EKS-RAN-00004

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00004

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Eph	516.1	1.6E-155	633	889	257

Status

Reviewed

Ensembl Protein

ENSRNOP00000057749

UniProt Accession

P54759;

Protein Name

Ephrin type-A receptor 7

Protein Synonyms/Alias

EPH homology kinase 3; EHK-3;

Gene Name

Epha7

Gene Synonyms/Alias

Epha7; Ehk-3, Ehk3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000007030	ENSRNOP00000057749	ENSRNOT00000061033
ENSRNOG00000007030	ENSRNOP00000009899	ENSRNOT00000009899

Organism

Rattus norvegicus

Functional Description

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.

Protein Length

998

Protein Sequence
(FASTA)

MVVQTRYPSW IILCYIWLLG FAHTGEAQAA KEVLLLDSKA QQTELEWISS PPSGWEEISG 60

LDENYTPIRT YQVCQVMEPN QNNWLRTNWI SKGNAQRIFV ELKFTLRDCN SLPGVLGTCK 120

ETFNLYYYET DYDTGRNIRE NLYVKIDTIA ADESFTQGDL GERKMKLNTE VREIGPLSKK 180

GFYLAFQDVG ACIALVSVKV YYKKCWSIIE NLAVFPDTVT GSEFSSLVEV RGTCVSSAEE 240

EAENSPRMHC SAEGEWLVPI GKCICKAGYQ QKGDTCEPCG RRFYKSSSQD LQCSRCPTHS 300

FSDREGSSRC ECEDGYYRAP SDPPYVACTR PPSAPQNLIF NINQTTVSLE WSPPADNGGR 360

NDVTYRILCK RCSWEQGECV PCGSNIGYMP QQTGLEDNYV TVMDLLAHAN YTFEVEAVNG 420

VSDLSRSQRL FAAVSITTGQ AAPSQVSGVM KERVLQRSVE LSWQEPEHPN GVITEYEIKY 480

YEKDQRERTY STLKTKSTSA SINNLKPGTV YVFQIRAFTA AGYGNYSPRL DVATLEEASG 540

KMFEATAVSS EQNPVIIIAV VAVAGTIILV FMVFGFIIGR RHCGYSKADQ EGDEELYFHS 600

LVTNEHLSVL PETYEDPNRA VHQFAKELDA SCIKIERVIG AGEFGEVCSG RLKLPGKRDV 660

AVAIKTLKVG YTEKQRRDFL CEASIMGQFD HPNVVHLEGV VTRGKPVMIV IEFMENGALD 720

AFLRKHDGQF TVIQLVGMLR GIAAGMRYLA DMGYVHRDLA ARNILVNSNL VCKVSDFGLS 780

RVIEDDPEAV YTTTGGKIPV RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM 840

SNQDVIKAIE EGYRLPAPMD CPAGLHQLML DCWQKERAER PKFEQIVGIL DKMIRNPSSL 900

KTPLGTCSRP ISPLLDQSTP DFTAFCSVGE WLQAIKMERY KDNFTAAGYN SLESVARMTI 960

DDVMSLGITL VGHQKKIMSS IQTMRAQMLH LHGTGIQV 998

Nucleotide Sequence
(FASTA)

