EKS-RAN-00167

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00167

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	454.1	2.0E-136	351	614	264

Status

Reviewed

Ensembl Protein

ENSRNOP00000019825

UniProt Accession

P63319; P05697; Q5FWS3;

Protein Name

Protein kinase C gamma type

Protein Synonyms/Alias

PKC-gamma;

Gene Name

Prkcg

Gene Synonyms/Alias

Prkcg; Pkcc, Pkcg, Prkcc;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000014688	ENSRNOP00000019825	ENSRNOT00000019825

Organism

Rattus norvegicus

Functional Description

Calcium-activated, phospholipid- and diacylglycerol(DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53/TP53 and promotes p53/TP53- dependent apoptosis in response to DNA damage (By similarity).

Protein Length

697

Protein Sequence
(FASTA)

MAGLGPGGGD SEGGPRPLFC RKGALRQKVV HEVKSHKFTA RFFKQPTFCS HCTDFIWGIG 60

KQGLQCQVCS FVVHRRCHEF VTFECPGAGK GPQTDDPRNK HKFRLHSYSS PTFCDHCGSL 120

LYGLVHQGMK CSCCEMNVHR RCVRSVPSLC GVDHTERRGR LQLEIRAPTS DEIHITVGEA 180

RNLIPMDPNG LSDPYVKLKL IPDPRNLTKQ KTKTVKATLN PVWNETFVFN LKPGDVERRL 240

SVEVWDWDRT SRNDFMGAMS FGVSELLKAP VDGWYKLLNQ EEGEYYNVPV ADADNCSLLQ 300

KFEACNYPLE LYERVRMGPS SSPIPSPSPS PTDSKRCFFG ASPGRLHISD FSFLMVLGKG 360

SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP GGRPHFLTQL 420

HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD 480

LKLDNVMLDA EGHIKITDFG MCKENVFPGS TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS 540

FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS 600

GPDGEPTIRA HGFFRWIDWE RLERLEIAPP FRPRPCGRSG ENFDKFFTRA APALTPPDRL 660

VLASIDQADF QGFTYVNPDF VHPDARSPTS PVPVPVM 697

Nucleotide Sequence
(FASTA)

ATGGCGGGTC TGGGTCCTGG CGGGGGCGAC TCAGAAGGGG GACCCCGACC CCTGTTTTGC 60

AGAAAGGGGG CGCTGAGGCA GAAGGTGGTC CACGAGGTGA AGAGCCACAA GTTCACCGCT 120

CGTTTCTTCA AGCAGCCAAC CTTCTGCAGT CACTGTACCG ACTTCATCTG GGGCATTGGA 180

AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ACCGCCGATG CCACGAATTT 240

GTGACCTTCG AGTGTCCAGG CGCTGGAAAG GGCCCCCAGA CGGACGACCC TCGCAACAAG 300

CACAAGTTCC GTCTGCACAG CTACAGCAGT CCCACCTTCT GCGACCACTG TGGTTCCCTC 360

CTCTACGGGC TGGTGCACCA GGGCATGAAA TGTTCCTGTT GCGAAATGAA TGTGCACCGA 420

CGCTGTGTGC GCAGCGTGCC CTCCCTTTGC GGCGTGGACC ATACAGAGCG CCGTGGACGT 480

CTGCAACTGG AAATCCGGGC TCCCACATCA GATGAGATCC ATATTACTGT GGGTGAGGCC 540

CGGAACCTCA TTCCTATGGA CCCCAATGGC CTGTCTGATC CCTATGTGAA ACTGAAGCTC 600

ATCCCGGACC CTCGGAACCT GACAAAACAG AAGACAAAGA CCGTGAAAGC CACACTGAAT 660

CCCGTGTGGA ACGAGACCTT CGTGTTCAAC CTGAAGCCGG GGGATGTGGA GCGCCGGCTC 720

AGTGTGGAGG TGTGGGATTG GGATAGGACA TCCCGAAATG ACTTCATGGG TGCCATGTCC 780

TTTGGTGTCT CAGAGCTACT CAAGGCTCCT GTGGATGGAT GGTACAAGTT ACTGAACCAG 840

GAGGAGGGCG AGTATTACAA TGTACCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 900

