| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00118 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| Other/NAK | 395.8 | 2.4E-118 | 46 | 309 | 264 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000109298 |
| UniProt Accession | Q3UHJ0; B2RUJ0; Q3TY53; Q6ZPZ6; Q80XP6; |
| Protein Name | AP2-associated protein kinase 1 |
| Protein Synonyms/Alias | Adaptor-associated kinase 1; |
| Gene Name | Aak1 |
| Gene Synonyms/Alias | Aak1; Kiaa1048; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Regulates clathrin-mediated endocytosis byphosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity). |
| Protein Length | 352 |
Protein Sequence
(FASTA) | | MKKFFDSRRE QGSSGLGSGS SGGGGSSSGL GSGYIGRVFG IGRQQVTVDE VLAEGGFALV 60 | | FLVRTSNGVK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH KNIVGYIDSS INNVSSGDVW 120 | | EVLILMDFCR GGQVVNLMNQ RLQTGFTENE VLQIFCDTCE AVARLHQCKT PIIHRDLKVE 180 | | NILLHDRGHY VLCDFGSATN KFQNPQAEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT 240 | | TKADIWALGC LLYKLCYFTL PFGESQVAIC DGSFTIPDNS RYSQDMHCLI RYMLEPDPDK 300 | | RPDIYQVSYF SFKLLKKECP VPNVQNSPIP AKLPEPVKAS EAAVKKTQPK AR 352 |
|
Nucleotide Sequence
(FASTA) | | ATGAAGAAGT TTTTCGACTC CAGGCGGGAG CAGGGGAGCT CTGGCCTGGG TTCAGGCTCC 60 | | AGCGGAGGAG GGGGCAGCAG CTCAGGCCTG GGCAGTGGTT ACATCGGAAG GGTCTTCGGC 120 | | ATCGGGAGGC AGCAGGTCAC CGTGGACGAG GTGTTGGCGG AAGGTGGATT CGCTCTTGTC 180 | | TTTCTGGTGA GAACAAGCAA TGGGGTGAAA TGTGCCTTGA AACGTATGTT TGTCAACAAT 240 | | GAACATGACC TCCAGGTGTG CAAGAGGGAG ATCCAGATCA TGAGAGACCT ATCAGGGCAC 300 | | AAGAACATTG TGGGCTACAT TGATTCTAGT ATCAACAATG TGAGCAGCGG CGACGTCTGG 360 | | GAAGTTCTCA TTCTCATGGA CTTCTGCAGA GGAGGCCAGG TGGTAAACCT GATGAACCAG 420 | | CGTCTCCAGA CAGGCTTCAC AGAGAATGAA GTGCTGCAGA TATTCTGTGA CACCTGTGAA 480 | | GCTGTGGCCC GCCTGCATCA GTGCAAAACT CCCATTATCC ACCGGGACCT GAAGGTTGAA 540 | | AATATCCTCT TGCATGACCG AGGCCACTAT GTCTTGTGTG ACTTTGGAAG TGCCACCAAC 600 | | AAATTCCAGA ATCCACAGGC CGAGGGAGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC 660 | | ACAACGCTCT CCTATCGAGC CCCAGAAATG GTCAACTTGT ACAGTGGCAA AATCATCACT 720 | | ACGAAGGCAG ATATTTGGGC TCTAGGCTGT TTGTTGTATA AATTATGCTA CTTCACTTTG 780 | | CCGTTTGGGG AGAGCCAGGT GGCGATTTGT GACGGAAGCT TCACAATTCC TGATAACTCT 840 | | CGTTATTCTC AAGATATGCA CTGCCTTATT AGGTATATGT TGGAACCAGA CCCTGACAAA 900 | | AGGCCGGATA TTTACCAGGT TTCATACTTC TCATTTAAAC TACTCAAGAA AGAATGCCCC 960 | | GTTCCAAATG TACAGAACTC TCCCATTCCT GCAAAACTTC CTGAACCAGT GAAAGCCAGT 1020 | | GAAGCAGCTG TGAAAAAGAC CCAGCCAAAG GCCAGGTAA 1059 |
|
| Domain Profile | S: 1 vriekvlaeGGfalvylaedastgekyalKrilvneeedleeikrEieimkklsghkniv 60 | v++++vlaeGGfalv+l++ +s+g k+alKr++vn+e+dl+ +krEi+im++lsghkniv | Q: 46 VTVDEVLAEGGFALVFLVR-TSNGVKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIV 104 | 6899***************.99************************************** |
| S: 61 klvdssvkeekdngskevlllleyckkgslvdllnkrlekgelseaeilkilldvtrava 120 | ++dss+++ ++++++evl+l+++c+ g++v+l+n+rl++g ++e+e+l+i++d+++ava | Q: 105 GYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTG-FTENEVLQIFCDTCEAVA 163 | ************9***************************9.****************** |
| S: 121 alHalsvpliHRDlkveNillsdegelvlcDlGSattaileveskqelsalqeeiekntt 180 | +lH++++p+iHRDlkveNill+d+g++vlcD+GSat+++++++ +++++a+++ei+k+tt | Q: 164 RLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQ-AEGVNAVEDEIKKYTT 222 | *******************************************.9*************** |
| S: 181 lsyrsPElidlysnlvideksDiWaLGcllYklcffesPfeesvklailngkysiPensr 240 | lsyr+PE+++lys+++i++k+DiWaLGcllYklc+f+ Pf+es ++ai++g+++iP+nsr | Q: 223 LSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGES-QVAICDGSFTIPDNSR 281 | *******************************************.**************** |
| S: 241 yseklkslikkmlevdpeeRptiaqvle 268 | ys+++++li++mle+dp++Rp+i+qv++ | Q: 282 YSQDMHCLIRYMLEPDPDKRPDIYQVSY 309 | **************************87 |
|
Domain Sequence
(FASTA) | | VTVDEVLAEG GFALVFLVRT SNGVKCALKR MFVNNEHDLQ VCKREIQIMR DLSGHKNIVG 60 | | YIDSSINNVS SGDVWEVLIL MDFCRGGQVV NLMNQRLQTG FTENEVLQIF CDTCEAVARL 120 | | HQCKTPIIHR DLKVENILLH DRGHYVLCDF GSATNKFQNP QAEGVNAVED EIKKYTTLSY 180 | | RAPEMVNLYS GKIITTKADI WALGCLLYKL CYFTLPFGES QVAICDGSFT IPDNSRYSQD 240 | | MHCLIRYMLE PDPDKRPDIY QVSY 264 |
|
| Keyword | Alternative splicing; ATP-binding; Cell membrane; Coated pit; Complete proteome; Endocytosis; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |