EKS-RAN-00471

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00471

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/VPS15	505.4	5.0E-152	26	310	285

Status

Reviewed

Ensembl Protein

ENSRNOP00000018654

UniProt Accession

P0C0R5;

Protein Name

Phosphoinositide 3-kinase regulatory subunit 4

Protein Synonyms/Alias

PI3-kinase regulatory subunit 4;

Gene Name

Pik3r4

Gene Synonyms/Alias

Pik3r4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000013669	ENSRNOP00000018654	ENSRNOT00000018654

Organism

Rattus norvegicus

Functional Description

Regulatory subunit of the PI3K complex. May regulatemembrane trafficking late in the endocytic pathway (By similarity).

Protein Length

1358

Protein Sequence
(FASTA)

MGNQLAGIAP SQILSVESYF SDIHDFEYDK SLGSTRFFKV ARAKHREGLV VVKVFAIQDP 60

TLPLTSYKQE LEELKIRLHS AQNCLPFQKA AEKASEKAAM LFRQYVRDNL YDRISTRPFL 120

NNIEKRWIAF QILTAVDQAH KSGVRHGDIK TENVMVTSWN WVLLTDFASF KPTYLPEDNP 180

ADFNYFFDTS RRRTCYIAPE RFVDGGMFAT ELEYMRDPST PLVDLNSNQR TRGELKRAMD 240

IFSAGCVIAE LFTEGVPLFD LSQLLAYRNG HFFPEQVLNK IEDRSIRELV TQMIQREPGQ 300

RLEADDYLKQ QRGNAFPEVF YTFLQPYMAQ FAKETFLSAD ERILVIRKDL GNIIHNLCGH 360

DLPEKAEGES KASGLVVLVS VITSCLQTLK SCDSKLAALE LILHLAPRLS VEILLDRITP 420

YLLHFSNNSV PRVRAEALRT LTKVLALVQE VPRNDVNIYP EYILPGIAHL AQDDATIVRL 480

AYAENIALLA ETALRFLELV QLKTLNMENE PDSEEVDEAT RPNGDYDTEL QALHEMVQQK 540

VVTLLSDPEN IVKQTLMENG ITRLCVFFGR QKANDVLLSH MITFLNDKND WHLRGAFFDS 600

IVGVAAYVGW QSSSILKPLL QQGLSDAEEF VIVKALNALT CMCQLGLLQK PHVYEFASDI 660

APFLCHPNLW IRYGAVGFIT VVAHQISTAD VYCKLMPYLD PYITQPVIQI ERKLVLLSVL 720

KEPVSRSIFD YALRSKDIAS LFRHLHMRQK KRNGSLLDCP PPEDPAIAQL LKKLLSQGMT 780

EEEEDKLLAL KDFMMKSNRA KANAVDQSHL HDSSQKGVID LAALGITGRQ VDLVKTKQEP 840

DEKRARKHVK QDSNVNEEWK SMFGSLEPPN IPQALPKTSD HEVVPTGKSP RSESSAGVCV 900

PLSTSPQVSE AAHIPSKKPV IPVVSSTVLP STYQIRITTC KTELQQLIQQ KREQCNAERI 960

AKQMMENAEW ESKPPPPGWR PKGLLVAHLH EHKSAVNRIR VSDEHLLFAT CSNDGTVKIW 1020

NSQKMEGKTT TTRSILTYSR IGGRVKTLTF CQGSHYLAIA SDNGAVQLLG IEASKLPKSP 1080

KIHPLQSRIL DQKEDGCVVD MHHFNSGAQS VLAYATVNGS LVGWDLRSSS NAWTLKHDLK 1140

SGLITSFAVD IHQCWLCIGT SSGAMACWDM RFQLPISSHC HPSRARIRRL SMHPLYQSWV 1200

IAAVQGNNEV SMWDMETGDR RLTLWASSAP PLSELQPSPH SVHGIYCSPA DGNPILLTAG 1260

SDMKIRFWDL VSPERSYVVA GSTGSPSVSY YKKIIEGTEV VQEIQNKQKV GPSDDTPRRG 1320

PESLPVGHHD IITDIATFQT TQGFIVTASR DGIVKVWK 1358

Nucleotide Sequence
(FASTA)

