EKS-RAN-00220

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00220

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKN	543.8	7.4E-164	619	878	260

Status

Reviewed

Ensembl Protein

ENSRNOP00000005770

UniProt Accession

Q63433; Q6P748; Q8VIJ2;

Protein Name

Serine/threonine-protein kinase N1

Protein Synonyms/Alias

Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N;

Gene Name

Pkn1

Gene Synonyms/Alias

Pkn1; Pkn, Prk1, Prkcl1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000004131	ENSRNOP00000005770	ENSRNOT00000005770

Organism

Rattus norvegicus

Functional Description

PKC-related serine/threonine-protein kinase involved invarious processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170', and GFAP. Able to phosphorylate RPS6 in vitro.

Protein Length

946

Protein Sequence
(FASTA)

MAGDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLKREIRKEL KLKEGAENLR 60

RATTDLGRSL APVELLLRGS ARRLDLLHQQ LQELHAHVVL PDPTAGSDAP QSLAEGSPVC 120

SSTNLSRVAG LEKQLAIELK VKQGAENMIQ TYSNGSTKDR KLLLTAQQML QDSKTKIDII 180

RMQLRRALQA LQAGQLESQA APDEAHGDPD LGAVELRIEE LRHHFRVEHA VAEGAKNVLR 240

LLSAAKAPDR KAVSEAQEKL TESNQKLGLL RESLERRLGE LPADHPKGRL LREELTAASS 300

AAFSAILPGP FPATHYSTLS KPAPLTGTLE VRVVGCKNLP ETIPWSPPPS VGASGTPDSR 360

TPFLSRPARG LYNRSGSLSG RSSLKGEAEN STEVSTVLKL DNTVVGQTAW KPCGPNAWDQ 420

SFTLELERAR ELELAVFWRD QRGLCALKFL KLEDFLDNER HEVQLDMEPQ GCLVAEVTFR 480

NPIIERIPRL QRQKKIFSKQ QGQTFQRARQ MNIDVATWVR LLRRLIPNAV ATGSFSPNAS 540

PGSEIRSTGD ISMEKLNLGA DSDSSSQKSP AGLPSTSCSL SSPTHESTTS PELPSETQET 600

PGPGLCSPLR KSPLTLEDFK FLAVLGRGHF GKVLLSEFHS SGELFAIKAL KKGDIVARDE 660

VESLMCEKRI LATVTRAGHP FLVNLFGCFQ TPEHVCFVME YSAGGDLMLH IHSDVFSEPR 720

AVFYSACVVL GLQFLHEHKI VYRDLKLDNL LLDTEGYVKI ADFGLCKEGM GYGDRTSTFC 780

GTPEFLAPEV LTDTSYTRAV DWWGLGVLLY EMLVGESPFP GDDEEEVFDS IVNDEVRYPR 840

FLSAEAIGIM RRLLRRNPER RLGSTERDAE DVKKQPFFRT LDWDALLARR LPPPFVPTLS 900

GRTDVSNFDE EFTGEAPTLS PPRDARPLTA AEQAAFRDFD FVAGGY 946

Nucleotide Sequence
(FASTA)

