EPS-MUM-00025

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00025

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	205.7	2.5E-60	1131	1376	246

Status

Reviewed

Ensembl Protein

ENSMUSP00000056530

UniProt Accession

Q8CHE4; Q6PCN0; Q8QZU8; Q8R5E5; Q99KL6;

Protein Name

PH domain leucine-rich repeat-containing protein phosphatase 1

Protein Synonyms/Alias

Pleckstrin homology domain-containing family E member 1; PH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein;

Gene Name

Phlpp1

Gene Synonyms/Alias

Phlpp1; Kiaa0606, Phlpp, Plekhe1, Scop;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000044340	ENSMUSP00000056530	ENSMUST00000061047

Organism

Mus musculus

Functional Description

Protein phosphatase that mediates dephosphorylation of'Ser-473' of AKT1, 'Ser-660' of PRKCB, and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity).

Protein Length

1687

Protein Sequence
(FASTA)

MEPAAAAPAQ RLADPTGEDQ ALAAAAAEGG RCPDPALSAA APSGGNGGAA REEAPCEAPP 60

GPLPGRAGGT GRRRRRGAPQ PAAGGAAPVP AAGGGANSLL LKRGRLKRNL SAAAAASSSS 120

SPSSASSAAG GLPASCSASA SLCTRSLDRK TLLLKHRQLL QLQPSDRDWV RHQLQRGCVH 180

VFDRHMASSY LRPVLCTLDT TAAEVAARLL QLGHKGGGVV KVLGYGPPPA AAPAASDQTL 240

DGEHGRDVEP PPSSGTVGAV RGPARAPPAD LPLPGGAWTR CAPRISPAPS DSSPGELFAG 300

GPGSPPRAPR PASDTESFSL SPSAESVSDR LDPYSSGGGG SSSSSEELEA DPAMPHRPGR 360

PAQPRPPSPK TSALLQPKAP TGVDSTGVIA GEGPGDDKAM AAAAPDVPLS TSGRIRETVQ 420

KTSPPSLYVQ LHGETTRRLE ADEKPLQIQN DYLFQLGFGE LWRVQEEGMD SEIGCLIRFY 480

AGKPHSTGSS ERIQLSGMYN VRKGKMQLPV NRWTRRQVIL CGTCLIVSSV KDSVSGKMHV 540

LPLIGGKVEE VKKHQHCLAF SSSGPQSQTY YICFDTFTEY LRWLRQVSKV ASQRISSVDL 600

SCCSLEHLPA NLFYSQDLTH LNLKQNFLRQ TPTLPAARGL GELQRFTKLK SLNLSNNHLG 660

AFPSAVCSIP TLAELNVSCN ALREVPAAVG DMQNLQTFLL DGNFLQSLPA ELESMHQLSY 720

LGLSFNEFTD IPEVLEKLTA VDKLCMAGNC VETLRLQALR RMPHIKHVDL RLNILRKLMA 780

DEVDFVQHVT QLDLRDNKLG DLDAMIFNNI EVLHCERNQL VTLNVCGYFL KALYASSNEL 840

AQLDVYPVPN YLSYMDVSRN CLESVPEWVC ESRKLEVLDI GHNQICELPA RLFCNSSLRK 900

LLAGHNRLAR LPERLERTSV EVLDVQHNQI TELPPNLLMK ADSLRFLNAS ANKLETLPPA 960

TLSEETSSIL QELYLTNNCL TDKCVPLLTG HPRLKILHMA YNRLQSFPAS KMAKLEELEE 1020

IDISGNKLKA IPTTIMNCRR MHTVIAHSNC IEVFPEVMQL PEVKCVDLSC NELSEITLPE 1080

NLPPKLQELD LTGNPRLALD HKSLELLNNI RCFKIDQPSA GDASGAPAVW SHGYTEASGV 1140

KNKLCVAALS VNNFRDNREA LYGVFDGDRN VEVPYLLQCT MSDILAEELQ KTKNEEEYMV 1200

NTFIVMQRKL GTAGQKLGGA AVLCHIKPDP VDLGGSFTLT SANVGKCQTV LCRNGKPLSL 1260

SRSYIMSCEE ERKRIKQHKA IITEDGKVNG VTESTRILGY TFLHPSVVPR PHVQSVLLTP 1320

QDEFFILGSK GLWDSLSIDE AVEAVRNVPD ALAAAKKLCT LAQSYGCHDS ISAVVVQLSV 1380

TEDSFCCCEL SAGGSMPPPS PGIFPPSVNM VIKDRPSDGL GVPSSSSGMA SEISSELSTS 1440

EMSSEVGSTA SDEPPSGVLN ESSPAYPNEQ RCMLHPVCLS NSFQRQLSSA TFSSAFSDNG 1500

LDSDDEEPIE GVFSNGSRVE VEVDIHCSRA KEKERQQHLL QVPAEASDEG IVISANEDES 1560

GLSKKADFSA VGTIGRRRAN GSVAPQERSH NVIEVAADAP LRKPGGYFAA PAQPDPDDQF 1620

IIPPELEEEV KEIMKHHQEQ QQQQQQQQLP PPPQPPQPQP QPQPQPQPQP QRHFQMDHLP 1680

DCYDTPL 1687

Nucleotide Sequence
(FASTA)