ATGGTTGTTC AAACTCGGTA CCCTTCGTGG ATTATTTTGT GTTACATCTG GCTGCTTGGC 60

TTTGCACACA CGGGGGAGGC GCAGGCTGCG AAGGAAGTAC TGTTACTGGA CTCGAAAGCA 120

CAACAAACAG AATTGGAATG GATTTCCTCT CCACCCAGTG GGTGGGAAGA AATTAGTGGT 180

TTGGATGAGA ACTACACACC AATAAGAACA TACCAGGTGT GCCAGGTCAT GGAGCCCAAC 240

CAGAACAACT GGCTTCGGAC TAACTGGATT TCTAAAGGCA ACGCACAAAG GATTTTTGTA 300

GAATTGAAAT TCACCTTGAG GGATTGTAAT AGTCTTCCCG GAGTCCTGGG AACTTGCAAG 360

GAAACCTTTA ATTTGTACTA TTATGAAACA GACTACGACA CCGGCAGGAA TATACGAGAA 420

AACCTTTACG TTAAAATAGA CACCATTGCT GCAGATGAAA GTTTTACACA AGGTGACCTT 480

GGTGAAAGAA AGATGAAGCT GAACACTGAG GTGAGAGAGA TTGGACCTTT GTCCAAAAAG 540

GGATTCTATC TTGCCTTTCA GGATGTAGGG GCTTGCATAG CATTGGTTTC TGTCAAAGTA 600

TACTACAAGA AGTGCTGGTC CATTATTGAG AACTTAGCTG TCTTTCCAGA TACAGTGACT 660

GGTTCAGAAT TTTCCTCCTT AGTTGAGGTC CGTGGGACAT GTGTCAGCAG TGCCGAGGAA 720

GAGGCAGAAA ATTCACCCAG GATGCATTGC AGTGCAGAAG GAGAGTGGTT AGTACCCATT 780

GGAAAATGTA TCTGCAAAGC AGGCTATCAG CAAAAAGGGG ACACTTGTGA ACCCTGTGGC 840

CGCAGGTTCT ACAAATCTTC CTCTCAGGAC CTTCAGTGTT CTCGTTGTCC AACCCACAGT 900

TTCTCTGACC GAGAAGGATC ATCGAGGTGT GAATGTGAAG ACGGGTACTA CAGAGCTCCT 960

TCTGACCCAC CATACGTCGC ATGCACGAGG CCTCCCTCTG CACCACAGAA CCTTATTTTC 1020

AACATCAATC AAACGACTGT AAGTTTGGAG TGGAGTCCTC CAGCTGACAA CGGAGGAAGA 1080

AACGATGTCA CCTACAGAAT ATTGTGTAAG CGGTGCAGCT GGGAGCAGGG AGAATGTGTA 1140

CCATGCGGAA GTAACATTGG ATACATGCCC CAGCAGACGG GATTAGAGGA TAACTATGTC 1200

ACTGTCATGG ACCTGCTTGC CCATGCAAAT TACACGTTCG AAGTTGAAGC TGTAAATGGA 1260

GTTTCAGACT TAAGCAGATC CCAGAGGCTT TTTGCAGCCG TTAGCATCAC CACTGGTCAA 1320

GCAGCTCCCT CGCAAGTGAG CGGAGTGATG AAGGAGAGAG TGCTGCAGCG GAGTGTGGAG 1380

CTTTCCTGGC AGGAACCAGA ACATCCCAAC GGAGTCATCA CGGAATATGA AATCAAGTAT 1440

TATGAGAAAG ATCAAAGAGA AAGGACGTAC TCAACACTGA AAACCAAGTC CACCTCAGCC 1500

TCCATTAATA ATCTGAAACC GGGAACAGTG TATGTTTTTC AGATCCGGGC GTTCACTGCT 1560

GCCGGCTATG GAAATTACAG CCCTAGGCTG GATGTTGCAA CACTAGAGGA AGCTTCAGGT 1620

AAAATGTTTG AAGCAACAGC GGTGTCCAGT GAACAGAATC CCGTCATCAT AATTGCTGTG 1680

GTGGCTGTAG CAGGGACCAT CATCTTGGTG TTCATGGTGT TCGGCTTCAT CATTGGAAGA 1740

AGGCACTGTG GTTACAGCAA GGCTGACCAA GAAGGGGATG AAGAACTCTA CTTTCATTCT 1800

TTAGTAACAA ATGAGCATCT GTCAGTTTTA CCTGAAACCT ATGAGGACCC AAATAGAGCT 1860

GTCCATCAAT TCGCCAAGGA GCTAGATGCC TCCTGTATTA AAATTGAGCG TGTGATTGGT 1920

GCAGGAGAAT TTGGAGAAGT TTGCAGTGGT CGTTTGAAAC TTCCAGGGAA AAGAGATGTT 1980

GCAGTGGCTA TAAAAACCCT GAAAGTTGGT TACACAGAAA AACAAAGGAG GGACTTTTTA 2040

TGCGAAGCAA GCATCATGGG GCAATTTGAC CACCCAAATG TCGTCCATTT GGAAGGGGTT 2100

GTTACAAGAG GGAAGCCTGT CATGATCGTG ATAGAGTTCA TGGAGAACGG CGCCCTGGAT 2160

GCATTTCTCA GGAAACATGA TGGGCAATTT ACAGTCATTC AGTTGGTAGG AATGCTGAGA 2220

GGAATTGCGG CTGGCATGCG CTACTTGGCT GACATGGGAT ACGTTCACAG GGACCTTGCG 2280

GCTCGCAACA TTCTTGTGAA CAGCAATCTT GTTTGTAAAG TGTCCGATTT TGGCCTGTCC 2340

CGGGTTATAG AGGATGACCC CGAAGCTGTC TACACGACTA CTGGTGGAAA AATTCCAGTA 2400

AGGTGGACTG CCCCTGAAGC CATCCAATAC CGGAAATTCA CCTCAGCCAG TGATGTGTGG 2460

AGCTATGGGA TTGTCATGTG GGAAGTAATG TCTTATGGAG AACGACCTTA TTGGGACATG 2520

TCGAATCAAG ATGTCATTAA AGCGATAGAA GAAGGTTATC GCTTACCAGC ACCCATGGAC 2580

TGCCCAGCTG GTCTTCACCA GCTAATGCTG GATTGTTGGC AGAAAGAACG GGCAGAGAGG 2640

CCAAAGTTTG AGCAGATAGT CGGAATTCTA GACAAAATGA TTCGAAATCC AAGCAGTCTG 2700

AAAACACCCC TGGGAACTTG TAGCAGACCC ATAAGCCCTC TTCTGGACCA GAGCACTCCC 2760

GACTTCACTG CCTTCTGTTC AGTGGGAGAG TGGTTGCAGG CTATTAAAAT GGAGAGGTAT 2820

AAAGACAACT TCACAGCGGC AGGTTACAAC TCACTCGAGT CGGTGGCCCG GATGACCATC 2880

GACGATGTGA TGAGTTTAGG GATCACACTG GTTGGCCATC AAAAGAAGAT TATGAGCAGC 2940

