EKS-XET-00336

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-XET-00336

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	416.9	3.4E-125	46	298	253

Status

Reviewed

Ensembl Protein

ENSXETP00000022754

UniProt Accession

P62205;

Protein Name

Serine/threonine-protein kinase PLK1

Protein Synonyms/Alias

Plx1; Polo-like kinase 1; PLK-1;

Gene Name

plk1

Gene Synonyms/Alias

plk1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSXETG00000010344	ENSXETP00000022754	ENSXETT00000022754

Organism

Xenopus tropicalis

Functional Description

Plays multiple essential roles during mitosis.Phosphorylates the N-terminal domain of cdc25, which leads to cyclin b-cdc2 activation and mitotic entry. Also required for organization of bipolar spindles, and for exit from mitosis. Phosphorylates tpx2 (By similarity).

Protein Length

600

Protein Sequence
(FASTA)

RTMAQVAGKK LTVAPEAGKP PGIPGSSSSV KEIPEILVDP RTRRRYLRGR FLGKGGFAKC 60

YEITDLESRE VFAGKIVPKT MLLKPHQKDK MTMEIAIQRS LDHRHVVGFH GFFEDNDFVY 120

VVLELCRRRS LLELHKRRKA VTEPEARYYL KQTILGCQYL HSNRVIHRDL KLGNLFLNDE 180

MEVKIGDFGL ATKVEYDGER KKTLCGTPNY IAPEVLGKKG HSFEVDIWSI GCIMYTLLVG 240

KPPFETSCLK ETYMRIKKNE YSVPKHINPM ATALIQKMLR SEPTSRPTID DLLNDEFFTT 300

GYIPSRLPTT CLTVPPRFSI APSTIDQSLR KPLTAINKGQ DSPLVEKQSV PAKEEEMQQP 360

ESTEPADCYL SEMLQQLTCV NAVKPSERAV IRQEEAEDPA SVPIFWVSKW VDYSDKYGLG 420

YQLCDNSVGV LFNDSTRLIM YNDGDSLQYI ERNNSESYLN VRSYPAALTK KITLLKYFRN 480

YMSEHLLKAG ANITPREGDE LARLPFLRTW FRTRSAIILH LSNGTVQINF FQDHTKIILC 540

PLMAAVSYID EKREFHTYKL SLIQEFGCCK ELASRLRYAR TMVEKLQSSK SGVAHVKASA 600

Nucleotide Sequence
(FASTA)