ATGGAGCCCG CCGCTGCAGC CCCGGCACAG CGACTCGCGG ACCCCACGGG TGAGGACCAA 60

GCCCTGGCGG CGGCGGCGGC CGAGGGCGGC CGGTGTCCGG ATCCCGCGCT GAGCGCGGCG 120

GCCCCGAGCG GCGGGAACGG CGGCGCGGCG CGAGAGGAGG CCCCCTGCGA GGCGCCGCCG 180

GGGCCGCTGC CCGGCCGCGC CGGAGGCACC GGGCGCAGGC GGCGGCGGGG AGCTCCCCAG 240

CCCGCGGCCG GTGGGGCCGC TCCGGTGCCC GCGGCCGGCG GCGGCGCCAA CTCGCTGCTG 300

CTGAAGAGAG GGCGGCTGAA GAGGAATCTG TCGGCCGCCG CCGCCGCCTC GTCGTCCTCG 360

TCGCCGTCCT CGGCGTCTTC GGCCGCTGGC GGCCTCCCGG CGTCCTGCTC GGCCTCGGCG 420

TCGCTGTGCA CCCGGAGCCT GGACAGGAAG ACGCTGCTCC TGAAGCACCG GCAGCTGCTT 480

CAGCTGCAGC CGTCGGACAG GGACTGGGTG AGGCACCAGC TCCAGCGGGG CTGTGTGCAC 540

GTCTTTGACC GCCACATGGC CTCGTCCTAC CTGCGCCCAG TGCTCTGCAC GCTGGACACC 600

ACGGCCGCCG AGGTGGCGGC CCGGCTTCTG CAGCTGGGCC ACAAAGGCGG CGGGGTGGTG 660

AAGGTGCTGG GCTACGGGCC GCCTCCCGCC GCCGCCCCTG CCGCCAGCGA TCAGACCCTG 720

GACGGAGAGC ACGGAAGGGA CGTGGAGCCA CCGCCCTCGA GCGGCACTGT TGGTGCTGTC 780

CGGGGCCCTG CGCGCGCGCC TCCCGCCGAT CTGCCGCTGC CCGGGGGCGC CTGGACGCGC 840

TGTGCACCCC GCATCAGCCC CGCGCCTTCG GACTCCAGCC CCGGAGAGCT GTTCGCGGGC 900

GGCCCCGGGT CCCCTCCCCG CGCCCCGCGC CCGGCCTCTG ACACCGAGAG CTTCAGCCTG 960

AGCCCCAGCG CCGAGAGCGT GTCAGACCGG CTGGACCCCT ACAGCAGCGG CGGCGGCGGC 1020

TCCTCGTCGT CGTCGGAGGA GTTAGAGGCT GATCCGGCCA TGCCCCACCG CCCCGGCCGC 1080

CCCGCGCAGC CTCGGCCGCC TTCCCCGAAG ACCTCGGCTT TACTGCAGCC GAAAGCCCCG 1140

ACGGGCGTAG ACAGCACTGG CGTGATAGCG GGCGAGGGTC CCGGAGACGA TAAGGCGATG 1200

GCAGCCGCAG CTCCGGACGT TCCTCTGTCG ACTTCGGGGA GGATACGGGA GACCGTGCAA 1260

AAGACATCTC CGCCCTCCCT GTATGTACAG CTCCATGGAG AGACTACCCG GCGCCTGGAG 1320

GCGGACGAGA AGCCATTGCA GATCCAAAAT GACTACCTCT TCCAACTGGG ATTTGGGGAG 1380

CTGTGGAGGG TGCAGGAGGA AGGCATGGAC TCAGAGATTG GCTGTCTCAT TCGATTCTAT 1440

GCAGGAAAGC CTCATAGCAC TGGGAGCTCT GAGCGGATTC AGCTCTCCGG AATGTACAAT 1500

GTCCGAAAAG GCAAAATGCA GTTGCCAGTG AACCGATGGA CAAGACGACA GGTCATTCTG 1560

TGCGGGACGT GCTTGATAGT GTCGTCCGTG AAAGACAGCG TGAGTGGGAA GATGCATGTC 1620

CTGCCCCTCA TTGGTGGAAA AGTGGAGGAA GTGAAAAAGC ACCAGCACTG TTTAGCGTTC 1680

AGCTCTTCGG GGCCTCAAAG CCAGACTTAC TACATCTGCT TTGATACTTT CACGGAGTAC 1740

TTACGGTGGC TGCGGCAAGT CTCCAAGGTT GCATCACAGC GTATAAGCTC AGTAGACCTC 1800

