EKS-RAN-00274

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00274

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PDK1	422.5	9.6E-127	85	345	261

Status

Reviewed

Ensembl Protein

ENSRNOP00000061683

UniProt Accession

O55173; G3V9W3;

Protein Name

3-phosphoinositide-dependent protein kinase 1

Protein Synonyms/Alias

Protein kinase B kinase; PkB kinase;

Gene Name

Pdpk1

Gene Synonyms/Alias

Pdpk1; Pdk1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000006136	ENSRNOP00000061683	ENSRNOT00000067660

Organism

Rattus norvegicus

Functional Description

Serine/threonine kinase which acts as a master kinase,phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF- kappa-B activation in macrophages (By similarity).

Protein Length

559

Protein Sequence
(FASTA)

MARTTSQLYD AVPIQSSVVL CSCPSPSMVR SQTEPSSSPG IPSGVSRQGS TMDGTTAEAR 60

PSTNPLQQHP AQLPPQPRKK RPEDFKFGKI LGEGSFSTVV LARELATSRE YAIKILEKRH 120

IIKENKVPYV TRERDVMSRL DHPFFVKLYF TFQDDEKLYF GLSYAKNGEL LKYIRKIGSF 180

DETCTRFYTA EIVSALEYLH GKGIIHRDLK PENILLNEDM HIQITDFGTA KVLSPDSKQA 240

RANSFVGTAQ YVSPELLTEK SACKSSDLWA LGCIIYQLVA GLPPFRAGNE YLIFQKIIKL 300

EYDFPEKFFP KARDLVEKLL VLDATKRLGC EEMEGYGPLK AHPFFESITW ENLHQQTPPK 360

LTAYLPAMSE DDEDCYGNYD NLLSQFGCMQ VSSSSSSHSL SAVDASLPQR SGSNIEQYIH 420

DLDTNSFELD LQFSEDEKRL LLEKQAGGNP WHQFVENNLI LKMGPVDKRK GLFARRRQLL 480

LTEGPHLYYV DPVNKVLKGE IPWSQELRPE AKNFKTFFVH TPNRTYYLMD PSGNAHKWCR 540

KIQEVWRQQY QSSPDAAVQ 559

Nucleotide Sequence
(FASTA)

ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCTGTGCCCA TTCAGTCCAG TGTGGTGCTA 60

