EKS-MUM-00120

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00120

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NAK	359.7	2.4E-107	41	313	273

Status

Reviewed

Ensembl Protein

ENSMUSP00000036705

UniProt Accession

Q99KY4; Q6P1I8; Q6P9S5; Q8BM74; Q8K0Q4;

Protein Name

Cyclin-G-associated kinase

Protein Synonyms/Alias

Gene Name

Gak

Gene Synonyms/Alias

Gak;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000062234	ENSMUSP00000115184	ENSMUST00000156110
ENSMUSG00000062234	ENSMUSP00000116862	ENSMUST00000139303
ENSMUSG00000062234	ENSMUSP00000118713	ENSMUST00000145467
ENSMUSG00000062234	ENSMUSP00000036705	ENSMUST00000046603
ENSMUSG00000062234	ENSMUSP00000118008	ENSMUST00000135225

Organism

Mus musculus

Functional Description

Associates with cyclin G and CDK5. Seems to act as anauxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).

Protein Length

1305

Protein Sequence
(FASTA)

MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDL 60

GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 120

LLTELCKGQL VEFLKRVECK GPLSCDSILK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 180

LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI 240

GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP 300

EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC GGTVNSSGVL 360

ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN YAKGDLDISY ITSRIAVMSF 420

PAEGVESAIK NNIEDVRMFL DAKHPGHYAV YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL 480

HSLYTLCRSM HAWLREDHRN VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK 540

RCPPGIWPSH KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 600

VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG GRLQAKMASM 660

KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ VNLEVEVEPR DRPSREAPPW 720

ENTSLRGLNP KILFSNREEQ QDILSKFGKP ELPRQPGSTA QYDAEAGSPE AEITESDSPQ 780

SSSTDTNHFL HTLDWQEEKE PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK 840

PGAGEDTPRL AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG 900

VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ GKVPDTVDPF 960

DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS APPPSCSTAF LHLGDLPAEP 1020

SKVIASSSHP DLLGGWDTWA DTATPGPASI PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD 1080

PFADLSDLSS SLQGLPAGLP AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA 1140

SEQLRSHFSV IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 1200

QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD LVTPEQVKKQ 1260

YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG SRPLF 1305

Nucleotide Sequence
(FASTA)

ATGTCGCTGC TGCAGTCTGC GCTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 60

GCGGGCCGTG ATCAGAGTGA CTTCGTGGGG CAGACAGTGG AGCTGGGCGA GCTGCGTCTG 120

CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCTTTTG TTTATGAAGC TCAAGATCTG 180

GGAAGTGGCA GAGAGTATGC ATTAAAGCGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 240

GCCATCATTC AGGAAGTTTG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 300

TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAGTCGGACA CTGGGCAGGC TGAGTTCCTC 360

CTGCTTACGG AGCTTTGTAA AGGACAGCTG GTGGAGTTTC TCAAGAGAGT TGAATGTAAA 420

GGTCCTCTGT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 480

CACATGCACA GGCAGAAACC ACCCATCATC CACAGAGATC TCAAGGTTGA AAACTTACTG 540

CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 600

TATCCTGACT ACAGCTGGAG TGCCCAGAAG CGAGCCATGG TGGAGGAAGA GATCACAAGG 660

AACACCACAC CCATGTACAG AACACCAGAA ATTGTAGACC TGTATTCCAA CTTCCCCATT 720

GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 780

CATCCTTTTG AGGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 840

AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG CCATGCTAAA GGTCAACCCA 900

GAGGAGAGGC TGTCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCTAGG 960

AATGTGAACC CCAAAGCTCC CATCACAGAG CTGCTGGAGC AGAATGGTGG CTATGGGAAC 1020

TCAGGGCCTT CCCGAGCACA ACCACCTTGC GGGGGCACCG TGAACAGCAG TGGAGTTTTG 1080

GCTCTGGCAG AGTATGACCA GCCCTACGGT GGGTTTCTTG ATATTCTGCG GGGTGGGACC 1140

GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 1200

TATGCAAAGG GTGATCTGGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCATTC 1260