AAGTTTGAGG CCTGTAATTA CCCCTTGGAA TTGTATGAGA GAGTGCGGAT GGGCCCCTCT 960

TCCTCTCCCA TTCCTTCTCC ATCCCCCAGT CCCACGGACT CCAAGAGATG CTTCTTCGGT 1020

GCCAGCCCAG GACGCCTGCA TATCTCTGAC TTCAGCTTCC TCATGGTTCT AGGGAAAGGC 1080

AGTTTTGGGA AGGTGATGCT GGCAGAGCGC AGAGGATCCG ATGAACTCTA TGCCATCAAG 1140

ATACTGAAAA AAGACGTCAT TGTCCAGGAT GATGATGTAG ACTGCACCCT TGTGGAGAAG 1200

CGTGTGCTGG CATTGGGAGG CCGAGGTCCT GGAGGCCGGC CACACTTTCT CACACAACTT 1260

CATTCCACCT TTCAGACTCC GGACCGCCTG TATTTTGTGA TGGAGTACGT CACTGGGGGC 1320

GATTTAATGT ACCACATTCA GCAACTGGGC AAGTTTAAGG AGCCCCACGC AGCATTCTAT 1380

GCCGCGGAAA TCGCCATAGG CCTCTTCTTC CTTCACAACC AGGGCATCAT CTACAGGGAC 1440

CTCAAGTTGG ATAATGTGAT GCTGGATGCT GAAGGACACA TCAAGATCAC AGACTTCGGC 1500

ATGTGTAAAG AGAATGTCTT CCCTGGGTCC ACAACCCGCA CCTTCTGTGG GACCCCAGAC 1560

TACATAGCAC CTGAGATCAT TGCCTATCAG CCCTATGGGA AGTCTGTCGA CTGGTGGTCC 1620

TTTGGAGTCC TGCTGTATGA GATGTTGGCA GGACAGCCAC CCTTTGATGG GGAAGATGAG 1680

GAGGAGCTGT TTCAAGCCAT CATGGAACAA ACTGTCACCT ATCCCAAGTC ACTTTCCCGG 1740

GAAGCTGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGAAAGCG CCTGGGCTCA 1800

GGGCCAGATG GGGAACCCAC CATCCGGGCT CATGGCTTTT TCCGTTGGAT CGATTGGGAG 1860

AGGTTGGAGA GACTGGAAAT TGCGCCTCCT TTTAGACCAC GTCCGTGTGG CCGCAGCGGC 1920

GAAAACTTTG ACAAGTTCTT CACGCGGGCA GCGCCAGCCT TGACCCCGCC AGACCGCTTG 1980

GTCCTAGCCA GCATCGACCA AGCTGATTTC CAGGGCTTTA CTTATGTGAA CCCGGACTTC 2040

GTGCACCCAG ATGCCCGCAG CCCCACAAGC CCTGTGCCTG TGCCCGTCAT GTAA 2094

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlal.....  55

         f++l+vlGkGsfgkv+lae++++delyaik+lkkdv++qdddv++tlvekrvlal

Q: 351   FSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALggrgp  410

         89*****************************************************66666

S: 56    askkpflvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkf  115

           +++fl+ql+s+fqt drl+fv+eyv+GGdlm+hiq+ +k+ke++a+fyaaei+++l+f

Q: 411   GGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFF  470

         67889*******************************************************

S: 116   lhekgiiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkee  175

         lh++giiyrdlkldnv+ldaeGhik++dfG+cke+++ g+tt+tfcGtPdyiaPei++++

Q: 471   LHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGSTTRTFCGTPDYIAPEIIAYQ  530

         ************************************************************

S: 176   eygksvdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkgll  235

         +ygksvdww++Gvllyemlagq+Pf+gede+elf++i+e++v+yPksls+eav+i+kg+l

Q: 531   PYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFL  590

         ************************************************************

S: 236   tkdpekrlGvkeegeedikehaff  259

         tk+p krlG+ ++ge +i++h ff

Q: 591   TKHPGKRLGSGPDGEPTIRAHGFF  614

         ***********************8

Domain Sequence
(FASTA)

FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP 60

GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF 120

LHNQGIIYRD LKLDNVMLDA EGHIKITDFG MCKENVFPGS TTRTFCGTPD YIAPEIIAYQ 180

PYGKSVDWWS FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL 240

TKHPGKRLGS GPDGEPTIRA HGFF 264

Keyword

3D-structure; Acetylation; ATP-binding; Calcium; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Kinase; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Synapse; Synaptosome; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pongo abelii"; ?>Sus scrofa"; ?>Tursiops truncatus"; ?>

Gene Ontology

GO:0030054	; C:cell junction
GO:0005829	; C:cytosol
GO:0030425	; C:dendrite
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005886	; C:plasma membrane
GO:0097060	; C:synaptic membrane
GO:0005524	; F:ATP binding
GO:0004698	; F:calcium-dependent protein kinase C activity
GO:0008270	; F:zinc ion binding
GO:0007635	; P:chemosensory behavior
GO:0060384	; P:innervation
GO:0035556	; P:intracellular signal transduction
GO:0007611	; P:learning or memory
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:0042177	; P:negative regulation of protein catabolic process
GO:0031397	; P:negative regulation of protein ubiquitination
GO:0032425	; P:positive regulation of mismatch repair
GO:0046777	; P:protein autophosphorylation
GO:0043278	; P:response to morphine
GO:0048265	; P:response to pain
GO:0007268	; P:synaptic transmission

KEGG

rno:24681

;

InterPros

IPR000961	; AGC-kinase_C.
IPR000008	; C2_Ca-dep.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR020477	; C2_dom.
IPR018029	; C2_membr_targeting.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014375	; Protein_kinase_C_a/b/g.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 2.
PF00168	; C2; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 2.
SM00239	; C2; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 2.
PS50081	; ZF_DAG_PE_2; 2.

Prints

PR00360	; C2DOMAIN.
PR00008	; DAGPEDOMAIN.

Created Date

20-Feb-2013