EPS-RAN-00031

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-RAN-00031

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	189.6	1.1E-55	153	407	255

Status

Reviewed

Ensembl Protein

ENSRNOP00000002530

UniProt Accession

Q9WVR7;

Protein Name

Protein phosphatase 1F

Protein Synonyms/Alias

Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2;

Gene Name

Ppm1f

Gene Synonyms/Alias

Ppm1f; Popx2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSRNOG00000037909	ENSRNOP00000002530	ENSRNOT00000002530

Organism

Rattus norvegicus

Functional Description

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Protein Length

450

Protein Sequence
(FASTA)

MASGAPQNSS QMACDGEIPG FLDTLLQDFP APLSLESPLP WKVPGTVLGQ EEVEAELTEL 60

AMGFLGSRNA PPAVAAAVTH EAISQLLQTD LSEFKRLPEQ EEEEEEEEER VLTTLLDAKG 120

LSRSFFNCLW EVCSQWQKRV PLTAQAPQRK WLVSIHAIRN TRRKMEDRHV SLPAFNHLFG 180

LSDSVHRAYF AVFDGHGGVD AARYASVHVH TNASHQPELL TDPAAALKEA FRHTDQMFLQ 240

KAKRERLQSG TTGVCALITG AALHVAWLGD SQVILVQQGQ VVKLMEPHKP ERQDEKSRIE 300

ALGGFVSLMD CWRVNGTLAV SRAIGDVFQK PYVSGEADAA SRELTGLEDY LLLACDGFFD 360

VVPHHEIPGL VHGHLLRQKG SGMHVAEELV AVARDRGSHD NITVMVVFLR DPLELLEGGG 420

QGAGGAQADV GSQDLSTGLS ELEINTSQRS 450

Nucleotide Sequence
(FASTA)

