EKS-RAN-00381

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-RAN-00381

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	444.5	1.3E-133	275	524	250

Status

Reviewed

Ensembl Protein

ENSRNOP00000053093

UniProt Accession

F1LM93;

Protein Name

Tyrosine-protein kinase Yes

Protein Synonyms/Alias

p61-Yes;

Gene Name

Yes1

Gene Synonyms/Alias

Yes1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000037227	ENSRNOP00000053094	ENSRNOT00000056251
ENSRNOG00000037227	ENSRNOP00000053093	ENSRNOT00000056250

Organism

Rattus norvegicus

Functional Description

Non-receptor protein tyrosine kinase that is involved inthe regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).

Protein Length

541

Protein Sequence
(FASTA)

MGCIKSKENK SPAIKYTPEN PTEPVNTSAG HYGVEHATAA TTSSTKGASA NFNSLSMTPF 60

GGSSGVTPFG GASSSFSVVP SSYPTSLTGG VTIFVALYDY EARTTEDLSF KKGERFQIIN 120

NTEGDWWEAR SIATGKNGYI PSNYVAPADS IQAEEWYFGK MGRKDAERLL LNPGNQRGIF 180

LVRESETTKG AYSLSIRDWD EVRGDNVKHY KIRKLDNGGY YITTRAQFDT LQKLVKHYTE 240

HADGLCHKLT TVCPTVKPQT QGLAKDAWEI PRESLRLEVK LGQGCFGEVW MGTWNGTTKV 300

AIKTLKPGTM MPEAFLQEAQ IMKKLRHDKL VPLYAVVSEE PIYIVTEFMS KGILLDFLKE 360

GDGKYLKLPQ LVDMAAQIAD GMAYIERMNY IHRDLRAANI LVGENLVCKI ADFGLARLIE 420

DNEYTARQGA KFPIKWTAPE AALYGRFTIK SDVWSFGILQ TELVTKGRVP YPGMVNREVL 480

EQVERGYRMP CPQGCPESLH ELMNLCWKKD PDERPTFEYI QSFLEDYFTA TEPQYQPGEN 540

L 541

Nucleotide Sequence
(FASTA)

