EPS-ORC-00020

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-ORC-00020

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	292.9	2.9E-87	31	297	267

Status

Reviewed

Ensembl Protein

ENSOCUP00000008575

UniProt Accession

P35814;

Protein Name

Protein phosphatase 1A

Protein Synonyms/Alias

Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA;

Gene Name

PPM1A

Gene Synonyms/Alias

PPM1A; PPPM1A;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSOCUG00000009955	ENSOCUP00000008575	ENSOCUT00000009955

Organism

Oryctolagus cuniculus

Functional Description

Enzyme with a broad specificity. Negatively regulatesTGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity).

Protein Length

390

Protein Sequence
(FASTA)

AELEDQDIMG AFLDKPKMEK HNAQGQGNGL RYGLSSMQGW RVEMEDAHTA VIGLPSGLET 60

WSFFAVYDGH AGSQVAKYCC EHLLDHITNN QDFKGSAGAP SVENVKNGIR TGFLEIDEHM 120

RVMSEKKHGA DRSGSTAVGV LISPQHTYFI NCGDSRGLLC RNRKVHFFTQ DHKPSNPLEK 180

ERIQNAGGSV MIQRVNGSLA VSRALGDFDY KCVHGKGPTE QLVSPEPEVH DIERSEEDDQ 240

FIILACDGIW DVMGNEELCD FVRSRLEVTD DLEKVCNEVV DTCLYKGSRD NMSVILICFP 300

NAPKVSPEAV KKEAELDKYL ECRVEEILKK QGEGVPDLVH VMRTLASENI PSLPPGGELA 360

SKRNVIEAVY NRLNPYKNDD TDSTSTDDMW 390

Nucleotide Sequence
(FASTA)

GCAGAACTAG AGGATCAAGA CATAATGGGA GCATTTTTAG ACAAGCCAAA GATGGAAAAG 60

CATAATGCCC AGGGGCAGGG TAATGGGTTG CGATATGGGC TAAGCAGCAT GCAAGGCTGG 120

CGCGTGGAAA TGGAGGATGC ACACACGGCT GTGATCGGTT TGCCAAGTGG ACTTGAAACA 180

TGGTCATTCT TTGCTGTGTA TGATGGGCAT GCTGGTTCTC AGGTTGCCAA GTACTGCTGT 240

GAGCATTTGT TAGATCACAT CACCAATAAC CAGGACTTTA AAGGGTCTGC AGGAGCACCG 300

TCTGTGGAAA ACGTCAAGAA TGGAATCAGA ACAGGTTTTC TGGAGATTGA TGAACACATG 360

CGAGTTATGT CCGAGAAGAA GCACGGTGCA GATAGGAGTG GGTCAACAGC GGTGGGGGTC 420

TTAATTTCAC CCCAGCACAC TTACTTCATT AACTGTGGAG ACTCAAGAGG TTTACTTTGT 480

AGGAACAGGA AAGTTCACTT CTTCACACAA GATCACAAAC CAAGTAATCC GCTGGAAAAA 540

GAACGAATTC AGAATGCAGG TGGCTCTGTC ATGATTCAGC GTGTGAATGG CTCTCTGGCT 600

GTATCGAGGG CTCTTGGGGA TTTTGATTAC AAGTGTGTCC ATGGAAAAGG TCCTACAGAG 660

CAGCTTGTCT CTCCAGAGCC TGAAGTCCAT GACATTGAAA GATCTGAAGA AGATGATCAG 720

TTCATTATCC TTGCATGTGA TGGTATCTGG GATGTCATGG GAAACGAAGA GCTCTGTGAT 780

TTTGTAAGAT CCAGACTTGA AGTCACTGAT GACCTTGAGA AAGTTTGCAA TGAAGTAGTC 840

GACACCTGTT TGTATAAGGG AAGTCGAGAC AACATGAGTG TGATTTTGAT CTGTTTTCCA 900

AATGCACCCA AAGTGTCACC AGAAGCGGTG AAGAAGGAGG CAGAGTTGGA CAAGTACCTG 960

GAATGCAGAG TAGAAGAAAT CCTAAAGAAG CAGGGGGAAG GCGTCCCGGA CTTAGTCCAC 1020

GTGATGCGCA CATTAGCCAG CGAGAACATC CCCAGCCTCC CTCCAGGGGG CGAACTGGCC 1080

AGCAAGCGGA ATGTAATTGA AGCCGTTTAC AATAGACTGA ATCCTTACAA AAATGATGAC 1140

ACTGACTCTA CATCAACTGA TGATATGTGG TAA 1173

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g++s++g+r emeda+t++ ++ +     + ++ff+vyDGh+gs+vak+c e+l +++

Q: 31    RYGLSSMQGWRVEMEDAHTAVIGLPSGL---ETWSFFAVYDGHAGSQVAKYCCEHLLDHI  87

         69****************9855554444...45***************************

S: 62    aeele....sseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdskl  117

         +++ +    +++++ ++vk+ ++++Fl++de+++v++ek++  d+sGstav +li++++

Q: 88    TNNQDfkgsAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHT  147

         ***9999999999***********************************************

S: 118   yvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLavsRa  177

         y++n+GdSr +Lcrn+k++  ++dHkps++ E+eRi++aggsv+ +   Rv+G+LavsRa

Q: 148   YFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQ---RVNGSLAVSRA  204

         **********************************************...***********

S: 178   lGdlylk.........pyvtpePevtshrltsede.fliLasDGlwdvvsneeavslvrd  227

         lGd+++k         ++v+pePev+ +++++ed+ f+iLa+DG+wdv+ nee++++vr+

Q: 205   LGDFDYKcvhgkgpteQLVSPEPEVHDIERSEEDDqFIILACDGIWDVMGNEELCDFVRS  264

         ******************************999999************************

S: 228   vlekkeaeqeaakkLvklalargskdnvsaivv  260

         +le +++++++++++v+++l +gs+dn+s+i++

Q: 265   RLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILI  297

         ****************************99976

Domain Sequence
(FASTA)

RYGLSSMQGW RVEMEDAHTA VIGLPSGLET WSFFAVYDGH AGSQVAKYCC EHLLDHITNN 60

QDFKGSAGAP SVENVKNGIR TGFLEIDEHM RVMSEKKHGA DRSGSTAVGV LISPQHTYFI 120

NCGDSRGLLC RNRKVHFFTQ DHKPSNPLEK ERIQNAGGSV MIQRVNGSLA VSRALGDFDY 180

KCVHGKGPTE QLVSPEPEVH DIERSEEDDQ FIILACDGIW DVMGNEELCD FVRSRLEVTD 240

DLEKVCNEVV DTCLYKGSRD NMSVILI 267

Keyword

Complete proteome; Hydrolase; Lipoprotein; Magnesium; Manganese; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0000287	; F:magnesium ion binding
GO:0030145	; F:manganese ion binding
GO:0004721	; F:phosphoprotein phosphatase activity
GO:0070412	; F:R-SMAD binding
GO:0004871	; F:signal transducer activity
GO:0010991	; P:negative regulation of SMAD protein complex assembly
GO:0030512	; P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045893	; P:positive regulation of transcription, DNA-dependent
GO:0030177	; P:positive regulation of Wnt receptor signaling pathway
GO:0006470	; P:protein dephosphorylation
GO:0016055	; P:Wnt receptor signaling pathway

KEGG

InterPros

IPR001932	; PP2C-like.
IPR012911	; PP2C_C.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481	; PP2C; 1.
PF07830	; PP2C_C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013