EKS-RAN-00277

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00277

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	415.0	1.5E-124	62	314	253

Status

Reviewed

Ensembl Protein

ENSRNOP00000025197

UniProt Accession

Q9R011;

Protein Name

Serine/threonine-protein kinase PLK3

Protein Synonyms/Alias

Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3;

Gene Name

Plk3

Gene Synonyms/Alias

Plk3; Cnk, Fnk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000018484	ENSRNOP00000025197	ENSRNOT00000025197

Organism

Rattus norvegicus

Functional Description

Serine/threonine-protein kinase involved in cell cycleregulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1 (By similarity).

Protein Length

647

Protein Sequence
(FASTA)

MEPAAGFLSP RPFPRAAAPS SPPAGPGPPA SASPRSEPGV LAGPQTPDAS RLITDPRSGR 60

TYIKGRLLGK GGFARCYEAT DTETSIAYAV KVIPQSRVAK PHQREKIINE IELHRDLQHR 120

HIVRFSHHFE DADNIYIFLE LCSRKSLAHI WKARHTLLEP EVRYYLRQIL SGLKYLHQRG 180

ILHRDLKLGN FFITDNMELK VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPE 240

ADVWSLGCVM YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPR 300

DRPSIEQILR HDFFTKGYTP DRLPVSSCVT VPDLTPPNPA RSLFAKVTKS LFGRRKSKNK 360

NHSEEQDNVS CLVSGLMRTS IGHPDVRPEA PAASALAPVS LVETAAEDSS PRGTLASSGD 420

GFEEGLTVTT VVESALCALR NCVAFMPPAE QNPAPLAQPE PLVWVSKWVD YSNKFGFGYQ 480

LSSRRVAVLF NDGTHMALSA NRKTVHYNPT STKHFSFSVG SVPRALQPQL GILRYFASYM 540

EQHLMKGGDL PSVEEVEVPA PPLLLQWVKT DQALLMLFSD GTVQVNFYGD HTKLILSGWE 600

PLLVTFVARN RSACTYLASH LRQLGCSPDL RQRLRYALRL LRDRSPA 647

Nucleotide Sequence
(FASTA)