ATGGCCTCTG GAGCCCCACA GAACAGCAGC CAGATGGCCT GTGACGGCGA GATCCCAGGT 60

TTCCTGGACA CACTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 120

TGGAAGGTCC CAGGGACAGT CCTGGGCCAG GAGGAGGTGG AGGCTGAGCT GACGGAGCTG 180

GCAATGGGTT TCCTGGGCAG CAGGAATGCA CCCCCAGCAG TTGCTGCGGC TGTGACCCAC 240

GAGGCAATTT CCCAGCTGCT TCAGACAGAC CTCTCTGAAT TTAAGAGGTT GCCTGAACAA 300

GAGGAAGAGG AGGAGGAGGA GGAAGAGAGA GTCCTCACGA CCCTACTGGA TGCCAAAGGC 360

CTGTCCCGGA GCTTCTTTAA CTGTCTTTGG GAAGTATGCA GCCAGTGGCA GAAACGGGTG 420

CCACTGACTG CCCAGGCCCC TCAGCGCAAG TGGCTGGTCT CTATCCATGC CATCCGGAAC 480

ACACGACGAA AGATGGAGGA TCGACACGTG TCCCTTCCTG CTTTCAACCA CCTCTTCGGC 540

TTGTCTGACT CCGTGCACCG CGCCTACTTC GCTGTGTTTG ATGGTCATGG AGGTGTGGAT 600

GCAGCACGGT ATGCCTCTGT CCACGTGCAC ACCAACGCCT CTCACCAGCC AGAGCTGCTC 660

ACAGACCCTG CAGCAGCTCT CAAAGAAGCC TTCCGGCATA CTGACCAGAT GTTTCTCCAG 720

AAAGCCAAGC GAGAGCGACT GCAGAGCGGT ACCACAGGTG TGTGTGCGCT CATCACAGGA 780

GCAGCCCTGC ATGTTGCCTG GCTTGGAGAC TCCCAGGTCA TCCTGGTACA GCAAGGACAG 840

GTAGTGAAGC TGATGGAGCC ACACAAACCG GAACGACAGG ATGAGAAATC ACGCATTGAA 900

GCGCTGGGCG GCTTTGTTTC TCTCATGGAC TGCTGGAGAG TCAACGGGAC CCTGGCAGTG 960

TCCAGAGCCA TTGGGGATGT CTTCCAGAAG CCTTATGTGT CTGGCGAGGC AGATGCAGCA 1020

TCCAGAGAGC TGACAGGCTT GGAGGACTAC CTGCTGCTTG CCTGTGACGG TTTCTTCGAT 1080

GTCGTCCCCC ACCATGAAAT CCCGGGTCTT GTCCATGGCC ACTTGCTCAG GCAGAAGGGC 1140

AGTGGGATGC ACGTTGCTGA GGAGCTGGTG GCTGTAGCCC GTGACCGGGG TTCCCATGAC 1200

AACATTACAG TCATGGTGGT TTTCCTTAGG GACCCCCTGG AGCTGCTGGA GGGTGGGGGG 1260

CAGGGGGCAG GGGGTGCCCA GGCAGATGTA GGAAGTCAGG ACTTGTCCAC TGGCCTCTCA 1320

GAACTTGAGA TCAATACCTC ACAGAGAAGC TAA 1353

Domain Profile

S: 3     vgvvsvngrrnemedavtsspnflkspekkdr..laffgvyDGhggsevakfcseklhei  60

         v++ ++ + r++med++ s p f    + +d    a+f+v+DGhgg ++a+++s ++h+

Q: 153   VSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSvhRAYFAVFDGHGGVDAARYASVHVHTN  212

         6678999**************999987777775568************************

S: 61    laeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdsklyva  120

            + e      ++  +al++aF +td+ +  l++++ +  +sG+t v+ali++  l+va

Q: 213   ASHQPE----LLTDPAAALKEAFRHTDQMF--LQKAKRERLQSGTTGVCALITGAALHVA  266

         ****77....66777889************..9999999999******************

S: 121   nvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLavsRalGd  180

          +GdS+++L ++g++v+l + Hkp+r+dE++Rie+ gg v l d+ Rv+G LavsRa+Gd

Q: 267   WLGDSQVILVQQGQVVKLMEPHKPERQDEKSRIEALGGFVSLMDCWRVNGTLAVSRAIGD  326

         ********************************************99**************

S: 181   lylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle.kkeaeqeaa  239

         ++ kpyv+ e +  s +lt  +++l La+DG +dvv+  e+  lv+ +l  +k +   +a

Q: 327   VFQKPYVSGEADAASRELTGLEDYLLLACDGFFDVVPHHEIPGLVHGHLLrQKGSGMHVA  386

         *********************************************97766488999****

S: 240   kkLvklalargskdnvsaivv  260

         ++Lv++a++rgs+dn++++vv

Q: 387   EELVAVARDRGSHDNITVMVV  407

         *****************9997

Domain Sequence
(FASTA)

VSIHAIRNTR RKMEDRHVSL PAFNHLFGLS DSVHRAYFAV FDGHGGVDAA RYASVHVHTN 60

ASHQPELLTD PAAALKEAFR HTDQMFLQKA KRERLQSGTT GVCALITGAA LHVAWLGDSQ 120

VILVQQGQVV KLMEPHKPER QDEKSRIEAL GGFVSLMDCW RVNGTLAVSR AIGDVFQKPY 180

VSGEADAASR ELTGLEDYLL LACDGFFDVV PHHEIPGLVH GHLLRQKGSG MHVAEELVAV 240

ARDRGSHDNI TVMVV 255

Keyword

Apoptosis; Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0048471	; C:perinuclear region of cytoplasm
GO:0043234	; C:protein complex
GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0046872	; F:metal ion binding
GO:0035690	; P:cellular response to drug
GO:0097193	; P:intrinsic apoptotic signaling pathway
GO:0033137	; P:negative regulation of peptidyl-serine phosphorylation
GO:0044387	; P:negative regulation of protein kinase activity by regulation of protein phosphorylation
GO:0045892	; P:negative regulation of transcription, DNA-dependent
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0050921	; P:positive regulation of chemotaxis
GO:0043280	; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0010634	; P:positive regulation of epithelial cell migration
GO:0051894	; P:positive regulation of focal adhesion assembly
GO:0010628	; P:positive regulation of gene expression
GO:0045927	; P:positive regulation of growth
GO:0051496	; P:positive regulation of stress fiber assembly

KEGG

rno:287931

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013