ATGGGAAACC AACTAGCTGG TATTGCTCCT TCCCAGATCC TGTCCGTAGA GAGCTACTTC 60

TCAGACATCC ATGACTTTGA ATATGATAAG AGTCTGGGAA GTACTCGGTT TTTTAAAGTT 120

GCTCGAGCGA AGCACCGGGA GGGCCTGGTG GTTGTGAAGG TCTTTGCAAT CCAGGATCCC 180

ACGTTGCCTT TAACTAGTTA TAAACAGGAG CTGGAGGAAC TGAAAATCAG GCTCCATTCG 240

GCCCAGAACT GCTTACCTTT CCAGAAAGCA GCAGAAAAAG CATCGGAGAA AGCGGCCATG 300

CTGTTCAGGC AGTACGTGAG GGACAACCTC TACGACCGTA TCAGTACCCG TCCCTTCTTA 360

AATAACATCG AGAAGCGCTG GATCGCCTTC CAGATCCTGA CAGCTGTGGA CCAAGCACAC 420

AAGTCTGGAG TCCGCCACGG TGACATCAAG ACAGAGAACG TGATGGTCAC CAGTTGGAAC 480

TGGGTTCTCC TCACTGATTT TGCCAGTTTT AAGCCCACTT ATCTTCCAGA GGACAACCCA 540

GCAGATTTCA ACTATTTCTT TGACACCTCG AGGAGGAGGA CTTGCTACAT CGCCCCTGAG 600

CGTTTCGTGG ATGGCGGTAT GTTCGCCACC GAGTTAGAAT ACATGAGAGA TCCCTCAACT 660

CCTCTTGTCG ACCTAAATAG CAATCAGAGA ACACGGGGAG AGTTGAAGAG GGCCATGGAC 720

ATCTTTTCAG CAGGTTGCGT GATAGCCGAG CTCTTCACGG AAGGCGTGCC GTTGTTCGAT 780

CTCTCACAAC TGTTGGCATA CAGAAATGGA CATTTTTTCC CTGAGCAAGT GCTAAATAAA 840

ATCGAAGATC GCAGTATCAG AGAATTGGTA ACTCAGATGA TCCAGCGGGA GCCGGGACAG 900

CGTTTGGAAG CTGACGACTA CCTGAAGCAG CAGCGTGGCA ATGCTTTCCC CGAGGTATTC 960

TATACTTTCC TTCAGCCGTA CATGGCTCAG TTCGCCAAGG AGACCTTTCT CTCTGCAGAT 1020

GAGCGGATTT TGGTTATACG GAAAGACTTG GGCAATATCA TTCACAACCT CTGTGGACAT 1080

GACTTGCCAG AGAAAGCAGA AGGGGAGTCC AAGGCTAGCG GGCTGGTTGT CCTGGTGTCA 1140

GTGATAACGT CCTGCCTGCA GACACTGAAG TCCTGCGACT CCAAACTGGC TGCCTTGGAG 1200

CTCATTCTGC ATTTGGCCCC GAGGCTGAGC GTAGAGATCC TTCTGGATCG CATCACCCCC 1260

TACTTGTTGC ATTTCAGCAA CAACTCTGTT CCTCGAGTGC GGGCTGAAGC CCTGAGGACA 1320

CTCACCAAAG TCCTTGCCCT TGTCCAAGAA GTTCCTCGCA ATGATGTCAA TATCTACCCA 1380

GAGTACATTC TCCCCGGCAT AGCTCACCTG GCCCAGGACG ACGCCACCAT CGTCAGACTG 1440

GCCTACGCTG AAAACATAGC TCTGTTGGCC GAGACGGCTC TGCGATTCCT GGAACTAGTG 1500

CAGCTAAAAA CTCTCAACAT GGAGAACGAG CCGGACAGTG AAGAGGTAGA TGAAGCCACG 1560

CGTCCTAACG GAGACTATGA CACAGAGCTC CAGGCCCTAC ACGAAATGGT CCAGCAGAAG 1620

GTTGTCACTT TGTTAAGTGA CCCTGAGAAT ATTGTGAAAC AGACATTGAT GGAGAATGGG 1680

ATCACACGCT TGTGCGTCTT CTTTGGACGG CAGAAAGCCA ACGACGTTCT CTTGTCCCAC 1740

ATGATCACGT TCCTGAATGA TAAGAATGAC TGGCACCTGC GTGGAGCTTT CTTCGATAGC 1800

ATAGTCGGTG TGGCTGCCTA TGTTGGCTGG CAGAGCTCCT CCATCCTCAA GCCCCTGCTC 1860

CAGCAAGGCC TCAGCGATGC CGAGGAGTTC GTCATTGTGA AAGCTCTCAA TGCCCTGACC 1920

TGTATGTGCC AGTTAGGGCT GCTGCAGAAA CCTCATGTCT ATGAATTCGC CAGTGATATT 1980

GCTCCCTTCC TGTGTCACCC TAATCTGTGG ATACGCTATG GTGCCGTGGG ATTCATCACC 2040

GTGGTAGCTC ATCAGATCAG CACTGCTGAC GTCTACTGCA AGCTCATGCC GTATCTTGAC 2100

