EKS-RAN-00437

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00437

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	375.7	1.3E-112	298	568	271

Status

Reviewed

Ensembl Protein

ENSRNOP00000013362

UniProt Accession

Q63802; Q5EAN3;

Protein Name

Wee1-like protein kinase

Protein Synonyms/Alias

Wee1A kinase;

Gene Name

Wee1

Gene Synonyms/Alias

Wee1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000010017	ENSRNOP00000013362	ENSRNOT00000013362

Organism

Rattus norvegicus

Functional Description

Acts as a negative regulator of entry into mitosis (G2to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).

Protein Length

646

Protein Sequence
(FASTA)

MSFLSRQQPP PTRRAAAACS LRQKLIFSPG SDCEEEEEEE EEGSGHSTGE DSAFQEPDSP 60

LPSARSPAEA EAERRRRSPG AEPSSPGELE EDLLLRGGGG GAQAAGGGAE GDSWEEEGFG 120

SSSPVKSPTT AYFLGSSFSP VRCGGPGDAS PRGCGVPRAM DDPCSPQPDY PSTPPHKTFR 180

KLRLFDTPHT PKSLLSKARV IDSSSVKLRG SSLFMDTEKS GKREFDTRQT PQVNINPFTP 240

DPVMLHSSGQ CRGRKRAYFN DSSEDMEASD YEFEDETRPA KRITITESNM KSRYTTEFHE 300

LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA VLGQHPHVVR 360

YFSAWAEDDH MLIQNEYCNG GSLADAVSEN YRVMSYFTEA ELKDLLLQVG RGLRYIHSMS 420

LVHMDIKPSN IFISRTSIPN AVSEEGDEDD WISNKVMFKI GDLGHVTRIS SPQVEEGDSR 480

FLANEVLQEN YSHLPKADIF ALALTVVCAA GAEPLPRNGD QWHEIRQGRL PRIPQVLSQE 540

LTELLKVMIH PDPERRPSAM VLVKHSVLLS ASRKSAEQLR IELNAEKFKN SLLQKELKKA 600

QMAAKVAAEE RALLTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY 646

Nucleotide Sequence
(FASTA)

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGCCGCAGC CGCCTGCAGT 60

TTGCGGCAGA AGCTCATCTT CTCACCCGGC AGCGATTGTG AGGAGGAAGA GGAGGAGGAG 120

GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAGCC TGATTCGCCG 180

CTGCCCTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGCCG CTCGCCCGGC 240

GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GAGGACTTGC TGCTGCGGGG CGGCGGCGGG 300

GGCGCGCAAG CGGCGGGCGG AGGAGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 360

TCTTCGTCGC CCGTCAAGTC ACCTACGACT GCCTACTTTC TGGGCAGCTC GTTCTCGCCG 420

GTGCGCTGCG GCGGCCCTGG GGATGCCTCC CCGCGGGGCT GCGGGGTCCC GCGAGCCATG 480

GACGACCCCT GTTCTCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 540

AAGTTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCTCGAGTT 600

ATTGATTCCA GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC TGAAAAATCA 660