ATGGAGCCCG CCGCCGGCTT CTTGTCCCCG CGCCCCTTCC CGCGCGCGGC TGCTCCGTCC 60

TCGCCCCCCG CCGGTCCGGG GCCGCCCGCC AGTGCCTCCC CACGGTCGGA ACCTGGGGTG 120

CTGGCCGGGC CACAGACGCC GGATGCCAGT CGCCTCATCA CCGACCCTCG CAGCGGCCGT 180

ACCTACATCA AGGGTCGCTT GTTGGGCAAG GGGGGCTTCG CTCGCTGCTA TGAAGCCACT 240

GACACTGAGA CTAGTATAGC CTACGCGGTC AAAGTCATCC CGCAGAGTCG CGTCGCCAAG 300

CCGCATCAGC GCGAGAAGAT CATAAACGAG ATAGAGTTGC ACCGAGACCT ACAGCACCGC 360

CATATCGTTC GATTTTCGCA TCATTTCGAG GATGCTGACA ACATATATAT TTTCCTGGAG 420

CTCTGTAGCC GAAAGTCCCT GGCCCACATC TGGAAGGCCC GTCACACCCT ACTGGAACCA 480

GAGGTTCGCT ACTACCTGCG CCAGATTCTT TCTGGTCTCA AGTACTTGCA CCAGAGGGGT 540

ATCTTGCACA GAGATCTCAA GCTGGGAAAT TTTTTCATCA CTGATAACAT GGAACTGAAG 600

GTGGGAGATT TTGGGCTGGC AGCTCGGCTA GAGCCTCCAG AGCAGAGGAA AAAGACCATC 660

TGTGGTACTC CCAACTATGT GGCCCCAGAA GTGCTGCTGA GGCAGGGCCA TGGCCCCGAG 720

GCAGATGTGT GGTCCCTTGG TTGTGTCATG TACACACTCC TGTGTGGGAG CCCCCCGTTT 780

GAGACAGCTG ACCTAAAGGA GACCTATCGC TGCATCAAGC AGGTTCATTA CACACTGCCT 840

GCCAGCCTCT CACTGCCTGC CCGCCAGCTC CTGGCTGCCA TCCTTCGAGC TTCACCCCGA 900

GACCGCCCTT CCATTGAGCA GATCCTGCGC CATGACTTCT TTACAAAGGG CTACACCCCT 960

GATCGGCTCC CTGTCAGCAG CTGTGTGACA GTCCCAGATC TGACACCCCC CAACCCTGCG 1020

AGGAGTCTGT TTGCTAAAGT TACCAAGAGC CTGTTTGGCA GGAGAAAGAG CAAGAATAAG 1080

AACCATTCTG AGGAACAGGA TAATGTCTCC TGCTTGGTGA GCGGCCTCAT GCGCACATCC 1140

ATCGGTCATC CAGATGTCAG GCCGGAGGCT CCAGCAGCCT CAGCTCTGGC CCCTGTCAGC 1200

TTGGTAGAGA CAGCTGCTGA GGACAGCTCA CCCCGTGGGA CACTGGCAAG CAGTGGAGAT 1260

GGATTTGAAG AAGGTTTGAC TGTGACCACG GTGGTGGAGT CTGCCCTCTG TGCACTGAGA 1320

AACTGTGTGG CCTTCATGCC CCCAGCTGAA CAGAACCCAG CCCCGTTGGC ACAGCCAGAG 1380

CCTCTGGTGT GGGTCAGCAA GTGGGTTGAC TACTCCAACA AGTTTGGCTT TGGCTATCAG 1440

CTGTCCAGCC GCCGTGTAGC CGTGCTCTTC AATGACGGCA CACACATGGC CCTGTCAGCC 1500

AATAGGAAGA CTGTGCACTA CAACCCCACC AGCACGAAGC ACTTCTCTTT CTCTGTGGGT 1560

TCTGTGCCCC GTGCTCTGCA GCCTCAGCTG GGCATCCTCC GGTATTTCGC CTCCTACATG 1620

GAGCAGCACC TCATGAAGGG TGGAGATCTG CCCAGTGTGG AGGAGGTAGA GGTCCCTGCC 1680

CCACCCTTGC TGCTGCAGTG GGTCAAGACG GACCAGGCCC TTCTCATGCT GTTCAGTGAC 1740

GGCACTGTCC AGGTGAACTT CTACGGGGAC CATACGAAGC TGATCCTCAG TGGCTGGGAG 1800

CCTCTCCTCG TGACCTTCGT GGCCCGAAAT CGCAGTGCTT GTACTTACCT CGCCTCCCAC 1860

CTCCGGCAGC TGGGCTGCTC CCCAGACTTG CGGCAGCGAC TGCGCTATGC TCTGCGCCTG 1920

CTCAGGGACC GAAGCCCTGC TTAG 1944

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y +g+llGkGgfa+cye++d+et+ ++avkv+p+s++ak++qrek+ +eie+hr+l+h++

Q: 62    YIKGRLLGKGGFARCYEATDTETSIAYAVKVIPQSRVAKPHQREKIINEIELHRDLQHRH  121

         889*********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         iv+++++fed++n+y++lelcsr+sla+++k+r++l epevryylrqi+sglkylh++ i

Q: 122   IVRFSHHFEDADNIYIFLELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGI  181

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         lhrdlklgn+f+++++e+k+gdfGla+rle++e+rkkt+cGtPny+aPevl ++gh+ e+

Q: 182   LHRDLKLGNFFITDNMELKVGDFGLAARLEPPEQRKKTICGTPNYVAPEVLLRQGHGPEA  241

         ************************************************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         d+WslGc++ytll+G+PPfet++lkety++ik+ +y+lP+sls +a++l+a++l+++P++

Q: 242   DVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLPASLSLPARQLLAAILRASPRD  301

         ************************************************************

S: 241   rpsleevlkdefl  253

         rps+e++l+++f+

Q: 302   RPSIEQILRHDFF  314

         ************8

Domain Sequence
(FASTA)

YIKGRLLGKG GFARCYEATD TETSIAYAVK VIPQSRVAKP HQREKIINEI ELHRDLQHRH 60

IVRFSHHFED ADNIYIFLEL CSRKSLAHIW KARHTLLEPE VRYYLRQILS GLKYLHQRGI 120

LHRDLKLGNF FITDNMELKV GDFGLAARLE PPEQRKKTIC GTPNYVAPEV LLRQGHGPEA 180

DVWSLGCVMY TLLCGSPPFE TADLKETYRC IKQVHYTLPA SLSLPARQLL AAILRASPRD 240

RPSIEQILRH DFF 253

Keyword

Apoptosis; ATP-binding; Cell cycle; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Golgi apparatus; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0030425	; C:dendrite
GO:0005795	; C:Golgi stack
GO:0043025	; C:neuronal cell body
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0002039	; F:p53 binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0071779	; P:G1/S transition checkpoint
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0090166	; P:Golgi disassembly
GO:0043066	; P:negative regulation of apoptotic process
GO:0000122	; P:negative regulation of transcription from RNA polymerase II promoter
GO:2000777	; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxia
GO:0043491	; P:protein kinase B signaling cascade
GO:0048169	; P:regulation of long-term neuronal synaptic plasticity
GO:0006974	; P:response to DNA damage stimulus
GO:0006970	; P:response to osmotic stress
GO:0009314	; P:response to radiation
GO:0000302	; P:response to reactive oxygen species
GO:0000084	; P:S phase of mitotic cell cycle

KEGG

rno:58936

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR020658	; Ser/Thr_kinase_Plk3.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013