CCGTATATTA CACAACCAGT AATACAGATT GAAAGGAAGC TTGTCTTGCT TAGCGTTCTA 2160

AAAGAGCCGG TGAGTCGCTC TATATTTGAT TATGCTCTGA GGTCCAAAGA CATCGCTAGC 2220

TTGTTCAGAC ATCTTCACAT GCGTCAGAAG AAGCGGAATG GCTCTCTTCT TGACTGCCCT 2280

CCGCCCGAGG ACCCTGCCAT AGCACAGCTT CTGAAGAAAC TGCTCTCCCA GGGGATGACC 2340

GAAGAAGAGG AAGATAAACT TCTGGCTCTG AAGGACTTCA TGATGAAATC AAATCGAGCA 2400

AAAGCTAATG CAGTAGACCA GAGCCACCTG CATGACAGCA GCCAGAAAGG TGTAATTGAC 2460

TTGGCAGCCT TGGGCATCAC TGGGAGACAA GTTGACCTTG TTAAAACCAA GCAAGAGCCC 2520

GATGAGAAAC GGGCTAGGAA GCATGTGAAA CAAGACTCCA ATGTAAATGA AGAATGGAAA 2580

AGCATGTTTG GGTCACTGGA ACCACCGAAT ATCCCACAGG CCCTGCCTAA AACCAGTGAC 2640

CATGAGGTGG TTCCAACGGG GAAGTCACCT CGCTCTGAGT CCTCTGCTGG TGTCTGTGTC 2700

CCTTTGTCAA CCTCTCCACA GGTTTCCGAA GCAGCACACA TCCCCAGTAA GAAGCCGGTG 2760

ATTCCGGTTG TAAGTAGCAC GGTCCTGCCA TCCACTTACC AGATCCGGAT CACCACTTGC 2820

AAGACTGAAC TTCAGCAGCT CATACAACAG AAGCGTGAGC AGTGTAACGC AGAGCGCATA 2880

GCCAAGCAGA TGATGGAGAA CGCCGAGTGG GAGAGCAAGC CACCTCCACC TGGGTGGCGT 2940

CCTAAAGGGC TCCTAGTTGC ACATCTTCAT GAGCACAAAT CTGCTGTAAA TCGAATCAGA 3000

GTCTCCGATG AACACTTACT CTTTGCAACG TGCTCAAATG ATGGTACAGT GAAAATCTGG 3060

AACAGTCAGA AGATGGAGGG GAAGACCACT ACGACGAGGT CTATTCTGAC ATACAGCCGA 3120

ATTGGAGGGC GAGTCAAGAC ACTAACATTT TGCCAAGGCT CCCACTACTT GGCCATAGCA 3180

TCTGATAATG GTGCTGTCCA GCTTCTTGGA ATTGAGGCTT CTAAGTTACC CAAGTCTCCT 3240

AAAATCCACC CTCTACAAAG CAGGATTCTG GATCAGAAGG AAGACGGATG TGTGGTGGAC 3300

ATGCATCACT TCAACTCGGG GGCACAGTCT GTTCTTGCCT ATGCCACAGT GAATGGCTCT 3360

CTGGTTGGAT GGGATCTCAG GTCATCAAGC AACGCATGGA CATTAAAGCA TGACCTGAAG 3420

TCAGGCCTCA TCACCTCCTT TGCTGTGGAC ATCCACCAGT GCTGGCTGTG CATAGGCACG 3480

AGCAGCGGGG CCATGGCGTG TTGGGACATG AGGTTCCAGT TGCCAATTTC CAGTCACTGT 3540

CATCCCTCCA GAGCTCGTAT CCGGCGCCTC TCCATGCACC CCTTGTACCA GTCCTGGGTA 3600

ATTGCAGCTG TTCAGGGCAA CAATGAAGTC TCCATGTGGG ACATGGAGAC CGGAGACAGG 3660

AGACTGACTC TCTGGGCTAG CAGTGCGCCT CCCCTGTCTG AGTTACAGCC CTCACCGCAC 3720

AGCGTCCATG GCATCTACTG CAGCCCTGCG GATGGCAACC CTATCCTGCT GACCGCTGGC 3780

TCAGACATGA AAATAAGGTT CTGGGACTTG GTTTCCCCAG AGAGGTCCTA TGTTGTAGCG 3840

GGAAGCACGG GTTCCCCGTC TGTATCCTAC TACAAGAAGA TAATAGAAGG CACGGAGGTT 3900

GTCCAGGAAA TTCAGAATAA GCAGAAGGTT GGACCAAGTG ATGACACCCC TCGAAGGGGC 3960

CCGGAGTCTC TGCCTGTGGG ACATCATGAC ATCATCACAG ATATTGCCAC CTTCCAGACC 4020

ACTCAGGGCT TCATTGTGAC TGCTTCTAGA GATGGGATTG TGAAGGTGTG GAAATAA 4077

Domain Profile

S: 1     veyvknlgstrflkvakakteeGeivvkvfvkqdptlslesfkqrleelklklakaqncl  60

