EPS-MUM-00027

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00027

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	191.7	4.8E-56	230	483	254

Status

Reviewed

Ensembl Protein

ENSMUSP00000061278

UniProt Accession

Q80TL0; Q5SX30; Q8BGM6; Q8CB81;

Protein Name

Protein phosphatase 1E

Protein Synonyms/Alias

Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA;

Gene Name

Ppm1e

Gene Synonyms/Alias

Ppm1e; Camkn, Kiaa1072;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000046442	ENSMUSP00000061278	ENSMUST00000055438

Organism

Mus musculus

Functional Description

Protein phosphatase that inactivates multifunctional CaMkinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42 (By similarity).

Protein Length

749

Protein Sequence
(FASTA)

MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA EAAEASGEEP 60

GEDAATVEAT EEGEQDQDPE PEDEAVEEET ATEGEEEEEE EAAAPGHSAV PPPPQPQLPP 120

LPPLPRPLSE RITREEVEGE SLDLCLQQLY KYNCPSFLAA ALARATSDEV LQSDLSAHCI 180

PKETDGTEGT VEIETVKLAR SVFSKLHEIC CSWVKDFPLR RRPQIYYETS IHAIKNMRRK 240

MEDKHVCIPD FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA 300

EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM LVRKGQAVEL 360

MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI GDAEHKPYIC GDADSASTVL 420

DGTEDYLILA CDGFYDTVNP DEAVKVVSDH LKENNGDSSM VAHKLVASAR DAGSSDNITV 480

IVVFLRDMNK AVNVSEESEW TENSFQGGQE DGGDDKETHG ECKRPWPQHQ CSAPADLGYE 540

GRVDSFTDRT SLSPGPQINV LEDPDYLDLT QIEASKPHST QFLPPVEMIG PGAPKKDLNE 600

LIMEERSVKS SLPERSGAGE PRVSFNLGST GQQICRMENL SPVSSGLENE QFKSRGKTAS 660

RLYHLRHHYS KRQRGFRFNP KFYSFLSARE PSHKIGISLS SLTRSGKRNK MLRSSLPWRE 720

NSWEGYSGNV KIRKRNDIPC PDFPWSYKI 749

Nucleotide Sequence
(FASTA)

ATGGCGGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT GTTCCTGGGC 60

GAGTTCCGCG GACCGTGCGG TGGCGGCGAG CCAGAGCCCG AACCCGAATC CGAGCCCGAG 120

CCCGAGCCCG AAGCCGAGCT AGTCGCGGCT GAGGCGGCCG AGGCTTCGGG GGAGGAACCG 180

GGGGAGGACG CTGCGACGGT AGAGGCGACC GAGGAGGGGG AGCAGGACCA AGACCCCGAG 240

CCCGAAGACG AGGCGGTGGA GGAGGAGACG GCGACGGAGG GCGAGGAGGA GGAAGAGGAG 300

GAAGCGGCTG CCCCGGGGCA CTCGGCCGTG CCGCCACCGC CGCAGCCCCA GCTGCCACCG 360

CTGCCCCCGC TCCCACGGCC GCTGTCGGAG CGCATCACCC GAGAGGAGGT GGAGGGCGAG 420

AGCCTGGACC TGTGCCTGCA GCAGCTCTAC AAATATAACT GCCCTTCCTT TCTGGCTGCT 480

GCTTTAGCCA GAGCCACATC AGATGAAGTC CTCCAGAGTG ACCTTTCTGC TCACTGTATC 540

CCAAAGGAAA CGGACGGCAC AGAAGGGACT GTGGAAATTG AGACAGTGAA ATTGGCCCGT 600

TCTGTCTTCA GCAAACTACA TGAGATCTGC TGCAGTTGGG TGAAGGACTT CCCTCTGCGC 660

AGGAGGCCTC AGATTTATTA TGAGACGTCA ATTCATGCCA TCAAGAACAT GCGAAGGAAA 720

ATGGAGGACA AACACGTCTG CATTCCTGAC TTTAACATGC TCTTCAACCT AGAGGACCAG 780

GAGGAACAAG CATACTTTGC AGTGTTTGAT GGCCATGGGG GAGTGGACGC TGCTATTTAT 840

GCCTCCGTTC ACCTACACGT TAACTTAGTG CGCCAGGAGA TGTTTCCCCA TGATCCTGCT 900

GAGGCTCTCT GCCGGGCCTT CCGTGTCACA GATGAGCGGT TTGTGCAGAA GGCAGCCAGG 960

GAGAGCTTAC GGTGTGGGAC CACAGGAGTA GTGACTTTTA TCAGAGGCAA CATGCTCCAC 1020

GTGGCATGGG TGGGCGATTC CCAGGTCATG CTGGTAAGAA AGGGGCAAGC TGTTGAACTA 1080

ATGAAACCAC ACAAGCCAGA CAGAGAGGAT GAAAAGCAGC GAATTGAGGC CCTTGGAGGC 1140

TGTGTGGTTT GGTTTGGCGC CTGGAGGGTG AATGGAAGCC TGTCCGTCTC CAGAGCCATC 1200

GGAGATGCTG AGCACAAGCC GTATATCTGT GGAGACGCAG ACTCGGCCTC CACTGTTTTG 1260

GATGGGACTG AAGACTACCT CATTCTGGCC TGTGACGGCT TCTATGACAC CGTGAACCCT 1320

GACGAGGCAG TGAAGGTTGT GTCTGACCAC TTGAAAGAGA ATAACGGAGA CAGCAGCATG 1380

GTTGCCCACA AATTAGTAGC GTCGGCTCGT GATGCGGGGT CAAGTGATAA CATCACTGTT 1440

ATTGTGGTAT TCCTGAGGGA CATGAACAAG GCTGTAAATG TTAGTGAGGA GTCAGAGTGG 1500

ACAGAGAACT CTTTTCAAGG AGGGCAAGAA GATGGTGGGG ATGATAAGGA GACTCATGGA 1560

GAGTGCAAGC GCCCTTGGCC ACAGCACCAG TGCTCAGCAC CCGCCGATCT AGGCTATGAG 1620

GGGCGAGTGG ATTCATTCAC TGATAGAACT AGCCTCAGCC CAGGGCCCCA AATCAACGTG 1680

CTGGAAGATC CAGACTACCT AGATCTCACA CAAATAGAAG CAAGCAAACC TCACAGTACC 1740

CAATTTTTGC CACCAGTTGA GATGATTGGT CCTGGTGCAC CAAAGAAAGA TCTTAATGAG 1800

CTAATAATGG AGGAGAGATC AGTCAAGTCA TCATTGCCTG AACGGAGTGG TGCTGGAGAG 1860

CCTCGGGTTT CTTTTAATTT GGGTTCAACA GGGCAACAGA TATGCAGAAT GGAGAACTTG 1920

TCTCCTGTCA GTTCTGGGTT GGAAAATGAA CAGTTCAAAT CCCGGGGGAA GACAGCGTCT 1980

AGGTTGTATC ATTTACGCCA CCACTACTCC AAAAGGCAGC GTGGATTCAG GTTTAATCCA 2040

AAGTTTTACT CGTTTCTGTC TGCTCGAGAG CCTTCTCACA AAATAGGCAT TAGCCTCTCC 2100

TCACTTACTC GAAGTGGGAA GAGAAACAAG ATGTTAAGAA GTTCTTTGCC ATGGAGGGAA 2160

AATAGTTGGG AAGGATATAG TGGAAATGTG AAGATCAGAA AGCGTAATGA TATTCCATGC 2220

CCAGACTTTC CCTGGAGCTA TAAAATTTAA 2250

Domain Profile

S: 4     gvvsvngrrnemedavtsspnflkspekkdr..laffgvyDGhggsevakfcseklheil  61

         ++ +++++r++med++   p+f    + +d+   a+f+v+DGhgg ++a ++s +lh  l

Q: 230   SIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQeeQAYFAVFDGHGGVDAAIYASVHLHVNL  289

         567899**************99766555544459************************99

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdsklyvan  121

          ++         +  +al +aF  tde++  ++++  ++ ++G+t vv  i+++ l+va

Q: 290   VRQEM----FPHDPAEALCRAFRVTDERF--VQKAARESLRCGTTGVVTFIRGNMLHVAW  343

         99955....6788888999**********..7777788888*******************

S: 122   vGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdga.RvlGvLavsRalGd  180

         vGdS+++L r+g+avel + Hkp+redE++Rie+ gg v+ + ga Rv+G+L vsRa+Gd

Q: 344   VGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVV-WFGAwRVNGSLSVSRAIGD  402

         *************************************999.55899**************

S: 181   lylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle.kkeaeqeaa  239

          + kpy+  + +  s+ l+  +++liLa+DG +d+v++ eav++v d+l+ ++ + + +a

Q: 403   AEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKeNNGDSSMVA  462

         ************************************************9966778899**

S: 240   kkLvklalargskdnvsaivv  260

         +kLv+ a++ gs+dn+++ivv

Q: 463   HKLVASARDAGSSDNITVIVV  483

         *****************9997

Domain Sequence
(FASTA)

SIHAIKNMRR KMEDKHVCIP DFNMLFNLED QEEQAYFAVF DGHGGVDAAI YASVHLHVNL 60

VRQEMFPHDP AEALCRAFRV TDERFVQKAA RESLRCGTTG VVTFIRGNML HVAWVGDSQV 120

MLVRKGQAVE LMKPHKPDRE DEKQRIEALG GCVVWFGAWR VNGSLSVSRA IGDAEHKPYI 180

CGDADSASTV LDGTEDYLIL ACDGFYDTVN PDEAVKVVSD HLKENNGDSS MVAHKLVASA 240

RDAGSSDNIT VIVV 254

Keyword

Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Manganese; Metal-binding; Nucleus; Protein phosphatase; Reference proteome; Repeat.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005730	; C:nucleolus
GO:0043234	; C:protein complex
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0035690	; P:cellular response to drug
GO:0006469	; P:negative regulation of protein kinase activity
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0051496	; P:positive regulation of stress fiber assembly

KEGG

mmu:320472

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013