EKS-MUM-00330

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00330

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/EGFR	495.9	5.6E-149	721	978	258

Status

Reviewed

Ensembl Protein

ENSMUSP00000053897

UniProt Accession

P70424; Q61525; Q6ZPE0;

Protein Name

Receptor tyrosine-protein kinase erbB-2

Protein Synonyms/Alias

Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2;

Gene Name

Erbb2

Gene Synonyms/Alias

Erbb2; Kiaa3023, Neu;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000062312	ENSMUSP00000053897	ENSMUST00000058295

Organism

Mus musculus

Functional Description

Protein tyrosine kinase that is part of several cellsurface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). In the nucleus is involved in transcriptionalregulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth (By similarity).

Protein Length

1256

Protein Sequence
(FASTA)

MELAAWCRWG FLLALLSPGA AGTQVCTGTD MKLRLPASPE THLDMLRHLY QGCQVVQGNL 60

ELTYLPANAS LSFLQDIQEV QGYMLIAHNR VKHVPLQRLR IVRGTQLFED KYALAVLDNR 120

DPLDNVTTAA PGRTPEGLRE LQLRSLTEIL KGGVLIRGNP QLCYQDMVLW KDVLRKNNQL 180

APVDMDTNRS RACPPCAPTC KDNHCWGESP EDCQILTGTI CTSGCARCKG RLPTDCCHEQ 240

CAAGCTGPKH SDCLACLHFN HSGICELHCP ALITYNTDTF ESMLNPEGRY TFGASCVTTC 300

PYNYLSTEVG SCTLVCPPNN QEVTAEDGTQ RCEKCSKPCA GVCYGLGMEH LRGARAITSD 360

NIQEFAGCKK IFGSLAFLPE SFDGNPSSGV APLKPEHLQV FETLEEITGY LYISAWPESF 420

QDLSVFQNLR VIRGRILHDG AYSLTLQGLG IHSLGLRSLR ELGSGLALIH RNTHLCFVNT 480

VPWDQLFRNP HQALLHSGNR PEEACGLEGL VCNSLCARGH CWGPGPTQCV NCSQFLRGQE 540

CVEECRVWKG LPREYVRGKH CLPCHPECQP QNSSETCYGS EADQCEACAH YKDSSSCVAR 600

CPSGVKPDLS YMPIWKYPDE EGICQPCPIN CTHSCVDLDE RGCPAEQRAS PVTFIIATVV 660

GVLLFLIIVV VIGILIKRRR QKIRKYTMRR LLQETELVEP LTPSGAVPNQ AQMRILKETE 720

LRKLKVLGSG AFGTVYKGIW IPDGENVKIP VAIKVLRENT SPKANKEILD EAYVMAGVGS 780

PYVSRLLGIC LTSTVQLVTQ LMPYGCLLDH VREHRGRLGS QDLLNWCVQI AKGMSYLEEV 840

RLVHRDLAAR NVLVKSPNHV KITDFGLARL LDIDETEYHA DGGKVPIKWM ALESILRRRF 900

THQSDVWSYG VTVWELMTFG AKPYDGIPAR EIPDLLEKGE RLPQPPICTI DVYMIMVKCW 960

MIDSECRPRF RELVSEFSRM ARDPQRFVVI QNEDLGPSSP MDSTFYRSLL EDDDMGELVD 1020

AEEYLVPQQG FFSPDPALGT GSTAHRRHRS SSARSGGGEL TLGLEPSEEE PPRSPLAPSE 1080

GAGSDVFDGD LAVGVTKGLQ SLSPHDLSPL QRYSEDPTLP LPPETDGYVA PLACSPQPEY 1140

VNQPEVRPQS PLTPEGPPPP IRPAGATLER PKTLSPGKNG VVKDVFAFGG AVENPEYLAP 1200

RAGTASQPHP SPAFSPAFDN LYYWDQNSSE QGPPPSTFEG TPTAENPEYL GLDVPV 1256

Nucleotide Sequence
(FASTA)

