EKS-MUM-00345

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00345

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE20	351.9	5.3E-105	24	274	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000078746

UniProt Accession

Q99KH8;

Protein Name

Serine/threonine-protein kinase 24 12 kDa subunit

Protein Synonyms/Alias

Mammalian STE20-like protein kinase 3; MST-3; STE20-like kinase MST3; Mammalian STE20-like protein kinase 3 N-terminal; MST3/N; Mammalian STE20-like protein kinase 3 C-terminal; MST3/C;

Gene Name

Stk24

Gene Synonyms/Alias

Stk24; Mst3, Stk3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000063410	ENSMUSP00000078746	ENSMUST00000079817

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase that acts on both serineand threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress- induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase- independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. Acts as a key regulator of axon regeneration in the optic nerve and radial nerve (By similarity).

Protein Length

431

Protein Sequence
(FASTA)

MAHSPVQSGL PGMQNLKADP EELFTKLEKI GKGSFGEVFK GIDNRTQKVV AIKIIDLEEA 60

EDEIEDIQQE ITVLSQCDSP YVTKYYGSYL KDTKLWIIME YLGGGSALDL LEPGPLDEIQ 120

IATILREILK GLDYLHSEKK IHRDIKAANV LLSEHGEVKL ADFGVAGQLT DTQIKRNTFV 180

GTPFWMAPEV IKQSAYDSKA DIWSLGITAI ELAKGEPPHS ELHPMKVLFL IPKNNPPTLE 240

GNYSKPLKEF VEACLNKEPS FRPTAKELLK HKFIIRNAKK TSYLTELIDR YKRWKAEQSH 300

EDSSSEDSDV ETDGQASGGS DSGDWIFTIR EKDPKNLENG TLQLSDLERN KMKDIPKRPF 360

SQCLSTIISP LFAELKEKSQ ACGGNLGSIE ELRGAIYLAE EACPGISDTM VAQLVQRLQR 420

YSLSGGGASA H 431

Nucleotide Sequence
(FASTA)

