EKS-RAN-00417

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00417

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	606.0	3.0E-182	599	957	359

Status

Reviewed

Ensembl Protein

ENSRNOP00000060534

UniProt Accession

Q05030; Q8R406; Q925F7;

Protein Name

Platelet-derived growth factor receptor beta

Protein Synonyms/Alias

PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1;

Gene Name

Pdgfrb

Gene Synonyms/Alias

Pdgfrb; Pdgfr, Pdgfr1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000018461	ENSRNOP00000060534	ENSRNOT00000068535

Organism

Rattus norvegicus

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor (By similarity).

Protein Length

1097

Protein Sequence
(FASTA)

MGLPEVMPAS VLRGQLLLFV LLLLGPQISQ GLVITPPGPE FVLNISSTFV LTCSSSAPVM 60

WEQMSQVPWQ EAAMNQDGTF SSVLTLTNVT GGDTGEYFCV YNNSLGPELS ERKRIYIFVP 120

DPTMGFLPMD SEDLFIFVTD VTETTIPCRV TDPQLEVTLH EKKVDIPLHV PYDHQRGFIG 180

TFEDKTYICK TTIGDREVDS DTYYVYSLQV SSINVSVNAV QTVVRQGESI TIRCIVMGND 240

VVNFQWTYPR MKSGRLVEPV TDYLFGVPSR IGSILHIPTA ELSDSGTYTC NVSVSVNDHG 300

DEKAINVTVI ENGYVRLLET LEDVQIAELH RSRTLQVVFE AYPTPSVLWF KDNRTLGDSS 360

AGELVLSTRN VSETRYVSEL TLVRVKVSEA GYYTMRAFHA DDQVQLSFKL QVNVPVRVLE 420

LSESHPANGE QILRCRGRGM PQPNVTWSTC RDLKRCPRKL SPTPLGNSSK EESQLETNVT 480

FWEEDQEYEV VSTLRLRHVD QPLSVRCMLQ NSMGRDSQEV TVVPHSLPFK VVVISAILAL 540

VVLTVISLII LIMLWQRKPR YEIRWKVIES VSSDGHEYIY VDPVQLPYDS TWELPRDQLV 600

LGRTLGSGAF GQVVEATAHG LSHSQATMKV AVKMLKSTAR SSEKQALMSE LKIMSHLGPH 660

LNVVNLLGAC TKGGPIYIIT EYCRYGDLVD YLHRNKHTFL QRHSNKHCPP STELYSNALP 720

VGLSLPSHLN LTGESDGGYM DMSKDESVDY VPMLDMKGHI KYADIESSSY MAPYDNYVPS 780

APERTYRATL INDSPVLSYT DLVGFSYQVA NGMEFLASKN CVHRDLAARN VLICEGKLVK 840

ICDFGLARDI MRDSNYISKG STFLPLKWMA PESIFNSLYT TLSDVWSFGI LLWEIFTLGG 900

TPYPELPMND QFYNAIKRGY RMAQPAHASD EIYEIMQKCW EEKFETRPPF SQLVLLLERL 960

LGEGYKKKYQ QVDEEFLRSD HPAILRSQAR LPGLHSLRSP LDTSSVLYTA VQPNETDNDY 1020

IIPLPDPKPD AADEGLLEGS PSLASSTLNE VNTSSTISCD SPLELQEEPQ AEPEAQLEQP 1080

QDSGCPGPLA EAEDSFL 1097

Nucleotide Sequence
(FASTA)

