EKS-MUM-00217

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00217

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NKF3	577.1	2.9E-173	1318	1652	335

Status

Reviewed

Ensembl Protein

ENSMUSP00000109901

UniProt Accession

Q69Z38; E9QKY2; Q8BX56; Q8R365;

Protein Name

Pseudopodium-enriched atypical kinase 1

Protein Synonyms/Alias

Sugen kinase 269; Tyrosine-protein kinase SgK269;

Gene Name

Peak1

Gene Synonyms/Alias

Peak1; Kiaa2002, Sgk269;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000074305	ENSMUSP00000109901	ENSMUST00000061552

Organism

Mus musculus

Functional Description

Tyrosine kinase that may play a role in cell spreadingand migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN (By similarity).

Protein Length

1735

Protein Sequence
(FASTA)

MSACNTFTEH VWKPGECKNC FKPKSLHQLP PDSEKTPITH GSGKTNANHS NNHRVRSTGN 60

FRPPVAKKPT IAVKPTMMVA DGQSVCGELS IQEHCENKPV ILGWNQNKTS LSQKPLNNNS 120

EGDAEGFGSD PQQCANNDSA QKISNNNNGL TEVLKEIAGL EATPPVRGNE TNARETFLGR 180

INDCYKRSLE RKIPPSCMTG SMKDSQGKHV ILSGSAEVIS NEGGRFCYPE FSSGEESEED 240

VLFSNMEEEH ESWDESDEEL LAMEIRMRGQ PRFANFRANT LSPVRFFVSK KWNTIPLRNK 300

SLQRICAVDY DDSYDEILNG YEENSGVSYG QGSVQSTISS DCTSPGSSFT EESRSETASS 360

LSQKVCNGGI SPGNPGNSKD IAETESNFES PPGNNEEKDE SLTSKSSVKV PETHKAVLAL 420

RLQEKDGKIA VHTEKPESKA STDIAGQAVT ISLVPVEEQT KPYRVVNLEQ PLCKPYTVVD 480

VSAAMASEHL GRPKIKGSSS TPNSPVTSPA LTPGQINAHL KKSSAIRYQE VWTSSTSPRQ 540

KIPKIELSTG GPGPNVPPRK NCHKSAPTSP TATNISSKTI PVKSPNLSEI KFNSYNNAGM 600

PPFPIIIHDE PSYARSSKNA IKVPIVINPN AYDNLAIYKS FLGTSGELSV KEKTTSVISH 660

TYEEIETESK VSDSTPSKLT DCPQAKGFSN STERKRGSVA QKVQEFNNCL NRGQSSPQRS 720

YSSTHSSPAK IQRPTQEPAG KTEGAQGSQV PGSSSNSTRE KASAVLCQIV ASIQPPQTPP 780

EAPQSSPKAC SVEELYAVPP DADTTKSIPK NPPVRPKSLF TSQSSGEGEA HQTTESPTAK 840

IQKDPSTKPV TSPPSKLVTS AQSEPPPPFP PPRSTSSPYH ASNLLQRHFT NWTKPTSPTR 900

STEAESILHS EGSRRAADAK PKRWISFKSF FRRRKTDEEE EKEKEREKGK LVGLDGTVIH 960

MLPPPPVQRH HWFTEAKGEA SEKPAIVFMY RCDPDQGHLS VDQSKAGAEK GRAEEVLLRN 1020

SEEKKSSYLP SQIPDKACSR VTHEVAGELS PRDPRTPAGK QDGTSVTPTL PPPDLEREEE 1080

KDDTLDPTDV SPCSATYSNL GQSRAAMIPP KHPRHPKGAV DDAIAFGEKT DQEGLNASQP 1140

TPPPLPKKMI RANTEPISKD LQKAMESSLC VMANPTYDID PNWDASSAGS SISYELKGLD 1200

VESYESLERP LHKERPVPSA ANSISSLATL SVKDRFSNSM ESLSSRRGLS YRQTRSIQKP 1260

QRQALYRGLD NREEVVGKLR SLHTDALKRL AVKCEDLFMA GQKDQLRFGV DSWSDFRLTS 1320

DKPCCEAGDA VYYTASYAKD PLSNYAVKIC KSKAKESQQY YHSLAVRQSL PVHFNIQQDC 1380

GHFLAEVPSR LLPWEDPDAP EKAEDGTEDS EEEGKAETLG GNPEPCSETE PSQKENQRVT 1440

NRKQRSHVVV ITREVPHLTV ADFVRDSLAH HGNSPDLYER QVCLLLLQLC SGLEHLKPYH 1500

VTHCDLRLEN LLLVQHQPGG AAQGPSPADP CPTLACPTRL IVSNFSQAKQ KSHLVDPQIL 1560

RDQSRLAPEI ITATQYKKCD EFQTGILIYE MLHLPNPFDE NPELKEKEYT RTDLPRIPLR 1620

SPYSWGLQQL ASCLLNPNPS ERILISDAKG ILQCLLWGPR EDLFQIFTTS ATLAQKNALL 1680

QNWLDIKRTL LMIKFAEKSL DREGGISLED WLCAQYLAFA TTDSLSYIVK ILQYR 1735

Nucleotide Sequence
(FASTA)

