| Tag | Content |
|---|
| EKPD ID | EKS-RAN-00230 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CAMK/MAPKAPK | 426.3 | 5.2E-128 | 46 | 306 | 261 |
|
| Status | Reviewed |
| Ensembl Protein | ENSRNOP00000020065 |
| UniProt Accession | Q66H84; |
| Protein Name | MAP kinase-activated protein kinase 3 |
| Protein Synonyms/Alias | MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; |
| Gene Name | Mapkapk3 |
| Gene Synonyms/Alias | Mapkapk3; |
| Ensembl Information | |
| Organism | Rattus norvegicus |
| Functional Description | Stress-activated serine/threonine-protein kinaseinvolved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38- alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression (By similarity). |
| Protein Length | 384 |
Protein Sequence
(FASTA) | | MDGETAGEKG SLVPQPGALG APALGGAPAP GVRREPKKYA VTDDYQLSKQ VLGLGVNGKV 60 | | LECYHRRSGQ KCALKLLYDS PKARQEVDHH WQASGGPHIV RILDVYENMH HGKRCLLIVM 120 | | ECMEGGELFS RIQERGDQAF TEREAAEIMR DIGTAIQFLH SQNIAHRDVK PENLLYTSKE 180 | | KDAVLKLTDF GFAKETTQNA LQTPCYTPYY VAPEVLGPEK YDKSCDMWSL GVIMYILLCG 240 | | FPPFYSNTGQ AISPGMKRRI RLGQYGFPKP EWADVSEDAK QLIRLLLKTD PTERLTIMQF 300 | | MNHPWINQSM EVPQTPLHTA RVLEEDKDHW DDVKEEMTSA LATMRVDYDQ VKIKDLKTSN 360 | | NRLLNKRRKK QGGSSSASPG CNNQ 384 |
|
Nucleotide Sequence
(FASTA) | | ATGGATGGCG AGACGGCGGG GGAGAAGGGG AGTCTTGTGC CCCAGCCAGG TGCGCTCGGT 60 | | GCGCCCGCCT TGGGCGGTGC TCCGGCCCCA GGTGTGCGAC GGGAACCCAA GAAGTACGCG 120 | | GTGACTGATG ACTACCAGTT GTCCAAGCAA GTGCTGGGTC TGGGTGTGAA CGGCAAGGTA 180 | | CTAGAGTGCT ACCATCGGCG CTCTGGGCAG AAGTGTGCCT TGAAGCTCCT GTATGACAGC 240 | | CCCAAGGCCC GGCAGGAGGT GGACCACCAC TGGCAGGCCT CAGGCGGCCC CCACATTGTG 300 | | CGCATCCTGG ACGTGTACGA GAACATGCAT CACGGCAAGC GCTGCCTCCT CATCGTCATG 360 | | GAATGCATGG AGGGTGGTGA GCTGTTCAGC AGGATTCAGG AGCGCGGTGA TCAGGCTTTC 420 | | ACTGAGAGAG AGGCCGCAGA GATAATGCGG GACATTGGCA CTGCCATCCA GTTCTTGCAC 480 | | AGCCAGAACA TTGCCCACCG AGATGTCAAG CCTGAAAACC TACTCTATAC ATCCAAGGAG 540 | | AAGGATGCTG TACTTAAACT CACTGATTTT GGCTTTGCCA AGGAAACCAC CCAAAATGCC 600 | | CTCCAGACAC CCTGTTACAC TCCCTATTAT GTGGCTCCTG AGGTCCTGGG TCCAGAGAAG 660 | | TATGACAAGT CGTGCGATAT GTGGTCCCTG GGGGTCATCA TGTACATCCT TTTGTGTGGA 720 | | TTCCCACCCT TCTACTCCAA CACGGGCCAG GCCATCTCTC CAGGAATGAA GAGAAGGATT 780 | | CGCTTGGGCC AGTATGGCTT CCCTAAACCT GAATGGGCAG ACGTCTCTGA GGATGCCAAG 840 | | CAGCTAATCC GTCTGCTCCT GAAGACAGAT CCCACCGAGA GGCTGACCAT CATGCAGTTT 900 | | ATGAACCATC CTTGGATCAA TCAATCGATG GAGGTCCCAC AGACCCCACT CCATACAGCC 960 | | CGAGTGCTGG AGGAAGACAA AGACCACTGG GATGACGTCA AGGAAGAGAT GACCAGTGCC 1020 | | CTGGCCACGA TGAGGGTAGA CTACGACCAG GTGAAAATCA AGGACCTGAA GACCTCTAAC 1080 | | AACCGACTCC TCAACAAGCG GAGGAAAAAG CAGGGGGGCA GCTCCTCAGC CTCACCGGGA 1140 | | TGCAACAACC AGTAA 1155 |
|
| Domain Profile | S: 1 klteevlGeGisgkvlecvkkktgekyalkilkdrkkvrrevelhvqasghknivelidv 60 | +l+++vlG G++gkvlec+++++g+k+alk+l+d++k+r+ev++h+qasg ++iv+++dv | Q: 46 QLSKQVLGLGVNGKVLECYHRRSGQKCALKLLYDSPKARQEVDHHWQASGGPHIVRILDV 105 | 5789******************************************************** |
| S: 61 fensfedekrlllvlekleGGellsriqer..kaftekeasevvkdiasalkflhsknia 118 | +en+++++++ll+v+e++eGGel+sriqer +afte+ea+e+++di++a++flhs+nia | Q: 106 YENMHHGKRCLLIVMECMEGGELFSRIQERgdQAFTEREAAEIMRDIGTAIQFLHSQNIA 165 | *******************************9**************************** |
| S: 119 hrDlkpenlLyeskeknapvklcDfglaketeeeeelktpcltaeyvaPevleaekeeal 178 | hrD+kpenlLy+skek+a++kl+Dfg+ake +++++l+tpc+t++yvaPevl++ek | Q: 166 HRDVKPENLLYTSKEKDAVLKLTDFGFAKE-TTQNALQTPCYTPYYVAPEVLGPEK---- 220 | *****************************7.7899*********************.... |
| S: 179 lydksCDlWslGvilyillcGypPfyskkgraiskklkeriqegkyefPeeeWsevseea 238 | ydksCD+WslGvi+yillcG+pPfys++g+ais+++k+ri+ g+y fP++eW++vse+a | Q: 221 -YDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPKPEWADVSEDA 279 | .*********************************************************** |
| S: 239 kdlirklLkrdpkerltieevlnhpwv 265 | k+lir lLk+dp+erlti++++nhpw+ | Q: 280 KQLIRLLLKTDPTERLTIMQFMNHPWI 306 | **************************8 |
|
Domain Sequence
(FASTA) | | QLSKQVLGLG VNGKVLECYH RRSGQKCALK LLYDSPKARQ EVDHHWQASG GPHIVRILDV 60 | | YENMHHGKRC LLIVMECMEG GELFSRIQER GDQAFTEREA AEIMRDIGTA IQFLHSQNIA 120 | | HRDVKPENLL YTSKEKDAVL KLTDFGFAKE TTQNALQTPC YTPYYVAPEV LGPEKYDKSC 180 | | DMWSLGVIMY ILLCGFPPFY SNTGQAISPG MKRRIRLGQY GFPKPEWADV SEDAKQLIRL 240 | | LLKTDPTERL TIMQFMNHPW I 261 |
|
| Keyword | Acetylation; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |