EKS-RAN-00006

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00006

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Eph	514.3	5.4E-155	602	861	260

Status

Reviewed

Ensembl Protein

ENSRNOP00000010634

UniProt Accession

P09759;

Protein Name

Ephrin type-B receptor 1

Protein Synonyms/Alias

ELK; Tyrosine-protein kinase receptor EPH-2;

Gene Name

Ephb1

Gene Synonyms/Alias

Ephb1; Elk, Epth2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000007865	ENSRNOP00000010634	ENSRNOT00000010634

Organism

Rattus norvegicus

Functional Description

Receptor tyrosine kinase which binds promiscuouslytransmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively (By similarity).

Protein Length

967

Protein Sequence
(FASTA)

MEETLMDTRT ATAELGWTAN PASGWEEVSG YDENLNTIRT YQVCNVFEPN QNNWLLTTFI 60

NRRGAHRIYT EMRFTVRDCS SLPNVPGSCK ETFNLYYYET DSVIATKKSA FWSEAPYLKV 120

DTIAADESFS QVDFGGRLMK VNTEVRSFGP LTRNGFYLAF QDYGACMSLL SVRVFFKKCP 180

SIVQNFAVFP ETMTGAESTS LVIARGTCIP NAEEVDVPIK LYCNGDGEWM VPIGRCTCKA 240

GYEPENSVAC KACPAGTFKA SQEAEGCSHC PSNSRSPSEA SPICTCRTGY YRADFDPPEV 300

ACTSVPSGPR NVISIVNETS IILEWHPPRE TGGRDDVTYN IICKKCRADR RSCSRCDDNV 360

EFVPRQLGLT ECRVSISSLW AHTPYTFDIQ AINGVSSKSP FPPQHVSVNI TTNQAAPSTV 420

PIMHQVSATM RSITLSWPQP EQPNGIILDY EIRYYEKEHN EFNSSMARSQ TNTARIDGLR 480

PGMVYVVQVR ARTVAGYGKF SGKMCFQTLT DDDYKSELRE QLPLIAGSAA AGVVFVVSLV 540

AISIVCSRKR AYSKEAVYSD KLQHYSTGRG SPGMKIYIDP FTYEDPNEAV REFAKEIDVS 600

FVKIEEVIGA GEFGEVYKGR LKLPGKREIY VAIKTLKAGY SEKQRRDFLS EASIMGQFDH 660

PNIIRLEGVV TKSRPVMIIT EFMENGALDS FLRQNDGQFT VIQLVGMLRG IAAGMKYLSE 720

MNYVHRDLAA RNILVNSNLV CKVSDFGLSR YLQDDTSDPT YTSSLGGKIP VRWTAPEAIA 780

YRKFTSASDV WSYGIVMWEV MSFGERPYWD MSNQDVINAI EQDYRLPPPM DCPAALHQLM 840

LDCWQKDRNS RPRFAEIVNT LDKMIRNPAS LKTVATITAV PSQPLLDRSI PDFTAFTTVD 900

DWLSAIKMVQ YRDSFLTAGF TSLQLVTQMT SEDLLRIGVT LAGHQKKILS SIHSMRVQMN 960

QSPSVMA 967

Nucleotide Sequence
(FASTA)