ATGTCTGCTT GTAACACCTT TACTGAACAC GTTTGGAAAC CTGGCGAATG CAAGAATTGC 60

TTTAAACCTA AAAGCTTGCA CCAGCTTCCT CCAGACTCTG AGAAGACGCC CATCACCCAC 120

GGCAGTGGGA AAACTAATGC CAATCACAGT AACAACCACC GAGTCAGGAG CACGGGCAAC 180

TTCCGGCCCC CTGTGGCTAA AAAGCCCACC ATCGCTGTGA AGCCCACTAT GATGGTGGCA 240

GATGGGCAAA GCGTGTGTGG CGAGCTAAGC ATCCAGGAAC ACTGTGAGAA CAAGCCTGTC 300

ATCCTGGGGT GGAACCAAAA CAAGACGTCC CTGAGCCAGA AACCACTTAA CAACAATTCT 360

GAAGGTGATG CTGAAGGCTT TGGCAGTGAT CCTCAGCAGT GTGCCAATAA TGACAGTGCA 420

CAGAAGATAT CCAATAACAA CAACGGGCTA ACTGAAGTGC TAAAGGAGAT AGCGGGCCTG 480

GAAGCCACCC CTCCGGTCAG AGGAAATGAA ACAAACGCCA GAGAAACATT CTTGGGAAGA 540

ATAAATGATT GCTACAAACG ATCGTTGGAA AGAAAGATCC CACCCAGTTG CATGACCGGG 600

AGCATGAAGG ACTCTCAGGG CAAACACGTC ATCCTGAGCG GGAGCGCCGA GGTCATCAGT 660

AACGAAGGGG GCAGGTTCTG CTACCCTGAG TTCTCCAGTG GTGAGGAGAG TGAGGAAGAT 720

GTGCTTTTCA GTAACATGGA GGAGGAGCAT GAGAGCTGGG ATGAGAGCGA TGAAGAGCTG 780

TTGGCCATGG AGATTCGCAT GAGAGGGCAG CCTCGCTTTG CTAACTTCAG GGCAAACACC 840

CTGTCTCCTG TTCGATTCTT TGTGAGCAAA AAGTGGAATA CTATCCCCTT ACGGAATAAG 900

TCTCTGCAGA GAATCTGTGC TGTGGACTAT GACGACAGCT ATGATGAAAT CCTGAATGGT 960

TATGAGGAAA ATTCTGGGGT CTCCTATGGT CAAGGAAGCG TTCAGAGCAC AATATCATCT 1020

GACTGCACAT CACCAGGCTC CTCCTTCACA GAAGAGTCAC GCTCTGAAAC AGCCAGCAGT 1080

TTATCCCAGA AGGTCTGTAA TGGGGGGATA TCTCCTGGTA ACCCAGGAAA TTCTAAGGAC 1140

ATAGCAGAAA CTGAGTCTAA TTTTGAAAGT CCTCCTGGTA ACAATGAGGA GAAGGACGAA 1200

TCACTGACTT CCAAAAGCTC GGTGAAAGTT CCAGAGACGC ACAAAGCTGT CCTTGCTCTC 1260

CGTTTACAGG AGAAGGATGG CAAGATTGCA GTACATACTG AGAAGCCAGA GAGCAAAGCT 1320

TCTACAGATA TCGCTGGCCA GGCAGTCACC ATAAGCCTCG TCCCAGTAGA AGAACAGACA 1380

AAGCCCTACC GTGTGGTAAA TCTGGAACAG CCGCTGTGTA AGCCATATAC CGTTGTGGAT 1440

GTGTCGGCAG CCATGGCCAG CGAGCACCTG GGGCGACCCA AGATAAAAGG TTCATCTTCT 1500

ACTCCAAACT CTCCAGTTAC ATCACCTGCA TTGACACCAG GACAAATAAA TGCCCATCTC 1560

AAAAAATCCA GTGCAATCCG ATACCAGGAA GTGTGGACGT CCAGCACCAG TCCACGACAA 1620

AAGATCCCTA AAATAGAATT AAGTACTGGG GGACCTGGGC CAAATGTCCC TCCAAGGAAA 1680

AACTGCCATA AGTCGGCGCC CACTTCACCC ACGGCTACAA ACATCTCCTC TAAAACCATC 1740

CCTGTTAAGT CACCTAATTT GTCTGAAATA AAATTTAATA GTTACAACAA TGCCGGGATG 1800

CCACCTTTTC CCATTATCAT CCATGATGAG CCATCCTATG CCCGGAGTTC CAAAAATGCC 1860