ATGGCCGGCG ACGCCGTGCA GAGTGAACCT CGCAGCTGGT CACTGCTGGA GCAGCTGGGT 60

CTGGCTGGGG CAGACCTGGC AGCCCCTGGG GTGCAGCAGC AGCTGGAGTT AGAGCGAGAG 120

CGGCTGAAGC GGGAAATCCG AAAAGAGCTG AAGCTGAAGG AGGGCGCTGA GAACCTGAGG 180

CGGGCCACCA CTGACCTGGG CCGCAGCTTG GCCCCTGTGG AACTGCTGCT GAGGGGCTCC 240

GCTAGACGGC TTGACTTGCT GCACCAGCAG CTGCAGGAGC TGCATGCACA TGTGGTGCTG 300

CCCGACCCTA CAGCGGGGAG TGATGCTCCC CAATCCCTTG CAGAGGGCAG CCCTGTCTGC 360

TCATCCACCA ACCTGAGCCG AGTGGCTGGC CTGGAGAAGC AGCTGGCCAT TGAGCTCAAG 420

GTCAAACAGG GGGCAGAAAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 480

AAGCTGCTGT TGACGGCCCA ACAGATGCTG CAGGATAGTA AGACCAAGAT TGACATCATC 540

CGCATGCAGC TTCGCCGGGC GCTACAAGCA CTCCAGGCTG GCCAGCTGGA GAGTCAGGCA 600

GCTCCTGATG AGGCCCACGG AGATCCAGAC CTGGGAGCCG TAGAGCTACG CATTGAGGAG 660

CTACGACACC ATTTTCGAGT AGAGCATGCA GTGGCAGAAG GCGCCAAGAA TGTCCTGCGT 720

CTGCTCAGTG CTGCAAAGGC CCCAGACCGC AAAGCAGTCA GCGAGGCTCA GGAGAAATTG 780

ACTGAGTCCA ACCAGAAGCT GGGCTTGCTG CGGGAGTCAC TGGAGAGGCG CTTGGGGGAA 840

CTGCCTGCTG ATCACCCCAA GGGACGCCTG CTTCGGGAGG AGCTCACTGC GGCCTCATCG 900

GCAGCCTTCA GTGCAATACT GCCTGGGCCC TTCCCTGCCA CTCACTACAG CACCTTGAGC 960

AAGCCTGCAC CACTCACAGG GACCCTGGAA GTACGAGTGG TGGGCTGCAA AAACCTTCCC 1020

GAGACCATCC CTTGGAGCCC TCCCCCCTCA GTCGGGGCAT CTGGGACCCC CGACAGCCGC 1080

ACTCCTTTCC TGAGTCGTCC AGCTCGGGGC CTTTACAACC GAAGTGGAAG CCTTAGTGGA 1140

CGGAGCAGCC TCAAGGGGGA GGCAGAGAAT TCCACTGAGG TCAGCACCGT GCTCAAGCTG 1200

GACAACACTG TGGTGGGGCA AACAGCCTGG AAGCCATGCG GCCCCAATGC CTGGGACCAG 1260

AGCTTCACCC TGGAGCTGGA GAGGGCTCGG GAGCTGGAGT TGGCTGTGTT CTGGCGTGAC 1320

CAGAGGGGTC TGTGTGCTCT CAAATTTCTG AAGTTGGAAG ACTTCTTGGA CAATGAGAGG 1380

CATGAGGTGC AGCTGGACAT GGAACCCCAG GGCTGCCTGG TGGCTGAGGT CACCTTCCGT 1440

AACCCCATCA TCGAGCGGAT CCCTAGGCTC CAAAGGCAGA AAAAAATTTT CTCCAAGCAG 1500

CAAGGGCAGA CATTTCAGCG TGCCAGACAG ATGAACATCG ATGTGGCCAC CTGGGTGCGG 1560

CTGCTCCGGA GACTCATCCC GAACGCCGTG GCCACTGGCT CCTTCAGCCC CAATGCATCT 1620

CCAGGCTCTG AGATCCGGAG CACTGGAGAC ATATCCATGG AGAAATTGAA TCTCGGTGCT 1680

GACTCAGACA GCTCGTCCCA GAAGAGCCCC GCAGGGCTGC CCTCCACCTC ATGTAGCCTG 1740

AGTTCTCCAA CCCACGAATC CACCACCTCT CCAGAGCTGC CTTCAGAGAC CCAGGAGACC 1800

CCAGGCCCTG GCCTGTGCAG TCCCCTGAGG AAGTCGCCCC TGACGCTTGA GGACTTCAAG 1860

TTCCTGGCAG TGCTTGGTCG GGGTCACTTT GGAAAGGTGC TGCTGTCTGA ATTCCACTCC 1920

AGTGGGGAGC TCTTTGCCAT TAAAGCCTTG AAGAAAGGTG ACATTGTAGC CCGGGATGAG 1980

GTTGAGAGCC TGATGTGTGA GAAGCGGATT TTGGCGACCG TGACCAGGGC AGGACATCCC 2040

TTCCTGGTGA ACCTTTTCGG CTGTTTCCAG ACCCCAGAGC ATGTGTGCTT TGTGATGGAG 2100

TACTCAGCCG GTGGGGACTT GATGCTGCAT ATCCACAGCG ACGTGTTCTC AGAGCCTCGG 2160

GCTGTCTTCT ATTCGGCCTG TGTGGTGCTG GGACTGCAGT TCCTCCATGA ACACAAGATT 2220

GTCTACAGGG ACCTGAAGTT GGACAATTTG CTCCTGGATA CTGAGGGCTA CGTCAAGATC 2280

GCAGACTTTG GCCTCTGCAA GGAGGGGATG GGCTATGGGG ACCGGACCAG CACATTCTGC 2340

GGAACTCCGG AGTTCCTGGC GCCGGAAGTG CTCACAGACA CATCCTACAC TCGAGCCGTG 2400

GACTGGTGGG GACTGGGTGT ATTGCTCTAT GAGATGCTGG TTGGAGAGTC TCCGTTCCCT 2460

GGGGACGACG AGGAGGAAGT ATTTGACAGC ATCGTCAATG ATGAGGTTCG TTATCCCCGC 2520

TTCCTGTCTG CGGAGGCCAT CGGCATCATG AGAAGGCTAC TGCGGAGGAA CCCAGAGCGG 2580

AGGTTGGGAT CCACTGAGCG TGATGCAGAA GATGTGAAAA AACAGCCTTT CTTCAGGACT 2640

CTGGACTGGG ATGCCCTGCT GGCCCGTCGC CTGCCTCCAC CCTTCGTGCC TACACTTTCG 2700

