| Tag | Content |
|---|
| EKPD ID | EKS-RAN-00384 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| TK/Src | 432.7 | 5.0E-130 | 245 | 494 | 250 |
|
| Status | Reviewed |
| Ensembl Protein | ENSRNOP00000012936 |
| UniProt Accession | Q01621; B1H265; |
| Protein Name | Proto-oncogene tyrosine-protein kinase LCK |
| Protein Synonyms/Alias | Lymphocyte cell-specific protein-tyrosine kinase; p56-LCK; |
| Gene Name | Lck |
| Gene Synonyms/Alias | Lck; |
| Ensembl Information | |
| Organism | Rattus norvegicus |
| Functional Description | Non-receptor tyrosine-protein kinase that plays anessential role in the selection and maturation of developing T- cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP (By similarity). |
| Protein Length | 509 |
Protein Sequence
(FASTA) | | MGCVCSSNPE DDWMENIDVC ENCHYPIVPL DSKSTLPIRT GSEVRDPLVT YEGSLPPASP 60 | | LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA QSLTTGQEGF IPFNFVAKAN 120 | | SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS FLIRESESTA GSFSLSVRDF DQNQGEVVKH 180 | | YKIRNLDNGG FYISPRITFP GLHDLVRHYT NASDGLCTKL SRPCQTQKPQ KPWWEDEWEV 240 | | PRETLKLVER LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHPRL 300 | | VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLNVNK LLDMAAQIAE GMAFIEEQNY 360 | | IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA KFPIKWTAPE AINYGTFTIK 420 | | SDVWSFGILL TEIVTHGRIP YPGMTNPEVI QNLEKGYRMV RPDNCPEELY HLMMLCWKER 480 | | PEDRPTFDYL RSVLDDFFTA TEGQYQPQP 509 |
|
Nucleotide Sequence
(FASTA) | | ATGGGCTGTG TCTGCAGCTC AAACCCCGAA GATGACTGGA TGGAGAACAT TGACGTGTGT 60 | | GAAAACTGCC ACTATCCCAT AGTCCCACTG GACAGCAAGA GTACGCTGCC CATCCGGACC 120 | | GGCTCTGAAG TTCGGGACCC ACTGGTCACC TATGAGGGCT CCCTCCCACC AGCCTCCCCG 180 | | CTGCAAGACA ACCTAGTTAT TGCCCTGCAC AGCTATGAGC CCTCCCATGA TGGAGACTTG 240 | | GGCTTTGAAA AGGGTGAACA GCTCCGAATC CTGGAGCAGA GCGGTGAGTG GTGGAAGGCT 300 | | CAGTCCCTGA CCACTGGCCA AGAAGGCTTC ATCCCCTTCA ACTTCGTGGC GAAAGCAAAC 360 | | AGCCTGGAGC CTGAACCTTG GTTCTTCAAA AATCTGAGCC GTAAGGACGC CGAGCGGCAG 420 | | CTTTTGGCGC CCGGGAACAC GCATGGATCC TTCCTGATCC GGGAAAGCGA AAGCACCGCC 480 | | GGATCCTTTT CACTGTCAGT CAGAGACTTC GACCAGAACC AGGGAGAAGT GGTGAAACAT 540 | | TACAAGATCC GTAACCTAGA CAACGGTGGC TTCTACATCT CCCCTCGTAT CACTTTTCCC 600 | | GGACTACACG ATCTGGTCCG CCATTACACC AACGCCTCTG ATGGGCTGTG CACAAAATTG 660 | | AGCCGTCCTT GCCAGACCCA GAAGCCCCAG AAACCATGGT GGGAGGACGA ATGGGAAGTT 720 | | CCCAGAGAGA CATTGAAGTT GGTTGAGCGG CTGGGAGCTG GCCAGTTCGG GGAAGTGTGG 780 | | ATGGGATACT ACAATGGACA CACGAAGGTG GCAGTGAAAA GTCTGAAGCA AGGGAGTATG 840 | | TCTCCTGATG CCTTCCTGGC AGAGGCTAAC CTCATGAAGC AGCTGCAGCA CCCGCGGCTC 900 | | GTCCGGCTTT ATGCAGTGGT CACCCAGGAG CCCATCTACA TTATCACAGA ATACATGGAG 960 | | AACGGGAGCC TAGTAGATTT CCTCAAGACT CCCTCGGGCA TCAAGTTGAA CGTCAACAAA 1020 | | CTTTTGGACA TGGCAGCCCA GATTGCAGAG GGCATGGCGT TCATCGAAGA GCAGAATTAC 1080 | | ATCCATCGTG ACCTGCGGGC TGCCAACATC CTGGTGTCTG ACACGCTGAG CTGCAAAATT 1140 | | GCAGACTTTG GCCTGGCACG TCTGATTGAG GACAATGAAT ACACGGCCCG GGAGGGGGCC 1200 | | AAGTTTCCCA TTAAGTGGAC AGCACCAGAA GCCATTAACT ATGGGACCTT TACCATCAAG 1260 | | TCAGATGTGT GGTCCTTCGG GATCCTGCTG ACAGAGATCG TCACCCATGG CCGGATCCCT 1320 | | TACCCAGGAA TGACCAACCC TGAAGTCATT CAGAACCTGG AAAAAGGCTA CCGAATGGTG 1380 | | CGACCTGACA ACTGCCCGGA AGAGCTGTAC CACCTCATGA TGCTGTGCTG GAAGGAGCGC 1440 | | CCAGAGGACA GGCCCACGTT TGACTACCTT CGGAGCGTTC TGGATGACTT CTTCACAGCC 1500 | | ACAGAGGGCC AGTACCAGCC CCAGCCTTGA 1530 |
|
| Domain Profile | S: 1 lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly 60 | lklv++lG+GqfGevw+g+++g++kvavk+lk+g++sp+afl+ea+++k+l+h++lv+ly | Q: 245 LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLY 304 | 6899******************************************************** |
| S: 61 avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr 120 | avv++ ePiyi+te+m++Gsl+dflk+ +g kl+++kl+d+aaq+aeGma++ee+n+ihr | Q: 305 AVVTQ-EPIYIITEYMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHR 363 | ****9.****************************************************** |
| S: 121 dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv 180 | dl+a+n+lv+++l +k+adfGlarlied+eyta+egak+PikWtaPeai++g+ftiksdv | Q: 364 DLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDV 423 | ************************************************************ |
| S: 181 WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee 240 | WsfGillteivt+G++PypGmtn ev++++e+gyr+ rp++cpeely+l++ cwk++pe+ | Q: 424 WSFGILLTEIVTHGRIPYPGMTNPEVIQNLEKGYRMVRPDNCPEELYHLMMLCWKERPED 483 | ************************************************************ |
| S: 241 rPtfetlqevl 251 | rPtf++l++vl | Q: 484 RPTFDYLRSVL 494 | *******9876 |
|
Domain Sequence
(FASTA) | | LKLVERLGAG QFGEVWMGYY NGHTKVAVKS LKQGSMSPDA FLAEANLMKQ LQHPRLVRLY 60 | | AVVTQEPIYI ITEYMENGSL VDFLKTPSGI KLNVNKLLDM AAQIAEGMAF IEEQNYIHRD 120 | | LRAANILVSD TLSCKIADFG LARLIEDNEY TAREGAKFPI KWTAPEAINY GTFTIKSDVW 180 | | SFGILLTEIV THGRIPYPGM TNPEVIQNLE KGYRMVRPDN CPEELYHLMM LCWKERPEDR 240 | | PTFDYLRSVL 250 |
|
| Keyword | Acetylation; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Proto-oncogene; Reference proteome; SH3 domain; Transferase; Tyrosine-protein kinase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
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| Prints | |
| Created Date | 20-Feb-2013 |