ATGGGGCTTC CAGAGGTGAT GCCAGCTTCA GTCCTCAGAG GCCAATTGTT GCTGTTCGTG 60

CTATTGCTCC TGGGACCACA GATCTCCCAA GGCCTAGTCA TCACGCCCCC TGGGCCAGAG 120

TTCGTCCTCA ACATTTCGAG CACCTTTGTT CTGACATGCT CAAGTTCAGC TCCAGTGATG 180

TGGGAACAGA TGTCCCAGGT GCCCTGGCAA GAAGCAGCCA TGAACCAGGA TGGCACCTTC 240

TCCAGTGTGC TGACACTGAC TAATGTCACT GGGGGAGACA CCGGAGAATA CTTTTGTGTC 300

TACAACAACT CACTGGGGCC AGAGCTCAGT GAGCGGAAGC GCATCTATAT CTTTGTGCCA 360

GATCCCACCA TGGGCTTCCT CCCGATGGAC TCTGAGGACC TATTCATTTT TGTCACGGAT 420

GTCACTGAGA CGACGATTCC ATGCCGAGTG ACAGACCCCC AGCTGGAGGT GACGCTGCAT 480

GAGAAGAAAG TGGATATCCC CCTACATGTA CCCTATGACC ACCAGCGAGG TTTCATTGGT 540

ACTTTTGAGG ACAAGACTTA CATTTGCAAA ACCACCATTG GGGACAGGGA AGTGGACTCC 600

GATACTTACT ATGTCTACAG CCTGCAGGTG TCATCCATCA ATGTCTCTGT GAATGCAGTG 660

CAGACTGTGG TCCGCCAGGG CGAGAGCATC ACCATCAGGT GCATCGTGAT GGGCAATGAT 720

GTGGTCAACT TCCAGTGGAC GTACCCCCGC ATGAAGAGTG GGCGGCTGGT GGAGCCGGTG 780

ACAGATTACC TCTTCGGAGT GCCCTCCCGC ATTGGCTCCA TTCTTCATAT CCCCACCGCT 840

GAGCTGAGTG ACTCTGGCAC CTATACTTGC AACGTGTCAG TGAGTGTGAA TGACCACGGC 900

GATGAGAAAG CCATCAACGT TACTGTGATC GAAAATGGCT ATGTGCGGCT GCTGGAAACA 960

CTGGAAGACG TACAAATTGC TGAGCTGCAC CGGAGCCGGA CGTTGCAGGT GGTGTTTGAG 1020

GCTTATCCGA CGCCCTCTGT CCTGTGGTTC AAGGACAACC GTACCTTGGG TGACTCCAGC 1080

GCTGGCGAGT TGGTTCTGTC CACTCGCAAC GTGTCTGAGA CCCGGTATGT GTCAGAACTG 1140

ACCTTGGTAC GTGTGAAGGT GTCAGAAGCG GGCTACTATA CCATGCGGGC CTTCCATGCG 1200

GACGACCAGG TCCAGCTCTC ATTCAAGCTG CAGGTCAATG TCCCTGTCCG TGTACTGGAG 1260

CTGAGTGAGA GTCATCCTGC CAATGGGGAG CAGATACTCC GCTGTCGGGG CCGGGGCATG 1320

CCTCAGCCAA ATGTCACCTG GTCTACCTGC AGAGACCTCA AAAGGTGTCC GCGAAAACTG 1380

TCACCCACAC CCTTGGGGAA TAGTTCCAAG GAGGAGAGCC AGCTAGAAAC GAACGTGACT 1440

TTCTGGGAGG AAGATCAGGA ATATGAGGTG GTGAGCACGC TGCGCCTGCG TCACGTGGAC 1500

CAGCCACTGT CAGTGCGCTG TATGCTGCAG AACTCGATGG GCAGAGATTC ACAAGAGGTC 1560

ACCGTGGTAC CACACTCCTT GCCTTTCAAA GTGGTGGTGA TCTCAGCCAT CTTGGCCTTG 1620

GTGGTCCTTA CTGTCATCTC TCTCATCATC CTCATCATGC TGTGGCAGAG GAAACCACGC 1680

TATGAGATCC GATGGAAGGT CATTGAGTCT GTGAGCTCTG ACGGCCATGA GTACATCTAT 1740

GTGGATCCTG TGCAGTTGCC TTACGACTCC ACCTGGGAGC TGCCACGGGA CCAGCTTGTT 1800

CTGGGACGCA CTCTTGGCTC TGGGGCTTTC GGACAGGTGG TGGAGGCCAC GGCTCATGGT 1860

CTGAGCCACT CACAGGCCAC CATGAAAGTG GCTGTCAAGA TGCTGAAATC TACAGCCAGA 1920

AGCAGTGAGA AGCAAGCCCT CATGTCGGAG CTGAAGATCA TGAGTCATCT CGGACCCCAC 1980

CTGAACGTGG TCAACCTGCT GGGGGCCTGT ACCAAAGGAG GGCCCATCTA CATTATCACG 2040

GAATACTGCC GTTATGGTGA CCTGGTGGAC TACCTGCACC GAAACAAACA CACCTTCCTG 2100

CAACGACACT CCAACAAGCA TTGTCCACCC AGCACTGAGC TCTACAGCAA CGCCCTGCCG 2160

GTGGGGCTCT CCCTACCCAG CCACTTGAAC CTGACTGGGG AGAGCGACGG TGGTTACATG 2220

GACATGAGCA AGGATGAATC TGTAGATTAC GTGCCCATGT TGGACATGAA AGGACACATC 2280

AAATACGCGG ACATCGAGTC CTCCAGCTAC ATGGCCCCTT ACGATAACTA TGTCCCATCT 2340

