EKS-MUM-00253

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00253

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	338.4	5.7E-101	20	271	252

Status

Reviewed

Ensembl Protein

ENSMUSP00000018971

UniProt Accession

Q69Z98; Q699J3; Q699J4; Q6DMN7; Q6PHM0;

Protein Name

Serine/threonine-protein kinase BRSK2

Protein Synonyms/Alias

Brain-specific serine/threonine-protein kinase 2; BR serine/threonine-protein kinase 2; Serine/threonine-protein kinase SAD-A;

Gene Name

Brsk2

Gene Synonyms/Alias

Brsk2; Kiaa4256, Sada;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000053046	ENSMUSP00000134201	ENSMUST00000174499
ENSMUSG00000053046	ENSMUSP00000077330	ENSMUST00000078200
ENSMUSG00000053046	ENSMUSP00000133296	ENSMUST00000173941
ENSMUSG00000053046	ENSMUSP00000101610	ENSMUST00000105989
ENSMUSG00000053046	ENSMUSP00000074969	ENSMUST00000075528
ENSMUSG00000053046	ENSMUSP00000134153	ENSMUST00000173924
ENSMUSG00000053046	ENSMUSP00000134310	ENSMUST00000174309
ENSMUSG00000053046	ENSMUSP00000018971	ENSMUST00000018971
ENSMUSG00000053046	ENSMUSP00000134170	ENSMUST00000173705
ENSMUSG00000053046	ENSMUSP00000134289	ENSMUST00000174405
ENSMUSG00000053046	ENSMUSP00000133438	ENSMUST00000172652
ENSMUSG00000053046	ENSMUSP00000134566	ENSMUST00000174237
ENSMUSG00000053046	ENSMUSP00000133750	ENSMUST00000172967
ENSMUSG00000053046	ENSMUSP00000133868	ENSMUST00000173313

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase that plays a key role inpolarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apopototic response triggered by endoplasmatic reticulum (ER) stress.

Protein Length

653

Protein Sequence
(FASTA)

MTSTGKDGGG AQHAQYVGPY RLEKTLGKGQ TGLVKLGIHC VTCQKVAIKI VNREKLSESV 60

LMKVEREIAI LKLIEHPHVL KLHDVYENKK YLYLVLEHVS GGELFDYLVK KGRLTPKEAR 120

KFFRQIISAL DFCHSHSICH RDLKPENLLL DERNNIRIAD FGMASLQVGD SLLETSCGSP 180

HYACPEVIRG EKYDGRKADV WSCGVILFAL LVGALPFDDD NLRQLLEKVK RGVFHMPHFI 240

PPDCQSLLRG MIEVDAARRL TLEHIQKHIW YIGGKNEPEP EQPIPRKVQI RSLPSLEDID 300

PDVLDSMHSL GCFRDRNKLL QDLLSEEENQ EKMIYFLLLD RKERYPSHED EDLPPRNEID 360

PPRKRVDSPM LNRHGKRRPE RKSMEVLSVT DGGSPVPARR AIEMAQHGQR SRSISGASSG 420

LSTSPLSSPR VTPHPSPRGS PLPTPKGTPV HTPKESPAGT PNPTPPSSPS VGGVPWRTRL 480

NSIKNSFLGS PRFHRRKLQV PTPEEMSNLT PESSPELAKK SWFGNFINLE KEEQIFVVIK 540

DKPLSSIKAD IVHAFLSIPS LSHSVISQTS FRAEYKATGG PAVFQKPVKF QVDITYTEGG 600

EAQKENGIYS VTFTLLSGPS RRFKRVVETI QAQLLSTHDQ PSAQHLSGII PKS 653

Nucleotide Sequence
(FASTA)

