EKS-RAN-00246

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00246

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	323.7	9.1E-97	57	307	251

Status

Reviewed

Ensembl Protein

ENSRNOP00000000039

UniProt Accession

Q66HE5;

Protein Name

NUAK family SNF1-like kinase 2

Protein Synonyms/Alias

SNF1/AMP kinase-related kinase; SNARK;

Gene Name

Nuak2

Gene Synonyms/Alias

Nuak2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000000034	ENSRNOP00000000039	ENSRNOT00000000039

Organism

Rattus norvegicus

Functional Description

Stress-activated kinase involved in tolerance to glucosestarvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95- activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).

Protein Length

630

Protein Sequence
(FASTA)

MESVALHRRG NLAPSASALA TESARPLADR LIKSPKPLMK KQAVKRHHHK HNLRHRYEFL 60

ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH IRREIEIMSS LNHPHIIAIH 120

EVFENSSKIV IVMEYASRGD LYDYISERPR LNERDARHFF RQIVSALHYC HQNGIVHRDL 180

KLENILLDAS GNIKIADFGL SNLYHKGKFL QTFCGSPLYA SPEIVNGKPY VGPEVDSWSL 240

GVLLYILVHG TMPFDGQDHK TLVKQISSGA YREPCKPSDA CGLIRWLLMV NPIRRATLED 300

VASHWWVNWG YSTRIGEQEA LREGGHPSGD SGRASMADWL RRSSRPLLEN GAKVCSFFKQ 360

HVPGGGSTGP GLERQHSLKK SRKENDMAQT LQNDPAEDTS SRPGKNSLKL PKGILKKKAS 420

PSSGEVQEGP QELRPVSNTP GQPVPAIPLL PRKGILKKSR QRESGYYSSP EPSESGELLD 480

AGDVFVSGDP MEQKSPQASG RLHRKGILKL NGKFSRTALE GTAPSTFGSL DQLASPHPTA 540

RASRPSGAVS EDSILSSESF DQLDLPERLP ETPLRGCVSV DNLRRLEQPP SEGLKRWWQE 600

SLGDSCFSLT DCQEVTAAYR QALGICSKLS 630

Nucleotide Sequence
(FASTA)

