EKS-SUS-00322

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SUS-00322

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	449.9	2.7E-135	271	519	249

Status

Reviewed

Ensembl Protein

ENSSSCP00000004769

UniProt Accession

A1Y2K1;

Protein Name

Tyrosine-protein kinase Fyn

Protein Synonyms/Alias

Proto-oncogene c-Fyn; p59-Fyn;

Gene Name

FYN

Gene Synonyms/Alias

FYN;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSSSCG00000004421	ENSSSCP00000028580	ENSSSCT00000034071
ENSSSCG00000004421	ENSSSCP00000004771	ENSSSCT00000004886
ENSSSCG00000004421	ENSSSCP00000029295	ENSSSCT00000035136
ENSSSCG00000004421	ENSSSCP00000004770	ENSSSCT00000004885
ENSSSCG00000004421	ENSSSCP00000004769	ENSSSCT00000004884
ENSSSCG00000004421	ENSSSCP00000029911	ENSSSCT00000036277
ENSSSCG00000004421	ENSSSCP00000028728	ENSSSCT00000036530
ENSSSCG00000004421	ENSSSCP00000029844	ENSSSCT00000034742

Organism

Sus scrofa

Functional Description

Non-receptor tyrosine-protein kinase that plays a rolein many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta- catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1 (By similarity).

Protein Length

537

Protein Sequence
(FASTA)

MGCVQCKDKE ATKLTEERDG SLNQSSGFRY GTDPTPQHYP SFGVTSIPNY NNFHAAGGQG 60

LTVFGGVSSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD LSFHKGEKFQ ILNSSEGDWW 120

EARSLTTGET GYIPSNYVAP VDSIQAEEWY FGKLGRKDAE RQLLSFGNPR GTFLIRESET 180

TKGAYSLSIR DWDDMKGDHV KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC 240

RLVVPCHKGM PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT 300

LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL LDFLKDGEGR 360

ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN GLICKIADFG LARLIEDNEY 420

TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV TKGRVPYPGM NNREVLEQVE 480

RGYRMPCPQD CPISLHELMI HCWKKDPEER PTFEYLQGFL EDYFTATEPQ YQPGENL 537

Nucleotide Sequence
(FASTA)

