EKS-RAN-00162

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00162

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKG	495.3	4.0E-149	453	711	259

Status

Reviewed

Ensembl Protein

ENSRNOP00000003237

UniProt Accession

Q64595;

Protein Name

cGMP-dependent protein kinase 2

Protein Synonyms/Alias

cGK 2; cGK2; cGMP-dependent protein kinase II; cGKII;

Gene Name

Prkg2

Gene Synonyms/Alias

Prkg2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000002361	ENSRNOP00000003237	ENSRNOT00000003237

Organism

Rattus norvegicus

Functional Description

Protein Length

762

Protein Sequence
(FASTA)

MGNGSVKPKH SKHPDGQSGN LSNEALRSKV AELEREVKRK DAELQEREYH LKELREQLAK 60

QTVAIAELTE ELQSKCIQLN KLQDVIHVQG GSPLQASPDK VPLDVHRKTS GLVSLHSRRG 120

AKAGVSAEPT SRTYDLNKPP EFSFEKARVR KDSSEKKLIT DALNKNQFLK RLDPQQIKDM 180

VECMYGRNYQ QGSYIVKQGE PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC 240

TRTASVKAIT NVKTWALDRE VFQNIMRRTA QARDEEYRNF LRSVSLLKNL PEDKLTKIID 300

CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ KGEYFGEKAL 360

ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFDELQKY LEGYVATLNR DDEKRHAKRS 420

MSSWKLSKAL SLEMIQLKEK VARFSSTSPF QNLEIIATLG VGGFGRVELV KVKNENIAFA 480

MKCIRKKHIV DTKQQEHVYS EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS 540

ILRDRGSFDE PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDADGYL KLVDFGFAKK 600

IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF SGIDQMMTYN 660

LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN GINDIKKHRW LNGFNWEGLK 720

ARSLPSPLRR ELSGPIDHSY FDKYPPEKGV PPDEMSGWDK DF 762

Nucleotide Sequence
(FASTA)

ATGGGAAATG GTTCAGTGAA ACCCAAGCAT TCGAAGCACC CAGACGGCCA GTCAGGGAAC 60

CTCAGCAATG AAGCCCTGCG GAGCAAAGTG GCAGAGCTGG AGCGCGAGGT GAAGAGGAAG 120

GATGCAGAAC TTCAGGAGCG CGAGTACCAC CTGAAGGAGC TTCGGGAGCA GCTGGCTAAG 180

CAGACCGTGG CCATTGCGGA GCTCACCGAG GAGCTGCAGA GCAAGTGCAT CCAGCTGAAC 240

AAGCTGCAGG ATGTTATCCA CGTGCAGGGA GGAAGCCCAC TGCAGGCCTC TCCCGACAAA 300

GTGCCTCTGG ATGTTCACCG CAAGACCTCA GGCTTGGTCT CCCTCCACAG CAGGAGGGGG 360

GCGAAGGCTG GAGTGTCTGC AGAGCCCACA AGCCGGACCT ATGACCTCAA CAAGCCCCCT 420

GAGTTCTCCT TTGAGAAAGC GAGGGTCAGG AAGGATTCCA GTGAGAAAAA ACTTATCACA 480

GACGCCCTGA ATAAGAACCA GTTTCTGAAG AGACTGGATC CGCAGCAGAT CAAAGACATG 540

GTGGAATGCA TGTACGGGAG AAACTATCAA CAGGGCAGTT ACATCGTTAA GCAAGGGGAA 600

CCTGGAAACC ACATCTTTGT GCTGGCCGAG GGTAGACTGG AGGTGTTCCA AGGAGAGAAA 660

TTGCTGTCGT CCATCCCAAT GTGGACCACG TTTGGGGAGC TGGCTATTTT GTATAATTGT 720

ACGCGGACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CTTGGGCTCT CGATAGAGAG 780

GTTTTCCAGA ACATTATGAG GAGAACGGCG CAAGCCAGGG ATGAGGAATA CAGAAACTTC 840

CTCAGAAGTG TCTCCTTGTT GAAGAATTTA CCTGAAGACA AACTAACCAA GATTATTGAC 900

TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTATATCA TCAGAGAAGG AGAGGAAGGG 960

AGCACCTTTT TCATCTTGGC AAAAGGAAAG GTGAAAGTCA CACAGAGCAC AGAGGGCCAT 1020

GATCAGCCAC AGTTGATCAA AACACTGCAG AAAGGAGAGT ACTTTGGAGA AAAGGCTCTG 1080

ATCAGTGATG ATGTCAGATC GGCCAATATA ATCGCGGAAG AAAATGATGT CGCATGCCTC 1140

GTGATCGACC GAGAAACATT CAACCAAACT GTCGGGACAT TTGATGAACT CCAAAAATAC 1200

CTTGAGGGGT ATGTGGCCAC CCTGAACCGT GACGATGAAA AGAGACACGC AAAGCGCTCC 1260

ATGTCTAGCT GGAAGTTGTC CAAAGCCCTT TCTCTGGAGA TGATTCAGCT GAAAGAGAAG 1320

GTGGCCAGAT TCTCCTCAAC CTCCCCATTC CAGAACCTTG AGATTATCGC AACACTGGGC 1380

GTTGGTGGGT TCGGAAGAGT GGAGCTTGTT AAAGTTAAAA ATGAGAACAT CGCTTTTGCC 1440

ATGAAGTGTA TAAGGAAGAA GCACATTGTT GACACTAAAC AGCAGGAACA TGTCTACTCC 1500

GAGAAGAGGA TCCTGGAGGA GCTGTGTTCT CCCTTCATCG TGAAATTGTA TCGTACTTTC 1560

AAGGACAATA AATACGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTCTGGAGT 1620

