EKS-MUM-00421

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00421

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	364.3	1.1E-108	689	945	257

Status

Reviewed

Ensembl Protein

ENSMUSP00000093485

UniProt Accession

O35099; Q14AY5;

Protein Name

Mitogen-activated protein kinase kinase kinase 5

Protein Synonyms/Alias

Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5;

Gene Name

Map3k5

Gene Synonyms/Alias

Map3k5; Ask1, Mekk5;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000071369	ENSMUSP00000122553	ENSMUST00000152533
ENSMUSG00000071369	ENSMUSP00000093485	ENSMUST00000095806
ENSMUSG00000071369	ENSMUSP00000114518	ENSMUST00000129437
ENSMUSG00000071369	ENSMUSP00000112864	ENSMUST00000120259

Organism

Mus musculus

Functional Description

Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Protein Length

1380

Protein Sequence
(FASTA)

MGTEAGEGIT FSVPPFASVG FCTIPEGGSC RRGGGAATAA EGEPSLQPLL VPPPPPPPGS 60

FWNVESAAAP GTSCPTTAPG SSATRGRGNS GSGGGRRTTV AYVINEASQG QLVVAESEAL 120

QSLREACEAV GATLETLHFG KLDFGETAVL DRFYNADIAV VEMSDAFRQP SLFYHLGVRE 180

SFSMANNIIL YCDTNSDSLQ SLKEIICQKN TVCTGNYTFI PYMVTPHNKV YCCDSSFMKG 240

LTELMQPNFE LLLGPICLPL VDRFVQLLKV AQASSSQYFR ESILSDIRKA RNLYTGKELA 300

AELARIRQRV DNIEVLTADI VINLLLSYRD IQDYDSIVKL VETLEKLPTF DLASHHHVKF 360

HYAFALNRRN LPGDRAKALD IMIPMVQSEE QVASDMYCLV GRIYKDMFLD SNFTDTESRD 420

HGASWFKKAF ESEPTLQSGI NYAVLLLAAG HQFESSFELR KVGVKLSSLL GKKGNLEKLQ 480

SYWEVGFFLG ASVLANDHLR VIQASEKLFR LKTPAWYLKS IVETILIYKH FVKLTTEQPS 540

AKQELVDFWM DFLVEATKTD VTVVRFPVLI LEPTKIYQPS YLSINNEVEE KTISIWHVLP 600

DDKKGIHEWN FGASSVRGVS ISKFEERCCF LYVLHNSDDF QIYFCTELHC KRFFEMVNTI 660

TEEKGRGAED GDCEGDSLEY DYEYDENGDR VVLGKGTYGI VYAGRDLSNQ VRIAIKEIPE 720

RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL 780

KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN 840

PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE LGEPQAAMFK 900

VGMFKVHPEI PESMSAEAKA FILKCFEPDP DKRACANDLL IDEFLKVSSK KKKTQPKLSA 960

LSTGSNEYLR SISLPVPVLV EDTSSSSEYG SVSPDTELKA DPFSFKARAK SCGEKDGKGI 1020

RTLFLGIPDE NFEDHSAPPS PEEKDSGFFM LRKDSERRAT LHRILTEDQD KVVRNLMESL 1080

AQGAEEPKLK WEHITTLISS LREFVRSTDR KIIATTLSKL KLELDFDSHG ISQVQVVLFG 1140

FQDAVNKVLR NHNIKPHWMF ALDSIIRKAV QTAITILVPE LRPHFSLASE SDTADPEDLD 1200

VEDEHEELSS NQTVRRPQAI TEDAVATSGV STLSSTVSHD SQNAHRSLNV QLGRMKIETN 1260

RLLEELVRKE RELQALLHQA IEEKDQEIRH LKLKSQPIDI PGFPVCHLNS PGTTTEDSEL 1320

PGWLRENGAD EDTISRFLAE DYTLVDVLYY VTRDDLKCLR LRGGMLCTLW KAIIDFRNKC 1380

Nucleotide Sequence
(FASTA)

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 60

TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 120

GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 180

TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 240

AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 300

GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 360

CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 420

AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 480

GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 540

AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 600

TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 660

CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 720

CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 780

GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 840

GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 900

GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 960

GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 1020

GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 1080

CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 1140

ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 1200

GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 1260

CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 1320

AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 1380

AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 1440

AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 1500

GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 1560

ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 1620

GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 1680

GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 1740

TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 1800

GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 1860

ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 1920

CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 1980

ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 2040

GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 2100

GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 2160

AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 2220

CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 2280

ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 2340

AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 2400

CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 2460

TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 2520

CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 2580

GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 2640

ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 2700

GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 2760

TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 2820

ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 2880

CTCTCGACTG GATCGAATGA GTATCTTAGA AGCATCTCCC TGCCGGTCCC TGTCCTGGTA 2940

GAAGACACCA GCAGCAGTAG CGAGTATGGC TCTGTTTCTC CTGATACAGA GCTGAAGGCG 3000

GACCCCTTCT CCTTCAAAGC CAGAGCTAAG TCCTGTGGAG AAAAGGACGG GAAAGGGATA 3060

CGGACATTGT TCCTGGGCAT TCCAGATGAA AATTTTGAAG ATCACAGTGC GCCCCCATCT 3120

CCTGAAGAGA AAGACTCGGG CTTTTTTATG CTGAGAAAGG ATAGTGAGAG GCGAGCTACC 3180

CTTCACAGAA TCCTGACCGA AGACCAGGAC AAGGTTGTAA GGAACCTAAT GGAATCTCTG 3240

GCCCAGGGTG CTGAAGAGCC TAAACTAAAG TGGGAACACA TCACAACCCT CATCTCGAGC 3300

CTCAGAGAGT TTGTGAGGTC CACTGACCGA AAAATCATAG CCACTACACT GTCCAAGCTC 3360

AAGCTGGAGC TGGACTTCGA CAGCCACGGC ATCAGCCAGG TGCAGGTGGT CCTCTTTGGC 3420

TTCCAGGATG CGGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCACA CTGGATGTTT 3480