TCCTGTTGTA GCCTTGAACA TCTGCCTGCC AACCTCTTTT ACAGCCAAGA CCTTACTCAT 1860

CTCAACTTAA AACAGAACTT CCTAAGGCAA ACCCCCACCC TCCCAGCTGC CAGAGGACTT 1920

GGTGAACTGC AAAGGTTCAC CAAATTGAAG AGCCTTAACC TTTCCAATAA CCACCTAGGA 1980

GCCTTTCCGT CAGCAGTCTG CAGCATTCCA ACCCTAGCAG AGCTGAATGT ATCTTGCAAT 2040

GCCCTGCGAG AAGTCCCAGC AGCTGTTGGA GATATGCAGA ACTTGCAGAC GTTTCTGCTG 2100

GATGGAAATT TTCTCCAGTC CCTTCCTGCT GAACTGGAGA GCATGCACCA GCTCAGCTAT 2160

CTGGGTCTTT CCTTTAATGA ATTCACTGAC ATTCCAGAGG TATTGGAGAA GCTGACTGCT 2220

GTGGATAAGC TGTGTATGGC TGGGAACTGT GTGGAGACCC TCAGACTACA GGCCTTAAGA 2280

AGAATGCCTC ATATTAAACA TGTGGACCTA AGACTGAACA TACTCAGAAA GCTTATGGCA 2340

GATGAGGTGG ACTTTGTGCA ACATGTCACT CAGCTTGACC TGCGAGACAA TAAGCTTGGT 2400

GATCTAGATG CTATGATCTT CAACAACATA GAAGTTCTGC ACTGTGAGAG GAATCAGCTG 2460

GTGACATTGA ACGTTTGTGG CTATTTCCTA AAGGCACTCT ATGCTTCTTC TAATGAACTT 2520

GCTCAACTTG ATGTCTACCC AGTTCCGAAT TATCTCTCTT ACATGGATGT CTCAAGAAAC 2580

TGCCTAGAAA GTGTGCCTGA GTGGGTATGT GAAAGCCGGA AATTAGAAGT TTTGGATATT 2640

GGCCATAATC AAATATGTGA ACTTCCTGCC CGCCTGTTTT GTAATAGTAG TCTCCGGAAA 2700

TTGCTAGCAG GACACAACCG GTTGGCAAGG CTTCCTGAAA GGCTGGAGAG AACTTCTGTG 2760

GAGGTCTTGG ATGTCCAGCA CAACCAGATC ACTGAGCTCC CACCAAACCT CCTCATGAAG 2820

GCTGACAGCC TGAGATTCCT GAATGCATCT GCAAACAAAC TGGAAACCCT GCCTCCAGCC 2880

ACACTTTCTG AGGAGACGAG CAGTATATTA CAGGAGTTGT ACCTGACAAA CAACTGCCTC 2940

ACGGATAAGT GTGTGCCCTT GTTAACAGGG CACCCCCGTC TGAAGATCCT ACACATGGCC 3000

TATAACCGGC TTCAGAGCTT CCCGGCAAGT AAAATGGCAA AACTGGAGGA ACTTGAAGAA 3060

ATTGATATCA GTGGGAATAA GCTGAAAGCC ATCCCCACAA CAATCATGAA CTGCAGACGC 3120

ATGCACACCG TGATTGCTCA CTCCAATTGT ATCGAAGTCT TTCCTGAAGT TATGCAGCTC 3180

CCAGAAGTCA AGTGTGTAGA TCTGAGCTGT AATGAGCTGA GTGAAATCAC GTTACCAGAA 3240

AACCTGCCAC CTAAGCTGCA GGAGCTAGAC CTGACTGGAA ATCCACGCCT TGCCCTGGAC 3300

CACAAAAGCC TGGAGCTGCT CAATAACATT CGCTGCTTCA AGATTGACCA GCCGTCAGCA 3360

GGAGATGCAT CAGGAGCCCC AGCAGTATGG AGTCATGGTT ACACTGAAGC ATCGGGAGTA 3420

AAAAACAAGC TGTGTGTCGC AGCCCTGTCT GTAAATAACT TCCGTGACAA CCGAGAGGCC 3480

CTCTATGGTG TGTTTGATGG AGACCGGAAT GTGGAGGTGC CCTACCTTCT CCAGTGCACC 3540

