EKS-CAE-00132

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00132

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	422.1	1.4E-126	253	522	270

Status

Reviewed

Ensembl Protein

F09E5.1.1

UniProt Accession

Q19266; A7VJC8;

Protein Name

Protein kinase C-like 3

Protein Synonyms/Alias

Atypical protein kinase C-3; aPKC3;

Gene Name

pkc-3

Gene Synonyms/Alias

pkc-3; aPKC; F09E5.1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
F09E5.1	F09E5.1.2	F09E5.1.2
F09E5.1	F09E5.1.1	F09E5.1.1

Organism

Caenorhabditis elegans

Functional Description

Required for the normal progression of embryogenesis andviability of the organism. Plays an indispensable role in establishing embryonic polarity and in recruiting and maintaining par-6 to the periphery, through interaction with par-3. Required for epithelial cell polarity in the distal spermatheca. Phosphorylates serine residues of num-1.

Protein Length

597

Protein Sequence
(FASTA)

MSSPTSLEED GDIKLKTRFQ GQVVVLYARP PLILDDFFAL LKDACKQHKK QDITVKWIDE 60

DGDPISIDSQ MELDEAVRCL NSSQEAELNI HVFVGKPELP GLPCQGEDKT VYRRGARRWK 120

KIYLYNGHRF QAKRLNRRIQ CFICHDYIWG IGRQGFRCVD CRLCVHKKCH RHVRTHCGQA 180

LQGPNIIPMA PASGSLKGAR SNTSSSTTRS GGGIDNGAFH EHEIESPGST SHDASRAMNG 240

NGSSKWAVSL NDFRLLTVIG RGSYAKVVQA EHVSTRQIYA IKIIKKEMFN EDEDIDWVQT 300

EKSVFEAASN YPFLVGLHSC FQTESRLFFV IEFVPGGDLM FHMQQQRKLP EEHARFYSGE 360

IILALHFLHS RGIIYRDLKL DNVLIDAEGH IKLTDYGMCK ENIKDGDLTS TFCGTPNYIA 420

PEILRGDEYG FSVDWWALGV LMFEMMAGRS PFDIVGMQNS EENTEDYLFQ IILERQIRIP 480

RSLSVRASGI LKGFLNKDPT ERLGCKLDIN EGLRDMKEHQ FFRGFIDWEA LEQKAVAPPY 540

HPAVESDRDL THFDHQFTDE LPQLSPDNPD VIARIDQSEF DGFEYVNPLQ MSREDSV 597

Nucleotide Sequence
(FASTA)

