IPR016231	; MAPKKK9/10/11.
IPR015785	; MAPKKK9/10/11-like.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR011511	; SH3_2.
IPR001452	; SH3_domain.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF07653	; SH3_2; 1.

SMARTs

SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50002	; SH3; 1.

Prints

PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013