GGAAAAAGAG AATTCGACAC ACGGCAAACT CCTCAAGTGA ATATTAACCC CTTTACTCCG 720

GATCCTGTAA TGCTTCATTC CTCAGGACAG TGTCGTGGGA GAAAGAGAGC ATATTTTAAT 780

GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 840

AAAAGAATTA CAATTACTGA AAGCAATATG AAGTCACGAT ATACAACTGA ATTTCATGAG 900

CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGCTAGAT 960

GGATGCATTT ATGCCATTAA ACGATCAAAA AAGCCATTGG CTGGCTCTGT TGATGAACAG 1020

AATGCTTTGA GAGAAGTGTA TGCTCATGCT GTGCTTGGAC AGCATCCCCA TGTGGTTCGT 1080

TATTTCTCTG CCTGGGCAGA AGATGACCAT ATGCTTATAC AGAATGAATA CTGTAATGGT 1140

GGGAGTTTAG CTGACGCTGT AAGTGAGAAC TACAGAGTCA TGAGCTACTT TACTGAAGCA 1200

GAGCTGAAGG ATCTCCTTTT GCAAGTTGGC AGGGGCTTGA GATACATACA TTCAATGTCT 1260

TTGGTTCACA TGGATATAAA ACCTAGTAAT ATTTTTATAT CTCGAACCTC AATCCCAAAT 1320

GCAGTCTCTG AAGAAGGAGA TGAAGATGAC TGGATATCCA ACAAAGTTAT GTTTAAAATA 1380

GGTGATCTTG GGCATGTAAC AAGAATCTCT AGTCCACAAG TTGAAGAAGG TGATAGTCGT 1440

TTTCTAGCAA ATGAAGTTTT ACAAGAGAAC TATAGCCATC TACCGAAAGC AGATATTTTT 1500

GCTCTTGCTC TCACAGTCGT ATGTGCTGCT GGTGCTGAAC CCCTTCCCAG AAATGGAGAC 1560

CAGTGGCATG AAATCCGACA GGGTCGATTA CCTCGGATTC CACAAGTGCT TTCCCAGGAG 1620

TTGACAGAGT TGCTAAAAGT TATGATTCAT CCTGATCCAG AGAGAAGACC TTCAGCAATG 1680

GTGCTGGTGA AGCATTCAGT GTTGCTGTCT GCCTCTAGAA AGAGCGCAGA GCAGTTACGA 1740

ATAGAATTGA ATGCTGAAAA ATTCAAAAAT TCTCTTTTGC AGAAAGAACT CAAGAAAGCC 1800

CAAATGGCAG CCAAAGTTGC AGCTGAGGAA CGGGCACTCC TCACAGATCG GATGGCCACT 1860

AGATCTACCA CCCAGAGTAA TAGAACTTCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 1920

GTCAGCCTTA CTATATACTG A 1941

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikkskkplkglskekkalrEvtahavlgqhen  60

         F+e+ekiG+Gefg+vfk+vkr+dg++yAik+skkpl+g+++e++alrEv+ahavlgqh++

Q: 298   FHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPH  357

         99**********************************************************

S: 61    vvryisaWeeddslyiqtElCeggsLeaalqeceakkkaleeaelkkilldlllglrylH  120

         vvry+saW+edd+++iq+E+C+ggsL++a++e+++++++++eaelk++ll++ +glry+H

Q: 358   VVRYFSAWAEDDHMLIQNEYCNGGSLADAVSENYRVMSYFTEAELKDLLLQVGRGLRYIH  417

         ************************************************************

S: 121   ssslvHlDikpenifi...................tkevtlklgDlGlvtelnsedkskv  161

         s+slvH+Dikp+nifi                   +++v++k+gDlG+vt++   ++++v

Q: 418   SMSLVHMDIKPSNIFIsrtsipnavseegdeddwiSNKVMFKIGDLGHVTRI---SSPQV  474

         ****************************************************...9****

S: 162   eEGDsrylakEilqeeyskl.kaDiFslgltilevaanvelPsnGeaWhklRkgklpdei  220

         eEGDsr+la+E+lqe+ys+l kaDiF+l+lt++++a++++lP+nG++Wh++R+g+lp +i

Q: 475   EEGDSRFLANEVLQENYSHLpKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLP-RI  533

         *********************************************************.**

S: 221   lkelsedlrellklmlepdperRptaeellkhevl  255

          ++ls++l+ellk+m++pdperRp+a+ l+kh+vl

Q: 534   PQVLSQELTELLKVMIHPDPERRPSAMVLVKHSVL  568

         ********************************986

Domain Sequence
(FASTA)

FHELEKIGSG EFGSVFKCVK RLDGCIYAIK RSKKPLAGSV DEQNALREVY AHAVLGQHPH 60

VVRYFSAWAE DDHMLIQNEY CNGGSLADAV SENYRVMSYF TEAELKDLLL QVGRGLRYIH 120

SMSLVHMDIK PSNIFISRTS IPNAVSEEGD EDDWISNKVM FKIGDLGHVT RISSPQVEEG 180

DSRFLANEVL QENYSHLPKA DIFALALTVV CAAGAEPLPR NGDQWHEIRQ GRLPRIPQVL 240

SQELTELLKV MIHPDPERRP SAMVLVKHSV L 271

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0030010	; P:establishment of cell polarity
GO:0000226	; P:microtubule cytoskeleton organization
GO:0007067	; P:mitosis
GO:0048812	; P:neuron projection morphogenesis
GO:0018108	; P:peptidyl-tyrosine phosphorylation

KEGG

rno:308937

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR017164	; Wee1-like_protein_kinase.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013