GCCCTGGACA GCATCATCCG AAAGGCTGTG CAGACTGCAA TTACCATTCT GGTTCCAGAG 3540

TTGAGGCCAC ATTTTAGCCT TGCATCAGAG AGCGACACAG CTGATCCGGA AGACCTGGAT 3600

GTCGAAGATG AACACGAGGA ATTGTCTTCA AATCAGACAG TCCGACGGCC TCAGGCCATC 3660

ACTGAGGACG CTGTGGCTAC CTCGGGGGTG AGCACGCTAA GCTCCACTGT GTCCCATGAC 3720

TCCCAGAATG CACACCGGTC GCTGAACGTG CAGCTTGGAA GGATGAAGAT TGAAACTAAT 3780

AGGTTACTTG AAGAGCTGGT TCGGAAAGAG AGAGAGTTAC AGGCACTCCT CCATCAAGCC 3840

ATTGAGGAAA AAGACCAAGA AATTAGGCAC CTGAAGCTCA AGTCCCAGCC CATAGATATC 3900

CCTGGGTTTC CTGTGTGCCA CCTGAACTCT CCTGGCACGA CCACGGAAGA TTCTGAACTT 3960

CCTGGCTGGC TGAGAGAAAA TGGAGCTGAT GAAGACACTA TAAGTCGGTT TCTGGCTGAG 4020

GATTACACGC TAGTGGATGT TCTTTACTAC GTCACACGTG ATGATCTGAA GTGCCTCAGA 4080

CTAAGGGGCG GGATGCTGTG CACGCTGTGG AAGGCCATCA TTGACTTTCG GAACAAATGC 4140

TGA 4143

Domain Profile

S: 3     krevigkGsfgsvyaaldvdtgellavKeveikpekksekkevkalkeEikllkelkHen  62

         +r v+gkG++g vya++d++++ ++a+Ke+   pe++s+  ++++l+eEi+l+k+lkH+n

Q: 689   DRVVLGKGTYGIVYAGRDLSNQVRIAIKEI---PERDSR--YSQPLHEEIALHKHLKHKN  743

         5789**************************...888775..9******************

S: 63    ivkyyGveveeeelkiflEyvegGslsslle.kygkle..esvikkytkqileglayLHe  119

         iv+y+G+ +e++ +kif+E+v+gGsls+ll+ k+g+l+  e++i +ytkqilegl+yLH+

Q: 744   IVQYLGSFSENGFIKIFMEQVPGGSLSALLRsKWGPLKdnEQTIGFYTKQILEGLKYLHD  803

         *******************************9*****9999*******************

S: 120   kkivHRDiKgaNillds.sgvvklsDFGaskrlaelntkaaelesltGtlaymaPEvikk  178

         ++ivHRDiKg+N+l+++ sgv+k+sDFG+skrla +n++   +e++tGtl+ymaPE+i+k

Q: 804   NQIVHRDIKGDNVLINTySGVLKISDFGTSKRLAGINPC---TETFTGTLQYMAPEIIDK  860

         ***************************************...8*****************

S: 179   kekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklseegkd  238

         + +gyg++aDiWslgc++iem+tgk+Pf el+e++a+mfkvg+ k++Peipe++s+e+k+

Q: 861   GPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKA  920

         ************************************************************

S: 239   flekcleldpkkrpsaeeLLkhefl  263

         f+ kc+e dp+kr++a++LL +efl

Q: 921   FILKCFEPDPDKRACANDLLIDEFL  945

         *********************9996

Domain Sequence
(FASTA)

DRVVLGKGTY GIVYAGRDLS NQVRIAIKEI PERDSRYSQP LHEEIALHKH LKHKNIVQYL 60

GSFSENGFIK IFMEQVPGGS LSALLRSKWG PLKDNEQTIG FYTKQILEGL KYLHDNQIVH 120

RDIKGDNVLI NTYSGVLKIS DFGTSKRLAG INPCTETFTG TLQYMAPEII DKGPRGYGKA 180

ADIWSLGCTI IEMATGKPPF YELGEPQAAM FKVGMFKVHP EIPESMSAEA KAFILKCFEP 240

DPDKRACAND LLIDEFL 257

Keyword

Apoptosis; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Endoplasmic reticulum; Immunity; Innate immunity; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005783	; C:endoplasmic reticulum
GO:0005524	; F:ATP binding
GO:0008656	; F:cysteine-type endopeptidase activator activity involved in apoptotic process
GO:0000287	; F:magnesium ion binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0042803	; F:protein homodimerization activity
GO:0000187	; P:activation of MAPK activity
GO:0070301	; P:cellular response to hydrogen peroxide
GO:0010421	; P:hydrogen peroxide-mediated programmed cell death
GO:0045087	; P:innate immune response
GO:0008631	; P:intrinsic apoptotic signaling pathway in response to oxidative stress
GO:0007254	; P:JNK cascade
GO:0043065	; P:positive regulation of apoptotic process

KEGG

mmu:26408

;

InterPros

IPR025136	; DUF4071.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR013761	; SAM/pointed.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF13281	; DUF4071; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013