EKS-RAN-00278

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00278

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	388.4	2.0E-116	12	265	254

Status

Reviewed

Ensembl Protein

ENSRNOP00000016946

UniProt Accession

B2GUY1;

Protein Name

Serine/threonine-protein kinase PLK4

Protein Synonyms/Alias

Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase Sak;

Gene Name

Plk4

Gene Synonyms/Alias

Plk4; Sak;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000011654	ENSRNOP00000016946	ENSRNOT00000016946

Organism

Rattus norvegicus

Functional Description

Serine/threonine-protein kinase that plays a centralrole in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/ (By similarity).

Protein Length

924

Protein Sequence
(FASTA)

MAACIGERIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE 60

VKIHCQLKHP SVLELYNYFE DNNYVYLVLE MCHNGEMNRY LKNRMKPFSE SEARHFMHQI 120

ITGMLYLHSH GILHRDLTLS NILLTRNMNI KIADFGLATQ LKMPHEKHYT LCGTPNYISP 180

EIATRSAHGL ESDIWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PAFLSREAQD 240

LIHQLLRRNP ADRLSLSSVL DHPFMSRNPS TKSKDLGTVE DSMDSGHATL STTITASSGT 300

SLSGSLLDRR RLLVGQPLPN KITVFQKNKN SSDFSSGDGS NFCTQWGNPE QEANNRGRGR 360

VIEDAEERPH SRYLRRAHSS DRSNPSNQSR AKTYSIERCH SVEMLSKPRR SSLDETKHSS 420

NHHCLGKTPF PFADQTPQME IVQQWFGNLQ MNGETSEHNT ISPNRDFQDY PDVQDTLRNT 480

WTDTRASKNS DNSANVHPAK QLSTMKYMTA HHHKPEIMQQ ELAIHPHSEQ NKSRSMESTL 540

GYRKPTLRSI TSPLVAHRLK PIRQKTKKAV VSILDSEEVC VELLKECTSE GHVKEVLQIS 600

SDGTTITVYY PNDGRGFPLA DRPPLPTDNI SRYSFDSLPE KYWRKYQYAS RFIQLVRSKT 660

PKITYFTRYA KCILMENSPG ADFEVWFYDG AKIHKTEDVI HIIEKTGLSY TLKNENDFTS 720

LKEEVKIYMD HANEGHRTCL ALESVISEEE KRSRGSSFFP IIVGRKPGTT SSPKALSPPP 780

VDPGYSKGEQ ASSSRLSANS AAFPTQTPVL SPSAVTVEGP GQTAATTGTS ISSSLPKSAQ 840

LLKSVFVKNV GWATQLTSGA VWVQFNDGSQ LVVQAGVSSI SYTSPDGQTT RYGENEKLPE 900

YIKQKLQCLS SILLMFSNPT PSFQ 924

Nucleotide Sequence
(FASTA)

ATGGCGGCGT GCATCGGAGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTTGGTAAA 60

