EKS-CAE-00216

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00216

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	343.2	1.2E-102	47	298	252

Status

Reviewed

Ensembl Protein

F15A2.6b

UniProt Accession

Q19469; A3FPL0; Q9BMN6;

Protein Name

Serine/threonine kinase SAD-1

Protein Synonyms/Alias

Synapses of Amphids Defective;

Gene Name

sad-1

Gene Synonyms/Alias

sad-1; F15A2.6;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
F15A2.6	F15A2.6a	F15A2.6a
F15A2.6	F15A2.6b	F15A2.6b

Organism

Caenorhabditis elegans

Functional Description

Regulates both neuronal polarity and synapticorganization when bound to strd-1. Kinase activity is required for the establishment, but not the maintenance, of both processes. Binding to nab-1 is essential for role in restricting axonal fate during neuronal polarization but is not required for regulating synapse morphology.

Protein Length

835

Protein Sequence
(FASTA)

MFEALKEVLG EINSKLAAVN NELSSKIMSE NIVSTRPVAQ AQYCGPYKLE KTLGKGQTGL 60

VKTGTHCITG RKVAIKIVNK EKLSESVLQK VEREIAIMKL IEHPHVLHLY DVYENKKYLY 120

LLLEHVSGGE LFDYLVRKGR LMSKEARKFF RQIISALDFC HAHNICHRDL KPENLLLDER 180

NNIKVADFGM ASLQVEGSML ETSCGSPHYA CPEVIRGEKY DGRKADVWSC GVILYALLVG 240

ALPFDDDNLR NLLEKVKRGV FHIPHFVPAD VQSLLRAMIE VDPGKRYSLA DVFKHPWVSG 300

TTKADPELEL PMSQVVQTHV IPGEDSIDPD VLRHMNCLGC FKDKQKLINE LLSPKHNTEK 360

MVYFLLLDRK RRRPAQEDDT EIVLRGAAQN NDPPKKRTDS SRTSRYPMGS IADGSPINPR 420

KTYGRNQKSG RHSSLGGSPT ESPRSSTRDL FGSSSSGSYS ARAGEDRDRG RSASRSTNSY 480

HYYTQPVDPQ TLAEAARHVR DAQERRESRD SGRGSSRKES KDRSDKSASS SSCKNDASST 540

SSVPHKYSPP SVMSESVVVS SSTMNSTNSS TNSLIAGNSQ TSIGSTSGPW RSKLNNIKNS 600

FLGTPRFHRR KMSNGTAESD SEDSQMIDTT DLVKKSWFGS LASSMSVERD DTHCVPVQGK 660

TLNSIKAELI RAFLQIHELS HSVVGQNCFR VEYKRGPTVG GSVFSRGIKM NVDIIPSPQQ 720

VVIAGETPTY VVQFVLLAGP VRRFKRLVEH LSAILQNSTQ QRADRQQQAA LMVRPRRLSD 780

SSVGSACSDS ESNASSINMI ARHSDKTETT SATSSDPYGP SPSMRSVGSG TGITS 835

Nucleotide Sequence
(FASTA)

