EKS-RAN-00517

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00517

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/BRD	N/A	N/A	N/A	N/A	N/A

Status

Reviewed

Ensembl Protein

ENSRNOP00000058256

UniProt Accession

D4A7T3;

Protein Name

Bromodomain testis-specific protein

Protein Synonyms/Alias

Gene Name

Brdt

Gene Synonyms/Alias

Brdt;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000002073	ENSRNOP00000058256	ENSRNOT00000061538

Organism

Rattus norvegicus

Functional Description

Testis-specific chromatin protein that specificallybinds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin (By similarity).

Protein Length

326

Protein Sequence
(FASTA)

MSLPSRQMAI VNPPPPEYIN AKKTGRLTNQ LQFLQRVVLK ALWKHSFSWP FQQPVDAAKL 60

KLPDYYTIIE TPMDLSTIKK RLENRYYEKA SECVGDFNTM FSNCYLYNKP GDDIVVMAQA 120

LEKLFMQKLS QMPQEEQIVG GKERMKKDIQ QKTAVSSAKE QTPSKSAENV FKRQEIPAGF 180

PDVCLSPLNM AQEAPPTCDS QTVVQITKGV KRRADTTTPT TSSAKASSES PPPLREAKPA 240

NAPVKENTVK SVLPDSQQQH RVLKTVKVTE QLKHCSEILK EMLAKKHLPY AWPFYNPVDV 300

DALGLHNYYD IVKNPMDLGT IKVNTA 326

Nucleotide Sequence
(FASTA)

ATGTCTCTGC CAAGTCGACA AATGGCTATT GTTAACCCCC CTCCACCAGA GTACATAAAT 60

GCTAAGAAAA CTGGGCGGTT GACCAACCAG CTGCAGTTCC TGCAGAGAGT CGTCCTGAAG 120

GCGCTGTGGA AACACAGTTT CTCCTGGCCG TTTCAACAGC CTGTGGATGC CGCGAAGCTC 180

AAGCTGCCTG ACTATTACAC CATCATAGAG ACGCCAATGG ATTTAAGTAC AATTAAGAAG 240

CGGCTGGAAA ACAGGTACTA CGAGAAGGCT TCCGAATGCG TGGGAGACTT CAACACGATG 300

TTCTCAAACT GCTACTTATA CAACAAGCCT GGAGATGACA TTGTGGTTAT GGCCCAAGCT 360

CTGGAGAAGT TATTTATGCA GAAGCTGTCT CAGATGCCAC AGGAAGAGCA GATTGTGGGT 420

GGCAAGGAGA GAATGAAGAA AGATATCCAA CAAAAGACAG CCGTTTCCTC TGCTAAGGAA 480

CAAACTCCCT CCAAATCGGC AGAAAATGTG TTTAAGCGAC AAGAAATTCC TGCTGGGTTT 540

CCTGATGTCT GTCTCTCTCC CTTAAACATG GCACAAGAGG CTCCCCCGAC CTGCGACTCA 600

CAGACTGTGG TCCAAATTAC AAAGGGTGTG AAGAGGAGGG CGGACACAAC AACTCCTACC 660

ACTTCCTCAG CTAAAGCAAG TAGTGAATCC CCTCCGCCAC TGAGAGAAGC GAAGCCGGCG 720

AATGCGCCAG TGAAGGAAAA CACAGTGAAG AGCGTCCTGC CAGACTCTCA GCAGCAACAC 780

AGGGTTTTAA AAACGGTCAA AGTAACGGAG CAGTTGAAGC ACTGCAGCGA GATTCTTAAA 840

GAAATGCTTG CCAAGAAGCA TCTGCCGTAC GCATGGCCCT TCTACAACCC TGTGGACGTG 900

GATGCTTTGG GACTCCACAA CTACTATGAC ATTGTCAAAA ACCCCATGGA TCTTGGAACG 960

ATCAAGGTAA ATACTGCCTG A 981

Domain Profile

N/A

Domain Sequence
(FASTA)

N/A

Keyword

Activator; Bromodomain; Chromatin regulator; Coiled coil; Complete proteome; Differentiation; Meiosis; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spermatogenesis; Transcription; Transcription regulation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Takifugu rubripes"; ?>Xenopus tropicalis"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0070577	; F:histone acetyl-lysine binding
GO:0030154	; P:cell differentiation
GO:0006338	; P:chromatin remodeling
GO:0001207	; P:histone displacement
GO:0007141	; P:male meiosis I
GO:0006397	; P:mRNA processing
GO:0051039	; P:positive regulation of transcription during meiosis
GO:0043484	; P:regulation of RNA splicing
GO:0008380	; P:RNA splicing
GO:0007283	; P:spermatogenesis
GO:0006351	; P:transcription, DNA-dependent

KEGG

InterPros

IPR001487	; Bromodomain.
IPR018359	; Bromodomain_CS.

Pfam

PF00439

; Bromodomain; 2.

SMARTs

SM00297

; BROMO; 2.

Prosites

PS00633	; BROMODOMAIN_1; 2.
PS50014	; BROMODOMAIN_2; 2.
PS51525	; NET; 1.

Prints

PR00503

; BROMODOMAIN.

Created Date

20-Feb-2013