EKS-RAN-00514

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00514

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/TIF1	N/A	N/A	N/A	N/A	N/A

Status

Reviewed

Ensembl Protein

ENSRNOP00000031061

UniProt Accession

O08629; B2RYN5;

Protein Name

Transcription intermediary factor 1-beta

Protein Synonyms/Alias

TIF1-beta; E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; Nuclear corepressor KAP-1; Tripartite motif-containing protein 28;

Gene Name

Trim28

Gene Synonyms/Alias

Trim28; Kap1, Tif1b;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000027487	ENSRNOP00000031061	ENSRNOT00000029996

Organism

Rattus norvegicus

Functional Description

Nuclear corepressor for KRAB domain-containing zincfinger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6 (By similarity).

Protein Length

835

Protein Sequence
(FASTA)

MAASAAAATA AASAATAASA ASGSPGSGEG SAGGEKRPAA SSAAAASASA SSPAGGGGEA 60

QELLEHCGVC RERLRPERDP RLLPCLHSAC SACLGPATPA AANNSGDGGS AGDGAMVDCP 120

VCKQQCYSKD IVENYFMRDS GSKASSDSQD ANQCCTSCED NAPATSYCVE CSEPLCETCV 180

EAHQRVKYTK DHTVRSTGPA KTRDGERTVY CNVHKHEPLV LFCESCDTLT CRDCQLNAHK 240

DHQYQFLEDA VRNQRKLLAS LVKRLGDKHA TLQKNTKEVR SSIRQVSDVQ KRVQVDVKMA 300

ILQIMKELNK RGRVLVNDAQ KVTEGQQERL ERQHWTMTKI QKHQEHILRF ASWALESDNN 360

TALLLSKKLI YFQLHRALKM IVDPVEPHGE MKFQWDLNAW TKSAEAFGKI VAERPGTNST 420

GPGPMAPPRA PGPLSKQGSG SSQPMEVQEG YGFGTDDPYS SAEPHVSGMK RSRSGEGEVS 480

GLMRKVPRVS LERLDLDLTS DSQPPVFKVF PGSTTEDYNL IVIERGAAAA AAGQAGTVPP 540

GAPGAPPLPG MAIVKEEETE AAIGAPPAAP EGPETKPVLM ALTEGPGAEG PRLASPSGST 600

SSGLEVVAPE VTSAPVSGPG ILDDSATICR VCQKPGDLVM CNQCEFCFHL DCHLPSLQDV 660

PGEEWSCSLC HVLPDLKEED GSLSLDGADS TGVVAKLSPA NQRKCERVLL ALFCHEPCRP 720

LHQLATDSTF SMEQPGGTLD LTLIRARLQE KLSPPYSSPQ EFAQDVGRMF KQFNKLTEDK 780

ADVQSIIGLQ RFFETRMNDA FGDTKFSAVL VEPPPLNLPS AGLSSQELSG PGDGP 835

Nucleotide Sequence
(FASTA)