ATGGAGTCGG TGGCCTTACA CCGGCGCGGG AACCTGGCTC CCTCGGCCTC CGCCCTGGCC 60

ACGGAGAGCG CCCGGCCGCT GGCGGACCGG CTCATCAAGT CGCCCAAACC TCTGATGAAG 120

AAGCAGGCGG TGAAGCGGCA CCATCACAAA CACAACCTGA GGCACCGCTA CGAGTTCCTG 180

GAGACCCTGG GCAAGGGCAC CTACGGGAAG GTGAAGAAAG CACGAGAGAG CTCGGGACGC 240

CTGGTGGCCA TCAAGTCTAT CAGGAAGGAC AAAATCAAAG ATGAGCAGGA TCTGTTGCAC 300

ATAAGGAGGG AGATCGAGAT CATGTCTTCA CTCAACCACC CCCACATCAT TGCCATCCAT 360

GAAGTGTTTG AGAACAGCAG CAAGATTGTG ATTGTCATGG AGTACGCCAG CCGAGGCGAT 420

CTGTACGATT ACATCAGTGA GCGGCCACGG CTGAATGAGC GGGACGCCAG GCATTTCTTC 480

CGACAGATCG TGTCCGCCCT GCACTACTGC CACCAGAACG GGATTGTTCA CCGGGACCTC 540

AAGCTGGAGA ACATCCTTCT AGATGCCAGT GGCAACATCA AGATTGCTGA TTTTGGCCTC 600

TCCAACCTGT ATCACAAAGG CAAGTTCCTC CAGACGTTCT GTGGGAGCCC TCTCTATGCC 660

TCACCTGAGA TCGTCAACGG GAAGCCCTAT GTGGGCCCAG AGGTGGACAG CTGGTCTCTG 720

GGCGTTCTTC TGTACATCCT GGTGCATGGC ACCATGCCCT TTGACGGGCA GGATCATAAA 780

ACCCTGGTGA AACAAATCAG TAGCGGGGCT TACCGAGAGC CGTGCAAACC GTCTGATGCC 840

TGTGGCCTGA TCCGGTGGCT GTTAATGGTG AATCCCATCC GTCGGGCCAC TCTGGAGGAT 900

GTAGCCAGTC ATTGGTGGGT CAACTGGGGT TACAGCACCC GAATTGGGGA ACAGGAAGCT 960

CTGCGAGAGG GTGGGCACCC TAGCGGTGAC TCTGGCCGGG CCTCTATGGC GGACTGGTTA 1020

CGTCGCTCCT CCCGCCCCCT CCTGGAGAAT GGAGCCAAAG TGTGTAGCTT CTTCAAGCAG 1080

CATGTGCCGG GAGGTGGAAG CACGGGACCG GGGCTGGAGC GGCAACATTC TCTTAAGAAG 1140

TCCCGCAAGG AGAATGACAT GGCTCAGACT CTGCAGAATG ACCCAGCTGA AGATACTTCC 1200

TCTCGCCCTG GCAAGAACAG CCTCAAGCTT CCGAAAGGTA TCCTCAAGAA AAAGGCCTCT 1260

CCCTCATCGG GGGAGGTACA GGAGGGCCCT CAGGAACTCA GACCAGTGTC CAATACCCCA 1320

GGGCAGCCTG TCCCTGCTAT ACCCCTGCTC CCAAGGAAGG GCATTCTTAA GAAGTCTCGG 1380

CAGCGTGAAT CTGGTTACTA CTCCTCTCCA GAGCCCAGTG AGTCTGGGGA ACTCTTAGAC 1440

GCAGGTGATG TGTTTGTGAG TGGGGACCCC ATGGAGCAGA AGTCTCCACA AGCTTCAGGG 1500

CGCCTCCATC GCAAGGGCAT CCTCAAACTC AATGGCAAGT TTTCCCGCAC AGCCTTAGAA 1560

GGCACTGCCC CTAGCACCTT TGGCTCCCTA GACCAACTGG CCTCCCCTCA TCCTACAGCC 1620

CGGGCCAGCC GTCCCTCGGG AGCTGTGAGT GAGGACAGCA TCCTGTCCTC CGAGTCCTTT 1680

GACCAATTGG ACTTGCCCGA GCGGCTTCCC GAAACCCCAC TGAGGGGCTG TGTGTCTGTG 1740

GACAACCTGA GGAGGCTTGA GCAGCCTCCC TCAGAAGGCC TAAAACGATG GTGGCAGGAA 1800

TCCTTGGGGG ATAGCTGCTT TTCTCTGACA GACTGCCAAG AGGTGACAGC AGCCTACAGA 1860

CAAGCCCTAG GAATCTGCTC GAAGCTCAGC TGA 1893

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         y++l+tlG+g++gkVk+a+++  ++ va+K i+k+k+ +e+ l +i+rEiei+++l+hp+

Q: 57    YEFLETLGKGTYGKVKKARES-SGRLVAIKSIRKDKIkDEQDLLHIRREIEIMSSLNHPH  115

         99***************9986.799**********996667788****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         ii ++ev+e+++k+++v+eyas+g+L+dyi+e+ rl+e++ar++f+q+vsa++Y+h++gi

Q: 116   IIAIHEVFENSSKIVIVMEYASRGDLYDYISERPRLNERDARHFFRQIVSALHYCHQNGI  175

         ************************************************************

S: 120   vHrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkv  179

         vHrDlK+eN+lld+++nikiaDFGl+nl+++gk l+tfcGsp Ya+Pe+++gk+Y gp+v

Q: 176   VHRDLKLENILLDASGNIKIADFGLSNLYHKGKFLQTFCGSPLYASPEIVNGKPYVGPEV  235

         ************************************************************

S: 180   DvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpek  239

         D WslGv+Ly+lv g++Pfd+++ k+l+++i++g ++ p+k s ++  L+r lL+v+p +

Q: 236   DSWSLGVLLYILVHGTMPFDGQDHKTLVKQISSGAYREPCKPS-DACGLIRWLLMVNPIR  294

         *****************************************66.5568************

S: 240   Ratieeilkhewl  252

         Rat+e++ +h w+

Q: 295   RATLEDVASHWWV  307

         ************6

Domain Sequence
(FASTA)

YEFLETLGKG TYGKVKKARE SSGRLVAIKS IRKDKIKDEQ DLLHIRREIE IMSSLNHPHI 60

IAIHEVFENS SKIVIVMEYA SRGDLYDYIS ERPRLNERDA RHFFRQIVSA LHYCHQNGIV 120

HRDLKLENIL LDASGNIKIA DFGLSNLYHK GKFLQTFCGS PLYASPEIVN GKPYVGPEVD 180

SWSLGVLLYI LVHGTMPFDG QDHKTLVKQI SSGAYREPCK PSDACGLIRW LLMVNPIRRA 240

TLEDVASHWW V 251

Keyword

Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030036	; P:actin cytoskeleton organization
GO:0006915	; P:apoptotic process
GO:0042149	; P:cellular response to glucose starvation
GO:0043066	; P:negative regulation of apoptotic process

KEGG

rno:289419

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013