EKS-RAN-00275

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00275

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	428.2	1.4E-128	79	331	253

Status

Reviewed

Ensembl Protein

ENSRNOP00000016768

UniProt Accession

Q9R012;

Protein Name

Serine/threonine-protein kinase PLK2

Protein Synonyms/Alias

Polo-like kinase 2; PLK-2; Serine/threonine-protein kinase SNK; Serum-inducible kinase;

Gene Name

Plk2

Gene Synonyms/Alias

Plk2; Snk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000011951	ENSRNOP00000016768	ENSRNOT00000016768

Organism

Rattus norvegicus

Functional Description

Tumor suppressor serine/threonine-protein kinaseinvolved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.

Protein Length

682

Protein Sequence
(FASTA)

MELLRTITYQ PAAGTKMCEQ ALGKACGGDS KKKRPQQPSE DGQSQAQVTP AAPHHHHHHS 60

HSGPEISRII VDPTTGKRYC RGKVLGKGGF AKCYEMTDLT NNKVYAAKII PHSRVAKPHQ 120

REKIDKEIEL HRILHHKHVV QFYHYFEDKE NIYILLEYCS RRSMAHILKA RKVLTEPEVR 180

YYLRQIVSGL KYLHEQEILH RDLKLGNFFI NEAMELKVGD FGLAARLEPL EHRRRTICGT 240

PNYLSPEVLN KQGHGCESDI WALGCVMYTM LLGRPPFETT NLKETYRCIR EARYTMPSSL 300

LAPAKHLIAS MLSKNPEDRP SLDDIIRHDF FLQGFTPDRL SSSCCHTVPD FHLSSPAKNF 360

FKKAAAALFG GKKDKARYND THNKVSKEDE DIYKLRHDLK KTSITQQPSK HRTDEELQPP 420

PTTFAKSGTS AVENKQQIGD AIRMIVRGTL GSCSSSSECL EDSTMGSVAD TVARVLRGCL 480

ENMPEADCIP KEQLSTSFQW VTKWVDYSNK YGFGYQLSDH TVGVLFNNGA HMSLLPDKKT 540

VHYYAELGQC SVFPATDAPE QFISQVTVLK YFSHYMEENL MDGGDLPSVT DIRRPRLYLL 600

QWLKSDKALM MLFNDGTFQV NFYHDHTKII ICNQNEEYLL TYINEDRIST TFRLTTLLMS 660

GCSLELKHRM EYALNMLLQR CN 682

Nucleotide Sequence
(FASTA)