GCCCCTGAAA GGACCTATCG TGCTACCTTA ATCAACGACT CACCAGTGCT CAGCTACACG 2400

GACCTCGTGG GCTTCAGTTA CCAAGTGGCC AATGGCATGG AATTCTTGGC CTCTAAGAAC 2460

TGTGTTCACC GAGACCTGGC GGCCAGGAAT GTGCTCATCT GTGAGGGCAA GTTGGTCAAG 2520

ATCTGTGACT TTGGCCTGGC TCGAGACATC ATGCGGGACT CAAACTACAT CTCCAAAGGC 2580

AGTACTTTCC TGCCTCTGAA GTGGATGGCC CCAGAGAGCA TCTTCAACAG CCTCTACACC 2640

ACCTTGAGTG ATGTGTGGTC CTTTGGAATC CTACTCTGGG AAATCTTCAC ACTGGGTGGC 2700

ACCCCTTACC CAGAGCTGCC CATGAACGAC CAGTTCTACA ATGCCATCAA GAGGGGCTAC 2760

CGCATGGCCC AACCTGCTCA TGCCTCCGAC GAGATCTATG AGATCATGCA GAAATGCTGG 2820

GAAGAAAAGT TTGAGACTCG GCCCCCCTTC TCTCAGCTGG TGCTGCTCCT GGAGAGGCTT 2880

CTGGGTGAAG GCTATAAAAA GAAGTACCAG CAGGTGGATG AGGAGTTTCT GAGGAGTGAC 2940

CATCCAGCCA TCCTGAGGTC CCAAGCCCGC TTGCCGGGTC TCCACAGCCT CCGATCCCCT 3000

CTGGATACCA GCTCTGTCCT CTACACTGCT GTGCAGCCTA ATGAGACTGA CAATGACTAC 3060

ATCATCCCCT TGCCTGACCC CAAGCCTGAT GCTGCTGATG AGGGCCTCCT AGAGGGTTCC 3120

CCCAGCCTTG CCAGCTCCAC CTTGAATGAA GTCAACACTT CTTCCACCAT CTCCTGCGAC 3180

AGCCCCCTGG AGCTCCAAGA AGAGCCGCAA GCAGAGCCTG AGGCACAGCT GGAGCAGCCA 3240

CAGGATTCAG GCTGCCCAGG ACCTCTGGCT GAGGCAGAGG ACAGCTTCCT GTAG 3294

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l+lG++lGsGafG+vveata+Gls+s+a++kvavkmlkstarssek+almselki+shlG

Q: 599   LVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLG  658

         78**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+n+vnllGactkgGpiy+iteyc+yGdl++yl+r++++fl+++++k++++++el+s+a

Q: 659   PHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQRHSNKHCPPSTELYSNA  718

         ************************************************************

S: 121   lladkslrsksslssesseeyvdlkkd................vsdvssskadklasekk  164

         l+++ sl s+ +l+ es++ y+d++kd                ++d++ss++++++++++

Q: 719   LPVGLSLPSHLNLTGESDGGYMDMSKDesvdyvpmldmkghikYADIESSSYMAPYDNYV  778

         ************************************************************

S: 165   ksalerevkaaleeedsevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGk  224

          sa er+++a+l+++ s vl+++dl+ fsyqva+Gmeflaskncvhrdlaarnvl++eGk

Q: 779   PSAPERTYRATLIND-SPVLSYTDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGK  837

         *************98.9*******************************************

S: 225   vvkicdfGlardimkdsnyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifs  284

         +vkicdfGlardim+dsny++kG+++lp+kwmapesif+slyt+ sdvws+Gillweif+

Q: 838   LVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFT  897

         ************************************************************

S: 285   lGstpypgllvdskfyklikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         lG+tpyp+l+++++fy++ik+Gyrmaqpa+a++eiyeimqkcwe+++e+rp+f+qlv ll

Q: 898   LGGTPYPELPMNDQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFETRPPFSQLVLLL  957

         ********************************************************9986

Domain Sequence
(FASTA)

LVLGRTLGSG AFGQVVEATA HGLSHSQATM KVAVKMLKST ARSSEKQALM SELKIMSHLG 60

PHLNVVNLLG ACTKGGPIYI ITEYCRYGDL VDYLHRNKHT FLQRHSNKHC PPSTELYSNA 120

LPVGLSLPSH LNLTGESDGG YMDMSKDESV DYVPMLDMKG HIKYADIESS SYMAPYDNYV 180

PSAPERTYRA TLINDSPVLS YTDLVGFSYQ VANGMEFLAS KNCVHRDLAA RNVLICEGKL 240

VKICDFGLAR DIMRDSNYIS KGSTFLPLKW MAPESIFNSL YTTLSDVWSF GILLWEIFTL 300

GGTPYPELPM NDQFYNAIKR GYRMAQPAHA SDEIYEIMQK CWEEKFETRP PFSQLVLLL 359

Keyword

ATP-binding; Cell membrane; Chemotaxis; Complete proteome; Cytoplasmic vesicle; Developmental protein; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Lysosome; Membrane; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016324	; C:apical plasma membrane
GO:0005737	; C:cytoplasm
GO:0016023	; C:cytoplasmic membrane-bounded vesicle
GO:0005887	; C:integral to plasma membrane
GO:0043202	; C:lysosomal lumen
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0005019	; F:platelet-derived growth factor beta-receptor activity
GO:0005021	; F:vascular endothelial growth factor-activated receptor activity
GO:0055003	; P:cardiac myofibril assembly
GO:0060326	; P:cell chemotaxis
GO:0060981	; P:cell migration involved in coronary angiogenesis
GO:0035441	; P:cell migration involved in vasculogenesis
GO:0006024	; P:glycosaminoglycan biosynthetic process
GO:0048839	; P:inner ear development
GO:0007243	; P:intracellular protein kinase cascade
GO:0072278	; P:metanephric comma-shaped body morphogenesis
GO:0072277	; P:metanephric glomerular capillary formation
GO:0072262	; P:metanephric glomerular mesangial cell proliferation involved in metanephros development
GO:0035789	; P:metanephric mesenchymal cell migration
GO:0072075	; P:metanephric mesenchyme development
GO:0072284	; P:metanephric S-shaped body morphogenesis
GO:0043066	; P:negative regulation of apoptotic process
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0046488	; P:phosphatidylinositol metabolic process
GO:0048015	; P:phosphatidylinositol-mediated signaling
GO:0090280	; P:positive regulation of calcium ion import
GO:0050921	; P:positive regulation of chemotaxis
GO:0032967	; P:positive regulation of collagen biosynthetic process
GO:2000573	; P:positive regulation of DNA biosynthetic process
GO:0070374	; P:positive regulation of ERK1 and ERK2 cascade
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0035793	; P:positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway
GO:0045840	; P:positive regulation of mitosis
GO:0043552	; P:positive regulation of phosphatidylinositol 3-kinase activity
GO:0014068	; P:positive regulation of phosphatidylinositol 3-kinase cascade
GO:0010863	; P:positive regulation of phospholipase C activity
GO:2000379	; P:positive regulation of reactive oxygen species metabolic process
GO:0014911	; P:positive regulation of smooth muscle cell migration
GO:0048661	; P:positive regulation of smooth muscle cell proliferation
GO:0046777	; P:protein autophosphorylation
GO:0032355	; P:response to estradiol stimulus
GO:0034405	; P:response to fluid shear stress
GO:0042542	; P:response to hydrogen peroxide
GO:0055093	; P:response to hyperoxia
GO:0032526	; P:response to retinoic acid
GO:0009636	; P:response to toxin
GO:0061298	; P:retina vasculature development in camera-type eye
GO:0007179	; P:transforming growth factor beta receptor signaling pathway
GO:0048010	; P:vascular endothelial growth factor receptor signaling pathway
GO:0038084	; P:vascular endothelial growth factor signaling pathway
GO:0042060	; P:wound healing

KEGG

rno:24629

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR013151	; Immunoglobulin.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.
IPR009134	; Tyr_kinase_VEGFR_rcpt_N.

Pfam

PF07679	; I-set; 1.
PF00047	; ig; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 2.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 3.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

PR01832

; VEGFRECEPTOR.

Created Date

20-Feb-2013