EKS-PAT-00046

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-PAT-00046

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/RAF	413.0	6.0E-124	458	714	257

Status

Unreviewed

Ensembl Protein

ENSPTRP00000033866

UniProt Accession

H2QVI0;

Protein Name

Protein Synonyms/Alias

V-raf murine sarcoma viral oncogene homolog B1;

Gene Name

ENSG00000157764

Gene Synonyms/Alias

ENSG00000157764; BRAF;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSPTRG00000019769	ENSPTRP00000033866	ENSPTRT00000036626

Organism

Pan troglodytes

Functional Description

Protein Length

766

Protein Sequence
(FASTA)

MAALSGGGGG GAEPGQALFN GDMEPEAGAG AGAAASSAAD PAIPEEVWNI KQMIKLTQEH 60

IEALLDKFGG EHNPPSIYLE AYEEYTSKLD ALQQREQQLL ESLGNGTDFS VSSSASMDTV 120

TSSSSSSLSV LPSSLSVFQN PTDVARSNPK SPQKPIVRVF LPNKQRTVVP ARCGVTVRDS 180

LKKALMMRGL IPECCAVYRI QDGEKKPIGW DTDISWLTGE ELHVEVLENV PLTTHNFVRK 240

TFFTLAFCDF CRKLLFQGFR CQTCGYKFHQ RCSTEVPLMC VNYDQLDLLF VSKFFEHHPI 300

PQEEASLAET ALTSGSSPSA PASDSIGPQI LTSPSPSKSI PIPQPFRPAD EDHRNQFGQR 360

DRSSSAPNVH INTIEPVNID DLIRDQGFRG DGGSTTGLSA TPPASLPGSL TNVKALQKSP 420

GPQRERKSSS SSEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV 480

AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH 540

LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV 600

KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN 660

NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS 720

LPKIHRSASE PSLNRAGFQT EDFSLYACAS PKTPIQAGGY GAFPVH 766

Nucleotide Sequence
(FASTA)

ATGGCGGCGC TGAGCGGTGG CGGTGGTGGC GGCGCGGAGC CGGGCCAGGC TCTGTTCAAC 60

GGGGACATGG AGCCCGAGGC CGGCGCCGGC GCCGGCGCCG CGGCCTCTTC GGCTGCGGAC 120

CCTGCCATTC CGGAGGAGGT GTGGAATATC AAACAAATGA TTAAGTTGAC ACAGGAACAT 180

ATAGAGGCCC TATTGGACAA ATTTGGTGGG GAGCATAATC CACCATCAAT ATATCTGGAG 240

GCCTATGAAG AATACACCAG CAAGCTAGAT GCACTCCAAC AAAGAGAACA ACAGTTATTG 300

GAATCTCTGG GGAACGGAAC TGATTTTTCT GTTTCTAGCT CTGCATCAAT GGATACCGTT 360

ACATCTTCTT CCTCTTCTAG CCTTTCAGTG CTACCTTCAT CTCTTTCAGT TTTTCAAAAT 420

CCCACAGATG TGGCACGGAG CAACCCCAAG TCACCACAAA AACCTATCGT TAGAGTCTTC 480

CTGCCCAACA AACAGAGGAC AGTGGTACCT GCAAGGTGTG GAGTTACAGT CCGAGACAGT 540

CTAAAGAAAG CACTGATGAT GAGAGGTCTA ATCCCAGAGT GCTGTGCTGT TTACAGAATT 600

CAGGATGGAG AGAAGAAACC AATTGGCTGG GACACTGATA TTTCCTGGCT TACTGGAGAA 660

GAATTGCATG TGGAAGTGTT GGAGAATGTT CCACTTACAA CACACAACTT TGTACGAAAA 720

ACGTTTTTCA CCTTAGCATT TTGTGACTTT TGTCGAAAGC TGCTTTTCCA GGGTTTCCGC 780

TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA CAGAAGTTCC ACTGATGTGT 840

GTTAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT TCTTTGAACA CCACCCAATA 900

CCACAGGAAG AGGCGTCCTT AGCAGAGACT GCCCTAACAT CTGGATCATC CCCTTCCGCA 960

CCCGCCTCGG ACTCTATTGG GCCCCAAATT CTCACCAGTC CGTCTCCTTC AAAATCCATT 1020

CCAATTCCAC AGCCCTTCCG ACCAGCAGAT GAAGATCATC GAAATCAATT TGGGCAACGA 1080

GACCGATCCT CATCAGCTCC CAATGTGCAT ATAAACACAA TAGAACCTGT CAATATTGAT 1140

GACTTGATTA GAGACCAAGG ATTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 1200

