EKS-RAN-00170

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00170

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	439.0	7.9E-132	252	518	267

Status

Reviewed

Ensembl Protein

ENSRNOP00000021285

UniProt Accession

P09217;

Protein Name

Protein kinase C zeta type

Protein Synonyms/Alias

nPKC-zeta;

Gene Name

Prkcz

Gene Synonyms/Alias

Prkcz; Pkcz;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000015480	ENSRNOP00000021285	ENSRNOT00000021285

Organism

Rattus norvegicus

Functional Description

Calcium- and diacylglycerol-independentserine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukines production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In NF-kappa-B-mediated inflammatory response, can relieve the SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Is necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells.

Protein Length

592

Protein Sequence
(FASTA)

MPSRTDPKMD RSGGRVRLKA HYGGDILITS VDPTTTFQDL CEEVRDMCGL HQQHPLTLKW 60

VDSEGDPCTV SSQMELEEAF RLACQGRDEV LIIHVFPSIP EQPGMPCPGE DKSIYRRGAR 120

RWRKLYRANG HLFQAKRFNR RAYCGQCSER IWGLARQGYR CINCKLLVHK RCHVLVPLTC 180

RRHMDSVMPS QEPPVDDKND GVDLPSEETD GIAYISSSRK HDNIKDDSED LKPVIDGVDG 240

IKISQGLGLQ DFDLIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE 300

KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE EHARFYAAEI 360

CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE GLGPGDTTST FCGTPNYIAP 420

EILRGEEYGF SVDWWALGVL MFEMMAGRSP FDIITDNPDM NTEDYLFQVI LEKPIRIPRF 480

LSVKASHVLK GFLNKDPKER LGCRPQTGFS DIKSHAFFRS IDWDLLEKKQ TLPPFQPQIT 540

DDYGLDNFDT QFTSEPVQLT PDDEDVIKRI DQSEFEGFEY INPLLLSAEE SV 592

Nucleotide Sequence
(FASTA)