ATGGCCCACT CCCCGGTGCA GTCGGGCCTG CCGGGCATGC AGAACCTGAA AGCAGACCCA 60

GAAGAGCTTT TTACCAAGCT AGAGAAGATT GGAAAGGGCT CTTTTGGTGA AGTGTTCAAA 120

GGCATTGACA ATCGGACTCA GAAAGTGGTG GCCATAAAAA TCATTGATCT GGAAGAAGCC 180

GAGGACGAGA TAGAGGACAT CCAACAAGAG ATCACAGTGC TGAGCCAGTG TGACAGTCCC 240

TACGTCACCA AGTACTATGG ATCCTATCTC AAGGATACTA AGTTGTGGAT AATCATGGAG 300

TATCTTGGTG GAGGCTCTGC CCTGGATCTG TTAGAGCCTG GCCCTTTAGA TGAAATTCAG 360

ATTGCAACCA TATTACGAGA AATTCTGAAA GGACTTGATT ATCTACACTC GGAGAAGAAA 420

ATTCACAGAG ATATTAAAGC GGCCAATGTT CTGCTCTCTG AACATGGAGA GGTGAAGCTG 480

GCAGACTTTG GAGTGGCCGG CCAGCTGACG GATACCCAGA TCAAAAGGAA CACCTTCGTG 540

GGTACCCCCT TCTGGATGGC GCCGGAGGTC ATCAAGCAGT CAGCCTACGA CTCAAAGGCA 600

GACATCTGGT CCCTTGGCAT CACCGCAATA GAACTGGCCA AAGGAGAGCC ACCACATTCT 660

GAGCTGCACC CCATGAAGGT GTTATTCCTC ATCCCAAAGA ACAACCCTCC CACACTGGAA 720

GGGAACTACA GCAAACCCCT CAAGGAGTTC GTGGAGGCCT GCCTGAACAA GGAGCCCAGC 780

TTTAGGCCCA CTGCTAAGGA ATTATTGAAG CACAAATTCA TAATCCGCAA TGCAAAGAAA 840

ACGTCCTACT TGACCGAGCT TATCGACAGG TACAAGAGGT GGAAGGCGGA GCAGAGCCAC 900

GAGGACTCCA GCTCGGAGGA CTCTGACGTG GAGACAGATG GCCAGGCGTC TGGAGGCAGC 960

GACTCTGGGG ACTGGATCTT CACTATCCGG GAGAAAGATC CCAAGAATCT GGAGAACGGA 1020

ACTCTTCAGC TCTCGGACTT GGAAAGAAAT AAGATGAAAG ATATCCCAAA GAGGCCTTTC 1080

TCTCAGTGTT TATCCACAAT CATTTCTCCT CTGTTTGCGG AGCTGAAAGA GAAGAGCCAG 1140

GCATGCGGAG GGAACTTGGG GTCAATAGAA GAGCTGCGGG GAGCCATCTA CTTGGCGGAA 1200

GAGGCCTGCC CTGGGATCTC AGACACTATG GTGGCACAGC TTGTGCAGCG GCTGCAGAGA 1260

TATTCTCTGA GTGGCGGAGG AGCCTCAGCG CACTGA 1296

Domain Profile

S: 1     yelleeigkGalgkvylarekktgelvaikkinlekq.ekleelqkeisvlkklkhpniv  59

         ++ le+igkG++g+v+++ +++t+++vaik+i+le+  +++e++q+ei+vl+++++p+++

Q: 24    FTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAeDEIEDIQQEITVLSQCDSPYVT  83

         7889*******************************99899********************

S: 60    eylesflkeeelwlvmeylagGslsdlvkeelkselkEeqiayilrevlkaLeylHskkv  119

         +y++s+lk+++lw++meyl gGs+ dl++     +l+E qia+ilre+lk+L+ylHs+k+

Q: 84    KYYGSYLKDTKLWIIMEYLGGGSALDLLE---PGPLDEIQIATILREILKGLDYLHSEKK  140

         *****************************...****************************

S: 120   iHrdikakniLlteegeVkLsdfgvaaqlektrkkrktvvgtpywmAPEviaqeekgYde  179

         iHrdika+n+Ll+e+geVkL+dfgva ql++t+ kr+t+vgtp+wmAPEvi+  ++ Yd+

Q: 141   IHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIK--QSAYDS  198

         ****************************************************..9*****

S: 180   kaDiwslGItaielaegepPfkdleplkallkilkneppklkskkkfskefkefvekclq  239

         kaDiwslGItaiela+gepP+++l+p+k+l++i+kn+pp+l+ +  +sk +kefve+cl+

Q: 199   KADIWSLGITAIELAKGEPPHSELHPMKVLFLIPKNNPPTLEGN--YSKPLKEFVEACLN  256

         *****************************************776..**************

S: 240   knpekRpsasellkhkFl  257

         k+p++Rp+a+ellkhkF+

Q: 257   KEPSFRPTAKELLKHKFI  274

         *****************7

Domain Sequence
(FASTA)

FTKLEKIGKG SFGEVFKGID NRTQKVVAIK IIDLEEAEDE IEDIQQEITV LSQCDSPYVT 60

KYYGSYLKDT KLWIIMEYLG GGSALDLLEP GPLDEIQIAT ILREILKGLD YLHSEKKIHR 120

DIKAANVLLS EHGEVKLADF GVAGQLTDTQ IKRNTFVGTP FWMAPEVIKQ SAYDSKADIW 180

SLGITAIELA KGEPPHSELH PMKVLFLIPK NNPPTLEGNY SKPLKEFVEA CLNKEPSFRP 240

TAKELLKHKF I 251

Keyword

Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006917	; P:induction of apoptosis
GO:0008631	; P:intrinsic apoptotic signaling pathway in response to oxidative stress
GO:0030336	; P:negative regulation of cell migration
GO:0046777	; P:protein autophosphorylation
GO:0048679	; P:regulation of axon regeneration

KEGG

mmu:223255

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.

Prints

Created Date

20-Feb-2013