ATGTCGTCTC CGACATCATT AGAGGAGGAC GGAGATATTA AGCTAAAAAC GAGGTTTCAA 60

GGACAGGTAG TAGTACTCTA TGCTCGACCA CCGCTCATTC TCGATGACTT TTTTGCACTT 120

TTGAAAGATG CTTGTAAACA ACACAAAAAA CAAGATATTA CTGTAAAATG GATTGATGAA 180

GATGGAGATC CAATCTCAAT AGACTCACAA ATGGAACTTG ATGAAGCAGT TCGATGTTTG 240

AATTCTAGTC AAGAAGCCGA GTTGAACATT CACGTTTTTG TTGGAAAACC AGAGCTGCCC 300

GGGCTTCCGT GTCAAGGAGA AGACAAAACT GTATATCGAA GAGGTGCTCG ACGATGGAAG 360

AAAATTTATC TGTACAATGG GCATCGATTC CAAGCGAAAC GGCTGAATCG CCGTATTCAA 420

TGCTTTATTT GTCATGACTA CATATGGGGA ATTGGACGGC AAGGCTTTCG ATGTGTGGAT 480

TGTCGTTTAT GCGTGCACAA AAAATGCCAT CGGCATGTGC GAACGCATTG TGGTCAGGCT 540

CTCCAAGGCC CGAATATTAT CCCAATGGCT CCTGCAAGTG GTTCTCTAAA GGGTGCTCGA 600

TCGAATACAA GCTCATCTAC AACGCGGTCG GGTGGAGGAA TTGATAACGG AGCATTCCAC 660

GAGCACGAAA TCGAATCACC CGGATCCACG AGTCATGATG CTAGCAGAGC TATGAATGGA 720

AATGGGTCGT CAAAATGGGC AGTTTCTCTG AACGATTTCC GTCTTTTAAC AGTTATTGGA 780

CGTGGATCTT ATGCAAAAGT TGTGCAAGCA GAACATGTTT CAACTCGTCA AATTTACGCG 840

ATAAAAATTA TCAAAAAGGA GATGTTCAAT GAAGATGAAG ATATCGATTG GGTACAGACG 900

GAGAAGTCAG TATTCGAAGC AGCGTCAAAT TATCCATTCC TTGTTGGATT ACATTCTTGC 960

TTCCAGACCG AATCCCGCCT GTTCTTTGTC ATCGAATTCG TTCCTGGAGG TGATCTGATG 1020

TTCCACATGC AACAGCAACG GAAGCTTCCA GAAGAGCACG CTCGATTCTA TTCGGGTGAA 1080

ATAATTCTTG CTCTTCATTT CCTTCATTCT CGCGGAATCA TTTATCGTGA TCTCAAATTG 1140

GACAATGTTC TGATTGACGC TGAAGGACAC ATAAAACTGA CAGATTATGG AATGTGTAAA 1200

GAGAATATTA AGGATGGAGA TTTAACTTCA ACTTTCTGTG GAACACCCAA TTATATTGCA 1260

CCAGAAATAT TGAGAGGTGA TGAATATGGA TTCAGTGTTG ATTGGTGGGC ATTGGGAGTG 1320

TTAATGTTTG AAATGATGGC TGGTCGATCT CCATTCGATA TTGTTGGAAT GCAGAATTCC 1380

GAGGAAAATA CAGAAGATTA TCTTTTCCAA ATCATTCTTG AACGACAGAT TCGAATCCCT 1440

AGATCACTTT CTGTACGGGC TTCTGGTATT CTAAAGGGAT TCCTGAACAA GGATCCAACA 1500

GAACGTCTGG GATGTAAGCT TGACATTAAT GAAGGTCTTC GTGATATGAA AGAACATCAA 1560

TTCTTCCGTG GATTCATTGA TTGGGAAGCT TTGGAACAGA AGGCTGTCGC ACCTCCATAT 1620

CATCCAGCTG TGGAAAGTGA TCGCGATTTG ACACACTTTG ATCATCAGTT CACCGACGAA 1680

CTTCCACAAT TATCACCTGA CAATCCAGAT GTTATTGCAC GAATTGATCA ATCAGAATTT 1740

GATGGATTTG AATACGTGAA TCCTCTACAA ATGAGTCGGG AAGATTCAGT CTGA 1794

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp  60

         f+ll+v+G+Gs++kv+ ae+ +t+++yaik++kk+++++d+d++++++ek v++ as+ p

Q: 253   FRLLTVIGRGSYAKVVQAEHVSTRQIYAIKIIKKEMFNEDEDIDWVQTEKSVFEAASNYP  312

         899*********************************************************

S: 61    flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg  120

         flv l+scfqte+rlffv+e+v GGdlmfh+q++rkl+ee+arfy+ eiilal+flh++g

Q: 313   FLVGLHSCFQTESRLFFVIEFVPGGDLMFHMQQQRKLPEEHARFYSGEIILALHFLHSRG  372

         ************************************************************

S: 121   iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks  180

         iiyrdlkldnvl+daeGhikl+d+G+cke+i +g+ tstfcGtP+yiaPeil+++eyg+s

Q: 373   IIYRDLKLDNVLIDAEGHIKLTDYGMCKENIKDGDLTSTFCGTPNYIAPEILRGDEYGFS  432

         ************************************************************

S: 181   vdwwalGvllyemlagqsPfe........gededelfesilekevlyPkslskeaveilk  232

         vdwwalGvl++em+ag+sPf+        +++ed+lf+ ile+++++P+sls++a+ ilk

Q: 433   VDWWALGVLMFEMMAGRSPFDivgmqnseENTEDYLFQIILERQIRIPRSLSVRASGILK  492

         ***************************9999*****************************

S: 233   glltkdpekrlGvk...eegeedikehaff  259

         g+l+kdp++rlG+k   +eg +d+keh+ff

Q: 493   GFLNKDPTERLGCKldiNEGLRDMKEHQFF  522

         *************544699**********9

Domain Sequence
(FASTA)

FRLLTVIGRG SYAKVVQAEH VSTRQIYAIK IIKKEMFNED EDIDWVQTEK SVFEAASNYP 60

FLVGLHSCFQ TESRLFFVIE FVPGGDLMFH MQQQRKLPEE HARFYSGEII LALHFLHSRG 120

IIYRDLKLDN VLIDAEGHIK LTDYGMCKEN IKDGDLTSTF CGTPNYIAPE ILRGDEYGFS 180

VDWWALGVLM FEMMAGRSPF DIVGMQNSEE NTEDYLFQII LERQIRIPRS LSVRASGILK 240

GFLNKDPTER LGCKLDINEG LRDMKEHQFF 270

Keyword

ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Fertilization; Gastrulation; Gonadal differentiation; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016324	; C:apical plasma membrane
GO:0030864	; C:cortical actin cytoskeleton
GO:0005829	; C:cytosol
GO:0005524	; F:ATP binding
GO:0004697	; F:protein kinase C activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0008270	; F:zinc ion binding
GO:0008105	; P:asymmetric protein localization
GO:0010171	; P:body morphogenesis
GO:0030154	; P:cell differentiation
GO:0009792	; P:embryo development ending in birth or egg hatching
GO:0007163	; P:establishment or maintenance of cell polarity
GO:0007369	; P:gastrulation
GO:0008406	; P:gonad development
GO:0007506	; P:gonadal mesoderm development
GO:0040035	; P:hermaphrodite genitalia development
GO:0035556	; P:intracellular signal transduction
GO:0040027	; P:negative regulation of vulval development
GO:0006898	; P:receptor-mediated endocytosis
GO:0007338	; P:single fertilization

KEGG

cel:CELE_F09E5.1

;

InterPros

IPR000961	; AGC-kinase_C.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR000270	; OPR_PB1.
IPR012233	; PKC_zeta.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF00564	; PB1; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 1.
SM00666	; PB1; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013