ATGGGCTGCA TTAAAAGTAA AGAAAACAAA AGTCCAGCCA TTAAATATAC ACCGGAAAAT 60

CCTACAGAGC CTGTAAACAC AAGTGCCGGT CATTATGGAG TGGAACATGC TACAGCTGCC 120

ACGACCTCTT CCACAAAGGG AGCATCAGCT AATTTTAACA GTCTTTCCAT GACACCCTTT 180

GGAGGGTCCT CTGGGGTGAC TCCTTTTGGA GGAGCATCTT CCTCATTCTC AGTGGTGCCA 240

AGTTCATATC CTACAAGTTT AACAGGTGGT GTCACTATAT TTGTGGCCTT ATATGATTAT 300

GAAGCTAGAA CTACAGAAGA CCTTTCCTTT AAGAAGGGTG AAAGATTTCA AATAATTAAC 360

AATACGGAAG GAGACTGGTG GGAAGCAAGA TCAATTGCTA CCGGAAAGAA TGGTTATATC 420

CCTAGCAATT ATGTAGCGCC TGCAGATTCC ATTCAGGCAG AAGAATGGTA TTTTGGCAAA 480

ATGGGGAGAA AAGATGCTGA AAGATTACTT CTGAATCCTG GGAATCAGCG AGGTATATTC 540

TTAGTAAGAG AAAGTGAAAC TACTAAAGGT GCTTATTCCC TCTCAATTCG TGATTGGGAT 600

GAGGTAAGGG GTGACAATGT GAAGCATTAC AAAATCAGAA AACTTGACAA CGGTGGATAC 660

TACATCACGA CCAGAGCTCA GTTTGATACT CTACAGAAAC TGGTGAAGCA TTACACAGAA 720

CATGCTGATG GATTATGCCA CAAATTAACA ACCGTCTGTC CAACTGTGAA ACCGCAGACT 780

CAAGGTCTCG CAAAAGACGC TTGGGAAATC CCTCGAGAGT CTTTGCGACT CGAGGTTAAA 840

CTAGGTCAAG GATGCTTTGG TGAAGTGTGG ATGGGAACAT GGAATGGAAC CACAAAAGTA 900

GCAATCAAAA CACTAAAGCC AGGTACAATG ATGCCAGAAG CATTCCTTCA AGAAGCTCAG 960

ATAATGAAAA AATTAAGACA CGATAAACTT GTTCCGCTCT ATGCAGTTGT TTCTGAAGAG 1020

CCAATTTATA TTGTCACTGA GTTTATGTCA AAAGGTATCT TGTTAGATTT CCTTAAAGAA 1080

GGAGATGGAA AGTATTTGAA GCTTCCACAG CTGGTTGATA TGGCTGCTCA GATTGCTGAT 1140

GGCATGGCAT ATATTGAAAG AATGAACTAT ATTCACCGAG ATCTCCGAGC TGCTAATATT 1200

CTTGTGGGAG AAAATCTTGT ATGCAAAATA GCAGATTTTG GCTTAGCAAG ACTAATTGAA 1260

GACAATGAAT ACACAGCAAG ACAAGGTGCA AAATTTCCAA TCAAGTGGAC AGCTCCTGAG 1320

GCTGCGCTTT ATGGTCGATT TACGATAAAG TCAGATGTGT GGTCATTTGG AATTCTACAG 1380

ACAGAGCTGG TAACAAAAGG AAGAGTGCCG TATCCAGGTA TGGTAAACCG AGAAGTGTTG 1440

GAACAAGTAG AGCGAGGGTA CAGAATGCCC TGCCCTCAGG GCTGCCCAGA ATCCCTCCAT 1500

GAACTGATGA ATCTTTGCTG GAAGAAGGAT CCTGATGAGA GACCAACATT TGAATATATT 1560

CAGTCATTCT TAGAAGACTA CTTCACTGCT ACAGAGCCGC AGTACCAACC AGGAGAAAAT 1620

TTATAA 1626

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         l+l+ klG+G+fGevw+g+w+g++kva+ktlk+gt++peafl+eaqi+kklrh+klv ly

Q: 275   LRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLY  334

         68999*******************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avvse ePiyivte+m+kG lldflke +gk+lklp+lvd+aaq+a+Gmay+e++n+ihr

Q: 335   AVVSE-EPIYIVTEFMSKGILLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHR  393

         ****9.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+a+n+lvge+lv+k+adfGlarlied+eyta++gak+PikWtaPea+ +g+ftiksdv

Q: 394   DLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV  453

         ************************************************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee  240

         WsfGil te+vtkG+vPypGm n+evleqvergyr+p+p+ cpe+l+el++ cwkkdp+e

Q: 454   WSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDE  513

         ************************************************************

S: 241   rPtfetlqevl  251

         rPtfe++q++l

Q: 514   RPTFEYIQSFL  524

         *******9986

Domain Sequence
(FASTA)

LRLEVKLGQG CFGEVWMGTW NGTTKVAIKT LKPGTMMPEA FLQEAQIMKK LRHDKLVPLY 60

AVVSEEPIYI VTEFMSKGIL LDFLKEGDGK YLKLPQLVDM AAQIADGMAY IERMNYIHRD 120

LRAANILVGE NLVCKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 180

SFGILQTELV TKGRVPYPGM VNREVLEQVE RGYRMPCPQG CPESLHELMN LCWKKDPDER 240

PTFEYIQSFL 250

Keyword

ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005794	; C:Golgi apparatus
GO:0005815	; C:microtubule organizing center
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0015758	; P:glucose transport
GO:0046777	; P:protein autophosphorylation

KEGG

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013