ATTAAAGTCC CCATTGTTAT TAATCCAAAT GCGTATGACA ATCTCGCCAT CTACAAAAGT 1920

TTTCTGGGAA CAAGTGGAGA ACTCTCTGTG AAGGAAAAAA CCACAAGCGT AATAAGCCAT 1980

ACTTACGAAG AAATAGAAAC TGAAAGCAAA GTGTCTGATA GCACCCCCAG CAAGCTCACA 2040

GACTGTCCCC AAGCTAAAGG GTTTTCCAAC AGCACAGAGC GGAAAAGAGG CTCAGTAGCG 2100

CAGAAGGTGC AGGAGTTTAA CAATTGTCTC AACAGAGGTC AGTCTTCTCC ACAAAGAAGC 2160

TACAGTTCCA CCCACAGCTC CCCAGCAAAA ATCCAGAGAC CCACTCAAGA GCCTGCGGGC 2220

AAAACGGAAG GGGCACAAGG GTCTCAGGTG CCAGGCAGCA GCAGCAACAG CACTAGGGAG 2280

AAAGCAAGCG CCGTCCTATG TCAGATTGTG GCATCTATCC AACCCCCCCA GACTCCTCCA 2340

GAAGCACCTC AATCTAGCCC TAAGGCTTGC AGTGTGGAAG AGCTTTATGC CGTTCCCCCA 2400

GATGCAGATA CTACAAAAAG TATTCCTAAA AACCCACCAG TCAGACCCAA ATCTCTTTTT 2460

ACATCTCAGT CTAGTGGGGA AGGTGAGGCC CATCAGACCA CAGAAAGCCC TACTGCCAAA 2520

ATTCAGAAAG ATCCATCCAC GAAGCCAGTG ACTTCTCCTC CCTCCAAATT AGTGACGAGC 2580

GCCCAGAGTG AGCCACCGCC TCCCTTTCCC CCACCACGGT CTACTTCCTC CCCTTACCAT 2640

GCAAGTAACC TGTTGCAAAG GCATTTTACC AATTGGACGA AGCCAACCAG TCCTACCCGA 2700

TCAACAGAAG CTGAATCAAT TTTGCACTCT GAAGGCAGCA GGCGAGCCGC TGATGCAAAA 2760

CCTAAACGCT GGATATCATT TAAAAGCTTC TTCCGCCGCC GCAAAACAGA TGAGGAAGAA 2820

GAGAAGGAGA AAGAGCGAGA GAAAGGGAAG CTGGTGGGCC TGGACGGGAC AGTCATCCAC 2880

ATGCTGCCGC CCCCTCCAGT CCAGCGCCAT CACTGGTTCA CAGAGGCCAA AGGAGAGGCC 2940

AGCGAGAAGC CTGCTATAGT CTTCATGTAC AGGTGTGACC CTGACCAAGG CCACCTCAGT 3000

GTGGACCAAA GCAAGGCTGG GGCAGAGAAA GGAAGAGCAG AGGAGGTGTT ACTGCGGAAC 3060

TCGGAAGAGA AGAAAAGTAG CTATCTTCCA TCACAGATTC CTGACAAAGC TTGCAGTCGT 3120

GTGACCCATG AAGTGGCAGG AGAACTTTCT CCTCGGGATC CAAGAACTCC TGCTGGGAAG 3180

CAAGATGGCA CTTCAGTCAC ACCAACACTG CCTCCACCTG ACCTGGAAAG GGAAGAAGAA 3240

AAAGATGACA CTTTGGATCC TACTGACGTG AGTCCCTGCA GTGCAACCTA CAGCAACCTA 3300

GGACAATCTC GAGCAGCTAT GATACCTCCC AAGCATCCAC GGCATCCCAA GGGAGCGGTG 3360

GATGATGCCA TTGCCTTTGG AGAGAAGACA GACCAAGAAG GACTGAATGC TTCCCAACCC 3420

ACACCACCCC CACTGCCAAA GAAAATGATA CGAGCCAATA CCGAGCCCAT CTCCAAAGAC 3480

CTTCAGAAAG CCATGGAAAG CAGTCTTTGC GTCATGGCTA ATCCCACCTA CGATATTGAT 3540

CCCAACTGGG ATGCCAGCAG TGCTGGGTCT TCTATTAGCT ATGAACTCAA GGGACTAGAC 3600

GTTGAGTCTT ATGAATCCTT GGAGAGACCT TTGCATAAGG AGAGACCCGT TCCTTCAGCA 3660

GCAAACAGTA TCTCCAGCCT AGCCACTCTC AGTGTTAAGG ACAGATTTTC TAACAGTATG 3720