ATGAGTGACA TTTTGGCTGA AGAGCTTCAG AAAACGAAAA ATGAAGAAGA ATACATGGTC 3600

AATACATTCA TAGTCATGCA AAGGAAACTG GGAACTGCTG GGCAGAAACT TGGTGGTGCT 3660

GCTGTCCTCT GCCACATCAA GCCTGACCCT GTGGACCTGG GAGGATCCTT TACCCTGACC 3720

TCTGCTAATG TTGGCAAGTG CCAAACGGTT CTCTGTCGAA ATGGGAAGCC ACTGTCTCTG 3780

TCCAGGTCAT ACATCATGAG CTGTGAAGAA GAGCGGAAGA GGATTAAGCA GCACAAAGCC 3840

ATCATCACTG AGGATGGCAA GGTCAATGGA GTGACAGAGT CCACGCGCAT CCTGGGCTAC 3900

ACCTTCCTCC ATCCCAGTGT GGTGCCTCGC CCCCATGTGC AGTCGGTGCT TCTGACTCCA 3960

CAGGATGAGT TTTTCATCCT GGGCAGCAAA GGGCTGTGGG ACAGCCTATC CATTGACGAG 4020

GCTGTGGAAG CTGTACGCAA TGTGCCAGAT GCTCTGGCTG CTGCTAAGAA GCTCTGCACC 4080

CTGGCTCAGA GCTATGGCTG CCATGACAGC ATCAGTGCTG TGGTGGTACA GCTAAGTGTC 4140

ACCGAAGACA GCTTCTGCTG CTGTGAGCTC AGTGCTGGAG GGAGCATGCC ACCACCCAGC 4200

CCCGGAATCT TCCCACCATC CGTCAACATG GTCATCAAGG ACCGACCCTC AGATGGGCTG 4260

GGTGTGCCAT CCTCCAGCAG TGGCATGGCA TCTGAGATCA GCAGTGAACT GTCTACCTCC 4320

GAGATGAGTA GTGAGGTGGG CTCCACAGCC TCTGATGAGC CCCCGTCTGG AGTCCTGAAT 4380

GAGAGCAGCC CTGCCTACCC CAACGAGCAG CGCTGCATGC TCCACCCTGT CTGCCTGTCT 4440

AACTCCTTCC AACGTCAGCT GTCCAGCGCT ACTTTCTCCA GCGCGTTCTC TGACAACGGC 4500

CTTGACAGTG ACGATGAAGA ACCCATTGAG GGGGTGTTCA GCAACGGCAG CCGGGTTGAG 4560

GTGGAAGTAG ACATCCACTG CAGCAGGGCC AAGGAAAAGG AGAGACAGCA GCACCTACTT 4620

CAGGTGCCAG CTGAGGCCAG TGATGAGGGC ATTGTCATCA GTGCCAATGA GGATGAGTCA 4680

GGTCTGTCCA AGAAGGCAGA CTTTTCTGCT GTGGGAACCA TTGGACGACG CAGGGCTAAT 4740

GGCTCTGTAG CTCCCCAGGA AAGGAGCCAT AATGTGATAG AGGTTGCTGC AGATGCGCCT 4800

CTCCGGAAGC CAGGAGGCTA TTTTGCAGCC CCTGCTCAGC CAGATCCAGA TGATCAGTTT 4860

ATCATCCCCC CAGAGCTGGA AGAGGAAGTC AAAGAAATCA TGAAACATCA CCAGGAGCAG 4920

CAGCAGCAGC AGCAGCAGCA GCAGCTGCCA CCACCACCCC AGCCGCCACA GCCACAGCCA 4980

CAGCCACAGC CACAGCCACA GCCACAGCCA CAGCGGCACT TCCAAATGGA TCACCTGCCA 5040

GACTGTTACG ATACACCGCT ATGA 5064

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g+++ +g +n+++ a+ s++nf +++e+     ++gv+DG+++ ev + +++++++il

Q: 1131  SHGYTEASGVKNKLCVAALSVNNFRDNREA-----LYGVFDGDRNVEVPYLLQCTMSDIL  1185

         689**********************99775.....9************************