TGTTCCTGCC CATCCCCATC AATGGTGAGG TCCCAGACTG AGCCCAGCTC GTCCCCTGGC 120

ATTCCTAGTG GTGTTAGCAG GCAGGGATCC ACCATGGACG GCACCACAGC TGAAGCCCGA 180

CCAAGCACCA ACCCCTTGCA GCAGCACCCT GCCCAGCTGC CACCACAGCC TCGCAAGAAA 240

CGCCCTGAAG ACTTCAAGTT TGGGAAAATT CTTGGCGAGG GCTCTTTTTC GACAGTTGTT 300

CTGGCCCGAG AACTGGCCAC TTCCAGAGAA TATGCTATTA AAATTCTGGA GAAACGTCAT 360

ATAATAAAAG AAAACAAAGT CCCGTATGTA ACTAGAGAGA GAGATGTGAT GTCACGCCTG 420

GATCACCCCT TCTTTGTGAA ACTTTACTTT ACGTTTCAGG ACGACGAAAA GCTGTATTTT 480

GGCCTTAGTT ATGCCAAAAA TGGAGAGCTA CTCAAATACA TCCGCAAAAT CGGTTCATTT 540

GATGAGACCT GTACCCGGTT TTACACAGCT GAGATTGTGT CTGCTTTAGA GTACTTGCAT 600

GGCAAGGGCA TCATTCACAG AGACCTTAAA CCAGAAAACA TCTTGTTAAA CGAAGACATG 660

CACATCCAGA TCACAGATTT TGGAACAGCC AAAGTATTAT CCCCAGACAG CAAACAAGCT 720

AGAGCCAATT CATTTGTAGG AACAGCGCAG TATGTTTCTC CAGAGCTGCT CACAGAGAAG 780

TCGGCGTGTA AAAGTTCAGA CCTTTGGGCC CTTGGATGTA TAATCTATCA GCTTGTGGCA 840

GGACTTCCAC CATTCAGAGC CGGAAATGAA TATCTTATAT TTCAGAAGAT CATTAAGTTG 900

GAATATGATT TCCCAGAAAA ATTCTTCCCT AAGGCAAGAG ATCTTGTGGA AAAACTCTTG 960

GTTTTAGATG CCACAAAGCG TTTAGGCTGT GAAGAGATGG AAGGGTACGG GCCTCTCAAA 1020

GCTCATCCAT TCTTTGAGTC CATCACGTGG GAGAATCTGC ACCAGCAGAC GCCTCCGAAG 1080

CTCACAGCTT ACTTGCCAGC CATGTCAGAA GATGATGAAG ACTGCTATGG CAATTACGAC 1140

AATCTCCTGA GCCAGTTTGG CTGCATGCAG GTGTCATCCT CCTCCTCTTC CCACTCCCTG 1200

TCTGCGGTGG ATGCCAGCCT GCCCCAGAGG TCGGGCAGCA ACATAGAGCA GTACATTCAT 1260

GATTTGGACA CTAACTCTTT TGAACTAGAC TTACAGTTTT CAGAAGATGA AAAAAGGTTG 1320

TTATTGGAAA AGCAAGCCGG TGGAAACCCT TGGCACCAGT TTGTAGAAAA TAATCTAATA 1380

TTAAAAATGG GCCCAGTGGA TAAGCGAAAG GGTTTATTTG CAAGACGACG GCAGTTATTA 1440

CTCACAGAAG GGCCACATTT ATATTATGTC GATCCTGTCA ACAAGGTCTT AAAAGGTGAA 1500

ATTCCATGGT CACAAGAACT CCGACCAGAG GCCAAGAATT TTAAAACTTT CTTCGTCCAC 1560

ACGCCTAACA GGACGTATTA CCTGATGGAT CCAAGCGGGA ATGCTCACAA GTGGTGCAGA 1620

AAGATCCAGG AGGTTTGGAG GCAACAGTAC CAGAGCAGTC CAGATGCTGC TGTGCAGTGA 1680

Domain Profile

S: 1     fkfekelgeGsystvvlarekeskkeyaikvlekryiikekkvkyvtrekevlaklnehp  60

         fkf+k+lgeGs+stvvlare+++++eyaik+lekr+iike+kv+yvtre++v+++l +hp

Q: 85    FKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRL-DHP  143

         89******************************************************.9**

S: 61    givkLyatfqDeeslyfvleyakkGdllellkkvgsfdeactryyaaellsaleflhkkg  120

         ++vkLy+tfqD+e+lyf l+yak+G+ll++++k+gsfde+ctr+y+ae++sale+lh kg

Q: 144   FFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKG  203

         ************************************************************

S: 121   iihrDlkpeniLldkdgkilitDfGsakvlepklkedskkeeeesarsksfvGtaeyvsP  180

         iihrDlkpeniLl++d++i+itDfG+akvl+p++k+         ar++sfvGta+yvsP

Q: 204   IIHRDLKPENILLNEDMHIQITDFGTAKVLSPDSKQ---------ARANSFVGTAQYVSP  254

         ******************************999998.........9**************

S: 181   elLtdsevskssDlWalgcilyqllaGkpPfraeneylifkkilkleyafpegfpekakd  240

         elLt+++++kssDlWalgci+yql+aG+pPfra neylif+ki+kley+fpe+f++ka+d

Q: 255   ELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARD  314

         ************************************************************

S: 241   lveklLvkdakerltlee......ikahkff  265

         lveklLv da++rl++ee      +kah+ff

Q: 315   LVEKLLVLDATKRLGCEEmegygpLKAHPFF  345

         ******************************9

Domain Sequence
(FASTA)

FKFGKILGEG SFSTVVLARE LATSREYAIK ILEKRHIIKE NKVPYVTRER DVMSRLDHPF 60

FVKLYFTFQD DEKLYFGLSY AKNGELLKYI RKIGSFDETC TRFYTAEIVS ALEYLHGKGI 120

IHRDLKPENI LLNEDMHIQI TDFGTAKVLS PDSKQARANS FVGTAQYVSP ELLTEKSACK 180

SSDLWALGCI IYQLVAGLPP FRAGNEYLIF QKIIKLEYDF PEKFFPKARD LVEKLLVLDA 240

TKRLGCEEME GYGPLKAHPF F 261

Keyword

Acetylation; ATP-binding; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0016023	; C:cytoplasmic membrane-bounded vesicle
GO:0005829	; C:cytosol
GO:0005925	; C:focal adhesion
GO:0005634	; C:nucleus
GO:0005886	; C:plasma membrane
GO:0004676	; F:3-phosphoinositide-dependent protein kinase activity
GO:0005524	; F:ATP binding
GO:0005158	; F:insulin receptor binding
GO:0019901	; F:protein kinase binding
GO:0032148	; P:activation of protein kinase B activity
GO:0032869	; P:cellular response to insulin stimulus
GO:0048041	; P:focal adhesion assembly
GO:0006972	; P:hyperosmotic response
GO:0035556	; P:intracellular signal transduction
GO:0010667	; P:negative regulation of cardiac muscle cell apoptotic process
GO:0006469	; P:negative regulation of protein kinase activity
GO:0034122	; P:negative regulation of toll-like receptor signaling pathway
GO:0030512	; P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0018107	; P:peptidyl-threonine phosphorylation
GO:0090004	; P:positive regulation of establishment of protein localization to plasma membrane
GO:0010594	; P:regulation of endothelial cell migration
GO:0043122	; P:regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043304	; P:regulation of mast cell degranulation
GO:0006355	; P:regulation of transcription, DNA-dependent
GO:0006351	; P:transcription, DNA-dependent
GO:0003323	; P:type B pancreatic cell development

KEGG

rno:81745

;

InterPros

IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50003	; PH_DOMAIN; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013