ATGGAAGAAA CATTGATGGA CACAAGGACT GCCACTGCAG AGTTGGGATG GACGGCCAAC 60

CCTGCCTCTG GGTGGGAAGA AGTCAGTGGC TACGATGAAA ACCTGAACAC CATCCGTACT 120

TACCAAGTGT GCAACGTCTT TGAACCCAAC CAGAACAACT GGCTGCTTAC CACCTTTATC 180

AACAGACGGG GCGCCCATCG CATCTATACA GAGATGCGCT TCACTGTGAG GGACTGCAGC 240

AGCCTTCCAA ATGTCCCAGG CTCCTGCAAA GAGACCTTCA ACTTATACTA CTATGAGACT 300

GACTCTGTCA TTGCCACCAA GAAGTCAGCC TTCTGGTCTG AAGCCCCCTA CCTCAAAGTT 360

GACACCATTG CTGCAGATGA GAGCTTCTCC CAGGTGGATT TTGGGGGAAG GTTGATGAAG 420

GTCAACACAG AAGTCAGGAG CTTTGGGCCT CTTACTAGGA ATGGTTTTTA CCTCGCTTTC 480

CAGGATTATG GAGCCTGTAT GTCTCTACTT TCTGTCCGTG TCTTCTTCAA AAAGTGTCCC 540

AGCATTGTGC AAAATTTTGC AGTGTTCCCA GAAACCATGA CAGGAGCAGA GAGCACATCT 600

CTGGTGATTG CTCGGGGCAC ATGCATCCCT AATGCAGAAG AAGTGGATGT GCCCATAAAA 660

CTCTACTGCA ATGGAGATGG AGAGTGGATG GTGCCCATTG GGCGCTGCAC CTGTAAGGCT 720

GGCTATGAAC CTGAGAACAG CGTGGCCTGC AAGGCCTGTC CTGCGGGAAC CTTCAAGGCC 780

AGCCAGGAAG CTGAAGGCTG CTCCCACTGC CCCTCCAACA GCCGCTCCCC TTCAGAGGCG 840

TCTCCCATCT GCACCTGCCG GACTGGCTAT TACCGAGCAG ACTTTGATCC CCCAGAAGTG 900

GCGTGCACTA GTGTCCCATC AGGCCCTCGA AATGTCATCT CCATTGTGAA TGAGACATCT 960

ATCATTCTAG AGTGGCACCC TCCAAGAGAG ACCGGTGGGC GAGATGATGT GACCTACAAC 1020

ATCATCTGCA AGAAGTGCAG AGCAGACCGC CGGAGTTGCT CCCGCTGCGA TGACAATGTG 1080

GAGTTTGTAC CCAGACAGCT GGGCTTGACT GAGTGTCGTG TCTCTATCAG TAGCCTATGG 1140

GCCCACACCC CATACACTTT TGATATCCAG GCCATCAATG GAGTCTCTAG CAAGAGTCCC 1200

TTCCCCCCAC AGCATGTCTC TGTCAACATC ACCACAAACC AAGCTGCCCC CTCCACTGTT 1260

CCTATCATGC ACCAGGTCAG TGCCACCATG AGGAGCATCA CCTTGTCATG GCCTCAGCCG 1320

GAGCAGCCTA ATGGCATCAT CCTGGACTAT GAGATCCGGT ACTATGAGAA GGAGCACAAT 1380

GAGTTCAACT CTTCCATGGC CAGGAGCCAG ACCAACACGG CACGTATCGA TGGGCTACGG 1440

CCCGGCATGG TGTATGTGGT CCAGGTACGA GCTCGAACCG TGGCTGGCTA TGGCAAGTTC 1500

AGTGGCAAGA TGTGCTTCCA GACTCTGACA GATGATGATT ACAAGTCGGA GCTGAGAGAG 1560

CAGCTACCCC TGATCGCTGG CTCTGCAGCA GCTGGGGTTG TATTTGTTGT GTCTCTGGTG 1620

GCTATCTCCA TTGTCTGCAG CAGGAAACGA GCTTACAGCA AAGAGGCTGT GTACAGTGAT 1680

AAACTTCAGC ATTACAGCAC AGGCCGAGGC TCCCCAGGGA TGAAGATCTA CATTGACCCG 1740

TTCACTTATG AGGACCCTAA TGAAGCTGTC CGGGAGTTTG CCAAGGAGAT TGATGTGTCT 1800

TTTGTGAAAA TTGAAGAGGT CATCGGAGCA GGGGAGTTTG GAGAAGTGTA CAAGGGCCGA 1860

TTGAAGCTGC CAGGCAAGAG GGAAATCTAT GTGGCCATCA AGACCCTGAA AGCTGGGTAC 1920

TCAGAGAAAC AGCGTCGGGA TTTTCTGAGC GAGGCGAGCA TCATGGGCCA GTTTGACCAT 1980

CCCAACATCA TTCGCCTAGA GGGTGTTGTC ACCAAGAGCC GACCTGTCAT GATCATTACG 2040

GAGTTCATGG AGAATGGCGC TTTAGACTCT TTCCTCCGGC AAAATGACGG ACAGTTCACC 2100

GTGATCCAGC TTGTGGGGAT GCTGAGGGGC ATCGCTGCTG GCATGAAGTA CCTATCTGAG 2160

ATGAATTATG TGCACCGGGA CCTGGCTGCT AGGAACATTC TGGTGAACAG CAACCTGGTG 2220

TGCAAAGTGT CTGACTTTGG CCTCTCTCGC TACCTCCAGG ATGACACCTC AGACCCCACC 2280

TACACCAGCT CCTTGGGAGG GAAGATACCT GTTAGATGGA CAGCTCCAGA GGCCATCGCC 2340

TACCGCAAGT TTACGTCAGC CAGCGATGTC TGGAGCTACG GGATTGTCAT GTGGGAAGTA 2400

ATGTCATTTG GAGAGAGACC TTACTGGGAT ATGTCCAATC AAGATGTCAT CAATGCCATT 2460

GAGCAGGACT ACCGGCTGCC CCCTCCTATG GACTGCCCAG CTGCCCTGCA CCAGCTCATG 2520

CTGGACTGTT GGCAGAAGGA TCGAAATAGC CGGCCCCGTT TTGCAGAGAT CGTCAACACC 2580

CTGGACAAGA TGATCCGGAA CCCAGCTAGT CTCAAGACTG TGGCAACCAT CACCGCTGTG 2640

CCTTCCCAAC CCCTGCTTGA CCGCTCTATC CCAGACTTCA CGGCCTTTAC CACCGTGGAT 2700

GACTGGCTAA GTGCCATCAA AATGGTCCAG TACAGGGACA GCTTCCTCAC CGCTGGCTTC 2760

ACCTCCCTCC AGCTGGTCAC CCAGATGACA TCAGAAGACC TCCTGAGAAT AGGGGTCACC 2820

TTGGCAGGCC ATCAGAAGAA GATTCTTAGC AGCATCCACT CGATGAGGGT CCAGATGAAC 2880

CAGTCACCGT CGGTAATGGC ATGA 2904

Domain Profile

S: 1     vkiekviGaGefGevcsGrlklpgkreiavaiktlkagstekqrrdflseasilGqfdhp  60

