EKS-RAN-00169

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00169

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	443.4	3.6E-133	254	522	269

Status

Reviewed

Ensembl Protein

ENSRNOP00000012924

UniProt Accession

F1M7Y5; B1PZT8;

Protein Name

Protein kinase C iota type

Protein Synonyms/Alias

Atypical protein kinase C-lambda/iota; aPKC-lambda/iota; nPKC-iota;

Gene Name

Prkci

Gene Synonyms/Alias

Prkci; Pkcl;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000009652	ENSRNOP00000012924	ENSRNOT00000012924

Organism

Rattus norvegicus

Functional Description

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non- small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Downstream of PI3K is required for insulin-stimulated glucose transport. Activates RAB4A and promotes its associtation with KIF3A which is required for the insulin- induced SLC2A4/GLUT4 translocation in adipocytes. Is essential in early embryogenesis and development of differentiating photoreceptors by playing a role in the establishment of epithelial and neuronal polarity (By similarity).

Protein Length

596

Protein Sequence
(FASTA)

MPTQRDSSTM SHTVACGGGG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CSEVRDMCSF 60

DNEQPFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE 120

DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK 180

KCHKLVTIEC GRHSLPPEPM MPMDQSSMHP DHTQTVIPYN PSSHESLDQV GEEKEAMNTR 240

ESGKASSSLG LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ 300

TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL PEEHARFYSA 360

EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI 420

APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR 480

IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK 540

PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV 596

Nucleotide Sequence
(FASTA)