ATGGAGCTCC TGCGGACTAT CACCTACCAG CCGGCCGCCG GCACCAAGAT GTGCGAGCAG 60

GCGCTGGGCA AAGCTTGCGG CGGGGACTCA AAGAAGAAGC GACCGCAGCA GCCTTCTGAA 120

GACGGGCAGT CCCAAGCCCA GGTGACCCCG GCGGCCCCAC ACCACCATCA TCACCATTCT 180

CACTCCGGAC CCGAGATCTC GCGGATTATA GTCGACCCCA CGACGGGGAA GCGCTACTGC 240

CGGGGCAAAG TGCTGGGCAA GGGTGGATTT GCAAAGTGTT ACGAAATGAC AGATCTGACA 300

AACAACAAAG TCTACGCTGC AAAAATTATC CCTCACAGCA GAGTAGCTAA ACCTCATCAA 360

AGGGAAAAGA TCGACAAAGA AATTGAGCTT CACAGAATCC TGCACCATAA GCATGTAGTG 420

CAGTTTTACC ACTACTTTGA AGACAAAGAA AACATTTACA TCCTCTTGGA GTACTGCAGT 480

AGAAGGTCCA TGGCTCATAT CTTGAAAGCA AGAAAGGTGT TGACAGAGCC AGAAGTCCGA 540

TACTACCTCA GGCAGATTGT GTCGGGACTC AAGTATCTTC ACGAACAAGA AATCTTGCAT 600

AGGGATCTCA AGCTAGGGAA CTTTTTTATT AATGAGGCCA TGGAGCTGAA GGTTGGAGAC 660

TTCGGCTTGG CTGCCAGACT GGAACCTTTG GAACACAGAA GGAGAACAAT ATGTGGAACC 720

CCAAATTATC TCTCCCCTGA AGTCCTCAAC AAACAAGGAC ATGGCTGTGA ATCAGACATC 780

TGGGCCTTAG GCTGTGTAAT GTATACAATG CTGCTAGGAA GACCTCCATT CGAAACAACA 840

AATCTGAAAG AAACGTACAG GTGCATAAGA GAAGCAAGAT ATACAATGCC GTCTTCATTA 900

CTGGCCCCCG CCAAGCACTT GATAGCCAGC ATGCTGTCCA AAAACCCAGA GGACCGCCCC 960

AGTTTGGATG ACATCATTCG GCATGACTTC TTTCTGCAGG GCTTCACTCC AGACAGACTC 1020

TCTTCTAGCT GTTGCCACAC AGTTCCAGAC TTCCACTTGT CAAGCCCAGC TAAGAATTTC 1080

TTTAAGAAAG CTGCTGCCGC TCTTTTTGGT GGCAAAAAGG ACAAAGCAAG ATATAATGAC 1140

ACACACAATA AAGTGTCTAA GGAAGATGAA GACATCTACA AGCTTCGGCA TGATTTGAAG 1200

AAAACATCGA TAACCCAGCA ACCCAGCAAA CACAGAACAG ATGAGGAGCT CCAGCCTCCT 1260

CCAACTACCT TTGCCAAATC TGGGACATCC GCAGTGGAAA ACAAACAGCA GATTGGAGAT 1320

GCAATCCGGA TGATAGTTAG AGGGACGCTG GGCAGCTGCA GCAGCAGCAG TGAATGCCTT 1380

GAGGATAGCA CCATGGGAAG TGTTGCAGAC ACAGTGGCAA GAGTCCTTCG AGGGTGTCTA 1440

GAAAACATGC CTGAAGCTGA CTGTATTCCC AAAGAGCAGT TGAGCACGTC CTTTCAGTGG 1500

GTCACCAAAT GGGTCGACTA CTCCAACAAA TACGGCTTTG GATACCAGCT CTCAGACCAC 1560

ACTGTTGGAG TCCTTTTCAA CAACGGCGCT CACATGAGCC TCCTTCCAGA CAAAAAGACA 1620

GTTCACTATT ATGCGGAACT TGGCCAATGC TCTGTTTTCC CAGCAACAGA TGCCCCCGAA 1680

CAATTTATTA GTCAAGTGAC GGTGCTGAAA TACTTTTCCC ACTACATGGA GGAGAACCTC 1740

ATGGATGGTG GCGATCTCCC TAGTGTTACT GACATTCGAA GACCTCGGCT CTACCTCCTT 1800

CAGTGGTTAA AGTCTGATAA AGCCTTAATG ATGCTCTTCA ATGACGGCAC ATTTCAGGTG 1860

AATTTCTACC ACGATCATAC AAAGATAATT ATCTGTAACC AAAATGAAGA ATACCTTCTC 1920

ACATACATCA ACGAAGACAG GATATCTACA ACTTTCAGAC TGACGACCCT GTTGATGTCT 1980

GGCTGTTCGC TAGAATTGAA ACATCGAATG GAATACGCCC TGAACATGCT CTTGCAGAGA 2040

TGTAACTGA 2049

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y+rgk+lGkGgfakcye++dl++++v+a+k++p+s++ak++qrek++keie+hr l+hk+

Q: 79    YCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHKH  138

         99**********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         +v++y++fedken+y++le+csrrs+a++lk+rk+ltepevryylrqivsglkylhe++i

Q: 139   VVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEI  198

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         lhrdlklgn+f+ne++e+k+gdfGla+rle+ e+r++t+cGtPny++Pevl+k+gh++e+

Q: 199   LHRDLKLGNFFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCES  258

         ************************************************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         diW+lGc++yt+l+G+PPfet++lkety++i++a+y++Pssl ++ak+lia++l+k+Pe+

Q: 259   DIWALGCVMYTMLLGRPPFETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPED  318

         ************************************************************

S: 241   rpsleevlkdefl  253

         rpsl+++++++f+

Q: 319   RPSLDDIIRHDFF  331

         ************8

Domain Sequence
(FASTA)

YCRGKVLGKG GFAKCYEMTD LTNNKVYAAK IIPHSRVAKP HQREKIDKEI ELHRILHHKH 60

VVQFYHYFED KENIYILLEY CSRRSMAHIL KARKVLTEPE VRYYLRQIVS GLKYLHEQEI 120

LHRDLKLGNF FINEAMELKV GDFGLAARLE PLEHRRRTIC GTPNYLSPEV LNKQGHGCES 180

DIWALGCVMY TMLLGRPPFE TTNLKETYRC IREARYTMPS SLLAPAKHLI ASMLSKNPED 240

RPSLDDIIRH DFF 253

Keyword

ATP-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0030425	; C:dendrite
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004871	; F:signal transducer activity
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0060292	; P:long term synaptic depression
GO:0060291	; P:long-term synaptic potentiation
GO:0007613	; P:memory
GO:0007093	; P:mitotic cell cycle checkpoint
GO:0007052	; P:mitotic spindle organization
GO:0043066	; P:negative regulation of apoptotic process
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0032486	; P:Rap protein signal transduction
GO:0046599	; P:regulation of centriole replication

KEGG

rno:83722

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013