ATATTAAGGG ACAGAGGCAG CTTCGATGAA CCCACCTCCA AATTCTGTGT GGCCTGCGTG 1680

ACAGAAGCAT TCGATTATCT ACACCGACTA GGCATTATCT ACAGAGACCT GAAGCCAGAG 1740

AACTTAATTC TGGATGCCGA TGGCTACCTT AAGTTGGTTG ACTTTGGATT CGCTAAGAAG 1800

ATTGGCTCTG GGCAGAAAAC GTGGACATTC TGTGGGACTC CAGAGTATGT GGCCCCCGAG 1860

GTCATTCTTA ACAAAGGACA TGACTTCAGT GTGGATTTCT GGTCCCTGGG GATTCTGGTC 1920

TATGAGCTTC TAACGGGCAA CCCTCCCTTT TCTGGGATAG ACCAAATGAT GACCTACAAT 1980

TTGATTCTCA AAGGGATCGA GAAAATGGAT TTTCCTAGAA AGATAACAAG GCGACCCGAG 2040

GATTTGATCC GGAGGCTTTG CAGGCAAAAC CCAACAGAAA GGCTGGGGAA CCTGAAGAAT 2100

GGGATCAACG ACATTAAGAA ACACAGGTGG TTAAACGGTT TCAATTGGGA GGGGTTGAAA 2160

GCACGGAGCC TTCCCTCACC TTTACGAAGA GAGCTCAGTG GCCCCATAGA TCACAGCTAC 2220

TTTGACAAGT ATCCTCCTGA AAAGGGGGTT CCTCCGGATG AAATGTCAGG CTGGGATAAG 2280

GACTTCTGA 2289

Domain Profile

S: 1     lkviatlgvggfgrvelvkvksdeskafalkilkkkhivdtkqqehvlsekrileeaksd  60

         l++iatlgvggfgrvelvkvk+ e+ afa+k+++kkhivdtkqqehv+sekrilee++s+

Q: 453   LEIIATLGVGGFGRVELVKVKN-ENIAFAMKCIRKKHIVDTKQQEHVYSEKRILEELCSP  511

         689******************9.899**********************************

S: 61    fivklyrtfkdekyvyllleaclggelwtilrdrgsfddktakfivacvvealeylhskn  120

         fivklyrtfkd+kyvy+lleaclggelw+ilrdrgsfd+ t+kf+vacv+ea++ylh+ +

Q: 512   FIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLG  571

         ************************************************************

S: 121   iiyrdlkpenllldeegylklvdfgfakklksgkktwtfcgtpeyvapeiilnkghdlsv  180

         iiyrdlkpenl+ld++gylklvdfgfakk++sg+ktwtfcgtpeyvape+ilnkghd+sv

Q: 572   IIYRDLKPENLILDADGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSV  631

         ************************************************************

S: 181   dywalgilvyelltgkppfsgvdplktynlilkgidklelprkitkeaedlikklcrdnp  240

         d+w+lgilvyelltg+ppfsg+d+++tynlilkgi+k+++prkit+++edli++lcr+np

Q: 632   DFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNP  691

         ************************************************************

S: 241   aerlgylkggikdikkhkwf  260

         +erlg+lk+gi+dikkh+w+

Q: 692   TERLGNLKNGINDIKKHRWL  711

         *******************7

Domain Sequence
(FASTA)

LEIIATLGVG GFGRVELVKV KNENIAFAMK CIRKKHIVDT KQQEHVYSEK RILEELCSPF 60

IVKLYRTFKD NKYVYMLLEA CLGGELWSIL RDRGSFDEPT SKFCVACVTE AFDYLHRLGI 120

IYRDLKPENL ILDADGYLKL VDFGFAKKIG SGQKTWTFCG TPEYVAPEVI LNKGHDFSVD 180

FWSLGILVYE LLTGNPPFSG IDQMMTYNLI LKGIEKMDFP RKITRRPEDL IRRLCRQNPT 240

ERLGNLKNGI NDIKKHRWL 259

Keyword

ATP-binding; Cell membrane; cGMP; cGMP-binding; Complete proteome; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016324	; C:apical plasma membrane
GO:0005524	; F:ATP binding
GO:0030553	; F:cGMP binding
GO:0004692	; F:cGMP-dependent protein kinase activity
GO:0006468	; P:protein phosphorylation

KEGG

rno:25523

;

InterPros

IPR000961	; AGC-kinase_C.
IPR016232	; cGMP-dependent_protein_kinase.
IPR002374	; cGMP_dep_kinase.
IPR018490	; cNMP-bd-like.
IPR018488	; cNMP-bd_CS.
IPR000595	; cNMP-bd_dom.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014710	; RmlC-like_jellyroll.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00027	; cNMP_binding; 2.
PF00069	; Pkinase; 1.

SMARTs

SM00100	; cNMP; 2.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00888	; CNMP_BINDING_1; 2.
PS00889	; CNMP_BINDING_2; 2.
PS50042	; CNMP_BINDING_3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00104

; CGMPKINASE.

Created Date

20-Feb-2013