ATCCAGACTA TGCGGGCACA GATGCTGCAC TTACACGGAA CCGGCATCCA AGTGTGA 2997

Domain Profile

S: 1     vkiekviGaGefGevcsGrlklpgkreiavaiktlkagstekqrrdflseasilGqfdhp  60

         +kie+viGaGefGevcsGrlklpgkr++avaiktlk g+tekqrrdfl+easi+Gqfdhp

Q: 633   IKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHP  692

         79**********************************************************

S: 61    niirleGvvtkskpvmivteymengaldsflrkkdgqftviqlvgmlrGiasgmkylsdm  120

         n+++leGvvt++kpvmiv+e+mengald+flrk+dgqftviqlvgmlrGia+gm+yl+dm

Q: 693   NVVHLEGVVTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADM  752

         ************************************************************

S: 121   gyvhrdlaarnilvnsnlvckvsdfGlsrvleddseaayttsggkipirwtapeaiayrk  180

         gyvhrdlaarnilvnsnlvckvsdfGlsrv+edd+ea+ytt+ggkip+rwtapeai+yrk

Q: 753   GYVHRDLAARNILVNSNLVCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRK  812

         ************************************************************

S: 181   ftsasdvwsyGivlwevlsyGerpywdmsnqdvikaieegyrlpppmdcpaalyqlmldc  240

         ftsasdvwsyGiv+wev+syGerpywdmsnqdvikaieegyrlp+pmdcpa+l+qlmldc

Q: 813   FTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDC  872

         ************************************************************

S: 241   wqkdraerpkfeqivsi  257

         wqk+raerpkfeqiv i

Q: 873   WQKERAERPKFEQIVGI  889

         **************987

Domain Sequence
(FASTA)

IKIERVIGAG EFGEVCSGRL KLPGKRDVAV AIKTLKVGYT EKQRRDFLCE ASIMGQFDHP 60

NVVHLEGVVT RGKPVMIVIE FMENGALDAF LRKHDGQFTV IQLVGMLRGI AAGMRYLADM 120

GYVHRDLAAR NILVNSNLVC KVSDFGLSRV IEDDPEAVYT TTGGKIPVRW TAPEAIQYRK 180

FTSASDVWSY GIVMWEVMSY GERPYWDMSN QDVIKAIEEG YRLPAPMDCP AGLHQLMLDC 240

WQKERAERPK FEQIVGI 257

Keyword

Alternative splicing; Apoptosis; ATP-binding; Cell membrane; Complete proteome; Developmental protein; Glycoprotein; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0030425	; C:dendrite
GO:0005887	; C:integral to plasma membrane
GO:0031594	; C:neuromuscular junction
GO:0043025	; C:neuronal cell body
GO:0045211	; C:postsynaptic membrane
GO:0005524	; F:ATP binding
GO:0008046	; F:axon guidance receptor activity
GO:0045499	; F:chemorepellent activity
GO:0046875	; F:ephrin receptor binding
GO:0005004	; F:GPI-linked ephrin receptor activity
GO:0007420	; P:brain development
GO:0048755	; P:branching morphogenesis of a nerve
GO:0043525	; P:positive regulation of neuron apoptotic process
GO:0022407	; P:regulation of cell-cell adhesion
GO:0043281	; P:regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0070372	; P:regulation of ERK1 and ERK2 cascade
GO:0050730	; P:regulation of peptidyl-tyrosine phosphorylation
GO:0031952	; P:regulation of protein autophosphorylation
GO:0031290	; P:retinal ganglion cell axon guidance

KEGG

rno:171287

;

InterPros

IPR001090	; Ephrin_rcpt_lig-bd_dom.
IPR003961	; Fibronectin_type3.
IPR008979	; Galactose-bd-like.
IPR009030	; Growth_fac_rcpt.
IPR013783	; Ig-like_fold.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR021129	; SAM_type1.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016257	; Tyr_kinase_ephrin_rcpt.
IPR001426	; Tyr_kinase_rcpt_V_CS.

Pfam

PF01404	; Ephrin_lbd; 1.
PF00041	; fn3; 2.
PF07714	; Pkinase_Tyr; 1.
PF00536	; SAM_1; 1.

SMARTs

SM00615	; EPH_lbd; 1.
SM00060	; FN3; 2.
SM00454	; SAM; 1.
SM00219	; TyrKc; 1.

Prosites

PS01186	; EGF_2; UNKNOWN_1.
PS51550	; EPH_LBD; 1.
PS50853	; FN3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00790	; RECEPTOR_TYR_KIN_V_1; 1.
PS00791	; RECEPTOR_TYR_KIN_V_2; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013