         vkie+viGaGefGev++Grlklpgkrei+vaiktlkag++ekqrrdflseasi+Gqfdhp

Q: 602   VKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHP  661

         79**********************************************************

S: 61    niirleGvvtkskpvmivteymengaldsflrkkdgqftviqlvgmlrGiasgmkylsdm  120

         niirleGvvtks+pvmi+te+mengaldsflr++dgqftviqlvgmlrGia+gmkyls+m

Q: 662   NIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSEM  721

         ************************************************************

S: 121   gyvhrdlaarnilvnsnlvckvsdfGlsrvledds.eaaytts.ggkipirwtapeaiay  178

         +yvhrdlaarnilvnsnlvckvsdfGlsr l+dd+ +++yt+s ggkip+rwtapeaiay

Q: 722   NYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTsDPTYTSSlGGKIPVRWTAPEAIAY  781

         *******************************99999*****999****************

S: 179   rkftsasdvwsyGivlwevlsyGerpywdmsnqdvikaieegyrlpppmdcpaalyqlml  238

         rkftsasdvwsyGiv+wev+s+Gerpywdmsnqdvi+aie++yrlpppmdcpaal+qlml

Q: 782   RKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLML  841

         ************************************************************

S: 239   dcwqkdraerpkfeqivsil  258

         dcwqkdr++rp+f++iv++l

Q: 842   DCWQKDRNSRPRFAEIVNTL  861

         *****************975

Domain Sequence
(FASTA)

VKIEEVIGAG EFGEVYKGRL KLPGKREIYV AIKTLKAGYS EKQRRDFLSE ASIMGQFDHP 60

NIIRLEGVVT KSRPVMIITE FMENGALDSF LRQNDGQFTV IQLVGMLRGI AAGMKYLSEM 120

NYVHRDLAAR NILVNSNLVC KVSDFGLSRY LQDDTSDPTY TSSLGGKIPV RWTAPEAIAY 180

RKFTSASDVW SYGIVMWEVM SFGERPYWDM SNQDVINAIE QDYRLPPPMD CPAALHQLML 240

DCWQKDRNSR PRFAEIVNTL 260

Keyword

ATP-binding; Cell adhesion; Cell membrane; Cell projection; Complete proteome; Endosome; Glycoprotein; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0030424	; C:axon
GO:0030425	; C:dendrite
GO:0031901	; C:early endosome membrane
GO:0005887	; C:integral to plasma membrane
GO:0045121	; C:membrane raft
GO:0005524	; F:ATP binding
GO:0008046	; F:axon guidance receptor activity
GO:0005005	; F:transmembrane-ephrin receptor activity
GO:0001525	; P:angiogenesis
GO:0048593	; P:camera-type eye morphogenesis
GO:0060326	; P:cell chemotaxis
GO:0031589	; P:cell-substrate adhesion
GO:0021952	; P:central nervous system projection neuron axonogenesis
GO:0060997	; P:dendritic spine morphogenesis
GO:0050965	; P:detection of temperature stimulus involved in sensory perception of pain
GO:0030010	; P:establishment of cell polarity
GO:0061351	; P:neural precursor cell proliferation
GO:0021631	; P:optic nerve morphogenesis
GO:0051965	; P:positive regulation of synapse assembly
GO:0046777	; P:protein autophosphorylation
GO:0070372	; P:regulation of ERK1 and ERK2 cascade
GO:0046328	; P:regulation of JNK cascade
GO:0031290	; P:retinal ganglion cell axon guidance

KEGG

rno:24338

;

InterPros

IPR001090	; Ephrin_rcpt_lig-bd_dom.
IPR003961	; Fibronectin_type3.
IPR008979	; Galactose-bd-like.
IPR013783	; Ig-like_fold.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR021129	; SAM_type1.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016257	; Tyr_kinase_ephrin_rcpt.
IPR001426	; Tyr_kinase_rcpt_V_CS.

Pfam

PF01404	; Ephrin_lbd; 1.
PF00041	; fn3; 2.
PF07714	; Pkinase_Tyr; 1.
PF00536	; SAM_1; 1.

SMARTs

SM00615	; EPH_lbd; 1.
SM00060	; FN3; 2.
SM00454	; SAM; 1.
SM00219	; TyrKc; 1.

Prosites

PS01186	; EGF_2; UNKNOWN_1.
PS51550	; EPH_LBD; 1.
PS50853	; FN3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00790	; RECEPTOR_TYR_KIN_V_1; 1.
PS00791	; RECEPTOR_TYR_KIN_V_2; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013