EKS-RAN-00247

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00247

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	321.0	5.7E-96	2	241	240

Status

Reviewed

Ensembl Protein

ENSRNOP00000027134

UniProt Accession

D3ZML2; E9PTC7; Q4A1P4;

Protein Name

Serine/threonine-protein kinase BRSK2

Protein Synonyms/Alias

Brain-specific serine/threonine-protein kinase 2; BR serine/threonine-protein kinase 2; Serine/threonine-protein kinase SAD-A;

Gene Name

Brsk2

Gene Synonyms/Alias

Brsk2; Sada;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000020021	ENSRNOP00000027134	ENSRNOT00000027134
ENSRNOG00000020021	ENSRNOP00000051751	ENSRNOT00000054868

Organism

Rattus norvegicus

Functional Description

Serine/threonine-protein kinase that plays a key role inpolarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-523' and 'Ser-573'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion, BRSK2 phosphorylated at Thr-261 can promote insulin secretion. Regulates reorganization of the actin cytoskeleton. May play a role in the apopototic response triggered by endoplasmatic reticulum (ER) stress (By similarity).

Protein Length

705

Protein Sequence
(FASTA)

TGLVKLGIHC VTCQKVAIKI VNREKLSESV LMKVEREIAI LKLIEHPHVL KLHDVYENKK 60

YLYLVLEHVS GGELFDYLVK KGRLTPKEAR KFFRQIISAL DFCHSHSICH RDLKPENLLL 120

DERNNIRIAD FGMASLQVGD SLLETSCGSP HYACPEVIRG EKYDGRKADV WSCGVILFAL 180

LVGALPFDDD NLRQLLEKVK RGVFHMPHFI PPDCQSLLRG MIEVDAARRL TLEHIQKHIW 240

YIGGKNEPEP EQPIPRKVQI RSLPSLEDID PDVLDSMHSL GCFRDRNKLL QDLLSEEENQ 300

EKMIYFLLLD RKERYPSHED EDLPPRNEID PPRKRVDSPM LNRHGKRRPE RKSMEVLSVT 360

DGGSPVPARR AIEMAQHGQR SRSISGASSG LSTSPLSSPR VTPHPSPRGS PLPTPKGTPV 420

HTPKESPAGT PNPTPPSSPS VGGVPWRTRL NSIKNSFLGS PRFHRRKLQV PTPEEMSNLT 480

PESSPELGHL QLFGNPVSKV RSVAMELVIL VQTLAYTSFR LLGTFLPVRY LRHSVLSRPT 540

EQAGPSTPGR HCTSVNLVWN MVGMAYTQNP PIVGETGVYG SQWVMSSAPS KHYTSLGLSV 600

PSPSCSLSPS LFPFCAPDTT NCMEVMTGRL SKCGTPLSNF FDVIKQLFSD EKNGQAAQAP 660

STPAKRSAHG PLGDSAAAGP GGDTEYPMGK DMAKMGPPAA RREQP 705

Nucleotide Sequence
(FASTA)