GGGCGCACAG ACGTCAGCAA CTTCGATGAG GAGTTCACTG GGGAGGCCCC CACACTGAGC 2760

CCTCCCCGGG ATGCACGGCC CCTGACAGCT GCGGAGCAGG CGGCCTTCCG GGATTTCGAC 2820

TTTGTGGCAG GAGGCTATTA G 2841

Domain Profile

S: 1     frllavlgrghfgkvllseykstgelyaikalkkgdilardevesllcekrileavssvr  60

         f++lavlgrghfgkvllse++s+gel+aikalkkgdi+ardevesl+cekril++v++++

Q: 619   FKFLAVLGRGHFGKVLLSEFHSSGELFAIKALKKGDIVARDEVESLMCEKRILATVTRAG  678

         89**********************************************************

S: 61    hpflvnllacfqtkehvcfvmeyaaggdlmlhihedvfsepravfyaacvvlglqflhek  120

         hpflvnl++cfqt ehvcfvmey+aggdlmlhih+dvfsepravfy+acvvlglqflhe+

Q: 679   HPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEH  738

         ************************************************************

S: 121   kivyrdlkldnllldtegyvkiadfglckeglgygdrtstfcgtpeflapevltetsytr  180

         kivyrdlkldnllldtegyvkiadfglckeg+gygdrtstfcgtpeflapevlt+tsytr

Q: 739   KIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTR  798

         ************************************************************

S: 181   avdwwglgvllyemlvgespfpgddeeevfdsivndevryprflsveaieilrrllrknp  240

         avdwwglgvllyemlvgespfpgddeeevfdsivndevryprfls+eai+i+rrllr+np

Q: 799   AVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNP  858

         ************************************************************

S: 241   ekrlgaserdaedvkkqpff  260

         e+rlg++erdaedvkkqpff

Q: 859   ERRLGSTERDAEDVKKQPFF  878

         *******************9

Domain Sequence
(FASTA)

FKFLAVLGRG HFGKVLLSEF HSSGELFAIK ALKKGDIVAR DEVESLMCEK RILATVTRAG 60

HPFLVNLFGC FQTPEHVCFV MEYSAGGDLM LHIHSDVFSE PRAVFYSACV VLGLQFLHEH 120

KIVYRDLKLD NLLLDTEGYV KIADFGLCKE GMGYGDRTST FCGTPEFLAP EVLTDTSYTR 180

AVDWWGLGVL LYEMLVGESP FPGDDEEEVF DSIVNDEVRY PRFLSAEAIG IMRRLLRRNP 240

ERRLGSTERD AEDVKKQPFF 260

Keyword

Acetylation; ATP-binding; Cell membrane; Chromatin regulator; Complete proteome; Cytoplasm; Direct protein sequencing; Endosome; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Felis catus"; ?>Gadus morhua"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>

Gene Ontology

GO:0032154	; C:cleavage furrow
GO:0005768	; C:endosome
GO:0030496	; C:midbody
GO:0005634	; C:nucleus
GO:0005886	; C:plasma membrane
GO:0050681	; F:androgen receptor binding
GO:0005524	; F:ATP binding
GO:0003682	; F:chromatin binding
GO:0017049	; F:GTP-Rho binding
GO:0042393	; F:histone binding
GO:0042826	; F:histone deacetylase binding
GO:0035402	; F:histone kinase activity (H3-T11 specific)
GO:0030374	; F:ligand-dependent nuclear receptor transcription coactivator activity
GO:0004697	; F:protein kinase C activity
GO:0048365	; F:Rac GTPase binding
GO:0010631	; P:epithelial cell migration
GO:2000145	; P:regulation of cell motility
GO:0006357	; P:regulation of transcription from RNA polymerase II promoter
GO:0007165	; P:signal transduction
GO:0006351	; P:transcription, DNA-dependent

KEGG

rno:29355

;

InterPros

IPR000961	; AGC-kinase_C.
IPR000008	; C2_Ca-dep.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR011072	; HR1_rho-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF02185	; HR1; 3.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00239	; C2; 1.
SM00742	; Hr1; 3.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013