         +ey+k+lgstrf+kva+ak++eG++vvkvf++qdptl+l+s+kq+leelk++l++aqncl

Q: 26    FEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLHSAQNCL  85

         68**********************************************************

S: 61    pfqkvlek..ekaavllrqyvkdnlydrlstrPflneiekkwiafqllkaldqihksgvv  118

         pfqk++ek  ekaa+l+rqyv+dnlydr+strPfln+iek+wiafq+l+a+dq+hksgv+

Q: 86    PFQKAAEKasEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVR  145

         *******999**************************************************

S: 119   hGdiktenvlvtswnWvlltdfasfkPtylpednPadftyffdtsrrrtcyiaPerfvsa  178

         hGdiktenv+vtswnWvlltdfasfkPtylpednPadf+yffdtsrrrtcyiaPerfv++

Q: 146   HGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDG  205

         ************************************************************

S: 179   lasdekl...rdgstelvdldsiqrlrgelkkamdifsaGcvilelftegrplfelsqll  235

         ++++++l   rd+st+lvdl+s+qr+rgelk+amdifsaGcvi+elfteg+plf+lsqll

Q: 206   GMFATELeymRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLL  265

         ******9999**************************************************

S: 236   ayrsgeflvekvlekiededlrnlvlqliqlepekrksaedllkk  280

         ayr+g+f++e+vl+kied+++r+lv+q+iq+ep +r++a+d+lk+

Q: 266   AYRNGHFFPEQVLNKIEDRSIRELVTQMIQREPGQRLEADDYLKQ  310

         *******************************************97

Domain Sequence
(FASTA)

FEYDKSLGST RFFKVARAKH REGLVVVKVF AIQDPTLPLT SYKQELEELK IRLHSAQNCL 60

PFQKAAEKAS EKAAMLFRQY VRDNLYDRIS TRPFLNNIEK RWIAFQILTA VDQAHKSGVR 120

HGDIKTENVM VTSWNWVLLT DFASFKPTYL PEDNPADFNY FFDTSRRRTC YIAPERFVDG 180

GMFATELEYM RDPSTPLVDL NSNQRTRGEL KRAMDIFSAG CVIAELFTEG VPLFDLSQLL 240

AYRNGHFFPE QVLNKIEDRS IRELVTQMIQ REPGQRLEAD DYLKQ 285

Keyword

ATP-binding; Complete proteome; Endosome; Kinase; Lipoprotein; Myristate; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; WD repeat.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005770	; C:late endosome
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity

KEGG

InterPros

IPR011989	; ARM-like.
IPR016024	; ARM-type_fold.
IPR021133	; HEAT_type_2.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR015943	; WD40/YVTN_repeat-like_dom.
IPR001680	; WD40_repeat.
IPR019775	; WD40_repeat_CS.
IPR017986	; WD40_repeat_dom.

Pfam

PF00069	; Pkinase; 1.
PF00400	; WD40; 3.

SMARTs

SM00320

; WD40; 6.

Prosites

PS50077	; HEAT_REPEAT; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00678	; WD_REPEATS_1; 2.
PS50082	; WD_REPEATS_2; 3.
PS50294	; WD_REPEATS_REGION; 2.

Prints

Created Date

20-Feb-2013