ATGGAGCTGG CGGCCTGGTG CCGTTGGGGG TTCCTCCTCG CCCTCCTGTC CCCCGGAGCC 60

GCGGGTACCC AAGTGTGTAC CGGTACCGAC ATGAAGTTGC GACTCCCTGC CAGTCCTGAG 120

ACCCACCTGG ACATGCTTCG CCACCTCTAC CAGGGCTGTC AGGTGGTGCA GGGCAATTTG 180

GAGCTTACCT ACCTGCCCGC CAATGCCAGC CTCTCATTCC TGCAGGACAT CCAGGAAGTC 240

CAGGGATACA TGCTCATCGC TCACAACCGA GTGAAACACG TCCCACTGCA GAGGTTGCGC 300

ATCGTGAGAG GGACTCAGCT CTTTGAGGAC AAGTATGCCC TGGCTGTGCT AGACAACCGA 360

GACCCTTTGG ACAACGTCAC CACCGCCGCC CCAGGCAGAA CCCCAGAAGG GCTGCGGGAG 420

CTGCAGCTTC GAAGTCTCAC AGAGATCTTG AAGGGAGGAG TTTTGATCCG TGGGAACCCT 480

CAGCTCTGCT ACCAGGACAT GGTTTTGTGG AAGGATGTCC TCCGTAAGAA TAACCAGCTG 540

GCTCCTGTCG ACATGGACAC CAATCGTTCC CGGGCCTGTC CACCTTGTGC CCCAACCTGC 600

AAAGACAATC ACTGTTGGGG TGAGAGTCCT GAAGACTGTC AGATCTTGAC TGGCACCATC 660

TGTACTAGTG GCTGTGCCCG GTGCAAGGGC CGGCTGCCCA CTGACTGTTG CCATGAGCAG 720

TGTGCTGCAG GCTGCACGGG TCCCAAGCAT TCTGACTGCC TGGCCTGCCT CCACTTCAAT 780

CATAGTGGTA TCTGTGAGCT GCACTGCCCG GCCCTCATCA CCTACAACAC AGACACCTTC 840

GAGTCCATGC TCAACCCTGA GGGTCGCTAC ACCTTTGGTG CCAGCTGTGT GACCACCTGC 900

CCCTACAACT ACCTCTCCAC GGAAGTGGGA TCCTGCACTC TGGTCTGTCC CCCGAACAAC 960

CAAGAGGTCA CAGCTGAGGA CGGAACACAG CGGTGTGAGA AATGCAGCAA GCCCTGTGCT 1020

GGAGTATGCT ATGGTCTGGG CATGGAGCAC CTCCGAGGGG CGAGGGCCAT CACCAGTGAC 1080

AATATCCAGG AGTTTGCTGG CTGCAAGAAG ATCTTTGGGA GCCTGGCATT TTTGCCGGAG 1140

AGCTTTGATG GGAACCCCTC CTCCGGCGTT GCCCCACTGA AGCCAGAGCA TCTCCAAGTG 1200

TTCGAAACCC TGGAGGAGAT CACAGGTTAC CTATACATTT CAGCATGGCC AGAGAGCTTC 1260

CAAGACCTCA GTGTCTTCCA GAACCTTCGG GTCATTCGGG GACGGATTCT CCATGATGGT 1320

GCTTACTCAT TGACGTTGCA AGGCCTGGGG ATTCACTCAC TGGGGCTACG CTCACTGCGG 1380

GAGCTGGGCA GTGGATTGGC TCTCATTCAC CGCAACACCC ATCTCTGCTT TGTAAACACT 1440

GTACCTTGGG ACCAGCTCTT CCGGAACCCG CACCAGGCCC TACTCCACAG TGGGAACCGG 1500

CCAGAAGAGG CATGTGGTCT TGAGGGCTTG GTCTGTAACT CACTGTGTGC CCGTGGGCAC 1560

TGCTGGGGGC CAGGGCCCAC CCAGTGTGTC AACTGCAGTC AGTTCCTCCG GGGCCAGGAG 1620

TGTGTGGAGG AGTGCCGAGT ATGGAAGGGG CTCCCCAGGG AGTATGTGAG GGGCAAGCAC 1680

TGTCTGCCAT GCCACCCCGA GTGTCAGCCT CAAAACAGCT CGGAGACCTG CTATGGATCG 1740

GAGGCTGACC AGTGTGAGGC TTGTGCCCAC TACAAGGACT CATCTTCCTG TGTGGCTCGC 1800

TGCCCCAGTG GTGTGAAGCC AGACCTCTCC TACATGCCTA TCTGGAAGTA CCCGGATGAG 1860

GAGGGCATAT GTCAGCCATG CCCCATCAAC TGCACCCACT CATGTGTGGA CCTGGACGAA 1920

CGAGGCTGCC CAGCAGAGCA GAGAGCCAGC CCAGTGACAT TCATCATTGC AACTGTGGTG 1980

GGCGTCCTGT TGTTCCTGAT CATAGTGGTG GTCATTGGAA TCCTAATCAA ACGAAGGCGA 2040

CAGAAGATCC GGAAGTATAC CATGCGTAGG CTGCTGCAGG AGACCGAGCT GGTGGAGCCG 2100

CTGACGCCCA GTGGAGCTGT GCCCAACCAG GCTCAGATGC GGATCCTAAA GGAGACAGAG 2160

CTAAGGAAGC TGAAGGTGCT TGGGTCAGGA GCCTTCGGCA CTGTCTACAA GGGCATCTGG 2220

ATCCCAGATG GGGAGAACGT GAAAATCCCC GTGGCCATCA AGGTGTTGAG GGAAAACACA 2280

TCTCCTAAAG CTAACAAAGA AATCCTAGAT GAAGCGTACG TCATGGCTGG TGTGGGTTCT 2340

CCATATGTGT CCCGCCTCCT GGGCATCTGC CTGACATCCA CAGTGCAGCT GGTGACACAG 2400

CTTATGCCCT ATGGCTGCCT TCTGGACCAT GTCCGAGAAC ACCGAGGTCG CTTAGGCTCC 2460

CAGGACCTGC TCAACTGGTG TGTTCAGATT GCCAAGGGGA TGAGCTACCT GGAGGAAGTT 2520

CGGCTTGTTC ACAGGGACCT AGCTGCCCGA AACGTGCTAG TCAAGAGTCC CAACCACGTC 2580