ATGCCCAGCA GGACCGACCC CAAGATGGAC CGGAGCGGCG GCCGCGTCCG TCTGAAGGCG 60

CACTACGGCG GGGACATCCT GATTACCAGC GTGGACCCCA CGACAACTTT CCAGGACCTC 120

TGTGAGGAAG TGCGAGACAT GTGTGGCCTG CACCAGCAGC ACCCACTCAC CCTCAAGTGG 180

GTGGACAGTG AAGGTGACCC TTGTACTGTG TCCTCACAGA TGGAGCTGGA GGAGGCCTTC 240

CGCCTGGCCT GTCAGGGCAG GGACGAAGTG CTCATCATCC ACGTTTTCCC AAGCATCCCA 300

GAACAACCGG GCATGCCTTG TCCTGGAGAA GACAAGTCCA TCTACCGCCG TGGAGCCAGA 360

AGATGGAGGA AGCTATACCG AGCCAACGGC CACCTCTTCC AAGCCAAGCG CTTTAACAGG 420

AGAGCGTACT GTGGCCAGTG CAGCGAAAGG ATATGGGGCC TCGCGAGGCA GGGGTACAGG 480

TGCATCAACT GCAAGCTGCT TGTCCATAAA CGCTGCCACG TCCTCGTCCC GCTGACCTGC 540

AGGAGGCATA TGGATTCTGT CATGCCTTCC CAAGAGCCTC CAGTAGATGA CAAGAACGAT 600

GGTGTAGACC TTCCTTCAGA AGAAACTGAT GGAATTGCTT ATATTTCTTC ATCTCGGAAA 660

CATGACAATA TCAAAGATGA TTCTGAGGAC CTTAAGCCTG TCATCGATGG GGTGGATGGG 720

ATCAAAATCT CTCAGGGGCT GGGGCTGCAA GACTTTGACC TCATCAGAGT CATCGGGCGT 780

GGAAGCTATG CCAAGGTCCT CCTGGTGCGG TTGAAGAAAA ACGACCAGAT TTACGCCATG 840

AAGGTGGTGA AGAAGGAGCT CGTCCACGAC GATGAGGATA TCGACTGGGT GCAGACAGAG 900

AAGCACGTGT TCGAGCAGGC ATCCAGCAAC CCCTTCCTGG TTGGCTTACA CTCCTGCTTC 960

CAGACAACGA GCCGGTTGTT CCTGGTCATC GAGTATGTCA ACGGGGGGGA CCTCATGTTC 1020

CACATGCAGA GGCAGAGGAA GCTTCCAGAG GAACACGCCA GGTTCTATGC TGCTGAGATC 1080

TGTATCGCTC TCAACTTCCT ACATGAGAGA GGGATCATCT ACCGGGACCT AAAACTGGAC 1140

AACGTCCTCC TCGATGCCGA TGGACACATT AAGCTGACGG ACTACGGCAT GTGCAAGGAA 1200

GGCCTAGGCC CCGGCGACAC AACAAGCACT TTTTGTGGAA CCCCGAACTA TATCGCCCCC 1260

GAAATCCTGC GAGGAGAAGA GTACGGGTTC AGCGTGGACT GGTGGGCGCT GGGTGTCCTT 1320

ATGTTTGAGA TGATGGCTGG GCGCTCCCCC TTTGACATCA TCACAGACAA CCCTGACATG 1380

AATACTGAAG ACTACCTTTT CCAAGTTATC CTGGAAAAGC CAATTCGGAT TCCCCGTTTC 1440

CTGTCTGTCA AGGCCTCACA CGTCTTGAAA GGATTTTTAA ATAAGGATCC CAAAGAGAGG 1500

CTTGGCTGCC GGCCGCAGAC TGGGTTTTCC GACATCAAGT CCCATGCCTT CTTCCGAAGC 1560

ATAGACTGGG ACCTGCTCGA AAAGAAGCAG ACCCTGCCTC CCTTCCAGCC CCAGATCACA 1620

GATGACTATG GCCTGGACAA CTTCGACACG CAGTTCACCA GCGAGCCCGT ACAGCTGACC 1680

CCAGATGATG AGGACGTCAT AAAGAGGATC GACCAGTCCG AGTTCGAAGG CTTCGAGTAC 1740

ATCAACCCGC TTCTGCTGTC TGCTGAGGAG TCCGTGTGA 1779

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp  60

         f+l++v+G+Gs++kvll++lkk+d++ya+kv+kk++v++d+d++++++ek+v+++as++p

Q: 252   FDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNP  311

         89**********************************************************

S: 61    flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg  120

         flv l+scfqt++rlf+v+eyvnGGdlmfh+q++rkl+ee+arfyaaei++al+flhe+g

Q: 312   FLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERG  371

         ************************************************************

S: 121   iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks  180

         iiyrdlkldnvllda+Ghikl+d+G+cke++  g+ttstfcGtP+yiaPeil++eeyg+s

Q: 372   IIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFS  431

         ************************************************************

S: 181   vdwwalGvllyemlagqsPfe.......gededelfesilekevlyPkslskeaveilkg  233

         vdwwalGvl++em+ag+sPf+       +++ed+lf++ilek +++P+ ls++a+++lkg

Q: 432   VDWWALGVLMFEMMAGRSPFDiitdnpdMNTEDYLFQVILEKPIRIPRFLSVKASHVLKG  491

         **************************999*******************************

S: 234   lltkdpekrlGvk.eegeedikehaff  259

         +l+kdp++rlG++ ++g +dik+haff

Q: 492   FLNKDPKERLGCRpQTGFSDIKSHAFF  518

         ************889***********9

Domain Sequence
(FASTA)