ATGGCGGCCT CGGCGGCGGC GGCGACTGCG GCGGCCTCGG CCGCGACGGC CGCGTCAGCG 60

GCCTCTGGTA GCCCAGGGTC GGGCGAAGGC TCGGCGGGCG GTGAGAAGCG CCCCGCTGCC 120

TCTTCAGCCG CGGCGGCCTC GGCCTCCGCG TCGTCCCCTG CGGGGGGCGG TGGCGAGGCG 180

CAGGAGCTTT TGGAGCACTG CGGCGTGTGT CGCGAGCGCT TGCGGCCTGA GCGGGATCCT 240

CGGCTGCTGC CCTGTCTGCA TTCGGCCTGT AGTGCCTGCC TTGGCCCCGC TACACCCGCC 300

GCAGCTAATA ATTCGGGGGA TGGCGGCTCG GCGGGCGACG GCGCTATGGT GGATTGTCCA 360

GTGTGCAAAC AACAGTGCTA CTCCAAAGAC ATCGTGGAGA ATTACTTTAT GCGTGATAGT 420

GGCAGTAAGG CCTCTTCTGA TTCCCAGGAT GCTAACCAGT GCTGCACTAG CTGTGAAGAT 480

AACGCCCCAG CCACTAGCTA TTGTGTGGAG TGCTCTGAAC CACTGTGTGA GACCTGTGTG 540

GAGGCTCACC AGCGGGTGAA ATACACCAAG GATCACACTG TGCGCTCCAC GGGTCCTGCT 600

AAGACTCGAG ATGGAGAACG AACAGTCTAT TGTAATGTGC ACAAGCATGA ACCCCTTGTG 660

CTGTTTTGTG AGAGCTGTGA CACGCTCACT TGCCGGGACT GCCAACTCAA TGCTCACAAG 720

GACCACCAGT ACCAATTCTT GGAAGATGCA GTAAGGAACC AACGTAAACT CTTGGCTTCA 780

CTGGTGAAAC GTCTTGGGGA CAAACATGCC ACACTGCAGA AAAACACCAA GGAGGTTCGA 840

AGCTCGATCC GCCAGGTGTC TGATGTGCAG AAGCGAGTGC AGGTTGATGT GAAGATGGCC 900

ATTCTGCAGA TCATGAAGGA GCTGAATAAA CGGGGACGAG TTCTGGTCAA TGATGCCCAG 960

AAGGTGACTG AGGGTCAGCA GGAACGTCTG GAGCGTCAGC ACTGGACCAT GACCAAAATC 1020

CAGAAGCACC AGGAACACAT TTTGCGCTTT GCCTCTTGGG CTTTGGAGAG TGATAACAAT 1080

ACAGCCCTCT TGCTCTCTAA GAAGCTGATT TATTTCCAGC TGCATCGGGC CCTCAAAATG 1140

ATTGTGGATC CTGTGGAGCC TCATGGTGAG ATGAAGTTTC AGTGGGATCT CAATGCCTGG 1200

ACCAAGAGTG CTGAAGCCTT TGGTAAGATT GTGGCTGAGC GTCCTGGTAC AAACTCCACG 1260

GGTCCTGGGC CCATGGCTCC TCCAAGAGCC CCAGGCCCCC TAAGCAAGCA AGGTTCTGGT 1320

AGTAGCCAGC CCATGGAAGT ACAAGAGGGC TATGGCTTTG GGACAGATGA TCCCTACTCA 1380

AGTGCAGAGC CACATGTATC AGGCATGAAG CGGTCCCGCT CTGGTGAGGG AGAAGTAAGT 1440

GGCCTCATGA GGAAGGTGCC ACGTGTGAGC CTTGAACGCC TGGATCTGGA TCTCACCTCT 1500

GATAGCCAGC CACCAGTCTT CAAGGTCTTT CCTGGAAGCA CCACTGAGGA CTACAATCTA 1560

ATTGTTATTG AGCGTGGTGC TGCTGCAGCA GCCGCTGGCC AGGCTGGGAC TGTGCCACCA 1620

GGAGCCCCTG GTGCCCCACC CCTGCCTGGC ATGGCCATTG TCAAGGAAGA AGAGACAGAA 1680

GCTGCTATTG GAGCCCCTCC TGCTGCCCCT GAGGGGCCTG AAACCAAGCC TGTGTTGATG 1740

GCTCTGACTG AAGGCCCTGG GGCTGAGGGA CCTCGTCTAG CTTCACCTAG TGGCAGTACC 1800

AGCTCAGGCT TGGAGGTGGT AGCTCCTGAG GTTACCTCAG CCCCAGTAAG TGGGCCAGGT 1860

ATCCTGGATG ACAGTGCCAC TATCTGCCGT GTATGCCAGA AGCCAGGTGA CCTGGTCATG 1920

TGCAACCAGT GCGAGTTTTG CTTCCACCTG GATTGTCACC TCCCTTCCCT GCAGGATGTT 1980

CCAGGGGAGG AATGGAGTTG CTCACTCTGC CACGTGCTCC CTGATCTAAA GGAGGAAGAT 2040

GGAAGCCTTA GCTTGGATGG AGCAGATAGC ACTGGTGTGG TAGCTAAACT CTCACCAGCC 2100

AACCAGCGGA AATGTGAGCG TGTTCTGCTG GCCCTTTTCT GCCATGAACC GTGCCGCCCC 2160

TTGCATCAGC TGGCTACCGA CTCTACATTC TCCATGGAGC AGCCTGGTGG TACCCTAGAC 2220

CTGACCTTGA TTCGTGCTCG CCTCCAAGAG AAGCTGTCAC CTCCTTATAG CTCCCCCCAG 2280

GAGTTTGCCC AGGATGTGGG CCGCATGTTC AAACAGTTCA ATAAGCTGAC TGAGGACAAG 2340

GCAGATGTTC AGTCCATCAT CGGCCTCCAG CGCTTCTTTG AGACACGAAT GAATGATGCC 2400

TTTGGTGACA CCAAGTTTTC TGCTGTGCTG GTAGAACCAC CACCACTGAA CCTTCCCAGT 2460

GCTGGCCTAA GTTCTCAGGA GCTGTCTGGT CCTGGTGATG GCCCCTGA 2508

Domain Profile

N/A

Domain Sequence
(FASTA)

N/A

Keyword

Acetylation; Bromodomain; Complete proteome; Isopeptide bond; Ligase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0016874	; F:ligase activity
GO:0008270	; F:zinc ion binding
GO:0001837	; P:epithelial to mesenchymal transition
GO:0045893	; P:positive regulation of transcription, DNA-dependent
GO:0016925	; P:protein sumoylation
GO:0006351	; P:transcription, DNA-dependent

KEGG

rno:116698

;

InterPros

IPR003649	; Bbox_C.
IPR001487	; Bromodomain.
IPR019786	; Zinc_finger_PHD-type_CS.
IPR000315	; Znf_B-box.
IPR011011	; Znf_FYVE_PHD.
IPR001965	; Znf_PHD.
IPR019787	; Znf_PHD-finger.
IPR001841	; Znf_RING.
IPR013083	; Znf_RING/FYVE/PHD.

Pfam

PF00628	; PHD; 1.
PF00643	; zf-B_box; 2.

SMARTs

SM00502	; BBC; 1.
SM00336	; BBOX; 2.
SM00297	; BROMO; 1.
SM00249	; PHD; 1.
SM00184	; RING; 2.

Prosites

PS00633	; BROMODOMAIN_1; FALSE_NEG.
PS50014	; BROMODOMAIN_2; FALSE_NEG.
PS50119	; ZF_BBOX; 2.
PS01359	; ZF_PHD_1; 1.
PS50016	; ZF_PHD_2; 1.
PS00518	; ZF_RING_1; FALSE_NEG.
PS50089	; ZF_RING_2; 1.

Prints

Created Date

20-Feb-2013