AAGATTACCG ACTTCGGGCT GGCACGGCTG CTGGACATTG ATGAGACTGA ATACCATGCA 2640

GATGGGGGCA AGGTGCCCAT CAAGTGGATG GCATTGGAAT CTATTCTCAG ACGCCGGTTC 2700

ACCCATCAGA GTGATGTGTG GAGCTATGGT GTGACTGTGT GGGAGCTGAT GACCTTTGGG 2760

GCCAAACCTT ACGATGGGAT CCCAGCTCGG GAGATCCCTG ATTTGCTGGA GAAGGGAGAA 2820

CGCCTACCTC AGCCTCCAAT CTGCACCATC GACGTCTACA TGATCATGGT CAAATGTTGG 2880

ATGATTGACT CCGAATGTCG CCCGAGATTC CGGGAGTTGG TATCAGAATT CTCCCGTATG 2940

GCAAGGGACC CCCAGCGCTT TGTGGTCATC CAGAACGAGG ACTTAGGCCC CTCCAGCCCC 3000

ATGGACAGCA CCTTCTACCG TTCACTGCTG GAGGATGATG ACATGGGGGA GCTGGTCGAT 3060

GCTGAAGAGT ACCTGGTACC CCAGCAGGGA TTCTTCTCCC CAGACCCTGC CCTAGGTACT 3120

GGGAGCACAG CCCACCGCAG ACACCGCAGC TCGTCGGCCA GGAGTGGCGG TGGTGAGCTG 3180

ACACTGGGCC TGGAGCCCTC GGAAGAAGAG CCCCCCAGAT CTCCACTGGC TCCCTCCGAA 3240

GGGGCTGGCT CCGATGTGTT TGATGGTGAC CTGGCAGTGG GGGTAACCAA AGGACTGCAG 3300

AGCCTCTCTC CACATGACCT CAGCCCTCTA CAGCGGTACA GTGAGGATCC CACATTACCT 3360

CTGCCCCCCG AGACTGATGG CTACGTTGCT CCCCTGGCCT GCAGCCCCCA GCCCGAGTAT 3420

GTGAACCAGC CAGAGGTTCG GCCTCAGTCT CCCTTGACCC CAGAGGGTCC TCCGCCTCCC 3480

ATCCGACCTG CTGGTGCTAC TCTAGAAAGA CCCAAGACTC TCTCTCCTGG GAAAAATGGG 3540

GTTGTCAAAG ACGTTTTTGC CTTTGGGGGT GCTGTGGAGA ACCCTGAATA CTTAGCACCC 3600

AGAGCAGGCA CTGCCTCTCA GCCCCACCCT TCTCCTGCCT TCAGCCCAGC CTTTGACAAC 3660

CTCTATTACT GGGACCAGAA CTCATCGGAG CAGGGTCCTC CACCAAGTAC CTTTGAAGGG 3720

ACCCCCACTG CAGAGAACCC TGAGTACCTA GGCCTGGATG TGCCAGTATG A 3771

Domain Profile

S: 1     lkklkvlGsGafGtvykGiwipegenvkipvaikvleektgkkankelldealvlasldh  60

         l+klkvlGsGafGtvykGiwip+genvkipvaikvl+e+t++kanke+ldea+v+a + +

Q: 721   LRKLKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGS  780

         69**********************************************************

S: 61    anlvrllglclsstlqlvtqllplGclldyvrehkdklGsqellnwcvqiakGmsyleek  120

         ++++rllg+cl+st+qlvtql+p+Gclld+vreh+++lGsq+llnwcvqiakGmsylee

Q: 781   PYVSRLLGICLTSTVQLVTQLMPYGCLLDHVREHRGRLGSQDLLNWCVQIAKGMSYLEEV  840

         ************************************************************

S: 121   rlvhrdlaarnvlvkspnhvkitdfGlaklleadekelkaeggkvpikwlalesilkrky  180

         rlvhrdlaarnvlvkspnhvkitdfGla+ll+ de+e++a+ggkvpikw+alesil+r++

Q: 841   RLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRF  900

         ************************************************************

S: 181   thqsdvwsyGvtvwelltfGakpydgipakeipdllekGerlaqppictidvymvlvkcw  240

         thqsdvwsyGvtvwel+tfGakpydgipa+eipdllekGerl+qppictidvym++vkcw

Q: 901   THQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCW  960

         ************************************************************

S: 241   midaesrpkfkelaeefs  258

         mid+e+rp+f+el++efs

Q: 961   MIDSECRPRFRELVSEFS  978

         ****************96

Domain Sequence
(FASTA)

LRKLKVLGSG AFGTVYKGIW IPDGENVKIP VAIKVLRENT SPKANKEILD EAYVMAGVGS 60

PYVSRLLGIC LTSTVQLVTQ LMPYGCLLDH VREHRGRLGS QDLLNWCVQI AKGMSYLEEV 120

RLVHRDLAAR NVLVKSPNHV KITDFGLARL LDIDETEYHA DGGKVPIKWM ALESILRRRF 180

THQSDVWSYG VTVWELMTFG AKPYDGIPAR EIPDLLEKGE RLPQPPICTI DVYMIMVKCW 240

MIDSECRPRF RELVSEFS 258

Keyword

