EKS-RAN-00463

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00463

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/PDHK	188.2	7.6E-56	264	400	137

Status

Reviewed

Ensembl Protein

ENSRNOP00000026466

UniProt Accession

Q00972; Q64552;

Protein Name

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

Protein Synonyms/Alias

Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN;

Gene Name

Bckdk

Gene Synonyms/Alias

Bckdk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000019485	ENSRNOP00000026466	ENSRNOT00000026466

Organism

Rattus norvegicus

Functional Description

Catalyzes the phosphorylation and inactivation of thebranched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Protein Length

412

Protein Sequence
(FASTA)

MILTSVLGSG PRSGSSLWPL LGSSLSLRVR STSATDTHHV ELARERSKTV TSFYNQSAID 60

VVAEKPSVRL TPTMMLYSGR SQDGSHLLKS GRYLQQELPV RIAHRIKGFR SLPFIIGCNP 120

TILHVHELYI RAFQKLTDFP PIKDQADEAQ YCQLVRQLLD DHKDVVTLLA EGLRESRKHI 180

EDEKLVRYFL DKTLTSRLGI RMLATHHLAL HEDKPDFVGI ICTRLSPKKI IEKWVDFARR 240

LCEHKYGNAP RVRINGHVAA RFPFIPMPLD YILPELLKNA MRATMESHLD TPYNVPDVVI 300

TIANNDVDLI IRISDRGGGI AHKDLDRVMD YHFTTAEAST QDPRISPLFG HLDMHSGGQS 360

GPMHGFGFGL PTSRAYAEYL GGSLQLQSLQ GIGTDVYLRL RHIDGREESF RI 412

Nucleotide Sequence
(FASTA)

ATGATACTGA CTTCAGTGCT GGGCAGCGGC CCTCGGAGCG GGTCTTCACT TTGGCCCCTC 60

TTGGGGTCCT CACTGTCACT CCGGGTTCGC TCAACATCAG CCACCGATAC ACACCATGTA 120

GAGCTGGCCA GGGAACGCTC CAAGACTGTT ACCTCCTTTT ACAACCAGTC AGCTATTGAC 180

GTGGTAGCAG AGAAGCCCTC AGTCCGCCTC ACTCCCACCA TGATGCTCTA TTCTGGTCGC 240

TCACAGGATG GCAGCCACCT TCTGAAAAGT GGTCGCTACT TGCAGCAAGA GTTACCGGTG 300

AGGATCGCTC ACCGCATCAA GGGCTTCCGT AGTCTTCCTT TCATCATTGG TTGCAACCCT 360

ACCATACTGC ATGTGCACGA GCTATACATC CGGGCCTTCC AGAAGTTGAC AGACTTCCCT 420

CCGATCAAGG ACCAGGCAGA CGAAGCCCAG TATTGCCAGC TGGTGCGACA GCTGCTAGAT 480

GACCACAAGG ATGTGGTAAC CCTGTTAGCT GAGGGTCTGC GTGAGAGCCG GAAACACATA 540

GAGGATGAAA AGCTGGTCCG CTACTTCCTG GATAAAACAC TAACGTCCAG ACTTGGGATC 600

CGAATGCTGG CTACTCACCA CTTGGCGCTA CATGAAGACA AGCCTGATTT TGTTGGCATC 660

ATCTGCACTC GTCTGTCACC CAAGAAGATT ATTGAGAAGT GGGTGGATTT TGCCAGACGC 720

CTGTGCGAGC ACAAGTATGG CAATGCCCCT AGAGTCCGCA TCAATGGGCA CGTGGCTGCC 780

CGTTTCCCCT TCATTCCTAT GCCGCTGGAC TATATCCTGC CTGAGCTGCT CAAGAACGCC 840

ATGAGAGCCA CAATGGAGAG TCACCTAGAC ACGCCCTACA ATGTTCCTGA TGTGGTCATC 900

ACCATCGCCA ATAACGATGT GGATCTCATC ATCAGGATCT CAGACCGGGG TGGAGGAATC 960

GCTCATAAGG ACCTGGATCG GGTCATGGAC TACCACTTCA CCACAGCTGA GGCCAGCACC 1020

CAGGACCCCC GAATCAGCCC CCTCTTCGGC CACCTGGATA TGCACAGTGG TGGCCAATCA 1080

GGACCTATGC ATGGCTTTGG CTTCGGGTTG CCCACGTCCA GGGCCTATGC GGAGTATCTC 1140

GGTGGCTCCC TGCAGCTGCA GTCCCTGCAG GGCATTGGCA CAGATGTCTA CCTACGGCTC 1200

CGCCACATTG ATGGCCGGGA GGAAAGCTTC AGAATCTGA 1239

Domain Profile

S: 1     yvpshlyyllfellknamratveshedkkv.lpdvkvlvalgkedliikisdrGGGvplr  59

         ++p++l+y+l+ellknamrat+esh d+++ +pdv++++a++++dlii+isdrGGG++++

Q: 264   FIPMPLDYILPELLKNAMRATMESHLDTPYnVPDVVITIANNDVDLIIRISDRGGGIAHK  323

         8*****************************9*****************************

S: 60    kidrlldymystapapskdd..............araaplaGfGyGlpisrlyakylqGd  105

         ++dr++dy+++ta+a+++d+               +++p++GfG+Glp+sr+ya+yl+G+

Q: 324   DLDRVMDYHFTTAEASTQDPrisplfghldmhsgGQSGPMHGFGFGLPTSRAYAEYLGGS  383

         *********************************99*************************

S: 106   lkllsleGiGtdaviylka  124

         l+l+sl+GiGtd+  yl++

Q: 384   LQLQSLQGIGTDV--YLRL  400

         ************9..8886

Domain Sequence
(FASTA)

FIPMPLDYIL PELLKNAMRA TMESHLDTPY NVPDVVITIA NNDVDLIIRI SDRGGGIAHK 60

DLDRVMDYHF TTAEASTQDP RISPLFGHLD MHSGGQSGPM HGFGFGLPTS RAYAEYLGGS 120

LQLQSLQGIG TDVYLRL 137

Keyword

3D-structure; Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005947	; C:mitochondrial alpha-ketoglutarate dehydrogenase complex
GO:0047323	; F:[3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0009083	; P:branched-chain amino acid catabolic process

KEGG

rno:29603

;

InterPros

IPR003594	; ATPase-like_ATP-bd.
IPR018955	; BCDHK/PDK_N.
IPR004358	; Sig_transdc_His_kin-like_C.
IPR005467	; Sig_transdc_His_kinase_core.

Pfam

PF10436	; BCDHK_Adom3; 1.
PF02518	; HATPase_c; 1.

SMARTs

SM00387

; HATPase_c; 1.

Prosites

PS50109

; HIS_KIN; 1.

Prints

PR00344

; BCTRLSENSOR.

Created Date

20-Feb-2013