ATGCCGACCC AGAGGGACAG CAGCACCATG TCTCACACGG TCGCGTGCGG CGGCGGCGGG 60

GACCATTCCC ACCAGGTCCG GGTGAAAGCC TACTACCGCG GGGATATTAT GATAACACAC 120

TTCGAGCCTT CCATCTCCTT TGAAGGACTT TGCAGTGAGG TTCGAGATAT GTGTTCTTTT 180

GACAATGAGC AGCCATTCAC CATGAAATGG ATAGATGAGG AAGGAGACCC GTGCACAGTG 240

TCTTCTCAGT TGGAGTTGGA AGAGGCTTTC AGGCTGTATG AGTTGAACAA GGATTCTGAA 300

CTCCTGATCC ACGTGTTCCC GTGTGTACCA GAGCGTCCTG GAATGCCTTG CCCAGGGGAA 360

GACAAGTCCA TTTACCGCAG AGGGGCGCGC AGGTGGAGAA AGCTGTATTG TGCAAATGGC 420

CACACTTTTC AAGCCAAACG CTTTAACAGG CGTGCCCACT GTGCCATCTG CACAGACAGG 480

ATCTGGGGAC TTGGACGACA AGGATATAAA TGCATCAACT GCAAACTGCT GGTTCATAAG 540

AAGTGCCACA AGCTTGTCAC AATTGAGTGT GGGCGGCATT CTTTGCCACC GGAACCCATG 600

ATGCCAATGG ACCAGTCATC CATGCACCCA GACCACACAC AGACAGTAAT TCCATATAAT 660

CCTTCAAGTC ATGAGAGTTT GGACCAAGTT GGTGAAGAAA AGGAGGCAAT GAACACCAGG 720

GAGAGTGGGA AGGCGTCATC AAGCTTAGGT CTCCAGGATT TCGATTTGCT TCGAGTTATA 780

GGGAGAGGAA GTTACGCCAA AGTACTGCTG GTTCGATTAA AAAAGACAGA TCGCATTTAT 840

GCAATGAAGG TTGTGAAGAA AGAGCTCGTC AATGACGATG AGGATATTGA TTGGGTACAG 900

ACAGAAAAGC ATGTGTTTGA GCAGGCGTCC AATCACCCTT TCCTTGTTGG TCTGCATTCC 960

TGCTTCCAGA CAGAAAGCAG GCTGTTTTTT GTCATAGAAT ATGTGAATGG AGGGGATCTC 1020

ATGTTTCATA TGCAGCGGCA AAGAAAACTT CCTGAAGAAC ATGCCAGGTT TTACTCAGCA 1080

GAAATCAGTC TAGCACTAAA TTATCTTCAT GAGCGAGGGA TAATTTATAG AGATTTGAAG 1140

TTGGACAATG TACTGCTGGA CTCTGAAGGA CACATCAAAC TCACTGACTA CGGCATGTGT 1200

AAGGAAGGAT TACGGCCCGG AGATACAACC AGCACCTTCT GTGGGACTCC CAATTACATT 1260

GCTCCTGAGA TCTTAAGAGG AGAAGACTAT GGCTTCAGCG TTGACTGGTG GGCTCTTGGA 1320

GTACTCATGT TTGAGATGAT GGCGGGAAGG TCTCCATTTG ATATCGTTGG GAGCTCTGAC 1380

AATCCTGACC AAAACACAGA GGATTATCTG TTCCAAGTCA TTTTGGAGAA GCAGATTCGC 1440

ATACCGCGCT CCCTGTCTGT GAAAGCAGCA AGTGTGCTGA AGAGTTTCCT CAACAAGGAC 1500

CCAAAGGAAC GATTGGGTTG TCACCCTCAA ACTGGATTTG CTGACATCCA AGGACACCCA 1560

TTCTTCCGTA ATGTGGATTG GGACATGATG GAGCAGAAGC AAGTGGTTCC GCCCTTTAAA 1620

CCAAACATTT CTGGAGAATT TGGTTTGGAT AACTTTGACT CCCAGTTTAC CAACGAACCA 1680

GTCCAGCTCA CTCCAGATGA TGATGACATC GTGAGGAAGA TTGATCAGTC TGAATTTGAA 1740

GGTTTCGAGT ATATCAACCC TCTCTTGATG TCTGCAGAAG AGTGTGTCTG A 1791

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp  60

         f+ll+v+G+Gs++kvll++lkktd++ya+kv+kk++v++d+d++++++ek+v+++as++p

Q: 254   FDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHP  313

         89**********************************************************

S: 61    flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg  120

         flv l+scfqte+rlffv+eyvnGGdlmfh+q++rkl+ee+arfy+aei+lal++lhe+g

Q: 314   FLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERG  373

         ************************************************************

S: 121   iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks  180

         iiyrdlkldnvlld+eGhikl+d+G+cke++  g+ttstfcGtP+yiaPeil++e+yg+s

Q: 374   IIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFS  433

         ************************************************************

S: 181   vdwwalGvllyemlagqsPfe.........gededelfesilekevlyPkslskeaveil  231

         vdwwalGvl++em+ag+sPf+         +++ed+lf++ilek++++P+sls++a+++l

Q: 434   VDWWALGVLMFEMMAGRSPFDivgssdnpdQNTEDYLFQVILEKQIRIPRSLSVKAASVL  493

         *****************************99*****************************

S: 232   kglltkdpekrlGvk.eegeedikehaff  259

         k++l+kdp++rlG++ ++g +di+ h+ff

Q: 494   KSFLNKDPKERLGCHpQTGFADIQGHPFF  522

         **************989***********9

Domain Sequence
(FASTA)

FDLLRVIGRG SYAKVLLVRL KKTDRIYAMK VVKKELVNDD EDIDWVQTEK HVFEQASNHP 60

FLVGLHSCFQ TESRLFFVIE YVNGGDLMFH MQRQRKLPEE HARFYSAEIS LALNYLHERG 120

IIYRDLKLDN VLLDSEGHIK LTDYGMCKEG LRPGDTTSTF CGTPNYIAPE ILRGEDYGFS 180

VDWWALGVLM FEMMAGRSPF DIVGSSDNPD QNTEDYLFQV ILEKQIRIPR SLSVKAASVL 240

KSFLNKDPKE RLGCHPQTGF ADIQGHPFF 269

Keyword

ATP-binding; Complete proteome; Cytoplasm; Developmental protein; Endosome; Kinase; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0031252	; C:cell leading edge
GO:0005829	; C:cytosol
GO:0005768	; C:endosome
GO:0005634	; C:nucleus
GO:0005886	; C:plasma membrane
GO:0043234	; C:protein complex
GO:0005524	; F:ATP binding
GO:0004697	; F:protein kinase C activity
GO:0008270	; F:zinc ion binding
GO:0016477	; P:cell migration
GO:0034613	; P:cellular protein localization
GO:0032869	; P:cellular response to insulin stimulus
GO:0048194	; P:Golgi vesicle budding
GO:0035556	; P:intracellular signal transduction
GO:0034351	; P:negative regulation of glial cell apoptotic process
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:2000353	; P:positive regulation of endothelial cell apoptotic process
GO:0060252	; P:positive regulation of glial cell proliferation
GO:0010976	; P:positive regulation of neuron projection development
GO:0051092	; P:positive regulation of NF-kappaB transcription factor activity
GO:0070555	; P:response to interleukin-1

KEGG

rno:84006

;

InterPros

IPR000961	; AGC-kinase_C.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR000270	; OPR_PB1.
IPR012233	; PKC_zeta.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF00564	; PB1; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 1.
SM00666	; PB1; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013