GAGTCACTCT CCAGTCGACG TGGTCTCTCC TATAGACAGA CTCGAAGCAT CCAGAAGCCA 3780

CAGAGACAAG CACTTTATCG AGGACTTGAC AATCGGGAGG AAGTGGTGGG TAAACTCCGA 3840

AGCCTTCATA CAGATGCCTT GAAGAGACTG GCAGTTAAAT GTGAAGACCT CTTCATGGCT 3900

GGGCAGAAAG ACCAACTCCG CTTTGGAGTA GACAGCTGGT CAGACTTCAG GCTAACCAGC 3960

GACAAACCGT GCTGCGAAGC TGGAGATGCG GTTTACTACA CTGCCTCGTA TGCAAAAGAT 4020

CCACTCAGTA ACTATGCAGT CAAGATCTGC AAGAGCAAAG CTAAGGAGTC TCAGCAGTAT 4080

TACCATAGCC TGGCTGTCCG GCAGAGTCTG CCTGTGCATT TTAACATCCA GCAGGATTGT 4140

GGCCATTTCC TTGCTGAAGT CCCTAGTCGT CTGCTTCCCT GGGAGGATCC TGATGCTCCT 4200

GAAAAGGCAG AGGATGGCAC TGAAGACAGC GAAGAAGAGG GGAAGGCAGA AACTTTGGGG 4260

GGAAATCCTG AGCCCTGCTC TGAAACAGAG CCATCCCAGA AAGAGAACCA ACGAGTCACA 4320

AACAGGAAAC AAAGAAGCCA CGTTGTGGTC ATCACCAGGG AGGTTCCCCA TCTCACTGTG 4380

GCCGACTTCG TGCGAGATTC TTTGGCCCAT CATGGGAACA GCCCTGACTT GTATGAGAGG 4440

CAAGTGTGTC TGCTGCTCTT ACAGCTGTGC TCTGGGCTTG AGCACCTCAA GCCCTACCAC 4500

GTCACGCACT GCGATCTTCG CCTGGAGAAC TTGCTGCTTG TCCAGCACCA ACCTGGGGGA 4560

GCTGCCCAGG GCCCTTCTCC TGCAGACCCT TGCCCCACCT TAGCTTGTCC TACAAGGCTC 4620

ATTGTGAGTA ACTTCTCTCA GGCCAAGCAG AAGAGCCACC TGGTGGACCC CCAGATCCTC 4680

CGAGACCAGT CCCGCCTTGC CCCAGAGATC ATCACGGCCA CCCAGTATAA GAAGTGTGAT 4740

GAATTCCAGA CGGGCATTCT CATCTATGAG ATGCTGCACC TGCCCAACCC TTTTGACGAG 4800

AACCCGGAGC TGAAGGAGAA GGAGTACACG CGCACAGATC TGCCTCGGAT CCCTCTCCGC 4860

TCCCCGTACT CCTGGGGTCT ACAGCAGCTT GCTAGCTGCC TCCTGAATCC CAACCCTTCT 4920

GAGCGCATCC TCATTTCTGA CGCCAAAGGC ATCCTCCAAT GTCTGCTCTG GGGCCCCCGA 4980

GAAGATCTCT TTCAAATCTT CACCACCTCT GCAACCCTCG CACAGAAGAA CGCTCTGCTC 5040

CAAAACTGGT TAGACATCAA GCGAACCCTG CTGATGATCA AGTTCGCGGA GAAGTCCCTG 5100

GACAGAGAGG GGGGCATCAG CCTGGAGGAC TGGCTTTGTG CTCAGTATCT GGCCTTTGCC 5160

ACTACAGACT CCCTGAGCTA TATTGTAAAA ATCCTGCAAT ACCGTTAA 5208

Domain Profile

S: 1     ltsdkpcceagdavyytasyakdplsnyavkickskekksqsy.....vrqslpvhfniq  55

         ltsdkpcceagdavyytasyakdplsnyavkicksk+k+sq+y     vrqslpvhfniq

Q: 1318  LTSDKPCCEAGDAVYYTASYAKDPLSNYAVKICKSKAKESQQYyhslaVRQSLPVHFNIQ  1377

         79**********************************************************

S: 56    qdcGhflaevpsrlla..dpdapekd...................pkpasetepsake..  92

         qdcGhflaevpsrll+  dpdapek+                   p+p+seteps+ke