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikds......  115

         aeel+    ++++ ++++ ++F+ ++++l       ++ +k G +av+++ik++

Q: 1186  AEELQ----KTKNEEEYMVNTFIVMQRKL------GTAGQKLGGAAVLCHIKPDpvdlgg  1235

         ***99....999*****************......99***********************

S: 116   ..klyvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLa  173

           +l+ anvG++++vLcrngk+++ls+ + +s+e+E++Ri+++++ ++++   +v+Gv++

Q: 1236  sfTLTSANVGKCQTVLCRNGKPLSLSRSYIMSCEEERKRIKQHKAIITEDG--KVNGVTE  1293

         999*****************************************9999998..*******

S: 174   vsRalGdlylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvlekke  233

          +R lG ++l+p v+p+P+v+s+ lt++def+iL+s+Glwd +s  eav++vr+v +

Q: 1294  STRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSIDEAVEAVRNVPD---  1350

         *********************************************************...

S: 234   aeqeaakkLvklalargskdnvsaivv  260

          + +aakkL++la+++g++d++sa+vv

Q: 1351  -ALAAAKKLCTLAQSYGCHDSISAVVV  1376

         .************************97

Domain Sequence
(FASTA)

SHGYTEASGV KNKLCVAALS VNNFRDNREA LYGVFDGDRN VEVPYLLQCT MSDILAEELQ 60

KTKNEEEYMV NTFIVMQRKL GTAGQKLGGA AVLCHIKPDP VDLGGSFTLT SANVGKCQTV 120

LCRNGKPLSL SRSYIMSCEE ERKRIKQHKA IITEDGKVNG VTESTRILGY TFLHPSVVPR 180

PHVQSVLLTP QDEFFILGSK GLWDSLSIDE AVEAVRNVPD ALAAAKKLCT LAQSYGCHDS 240

ISAVVV 246

Keyword

Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Leucine-rich repeat; Manganese; Membrane; Metal-binding; Nucleus; Protein phosphatase; Reference proteome; Repeat.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004721	; F:phosphoprotein phosphatase activity
GO:0006915	; P:apoptotic process
GO:0007623	; P:circadian rhythm
GO:0009649	; P:entrainment of circadian clock
GO:0008152	; P:metabolic process

KEGG

mmu:98432

;

InterPros

IPR001611	; Leu-rich_rpt.
IPR011993	; PH_like_dom.
IPR001849	; Pleckstrin_homology.
IPR001932	; PP2C-like.

Pfam

PF00560	; LRR_1; 3.
PF00481	; PP2C; 1.

SMARTs

SM00233	; PH; 1.
SM00332	; PP2Cc; 1.

Prosites

PS51450	; LRR; 17.
PS50003	; PH_DOMAIN; 1.

Prints

Created Date

20-Feb-2013