EPS-RAN-00029

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-RAN-00029

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	191.7	2.5E-56	230	483	254

Status

Reviewed

Ensembl Protein

ENSRNOP00000003859

UniProt Accession

Q80Z30;

Protein Name

Protein phosphatase 1E

Protein Synonyms/Alias

Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA;

Gene Name

Ppm1e

Gene Synonyms/Alias

Ppm1e; Camkn;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000024730	ENSRNOP00000003859	ENSRNOT00000003859

Organism

Rattus norvegicus

Functional Description

Protein phosphatase that inactivates multifunctional CaMkinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases.

Protein Length

750

Protein Sequence
(FASTA)

MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA EAAEASGEDP 60

GEDAATVEAA EEGVQDQDPE PEEEAVEEEA AAEGEEEEEE EEAAAPGHSA VPPPQPQLPP 120

LPPLPRPLSE RITREEVEGE SLDLCLQQLY KYNCPSFLAA ALARATSDEV LQSDLSAHCI 180

PKETDGTEGT VEIETVKLAR SVFSKLHEIC CNWVKDFPLR RRPQIYYETS IHAIKNMRRK 240

MEDKHVCIPD FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA 300

EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM LVRKGQAVEL 360

MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI GDAEHKPYIC GDADSASTVL 420

DGTEDYLILA CDGFYDTVNP DEAVKVVSDH LKENNGDSSM VAHKLVASAR DAGSSDNITV 480

IVVFLRDMNK AVNVSEESDW TENSFQGGQE DGGDDKENHG ECKRPWPQHQ CSAPADLGYE 540

GRVDSFTDRT SLSPGPQINV LEDPDYLDLT QIETSKPHST QFLPPVEMIG PGAPKKAYVN 600

ELIMEESSVT PSQPERSGAG ELLVSFNLGS TGQQICRMEN LSPVYSGLEN EQFKSPGKRA 660

SRLYHLRHHY SKRQRGFRFN PKFYSFFSAQ ESSRKIGISL SSLTRSGKRN KMLRSSLPWR 720

ENSWEGYSGN MAIRKRNNIS CPDLPWDYKI 750

Nucleotide Sequence
(FASTA)

ATGGCGGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT GTTCCTGGGC 60

GAGTTCCGCG GACCGTGCGG CGGCGGCGAG CCAGAGCCCG AACCCGAATC CGAGCCCGAG 120

CCCGAGCCCG AAGCGGAGCT GGTCGCGGCT GAGGCGGCCG AGGCTTCGGG GGAGGACCCG 180

GGGGAGGACG CTGCGACGGT AGAGGCGGCC GAGGAGGGGG TGCAGGACCA AGACCCGGAG 240

CCCGAAGAGG AGGCGGTGGA AGAGGAGGCG GCGGCCGAGG GCGAGGAGGA GGAGGAAGAG 300

GAGGAAGCGG CTGCCCCGGG GCACTCGGCC GTGCCGCCGC CGCAGCCCCA GCTGCCGCCG 360

CTGCCCCCGC TCCCGCGGCC GCTGTCGGAG CGCATCACCC GAGAGGAGGT GGAGGGCGAG 420

AGCCTGGACC TGTGCCTGCA GCAGCTCTAC AAATATAACT GCCCTTCCTT TCTGGCTGCC 480

GCTTTAGCCA GAGCCACATC AGATGAAGTC CTCCAGAGTG ACCTTTCTGC TCACTGTATC 540

CCAAAGGAGA CGGACGGCAC AGAAGGGACT GTGGAAATTG AGACAGTGAA ATTAGCCCGT 600

TCTGTCTTCA GCAAACTACA CGAGATTTGC TGCAATTGGG TGAAGGACTT CCCTCTACGC 660

AGGAGGCCAC AGATTTATTA TGAGACGTCA ATCCATGCCA TCAAGAACAT GCGAAGGAAA 720

ATGGAGGACA AACATGTCTG CATCCCTGAC TTTAACATGC TCTTCAACCT AGAGGACCAG 780

GAGGAACAAG CATACTTTGC AGTGTTTGAT GGCCATGGGG GAGTGGATGC TGCTATATAT 840

GCCTCGGTTC ACCTACACGT TAACTTAGTG CGCCAGGAGA TGTTTCCCCA TGACCCTGCT 900

GAGGCTCTCT GCCGGGCCTT CCGTGTCACA GATGAGCGGT TTGTGCAGAA GGCAGCCAGG 960

GAGAGTTTAC GATGTGGGAC CACAGGAGTA GTGACTTTTA TCAGAGGCAA CATGCTCCAT 1020

GTGGCATGGG TGGGCGACTC CCAGGTCATG CTGGTAAGAA AGGGCCAAGC TGTTGAACTC 1080

ATGAAGCCTC ACAAGCCAGA CAGAGAGGAT GAAAAGCAGC GAATTGAGGC CCTTGGAGGT 1140

TGTGTGGTTT GGTTTGGTGC CTGGAGGGTG AATGGAAGCC TGTCCGTCTC CAGAGCCATC 1200

GGAGATGCTG AACACAAGCC ATATATCTGT GGAGATGCAG ATTCTGCCTC CACTGTTCTG 1260

GATGGGACTG AAGACTACCT CATTCTGGCC TGTGATGGCT TCTATGATAC CGTGAACCCT 1320

GATGAGGCAG TGAAGGTTGT GTCTGACCAC TTGAAAGAGA ATAATGGAGA CAGCAGCATG 1380

GTTGCCCACA AATTAGTAGC ATCAGCTCGT GATGCGGGGT CAAGTGATAA CATCACTGTT 1440

ATTGTGGTAT TCCTGAGGGA CATGAACAAA GCTGTAAATG TTAGTGAGGA ATCAGATTGG 1500

ACAGAGAACT CTTTTCAAGG AGGGCAAGAA GATGGTGGGG ATGATAAGGA GAATCATGGA 1560

GAGTGCAAAC GCCCTTGGCC ACAGCACCAG TGCTCAGCAC CAGCCGATCT AGGCTATGAG 1620

GGGCGTGTGG ATTCATTCAC TGATAGAACT AGCCTGAGCC CAGGGCCCCA AATCAACGTG 1680

CTGGAAGATC CAGACTACTT AGATCTCACA CAAATAGAAA CAAGCAAACC TCACAGTACC 1740

CAATTTTTGC CACCAGTTGA GATGATTGGT CCTGGTGCAC CAAAGAAAGC ATATGTTAAT 1800

GAGCTAATAA TGGAGGAGAG CTCAGTCACG CCATCACAGC CTGAACGGAG TGGTGCTGGA 1860

GAGCTTCTGG TTTCTTTTAA TTTGGGTTCA ACAGGGCAAC AGATATGCAG AATGGAGAAC 1920

TTGTCCCCTG TCTATTCTGG GTTGGAAAAT GAACAGTTCA AATCCCCGGG GAAAAGAGCG 1980

TCTAGATTGT ATCATTTACG CCACCACTAC TCCAAAAGGC AGCGTGGATT CAGGTTTAAT 2040

CCAAAGTTTT ATTCATTTTT TTCTGCTCAA GAGTCTTCTC GAAAAATAGG CATTAGCCTC 2100

TCCTCACTTA CTCGAAGTGG GAAGAGAAAC AAGATGTTAA GAAGTTCTTT GCCATGGAGG 2160

GAAAATAGTT GGGAAGGATA TAGTGGAAAC ATGGCGATCA GAAAGCGTAA TAATATTTCA 2220

TGCCCAGACC TTCCCTGGGA CTATAAAATT TAA 2253

Domain Profile

S: 4     gvvsvngrrnemedavtsspnflkspekkdr..laffgvyDGhggsevakfcseklheil  61

         ++ +++++r++med++   p+f    + +d+   a+f+v+DGhgg ++a ++s +lh  l

Q: 230   SIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQeeQAYFAVFDGHGGVDAAIYASVHLHVNL  289

         567899**************99766555544459************************99

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdsklyvan  121

          ++         +  +al +aF  tde++  ++++  ++ ++G+t vv  i+++ l+va

Q: 290   VRQEM----FPHDPAEALCRAFRVTDERF--VQKAARESLRCGTTGVVTFIRGNMLHVAW  343

         99955....6788888999**********..7777788888*******************

S: 122   vGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdga.RvlGvLavsRalGd  180

         vGdS+++L r+g+avel + Hkp+redE++Rie+ gg v+ + ga Rv+G+L vsRa+Gd

Q: 344   VGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVV-WFGAwRVNGSLSVSRAIGD  402

         *************************************999.55899**************

S: 181   lylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle.kkeaeqeaa  239

          + kpy+  + +  s+ l+  +++liLa+DG +d+v++ eav++v d+l+ ++ + + +a

Q: 403   AEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKeNNGDSSMVA  462

         ************************************************9966778899**

S: 240   kkLvklalargskdnvsaivv  260

         +kLv+ a++ gs+dn+++ivv

Q: 463   HKLVASARDAGSSDNITVIVV  483

         *****************9997

Domain Sequence
(FASTA)

SIHAIKNMRR KMEDKHVCIP DFNMLFNLED QEEQAYFAVF DGHGGVDAAI YASVHLHVNL 60

VRQEMFPHDP AEALCRAFRV TDERFVQKAA RESLRCGTTG VVTFIRGNML HVAWVGDSQV 120

MLVRKGQAVE LMKPHKPDRE DEKQRIEALG GCVVWFGAWR VNGSLSVSRA IGDAEHKPYI 180

CGDADSASTV LDGTEDYLIL ACDGFYDTVN PDEAVKVVSD HLKENNGDSS MVAHKLVASA 240

RDAGSSDNIT VIVV 254

Keyword

Complete proteome; Cytoplasm; Hydrolase; Magnesium; Manganese; Metal-binding; Nucleus; Protein phosphatase; Reference proteome; Repeat.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005730	; C:nucleolus
GO:0005634	; C:nucleus
GO:0043234	; C:protein complex
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0035690	; P:cellular response to drug
GO:0006469	; P:negative regulation of protein kinase activity
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0051496	; P:positive regulation of stress fiber assembly

KEGG

rno:360593

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013