Activator; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Disulfide bond; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Receptor; Reference proteome; Signal; Transcription; Transcription regulation; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016324	; C:apical plasma membrane
GO:0009925	; C:basal plasma membrane
GO:0005737	; C:cytoplasm
GO:0010008	; C:endosome membrane
GO:0016021	; C:integral to membrane
GO:0043219	; C:lateral loop
GO:0045121	; C:membrane raft
GO:0005902	; C:microvillus
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0045211	; C:postsynaptic membrane
GO:0043235	; C:receptor complex
GO:0005524	; F:ATP binding
GO:0004716	; F:receptor signaling protein tyrosine kinase activity
GO:0001042	; F:RNA polymerase I core binding
GO:0004714	; F:transmembrane receptor protein tyrosine kinase activity
GO:0060209	; P:estrus
GO:0010001	; P:glial cell differentiation
GO:0007507	; P:heart development
GO:0001889	; P:liver development
GO:0060056	; P:mammary gland involution
GO:0008045	; P:motor neuron axon guidance
GO:0042552	; P:myelination
GO:0043066	; P:negative regulation of apoptotic process
GO:0033088	; P:negative regulation of immature T cell proliferation in thymus
GO:0007528	; P:neuromuscular junction development
GO:0007422	; P:peripheral nervous system development
GO:0014065	; P:phosphatidylinositol 3-kinase cascade
GO:0045785	; P:positive regulation of cell adhesion
GO:0030307	; P:positive regulation of cell growth
GO:0050679	; P:positive regulation of epithelial cell proliferation
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0043410	; P:positive regulation of MAPK cascade
GO:0014068	; P:positive regulation of phosphatidylinositol 3-kinase cascade
GO:0001934	; P:positive regulation of protein phosphorylation
GO:0046579	; P:positive regulation of Ras protein signal transduction
GO:0032321	; P:positive regulation of Rho GTPase activity
GO:0045943	; P:positive regulation of transcription from RNA polymerase I promoter
GO:0045945	; P:positive regulation of transcription from RNA polymerase III promoter
GO:0045727	; P:positive regulation of translation
GO:0046777	; P:protein autophosphorylation
GO:0070372	; P:regulation of ERK1 and ERK2 cascade
GO:0032886	; P:regulation of microtubule-based process
GO:0048678	; P:response to axon injury
GO:0042493	; P:response to drug
GO:0032570	; P:response to progesterone stimulus
GO:0023014	; P:signal transduction by phosphorylation
GO:0048485	; P:sympathetic nervous system development
GO:0043586	; P:tongue development
GO:0006351	; P:transcription, DNA-dependent
GO:0007169	; P:transmembrane receptor protein tyrosine kinase signaling pathway
GO:0042060	; P:wound healing

KEGG

mmu:13866

;

InterPros

IPR021157	; Cyt_c1_TM_anchor_C.
IPR000494	; EGF_rcpt_L.
IPR006211	; Furin-like_Cys-rich_dom.
IPR006212	; Furin_repeat.
IPR009030	; Growth_fac_rcpt.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016245	; Tyr_kinase_EGF/ERB/XmrK_rcpt.

Pfam

PF00757	; Furin-like; 1.
PF07714	; Pkinase_Tyr; 1.
PF01030	; Recep_L_domain; 2.

SMARTs

SM00261	; FU; 4.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013