GGATCATTTG CTGGTGTCTA CAGAGCTGAG TCCATACACA CTGGTTTGGA AGTTGCAATC 120

AAAATGATAG ATAAGAAAGC CATGTACAAA GCTGGAATGG TACAGAGAGT CCAAAATGAG 180

GTGAAAATAC ATTGCCAGTT GAAACACCCC TCTGTCTTGG AGCTCTATAA TTACTTTGAA 240

GATAACAATT ACGTATACCT GGTATTAGAA ATGTGCCACA ACGGAGAAAT GAACAGGTAC 300

CTAAAGAACA GAATGAAGCC TTTCTCCGAA AGCGAAGCCA GGCACTTCAT GCACCAGATC 360

ATCACAGGAA TGCTGTATCT TCATTCTCAC GGCATACTAC ACCGCGACCT CACACTCTCT 420

AACATCTTAC TTACACGGAA TATGAACATA AAAATTGCTG ACTTTGGACT AGCAACACAA 480

TTGAAAATGC CACATGAGAA GCATTACACC CTGTGTGGAA CCCCTAATTA TATTTCACCA 540

GAAATTGCAA CTCGAAGTGC ACATGGACTT GAATCTGATA TTTGGTCATT GGGCTGTATG 600

TTTTATACAT TACTTATTGG AAGACCACCT TTTGACACTG ATACAGTCAA AAACACATTG 660

AACAAAGTAG TATTGGCAGA TTATGAAATG CCAGCCTTTT TGTCACGAGA GGCCCAGGAC 720

CTTATCCACC AATTACTTCG TAGAAACCCT GCAGATCGGT TAAGTCTGTC TTCTGTGTTG 780

GACCATCCTT TTATGTCACG AAATCCTTCA ACAAAGAGTA AAGACTTAGG GACTGTAGAG 840

GACTCAATGG ATAGTGGGCA TGCCACACTT TCCACAACAA TTACAGCTTC TTCCGGAACC 900

AGTTTGAGTG GTAGCCTACT TGACAGAAGA AGACTTTTGG TTGGTCAACC ACTTCCAAAT 960

AAAATTACTG TATTTCAAAA AAATAAAAAT TCAAGTGACT TTTCTTCAGG AGATGGAAGT 1020

AATTTTTGTA CTCAATGGGG AAATCCAGAA CAAGAAGCTA ATAATAGGGG GCGGGGAAGA 1080

GTAATTGAAG ATGCAGAAGA AAGGCCACAT TCTCGATACC TTCGCAGAGC TCATTCCTCT 1140

GATAGATCCA ACCCTTCTAA TCAGTCTCGA GCAAAAACAT ACTCAATAGA GCGGTGTCAC 1200

TCAGTAGAAA TGCTTTCAAA GCCCAGAAGA TCATCACTGG ATGAAACTAA ACACAGTTCC 1260

AATCATCATT GTCTAGGGAA AACTCCTTTT CCATTTGCAG ACCAGACACC TCAGATGGAG 1320

ATAGTACAAC AGTGGTTTGG GAATCTGCAA ATGAATGGAG AAACTAGTGA GCACAACACC 1380

ATTAGCCCAA ACAGAGATTT CCAGGACTAT CCTGATGTGC AGGACACATT AAGAAATACT 1440

TGGACTGACA CAAGAGCCAG CAAGAATTCC GATAATTCTG CTAACGTTCA TCCTGCAAAG 1500

CAGCTGAGTA CCATGAAATA CATGACTGCA CATCACCATA AACCTGAGAT CATGCAGCAA 1560

GAGCTGGCCA TACATCCTCA TTCTGAACAA AACAAGAGTA GAAGTATGGA GTCAACATTG 1620

GGTTACAGGA AACCTACCTT AAGAAGCATT ACCTCTCCTT TGGTTGCTCA CAGACTAAAA 1680

CCAATCAGAC AGAAAACCAA AAAGGCTGTG GTGAGCATCC TTGATTCAGA GGAGGTGTGT 1740

GTGGAGCTTC TAAAAGAGTG TACATCTGAA GGACATGTGA AAGAAGTTCT TCAGATATCT 1800

AGTGATGGGA CTACGATCAC CGTTTATTAC CCAAATGATG GAAGAGGCTT TCCTCTTGCT 1860

GATAGACCAC CCTTGCCTAC TGACAACATC AGCAGGTACA GCTTTGACAG TTTACCAGAA 1920

AAATACTGGC GGAAATATCA GTATGCTTCC AGATTCATTC AGCTTGTAAG GTCCAAAACT 1980

CCCAAAATCA CTTACTTTAC AAGATATGCT AAATGTATTT TGATGGAAAA TTCTCCTGGT 2040

GCTGATTTTG AAGTTTGGTT TTATGATGGA GCCAAAATAC ATAAAACAGA AGATGTAATT 2100

CACATAATTG AAAAAACTGG GTTATCTTAC ACTTTAAAAA ATGAGAATGA CTTTACCAGC 2160

TTGAAAGAGG AAGTAAAAAT CTATATGGAC CATGCCAATG AGGGTCACCG TACTTGCTTG 2220

GCACTGGAAT CTGTAATCTC TGAAGAGGAA AAAAGAAGCA GGGGCTCTTC ATTCTTCCCA 2280

ATAATTGTAG GAAGAAAACC TGGTACTACT AGTTCACCTA AAGCCTTATC ACCACCTCCT 2340

GTGGACCCAG GCTACTCAAA GGGAGAGCAA GCATCATCAA GCAGATTGAG CGCGAATAGT 2400

GCGGCTTTCC CAACACAGAC ACCAGTACTC AGTCCTTCTG CTGTGACAGT GGAAGGACCT 2460

GGCCAAACAG CAGCTACTAC AGGAACAAGC ATCTCTTCAA GTCTTCCTAA ATCAGCACAG 2520

CTTTTGAAAT CTGTTTTTGT GAAAAATGTT GGTTGGGCTA CACAGCTAAC TAGTGGAGCT 2580

GTGTGGGTTC AGTTTAATGA TGGGTCACAG TTGGTTGTCC AGGCAGGAGT ATCTTCCATC 2640

AGTTATACAT CACCAGACGG TCAGACAACT AGGTATGGAG AAAATGAAAA ATTGCCTGAA 2700

TACATCAAAC AGAAATTGCA ATGTCTTTCT TCCATCCTTC TGATGTTTTC TAATCCAACT 2760

CCTAGTTTTC AGTAA 2775

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         +++g+llGkG+fa +y++++++t+ ++a+k+++k++++k+ + ++v++e++ih++lkh++

Q: 12    FKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPS  71

         6899********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrr.kaltepevryylrqivsglkylhekr  119

         +++ly++fed+++vylvle+c++++++++lk+r k+++e+e+r++++qi++g++ylh++

Q: 72    VLELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRmKPFSESEARHFMHQIITGMLYLHSHG  131

         ********************************99**************************

S: 120   ilhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghsle  179

         ilhrdl+l+n++l++++++ki+dfGlat+l++++e+++tlcGtPnyi+Pe++++++h+le

Q: 132   ILHRDLTLSNILLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLE  191

         ************************************************************

S: 180   vdiWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePe  239

         +diWslGc++ytll+G+PPf+t+++k+t++k++ a+y++P+ ls+ea++li++ll+++P+

Q: 192   SDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPA  251

         ************************************************************

S: 240   erpsleevlkdefl  253

         +r+sl++vl+++f+

Q: 252   DRLSLSSVLDHPFM  265

         ************98

Domain Sequence
(FASTA)

FKVGNLLGKG SFAGVYRAES IHTGLEVAIK MIDKKAMYKA GMVQRVQNEV KIHCQLKHPS 60

VLELYNYFED NNYVYLVLEM CHNGEMNRYL KNRMKPFSES EARHFMHQII TGMLYLHSHG 120

ILHRDLTLSN ILLTRNMNIK IADFGLATQL KMPHEKHYTL CGTPNYISPE IATRSAHGLE 180

SDIWSLGCMF YTLLIGRPPF DTDTVKNTLN KVVLADYEMP AFLSREAQDL IHQLLRRNPA 240

DRLSLSSVLD HPFM 254

Keyword

ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007099	; P:centriole replication
GO:0046601	; P:positive regulation of centriole replication
GO:0060707	; P:trophoblast giant cell differentiation

KEGG

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008266	; Tyr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013