ACCCCCCCTG CCTCATTACC TGGCTCACTA ACTAACGTGA AAGCCTTACA GAAATCTCCA 1260

GGACCTCAGC GAGAAAGGAA GTCATCTTCA TCCTCAGAAG ACAGGAATCG AATGAAAACA 1320

CTTGGTAGAC GGGACTCGAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 1380

CAAAGAATTG GATCTGGATC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 1440

GCAGTGAAAA TGTTGAATGT GACAGCACCT ACACCTCAGC AGTTACAAGC CTTCAAAAAT 1500

GAAGTAGGAG TACTCAGGAA AACACGACAT GTGAATATCC TACTCTTCAT GGGCTATTCC 1560

ACAAAGCCAC AACTGGCTAT TGTTACCCAG TGGTGTGAGG GCTCCAGCTT GTATCACCAT 1620

CTCCATATCA TTGAGACCAA ATTTGAGATG ATCAAACTTA TAGATATTGC ACGACAGACT 1680

GCACAGGGCA TGGATTACTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 1740

AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ATTTTGGTCT AGCTACAGTG 1800

AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGATCCAT TTTGTGGATG 1860

GCACCAGAAG TCATCAGAAT GCAAGATAAA AATCCATACA GCTTTCAGTC AGATGTATAT 1920

GCATTTGGGA TTGTTCTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAACATCAAC 1980

AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TGTCTCCAGA TCTCAGTAAG 2040

GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 2100

AGAGATGAGA GACCACTCTT TCCCCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 2160

TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 2220

GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 2280

GGTGCGTTTC CTGTCCACTG A 2301

Domain Profile

S: 2     elaekiGsGrfGtvyrgkwhGdvavkllnveelseeqleaFkkevaaykktRhenivLFm  61

         +++++iGsG+fGtvy+gkwhGdvavk+lnv++++++ql+aFk+ev +++ktRh+ni+LFm

Q: 458   TVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFM  517

         7899********************************************************

S: 62    GyvskpelaivtslckgssLykllheqetkldlaklidiarqiaqgmdYLhakkilhkDl  121

         Gy +kp+laivt++c+gssLy++lh  etk+++ klidiarq+aqgmdYLhak+i+h+Dl

Q: 518   GYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDL  577

         ************************************************************

S: 122   ksknilleeglkvvitDFgllsvkrllsgrrekqleipkgwilylapeviralsidleqd  181

         ks+ni+l+e+l+v+i+DFgl++vk+++sg  ++q+e+ +g+il++apevir+      qd

Q: 578   KSNNIFLHEDLTVKIGDFGLATVKSRWSG--SHQFEQLSGSILWMAPEVIRM------QD  629

         *****************************..*********************......**

S: 182   elpfskesDvyafGivlyellarelpyke.esadqilfavgrG.lkpdlskvksklpkel  239

         ++p+s++sDvyafGivlyel++++lpy++ +++dqi+f+vgrG l+pdlskv+s++pk++

Q: 630   KNPYSFQSDVYAFGIVLYELMTGQLPYSNiNNRDQIIFMVGRGyLSPDLSKVRSNCPKAM  689

         ************************************************************

S: 240   kellleClkfekeeRPlftqilkkl  264

         k+l++eClk++++eRPlf+qil+++

Q: 690   KRLMAECLKKKRDERPLFPQILASI  714

         *********************9875

Domain Sequence
(FASTA)

TVGQRIGSGS FGTVYKGKWH GDVAVKMLNV TAPTPQQLQA FKNEVGVLRK TRHVNILLFM 60

GYSTKPQLAI VTQWCEGSSL YHHLHIIETK FEMIKLIDIA RQTAQGMDYL HAKSIIHRDL 120

KSNNIFLHED LTVKIGDFGL ATVKSRWSGS HQFEQLSGSI LWMAPEVIRM QDKNPYSFQS 180

DVYAFGIVLY ELMTGQLPYS NINNRDQIIF MVGRGYLSPD LSKVRSNCPK AMKRLMAECL 240

KKKRDERPLF PQILASI 257

Keyword

ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005622	; C:intracellular
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0005057	; F:receptor signaling protein activity
GO:0035556	; P:intracellular signal transduction

KEGG

ptr:463781

;

InterPros

IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR003116	; Raf-like_ras-bd.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF07714	; Pkinase_Tyr; 1.
PF02196	; RBD; 1.

SMARTs

SM00109	; C1; 1.
SM00455	; RBD; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50898	; RBD; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013