CCAGCAGAAG GTGTGGAGTC TGCAATCAAA AACAATATAG AGGATGTGCG AATGTTTCTG 1320

GATGCTAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGGAT ATACCGGGCT 1380

TCCAAGTTTC ACAACCGGGT CACTGAGTGT GGCTGGGCAG TCAGACGGGC ACCACATCTC 1440

CACAGTTTGT ATACTCTATG TAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 1500

GTCTGTGTTG TGCATTGCAT GGATGGGAGA GCTGCATCTG CTGTGGCAGT CTGTGCATTC 1560

CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 1620

CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATATGT ATGTGACATG 1680

GTGGCAGAGG AGCCCATCAC GCCCCATAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 1740

ACCCCCGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCGTTCTG TGAGGTCTAC 1800

GTTGGAGAGG AGCGTGTCAC CACCACGTCG CAGGAATATG ACAGAATGAA GGAATTTAAA 1860

ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCGTAACAG TTCAAGGAGA CGTGCTCATC 1920

ATCATCTACC ATGCCAGGGC CACACTGGGA GGGAGGCTGC AGGCTAAGAT GGCGTCCATG 1980

AAAATGTTCC AGATCCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 2040

TTTGCAAAAT ATGACCTGGA TGCTTGTGAT ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 2100

GTGAACCTGG AAGTGGAGGT AGAGCCTAGA GACAGGCCCA GCCGAGAAGC TCCACCTTGG 2160

GAGAACACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAG 2220

CAGGACATTC TGTCTAAGTT TGGGAAGCCG GAGCTACCCC GGCAGCCGGG CTCCACAGCT 2280

CAGTATGATG CTGAGGCAGG GTCTCCGGAG GCTGAGATCA CAGAGTCGGA CTCACCGCAG 2340

AGCAGCAGTA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAA 2400

CCAGAGACTG GGTTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTGTCCT GATTGCAGAC 2460

GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT TCCCCAGTGA GGAGAGGAAA 2520