FDLIRVIGRG SYAKVLLVRL KKNDQIYAMK VVKKELVHDD EDIDWVQTEK HVFEQASSNP 60

FLVGLHSCFQ TTSRLFLVIE YVNGGDLMFH MQRQRKLPEE HARFYAAEIC IALNFLHERG 120

IIYRDLKLDN VLLDADGHIK LTDYGMCKEG LGPGDTTSTF CGTPNYIAPE ILRGEEYGFS 180

VDWWALGVLM FEMMAGRSPF DIITDNPDMN TEDYLFQVIL EKPIRIPRFL SVKASHVLKG 240

FLNKDPKERL GCRPQTGFSD IKSHAFF 267

Keyword

ATP-binding; Cell junction; Complete proteome; Cytoplasm; Endosome; Inflammatory response; Kinase; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Canis familiaris"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Ochotona princeps"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0045179	; C:apical cortex
GO:0016324	; C:apical plasma membrane
GO:0031252	; C:cell leading edge
GO:0005911	; C:cell-cell junction
GO:0005829	; C:cytosol
GO:0005768	; C:endosome
GO:0043231	; C:intracellular membrane-bounded organelle
GO:0045121	; C:membrane raft
GO:0035748	; C:myelin sheath abaxonal region
GO:0005635	; C:nuclear envelope
GO:0016363	; C:nuclear matrix
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005886	; C:plasma membrane
GO:0043234	; C:protein complex
GO:0005923	; C:tight junction
GO:0005524	; F:ATP binding
GO:0015459	; F:potassium channel regulator activity
GO:0004697	; F:protein kinase C activity
GO:0008270	; F:zinc ion binding
GO:0031532	; P:actin cytoskeleton reorganization
GO:0031584	; P:activation of phospholipase D activity
GO:0032148	; P:activation of protein kinase B activity
GO:0016477	; P:cell migration
GO:0034613	; P:cellular protein localization
GO:0030010	; P:establishment of cell polarity
GO:0006954	; P:inflammatory response
GO:0008286	; P:insulin receptor signaling pathway
GO:0007616	; P:long-term memory
GO:0060291	; P:long-term synaptic potentiation
GO:0060081	; P:membrane hyperpolarization
GO:0000226	; P:microtubule cytoskeleton organization
GO:0043066	; P:negative regulation of apoptotic process
GO:0051346	; P:negative regulation of hydrolase activity
GO:0046627	; P:negative regulation of insulin receptor signaling pathway
GO:0050732	; P:negative regulation of peptidyl-tyrosine phosphorylation
GO:0031333	; P:negative regulation of protein complex assembly
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0008284	; P:positive regulation of cell proliferation
GO:0001954	; P:positive regulation of cell-matrix adhesion
GO:0070374	; P:positive regulation of ERK1 and ERK2 cascade
GO:2000463	; P:positive regulation of excitatory postsynaptic membrane potential
GO:0046326	; P:positive regulation of glucose import
GO:0046628	; P:positive regulation of insulin receptor signaling pathway
GO:2001181	; P:positive regulation of interleukin-10 secretion
GO:2000667	; P:positive regulation of interleukin-13 secretion
GO:0032753	; P:positive regulation of interleukin-4 production
GO:2000664	; P:positive regulation of interleukin-5 secretion
GO:0051092	; P:positive regulation of NF-kappaB transcription factor activity
GO:2000553	; P:positive regulation of T-helper 2 cell cytokine production
GO:0045630	; P:positive regulation of T-helper 2 cell differentiation
GO:0051291	; P:protein heterooligomerization
GO:0070528	; P:protein kinase C signaling cascade
GO:0072659	; P:protein localization to plasma membrane
GO:0047496	; P:vesicle transport along microtubule

KEGG

rno:25522

;

InterPros

IPR000961	; AGC-kinase_C.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR000270	; OPR_PB1.
IPR012233	; PKC_zeta.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF00564	; PB1; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 1.
SM00666	; PB1; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013