ACAGGCTTGG TGAAACTGGG AATCCACTGT GTCACCTGCC AGAAGGTCGC CATCAAAATC 60

GTCAACCGTG AGAAGCTCAG TGAGTCGGTG CTGATGAAGG TGGAGCGAGA GATTGCCATC 120

TTGAAGCTCA TCGAACATCC ACATGTACTG AAGCTGCATG ACGTCTATGA AAACAAAAAA 180

TATTTATACC TGGTGCTAGA ACATGTGTCT GGGGGAGAGC TGTTCGACTA CCTGGTGAAG 240

AAGGGCCGGC TGACCCCCAA GGAGGCCCGC AAGTTCTTCC GGCAGATCAT CTCTGCACTG 300

GACTTCTGCC ACAGCCACTC CATATGCCAT AGAGACTTGA AGCCAGAGAA CCTGCTGCTG 360

GATGAGAGGA ACAACATCCG TATTGCAGAC TTTGGCATGG CGTCCCTGCA GGTGGGAGAC 420

AGCCTGCTGG AGACCAGCTG CGGATCTCCA CACTATGCCT GTCCGGAAGT GATTCGGGGC 480

GAGAAGTATG ATGGCCGCAA GGCAGATGTG TGGAGCTGTG GTGTGATCCT GTTCGCCTTG 540

CTGGTGGGGG CTCTGCCGTT TGATGATGAC AACCTGCGGC AGTTGCTGGA GAAGGTCAAG 600

CGTGGTGTGT TCCACATGCC ACACTTCATC CCACCAGACT GCCAGAGCCT CCTGCGTGGC 660

ATGATTGAGG TGGATGCAGC TCGGCGCCTC ACGCTAGAGC ACATTCAGAA ACACATATGG 720

TATATAGGTG GCAAGAATGA GCCAGAGCCG GAACAGCCCA TCCCACGCAA GGTGCAGATC 780

CGCTCACTGC CCAGCCTGGA AGACATTGAC CCTGATGTAC TGGACAGCAT GCATTCACTG 840

GGCTGCTTCC GAGACCGCAA CAAGCTGCTG CAGGACCTGC TATCTGAGGA GGAGAATCAG 900

GAAAAGATGA TTTATTTCCT CCTCCTGGAC CGGAAAGAAC GGTACCCCAG CCATGAGGAT 960

GAGGACCTGC CCCCCAGGAA TGAGATAGAC CCTCCTCGGA AGCGCGTGGA CTCCCCGATG 1020

CTGAACCGGC ATGGCAAGAG GCGACCTGAG CGCAAGTCCA TGGAGGTGCT CAGTGTGACA 1080

GATGGCGGCT CCCCAGTGCC TGCACGGAGA GCCATTGAGA TGGCCCAGCA TGGCCAGAGA 1140

TCTCGATCCA TCAGTGGTGC CTCCTCAGGC CTTTCTACAA GTCCACTCAG CAGTCCTCGG 1200

GTGACCCCTC ACCCCTCACC AAGGGGTAGT CCCCTTCCTA CCCCCAAAGG GACGCCTGTT 1260

CACACGCCAA AGGAGAGCCC AGCTGGCACA CCCAACCCCA CACCACCGTC CAGCCCTAGT 1320

GTTGGAGGAG TGCCCTGGCG GACACGACTC AACTCCATCA AGAACAGCTT CCTGGGCTCA 1380

CCTCGCTTCC ACCGCCGGAA ACTGCAAGTT CCCACGCCAG AGGAGATGTC CAACCTGACC 1440

CCAGAATCTT CTCCAGAGCT GGGGCATCTG CAGCTCTTCG GGAATCCCGT CAGCAAAGTG 1500

AGGAGTGTTG CAATGGAACT TGTTATACTG GTCCAGACTC TAGCATACAC CAGCTTCCGG 1560

TTGTTGGGAA CATTTCTTCC AGTAAGGTAC TTAAGACACT CTGTCCTGTC CCGTCCTACA 1620

GAGCAAGCAG GGCCAAGTAC CCCTGGGAGG CACTGTACTT CAGTCAACCT TGTCTGGAAT 1680

ATGGTGGGTA TGGCCTATAC ACAGAACCCA CCCATAGTGG GAGAGACAGG GGTGTATGGA 1740

AGCCAGTGGG TAATGAGTTC TGCCCCTTCC AAACACTACA CTTCCCTAGG CCTGTCTGTA 1800

CCATCACCCT CCTGTTCACT CTCTCCCTCC CTCTTTCCAT TCTGTGCTCC AGACACCACT 1860

AACTGTATGG AAGTGATGAC GGGGCGGCTT TCCAAATGTG GCACCCCATT GAGTAACTTC 1920

TTTGACGTAA TTAAACAACT TTTTTCAGAC GAGAAGAACG GGCAGGCGGC CCAGGCCCCC 1980

AGCACACCCG CCAAGCGGAG TGCCCACGGC CCCCTGGGTG ACTCCGCGGC CGCTGGCCCT 2040

GGAGGGGACA CCGAGTACCC GATGGGCAAG GACATGGCCA AGATGGGGCC GCCCGCCGCC 2100

CGCCGTGAGC AGCCTTAG 2118

Domain Profile

S: 13    gkVklakhketkekvavKiikkkklseeslekikrEieilkklkhpniikllevietkkk  72

         g Vkl +h  t +kva+Ki++++klse+ l k++rEi+ilk ++hp+++kl++v+e+kk+

Q: 2     GLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKY  61

         78**********************************************************

S: 73    lylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgivHrDlKpeNllld  132

         lylvle++sggeLfdy+++kgrl+ keark+f+q++sa++++hs++i+HrDlKpeNllld

Q: 62    LYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLD  121

         ************************************************************

S: 133   kkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkvDvWslGvvLyalv  192

         +++ni+iaDFG+a+l   ++llet+cGsp+Ya+Pe+++g+kY+g+k+DvWs+Gv+L+al+

Q: 122   ERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALL  181

         ************************************************************

S: 193   tgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpekRatieeilkhewl  252

         +g+lPfd++nl++llek+++g +++p+ + ++++sLlr +++vd+++R+t+e+i+kh w+

Q: 182   VGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWY  241

         **********************************************************95

Domain Sequence
(FASTA)

GLVKLGIHCV TCQKVAIKIV NREKLSESVL MKVEREIAIL KLIEHPHVLK LHDVYENKKY 60

LYLVLEHVSG GELFDYLVKK GRLTPKEARK FFRQIISALD FCHSHSICHR DLKPENLLLD 120

ERNNIRIADF GMASLQVGDS LLETSCGSPH YACPEVIRGE KYDGRKADVW SCGVILFALL 180

VGALPFDDDN LRQLLEKVKR GVFHMPHFIP PDCQSLLRGM IEVDAARRLT LEHIQKHIWY 240

Keyword

Alternative splicing; Apoptosis; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Exocytosis; Kinase; Magnesium; Metal-binding; Mitosis; Neurogenesis; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Cavia porcellus"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Gallus gallus"; ?>Ictidomys tridecemlineatus"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Nomascus leucogenys"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0050321	; F:tau-protein kinase activity
GO:0007409	; P:axonogenesis
GO:0030010	; P:establishment of cell polarity
GO:0031572	; P:G2/M transition DNA damage checkpoint
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0009411	; P:response to UV

KEGG

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; FALSE_NEG.

Prints

Created Date

20-Feb-2013