CCAGGAGCTG GAGAAGATAC ACCAAGGCTG GCTGCTGGGA CCAAACAGCA AGACTTAATA 2580

TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCCGAAGA AGGTGTTGAT 2640

CTCCTGGGGC TACACTCTGA GGGGGACTTA AGGCCTGCTG CCCCCTTACA GGCTTGTGGG 2700

GTCCCATCTA GCAACACTGA CCTGTTGAGC TGCCTTCTTG AACCATCTGA TGCTGCTCAA 2760

GTAGGGCCTC CTGGTGACCT GCTTGGTGGT GAGGCTCCAC TGCTGCTAGC AAGCCCAGTT 2820

TCTCCTCTTG GGCTGCAGAA CAACCTACAA GGAAAAGTCC CTGACACTGT GGACCCATTT 2880

GACCAGTTCC TGCTGTCATC CAACTCAGAC ACCCAGCCCT GCTCCAAGCC TGATCTCTTT 2940

GGAGAGTTTC TCAACTCTGA CTCTGTAGCT TCCTCAACTG CCTTCCCATC CACCCACAGT 3000

GCCCCACCCC CATCCTGTAG CACTGCCTTC CTGCACCTAG GGGATCTGCC AGCAGAGCCC 3060

AGCAAGGTAA TAGCTTCATC CAGCCACCCA GATCTGCTAG GAGGATGGGA TACGTGGGCT 3120

GACACAGCTA CACCTGGGCC AGCCTCCATT CCAGTACCAG AAGGTACCCT CTTCTCTTCT 3180

GCAGGTCACC CAGCCCCTCC TGGCCCCAAC CCCAGCCAAA CCAAGTCTCA GAACCTTGAC 3240

CCATTTGCTG ATCTCAGTGA TCTCAGCTCT AGCCTCCAAG GCCTGCCTGC TGGACTTCCT 3300

GCAGGGGGCT TTGTTGGCGC ACCAGCCCCC ACTCAAAAGA GCAACAGCCC CTGGCAGGCA 3360

AATCGTCCCA CAGCCCCTGG AACCTCATGG ACCCCCCAGG CCAAGCCAGC CCCCAGAGCC 3420

TCTGAACAGC TAAGGTCTCA CTTCAGTGTG ATCGGGGCCC GAGAAGAGAG GGGCGTCCGT 3480

GTGCCCAGCT TTGCCCAAAA GCCAAAGGTC TCAGAAAATG ATTTTGAAGA TCTTCTGCCT 3540

AATCAAGGCT TCTCTAAGTC TGACAAGAAG GGGCCAAAGA CCATGGCAGA GATGCGGAAA 3600

CAGGAACTTG CCAGAGATAC AGACCCACTC AAATTGAAGC TTTTGGACTG GATTGAAGGC 3660

AAGGAGAGGA ACATTCGTGC ACTGCTGTCC ACTCTGCACA CGGTATTGTG GGATGGGGAG 3720

AGCCGCTGGA CACCTGTGAG TATGGCTGAC CTGGTGACTC CAGAGCAGGT GAAGAAGCAG 3780

TACCGCCGTG CAGTGCTGGT AGTGCATCCT GATAAGGCCA CAGGGCAGCC GTATGAACAG 3840

TATGCCAAGA TGATCTTCAT GGAGCTGAAT GATGCATGGT CTGAGTTTGA AAACCAGGGC 3900

TCGAGGCCCC TCTTCTGA 3918

Domain Profile

S: 2     riekvlaeGGfalvylaedastgekyalKrilvneeedleeikrEieimkklsghknivk  61

         r+++vlaeGGfa+vy a+d+ +g++yalKr+l+neee++++i++E++++kklsgh+niv+

Q: 41    RVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQ  100

         89**********************************************************

S: 62    lv..dssvkeekdngskevlllleyckkgslvdllnkrlekgelseaeilkilldvtrav  119

         ++  +s  kee+d+g++e+lll+e+ck g+lv++l++ + kg ls+++ilki+++++rav

Q: 101   FCsaASIGKEESDTGQAEFLLLTELCK-GQLVEFLKRVECKGPLSCDSILKIFYQTCRAV  159

         **877888999****************.8*******************************

S: 120   aalHalsvpliHRDlkveNillsdegelvlcDlGSattaileve...skqelsalqeeie  176

         +++H++++p+iHRDlkveN+lls++g+++lcD+GSatt+++ ++   s+q++++++eei+

Q: 160   QHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDyswSAQKRAMVEEEIT  219

         *******************************************99999************

S: 177   knttlsyrsPElidlysnlvideksDiWaLGcllYklcffesPfeesvklailngkysiP  236

         +ntt++yr+PE++dlysn++i+ek+DiWaLGc+lY lcf+++Pfe+++kl+i+ngkysiP

Q: 220   RNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIP  279

         ************************************************************

S: 237   ensryseklkslikkmlevdpeeRptiaqvlell  270

          n++ ++ +++li++ml+v+peeR++ia+v+++l

Q: 280   VNDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQL  313

         ******************************9875

Domain Sequence
(FASTA)

RVRRVLAEGG FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ 60

FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKRVECK GPLSCDSILK IFYQTCRAVQ 120

HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR 180

NTTPMYRTPE IVDLYSNFPI GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV 240

NDTRYTVFHD LIRAMLKVNP EERLSIAEVV RQL 273

Keyword

Alternative splicing; ATP-binding; Cell cycle; Cell junction; Complete proteome; Cytoplasm; Golgi apparatus; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005925	; C:focal adhesion
GO:0005794	; C:Golgi apparatus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle

KEGG

mmu:231580

;

InterPros

IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR001623	; DnaJ_N.
IPR011009	; Kinase-like_dom.
IPR014019	; Phosphatase_tensin-typ.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR014020	; Tensin_phosphatase_C2-dom.

Pfam

PF00226	; DnaJ; 1.
PF00069	; Pkinase; 1.
PF10409	; PTEN_C2; 1.

SMARTs

SM00271

; DnaJ; 1.

Prosites

PS51182	; C2_TENSIN; 1.
PS00636	; DNAJ_1; FALSE_NEG.
PS50076	; DNAJ_2; 1.
PS51181	; PPASE_TENSIN; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013