CGCACTATGG CTCAAGTAGC CGGTAAGAAA CTGACTGTGG CCCCAGAGGC CGGTAAACCC 60

CCGGGGATCC CCGGTTCCTC GTCATCCGTC AAAGAGATTC CAGAGATTCT GGTGGATCCC 120

CGCACCCGGA GGCGATACCT GAGGGGGAGA TTTCTGGGCA AAGGTGGGTT CGCTAAATGC 180

TACGAGATCA CCGACCTGGA GAGCCGGGAG GTATTTGCTG GGAAGATTGT GCCCAAGACC 240

ATGCTGCTCA AGCCCCACCA GAAGGACAAA ATGACCATGG AGATCGCCAT CCAGCGCAGT 300

CTTGACCACC GGCATGTCGT GGGCTTCCAT GGCTTCTTTG AGGACAATGA CTTCGTGTAT 360

GTGGTACTTG AGCTGTGCCG ACGGAGGTCT CTATTGGAGC TGCACAAGAG GAGGAAAGCT 420

GTAACAGAGC CAGAAGCTCG CTACTATTTG AAACAGACCA TTTTGGGGTG CCAGTATCTC 480

CATAGCAACA GAGTCATTCA CAGAGACCTC AAGCTTGGCA ACTTGTTCCT TAATGATGAA 540

ATGGAGGTCA AAATAGGTGA CTTTGGGCTG GCAACCAAAG TGGAATATGA TGGCGAGCGC 600

AAGAAAACCC TCTGTGGCAC TCCAAACTAC ATTGCACCTG AAGTGTTGGG CAAGAAGGGC 660

CACAGTTTTG AAGTGGACAT ATGGTCAATA GGATGCATCA TGTACACGCT GCTGGTGGGA 720

AAACCCCCCT TTGAAACATC ATGCCTAAAA GAAACCTACA TGAGGATTAA AAAGAATGAA 780

TACTCTGTCC CCAAGCACAT TAACCCCATG GCGACAGCAC TTATACAGAA GATGCTTCGT 840

TCCGAACCAA CCTCAAGGCC CACGATAGAT GACTTGCTAA ATGATGAGTT CTTTACTACT 900

GGCTACATTC CTTCCCGGCT CCCCACAACC TGCTTAACTG TGCCCCCAAG GTTTTCCATT 960

GCACCCAGCA CTATTGATCA AAGCTTAAGG AAGCCACTTA CTGCAATCAA TAAAGGACAA 1020

GACTCTCCAC TGGTGGAGAA GCAGTCGGTT CCTGCAAAGG AAGAAGAAAT GCAGCAGCCG 1080

GAGTCCACAG AGCCTGCAGA CTGTTACCTA TCTGAGATGC TTCAGCAGCT GACTTGTGTG 1140

AATGCAGTCA AGCCTTCTGA GAGAGCGGTT ATCCGCCAAG AGGAAGCAGA GGATCCAGCA 1200

TCCGTCCCCA TATTCTGGGT CAGCAAATGG GTGGACTACT CTGATAAATA TGGATTAGGA 1260

TACCAGCTGT GTGATAACAG TGTTGGGGTG CTCTTTAATG ATTCAACGCG GTTGATAATG 1320

TACAATGACG GAGACAGCCT GCAGTACATT GAGAGGAATA ATTCAGAGTC TTACCTCAAC 1380

GTGCGCTCCT ACCCTGCTGC TTTAACCAAA AAGATCACAT TGCTCAAGTA CTTCAGAAAT 1440

TACATGAGTG AGCACCTACT GAAGGCCGGT GCAAACATAA CACCCCGGGA GGGGGATGAA 1500

CTGGCTCGTC TACCCTTCTT ACGAACCTGG TTCAGGACAC GCAGCGCCAT CATCCTCCAC 1560

CTGAGCAATG GGACTGTTCA GATAAACTTC TTCCAGGATC ACACAAAGAT AATCCTGTGC 1620

CCCCTTATGG CTGCTGTGTC CTACATTGAT GAAAAGCGTG AGTTCCATAC GTACAAGCTG 1680

AGCCTCATTC AAGAATTTGG CTGCTGCAAG GAGCTCGCCA GCCGTCTCCG CTACGCACGC 1740

ACAATGGTGG AGAAACTACA GAGCTCCAAG TCCGGTGTTG CACATGTGAA GGCCTCTGCA 1800

TAG 1803

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y rg++lGkGgfakcye++dle++evfa k+vpk++l k++q++k+++ei+i rsl+h++

Q: 46    YLRGRFLGKGGFAKCYEITDLESREVFAGKIVPKTMLLKPHQKDKMTMEIAIQRSLDHRH  105

         889*********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         +v ++ ffed+++vy+vlelc+rrsl el+krrka+tepe+ryyl+q + g++ylh++r+

Q: 106   VVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEPEARYYLKQTILGCQYLHSNRV  165

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         +hrdlklgnlfln+e+evkigdfGlat++e+++erkktlcGtPnyiaPevl kkghs+ev

Q: 166   IHRDLKLGNLFLNDEMEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLGKKGHSFEV  225

         ************************************************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         diWs+Gci+ytllvGkPPfet++lkety +ikk+eys+P+++++ a+ li+k+l++eP++

Q: 226   DIWSIGCIMYTLLVGKPPFETSCLKETYMRIKKNEYSVPKHINPMATALIQKMLRSEPTS  285

         ************************************************************

S: 241   rpsleevlkdefl  253

         rp+++++l+def+

Q: 286   RPTIDDLLNDEFF  298

         ************8

Domain Sequence
(FASTA)

YLRGRFLGKG GFAKCYEITD LESREVFAGK IVPKTMLLKP HQKDKMTMEI AIQRSLDHRH 60

VVGFHGFFED NDFVYVVLEL CRRRSLLELH KRRKAVTEPE ARYYLKQTIL GCQYLHSNRV 120

IHRDLKLGNL FLNDEMEVKI GDFGLATKVE YDGERKKTLC GTPNYIAPEV LGKKGHSFEV 180

DIWSIGCIMY TLLVGKPPFE TSCLKETYMR IKKNEYSVPK HINPMATALI QKMLRSEPTS 240

RPTIDDLLND EFF 253

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0000776	; C:kinetochore
GO:0030496	; C:midbody
GO:0005634	; C:nucleus
GO:0005819	; C:spindle
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0031572	; P:G2/M transition DNA damage checkpoint
GO:0007067	; P:mitosis
GO:0071168	; P:protein localization to chromatin

KEGG

xtr:407937

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013