ATGACATCGA CGGGGAAGGA CGGCGGCGGG GCGCAGCACG CGCAGTATGT GGGGCCCTAC 60

CGGCTGGAGA AGACGCTGGG CAAGGGGCAG ACAGGCTTGG TGAAGCTGGG AATCCACTGT 120

GTCACTTGCC AGAAGGTCGC CATCAAAATC GTGAACCGTG AGAAGCTCAG TGAGTCGGTG 180

CTGATGAAGG TGGAGCGAGA GATTGCCATC TTGAAGCTCA TCGAGCATCC ACATGTACTG 240

AAGCTGCATG ACGTCTATGA AAACAAAAAA TATTTATACC TGGTGCTAGA ACATGTGTCT 300

GGGGGAGAGC TGTTCGACTA CCTGGTGAAG AAGGGCCGGC TGACCCCCAA GGAGGCCCGC 360

AAGTTCTTCC GGCAGATCAT CTCTGCACTG GACTTCTGTC ACAGCCACTC CATATGCCAT 420

AGAGACTTGA AGCCAGAGAA CCTGCTGCTA GATGAGAGGA ACAACATCCG TATTGCAGAC 480

TTTGGCATGG CATCCCTGCA GGTGGGAGAC AGCCTGCTGG AGACCAGCTG CGGATCTCCA 540

CACTATGCCT GTCCGGAAGT GATTCGGGGC GAGAAGTATG ATGGCCGCAA GGCAGATGTG 600

TGGAGCTGTG GTGTGATCCT GTTCGCCTTG CTGGTGGGGG CTCTGCCTTT TGATGATGAC 660

AACCTGCGGC AGTTGCTGGA GAAGGTCAAG CGTGGTGTGT TCCACATGCC ACACTTTATC 720

CCACCAGACT GCCAGAGTCT CCTGCGTGGC ATGATTGAGG TGGATGCAGC TCGGCGCCTC 780

ACGCTAGAGC ACATTCAGAA ACACATATGG TATATAGGTG GCAAGAATGA GCCAGAGCCC 840

GAACAGCCCA TCCCACGCAA GGTGCAGATC CGCTCACTAC CCAGCTTGGA AGACATTGAC 900

CCTGATGTGT TGGACAGCAT GCACTCACTG GGCTGCTTCC GAGACCGCAA CAAGCTGCTG 960

CAGGATCTGC TATCTGAGGA GGAGAATCAG GAAAAGATGA TTTATTTCCT CCTCCTGGAT 1020

CGGAAAGAAC GGTATCCAAG CCATGAGGAT GAGGACCTGC CCCCCAGGAA TGAGATAGAC 1080

CCTCCCCGGA AGCGTGTGGA TTCCCCGATG CTGAACCGGC ATGGCAAGCG GCGACCTGAG 1140

CGCAAGTCCA TGGAAGTGCT CAGTGTGACA GATGGTGGCT CCCCAGTGCC TGCACGGAGA 1200

GCCATTGAGA TGGCCCAGCA TGGCCAGAGA TCTCGATCCA TCAGTGGTGC GTCCTCAGGC 1260

CTTTCTACAA GTCCACTCAG CAGTCCTCGG GTGACCCCTC ACCCCTCACC AAGGGGTAGT 1320

CCCCTTCCTA CCCCCAAAGG GACGCCTGTC CACACGCCAA AGGAGAGCCC AGCTGGCACA 1380

CCCAACCCCA CACCACCATC CAGCCCTAGT GTTGGAGGAG TGCCCTGGCG GACACGACTG 1440

AACTCCATCA AGAACAGCTT CCTGGGCTCA CCTCGATTCC ACCGCCGGAA ACTCCAAGTT 1500

CCCACGCCAG AGGAGATGTC CAACCTGACC CCAGAATCCT CTCCAGAGCT GGCCAAGAAA 1560

TCGTGGTTCG GGAACTTCAT CAACCTGGAG AAGGAGGAGC AGATCTTTGT GGTGATCAAG 1620

GACAAGCCCC TGAGCTCCAT CAAGGCTGAC ATCGTTCATG CCTTCCTGTC GATCCCCAGC 1680

CTCAGCCACA GCGTTATTTC CCAGACAAGC TTCAGGGCTG AATACAAGGC CACAGGGGGC 1740

CCAGCAGTGT TCCAGAAGCC GGTCAAGTTC CAGGTGGACA TCACCTACAC TGAGGGCGGA 1800

GAGGCCCAGA AGGAGAATGG CATCTACTCA GTCACATTCA CTTTACTCTC AGGCCCCAGT 1860

CGCCGCTTCA AGAGGGTGGT GGAGACCATC CAGGCCCAGC TGTTAAGCAC CCATGACCAG 1920

CCATCAGCCC AGCACCTGTC AGGAATTATC CCGAAAAGTT AA 1962

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkklseeslekikrEieilkklkhpni  60

         y+l ktlG+g++g Vkl +h  t +kva+Ki++++klse+ l k++rEi+ilk ++hp++

Q: 20    YRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHV  79

         99**********************************************************

S: 61    ikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgiv  120

         +kl++v+e+kk+lylvle++sggeLfdy+++kgrl+ keark+f+q++sa++++hs++i+

Q: 80    LKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSIC  139

         ************************************************************

S: 121   HrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkvD  180

         HrDlKpeNllld+++ni+iaDFG+a+l   ++llet+cGsp+Ya+Pe+++g+kY+g+k+D

Q: 140   HRDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKAD  199

         ************************************************************

S: 181   vWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpekR  240

         vWs+Gv+L+al++g+lPfd++nl++llek+++g +++p+ + ++++sLlr +++vd+++R

Q: 200   VWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARR  259

         ************************************************************

S: 241   atieeilkhewl  252

         +t+e+i+kh w+

Q: 260   LTLEHIQKHIWY  271

         **********95

Domain Sequence
(FASTA)

YRLEKTLGKG QTGLVKLGIH CVTCQKVAIK IVNREKLSES VLMKVEREIA ILKLIEHPHV 60

LKLHDVYENK KYLYLVLEHV SGGELFDYLV KKGRLTPKEA RKFFRQIISA LDFCHSHSIC 120

HRDLKPENLL LDERNNIRIA DFGMASLQVG DSLLETSCGS PHYACPEVIR GEKYDGRKAD 180

VWSCGVILFA LLVGALPFDD DNLRQLLEKV KRGVFHMPHF IPPDCQSLLR GMIEVDAARR 240

LTLEHIQKHI WY 252

Keyword

Alternative splicing; Apoptosis; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Exocytosis; Kinase; Magnesium; Metal-binding; Mitosis; Neurogenesis; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Gallus gallus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Otolemur garnettii"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0050321	; F:tau-protein kinase activity
GO:0007409	; P:axonogenesis
GO:0030010	; P:establishment of cell polarity
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0061178	; P:regulation of insulin secretion involved in cellular response to glucose stimulus

KEGG

mmu:75770

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; FALSE_NEG.

Prints

Created Date

20-Feb-2013