Q: 1378  QDCGHFLAEVPSRLLPweDPDAPEKAedgtedseeegkaetlggnPEPCSETEPSQKEnq  1437

         *************************99*********************************

S: 93    .......rdhvvvitrevphltvadfvrdslashraepelyerrvcllllqlcsGlehlk  145

                r+hvvvitrevphltvadfvrdsla+h+++p+lyer+vcllllqlcsGlehlk

Q: 1438  rvtnrkqRSHVVVITREVPHLTVADFVRDSLAHHGNSPDLYERQVCLLLLQLCSGLEHLK  1497

         ************************************************************

S: 146   eygvihrdlrlenlllvhlqpqagaaqaaapaepaptsaapsaapaattasqegprekql  205

         +y+v+h+dlrlenlllv++qp +gaaq+++pa+p+pt+a+p

Q: 1498  PYHVTHCDLRLENLLLVQHQP-GGAAQGPSPADPCPTLACP-------------------  1537

         *********************.*******************...................

S: 206   trlivsnflkakqksgglvdlqikkdqarlapeivsasqykkfdefqtGiliyellhlpn  265

         trlivsnf++akqks +lvd+qi++dq+rlapei++a+qykk+defqtGiliye+lhlpn

Q: 1538  TRLIVSNFSQAKQKS-HLVDPQILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPN  1596

         9**************.********************************************

S: 266   pfevraelkereyrredlprlpllslysrGlqqlasllleadpikriliseakrvl  321

         pf++++elke+ey+r+dlpr+pl+s+ys+Glqqlas+ll+++p++rilis+ak++l

Q: 1597  PFDENPELKEKEYTRTDLPRIPLRSPYSWGLQQLASCLLNPNPSERILISDAKGIL  1652

         ******************************************************98

Domain Sequence
(FASTA)

LTSDKPCCEA GDAVYYTASY AKDPLSNYAV KICKSKAKES QQYYHSLAVR QSLPVHFNIQ 60

QDCGHFLAEV PSRLLPWEDP DAPEKAEDGT EDSEEEGKAE TLGGNPEPCS ETEPSQKENQ 120

RVTNRKQRSH VVVITREVPH LTVADFVRDS LAHHGNSPDL YERQVCLLLL QLCSGLEHLK 180

PYHVTHCDLR LENLLLVQHQ PGGAAQGPSP ADPCPTLACP TRLIVSNFSQ AKQKSHLVDP 240

QILRDQSRLA PEIITATQYK KCDEFQTGIL IYEMLHLPNP FDENPELKEK EYTRTDLPRI 300

PLRSPYSWGL QQLASCLLNP NPSERILISD AKGIL 335

Keyword

ATP-binding; Cell junction; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0015629	; C:actin cytoskeleton
GO:0005737	; C:cytoplasm
GO:0005925	; C:focal adhesion
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0016477	; P:cell migration
GO:0046777	; P:protein autophosphorylation
GO:0034446	; P:substrate adhesion-dependent cell spreading

KEGG

mmu:244895

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.

Prints

Created Date

20-Feb-2013