ATGGGCTGTG TGCAATGTAA GGATAAAGAA GCAACAAAAC TGACGGAGGA GCGGGATGGC 60

AGCCTGAACC AGAGCTCCGG CTTCCGCTAT GGCACAGACC CCACTCCTCA GCACTACCCC 120

AGCTTCGGCG TGACCTCCAT CCCAAACTAC AACAACTTCC ATGCAGCCGG GGGCCAAGGA 180

CTCACCGTCT TCGGGGGCGT GAGCTCTTCG TCTCACACTG GGACCTTGCG TACGAGAGGA 240

GGAACAGGAG TGACGCTGTT TGTGGCCCTT TACGATTATG AAGCCCGGAC GGAAGATGAC 300

CTGAGTTTTC ACAAAGGAGA AAAATTTCAA ATACTGAACA GCTCGGAAGG AGACTGGTGG 360

GAGGCCCGCT CCTTGACAAC AGGAGAGACG GGTTACATTC CCAGCAATTA CGTGGCTCCA 420

GTTGACTCCA TCCAGGCAGA AGAGTGGTAC TTTGGAAAAC TCGGCCGAAA AGATGCTGAG 480

CGACAGCTTT TGTCCTTTGG AAACCCAAGA GGTACCTTTC TTATCCGTGA GAGTGAAACC 540

ACCAAAGGTG CCTATTCGCT TTCCATCCGT GACTGGGATG ATATGAAAGG AGACCATGTC 600

AAACATTATA AAATTCGCAA ACTTGACAAC GGTGGGTATT ATATCACCAC CCGGGCCCAG 660

TTTGAAACAC TTCAGCAGCT TGTACAGCAT TACTCAGAGA GAGCCGCAGG TCTCTGCTGC 720

CGCCTAGTCG TTCCCTGTCA CAAAGGGATG CCAAGGCTTA CCGATCTGTC TGTCAAAACC 780

AAAGATGTCT GGGAAATCCC TCGAGAATCC CTGCAGTTGA TCAAGAGACT GGGCAATGGG 840

CAGTTTGGGG AAGTATGGAT GGGTACCTGG AACGGAAACA CAAAAGTAGC CATAAAGACT 900

CTCAAACCAG GCACAATGTC CCCAGAATCA TTCCTCGAGG AGGCACAGAT CATGAAGAAG 960

TTGAAGCATG ACAAACTGGT CCAGCTCTAT GCCGTGGTAT CAGAGGAACC CATCTACATC 1020

GTCACCGAGT ACATGAATAA AGGAAGCTTA CTCGATTTCT TAAAGGATGG AGAAGGAAGA 1080

GCCCTGAAGT TACCAAACCT CGTGGACATG GCAGCACAGG TTGCTGCAGG AATGGCGTAC 1140

ATTGAGCGCA TGAATTATAT CCACAGAGAC CTTCGGTCGG CAAACATTCT CGTGGGGAAT 1200

GGGCTCATTT GCAAGATCGC CGACTTTGGA TTAGCCAGGT TGATCGAGGA CAACGAGTAC 1260

ACAGCAAGAC AAGGTGCAAA GTTCCCCATT AAGTGGACAG CCCCCGAAGC AGCCCTGTAC 1320

GGGAGGTTCA CAATCAAGTC TGATGTGTGG TCTTTTGGAA TCTTACTCAC AGAGCTGGTC 1380

ACCAAAGGAA GAGTTCCGTA CCCAGGCATG AACAACCGGG AGGTGCTGGA GCAGGTGGAG 1440

CGCGGCTACA GGATGCCCTG TCCGCAGGAC TGCCCCATCT CTCTCCACGA GCTCATGATC 1500

CACTGCTGGA AAAAGGACCC CGAAGAGAGG CCCACTTTCG AGTACTTGCA GGGCTTCCTG 1560

GAAGACTACT TCACCGCGAC AGAGCCCCAG TATCAACCCG GTGAAAACCT GTAA 1614

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         l+l+k+lG+GqfGevw+g+w+g++kva+ktlk+gt+spe+fleeaqi+kkl+h+klv+ly

Q: 271   LQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLY  330

         689*********************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avvse ePiyivte+m+kGslldflk+ eg++lklp+lvd+aaqva+Gmay+e++n+ihr

Q: 331   AVVSE-EPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHR  389

         ****9.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+++n+lvg+ l++k+adfGlarlied+eyta++gak+PikWtaPea+ +g+ftiksdv

Q: 390   DLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV  449

         ************************************************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee  240

         WsfGillte+vtkG+vPypGm+n+evleqvergyr+p+p++cp +l+el+++cwkkdpee

Q: 450   WSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEE  509

         ************************************************************

S: 241   rPtfetlqev  250

         rPtfe+lq +

Q: 510   RPTFEYLQGF  519

         *******976

Domain Sequence
(FASTA)

LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT LKPGTMSPES FLEEAQIMKK LKHDKLVQLY 60

AVVSEEPIYI VTEYMNKGSL LDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD 120

LRSANILVGN GLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 180

SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMI HCWKKDPEER 240

PTFEYLQGF 249

Keyword

Adaptive immunity; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Developmental protein; Immunity; Kinase; Lipoprotein; Manganese; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Palmitate; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005768	; C:endosome
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0050798	; P:activated T cell proliferation
GO:0048813	; P:dendrite morphogenesis
GO:0050966	; P:detection of mechanical stimulus involved in sensory perception of pain
GO:0030900	; P:forebrain development
GO:0010629	; P:negative regulation of gene expression
GO:0042177	; P:negative regulation of protein catabolic process
GO:0001764	; P:neuron migration
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0046777	; P:protein autophosphorylation
GO:0008360	; P:regulation of cell shape
GO:0045471	; P:response to ethanol
GO:0050852	; P:T cell receptor signaling pathway

KEGG

ssc:791125

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013