ATGTTTGAAG CTCTCAAGGA AGTCCTCGGA GAGATAAATA GCAAACTGGC GGCCGTGAAT 60

AATGAGCTGA GCTCGAAAAT CATGTCGGAA AACATTGTAT CGACGCGTCC AGTGGCGCAG 120

GCTCAATATT GCGGTCCATA CAAACTTGAG AAGACGCTTG GAAAAGGTCA AACTGGGTTG 180

GTAAAAACGG GAACTCATTG CATTACGGGC CGTAAAGTGG CAATCAAGAT AGTCAACAAA 240

GAAAAACTCA GTGAATCTGT TCTACAAAAG GTCGAAAGAG AAATTGCAAT TATGAAGTTG 300

ATAGAGCATC CACATGTGCT TCATTTGTAC GATGTCTACG AAAATAAGAA ATATTTATAT 360

TTGCTACTAG AACATGTTAG CGGTGGCGAG CTGTTCGACT ACCTTGTGCG GAAAGGACGG 420

CTGATGTCGA AAGAAGCGCG AAAGTTTTTT CGTCAAATCA TCTCTGCGCT CGATTTTTGC 480

CATGCTCATA ACATTTGTCA TCGAGATTTA AAACCGGAAA ACTTATTACT AGACGAGAGG 540

AATAACATAA AAGTAGCAGA TTTTGGAATG GCTAGTCTGC AAGTGGAAGG GTCGATGTTG 600

GAGACGAGTT GTGGATCTCC ACATTATGCA TGTCCAGAAG TTATTCGGGG TGAAAAGTAC 660

GACGGTCGAA AAGCAGACGT TTGGAGTTGT GGCGTTATCC TCTACGCATT ATTAGTTGGT 720

GCGCTTCCAT TTGATGATGA CAATCTCCGA AATCTTCTCG AAAAAGTGAA ACGTGGAGTT 780

TTTCATATCC CACATTTCGT ACCTGCTGAT GTGCAGAGTT TACTGCGGGC AATGATAGAA 840

GTAGACCCGG GGAAACGATA TTCCCTAGCC GACGTGTTCA AACATCCATG GGTATCCGGA 900

ACAACAAAGG CAGATCCTGA ATTGGAGCTT CCAATGTCAC AAGTTGTGCA GACGCACGTA 960

ATACCTGGTG AAGATTCGAT TGATCCGGAT GTCTTGAGGC ATATGAACTG CTTAGGATGT 1020

TTCAAAGACA AACAGAAGTT GATTAATGAA TTATTGTCGC CAAAACACAA CACTGAAAAG 1080

ATGGTATACT TTCTCCTTCT TGATCGAAAA CGACGGCGCC CTGCACAGGA AGACGACACA 1140

GAAATCGTTC TGCGCGGTGC CGCCCAGAAC AACGATCCGC CTAAAAAACG GACGGATTCT 1200

TCGAGAACAT CAAGATATCC CATGGGAAGC ATTGCTGATG GAAGTCCGAT CAATCCACGA 1260

AAAACGTATG GAAGGAATCA AAAGTCTGGA CGGCATTCAA GTTTGGGAGG TTCTCCTACA 1320

GAGTCACCGA GAAGTTCAAC TAGAGATCTG TTCGGTTCAT CGTCTAGCGG ATCTTACTCT 1380

GCAAGGGCTG GAGAAGATCG GGATAGAGGG CGATCTGCTT CAAGGTCCAC CAACAGCTAT 1440

CATTACTACA CTCAACCAGT CGATCCACAG ACTCTTGCGG AAGCAGCACG TCACGTACGT 1500

GACGCCCAGG AACGGAGGGA GAGCAGAGAC AGCGGTAGAG GGTCGTCTAG AAAAGAAAGC 1560

AAGGACAGAA GTGACAAAAG TGCCAGTAGT AGTAGTTGCA AAAATGATGC CTCGTCAACG 1620

TCTTCGGTGC CCCATAAGTA CAGCCCACCA TCAGTCATGT CGGAGAGTGT GGTAGTGTCT 1680

AGTAGTACGA TGAATTCTAC GAACTCGTCA ACAAATAGTT TGATAGCTGG TAACAGTCAG 1740

ACAAGCATTG GGTCGACTAG CGGTCCATGG CGATCAAAAC TCAACAACAT CAAAAACTCA 1800

TTCTTGGGAA CTCCGAGATT CCATCGGCGG AAAATGTCAA ATGGGACGGC AGAAAGTGAC 1860

AGTGAAGACA GCCAGATGAT TGACACAACT GACCTTGTCA AAAAGTCCTG GTTCGGATCT 1920

CTTGCTTCCA GTATGAGCGT CGAACGCGAT GACACCCACT GTGTGCCTGT TCAAGGGAAA 1980

ACATTGAACA GCATCAAAGC GGAGCTCATA CGGGCATTCC TTCAAATTCA TGAACTTAGT 2040

CACTCAGTAG TTGGTCAAAA TTGCTTTAGA GTAGAATATA AACGAGGTCC GACAGTTGGT 2100

GGAAGCGTTT TCAGCCGTGG AATCAAAATG AATGTTGATA TTATCCCATC TCCTCAACAA 2160

GTAGTGATAG CTGGAGAAAC TCCGACATAT GTAGTACAGT TTGTTCTGCT GGCGGGACCA 2220

GTTCGGAGGT TTAAACGACT TGTGGAACAC TTGTCAGCAA TTCTGCAAAA TTCTACTCAA 2280

CAAAGAGCTG ATCGACAACA ACAAGCAGCC CTAATGGTTC GACCAAGGAG GTTAAGTGAC 2340

TCCAGCGTTG GTAGTGCTTG TTCGGACAGT GAAAGCAATG CGAGCAGTAT TAATATGATA 2400

GCGCGGCATT CGGATAAAAC TGAAACAACT TCTGCCACGT CATCTGACCC TTACGGTCCA 2460

TCCCCGTCAA TGCGATCTGT TGGAAGTGGA ACAGGAATTA CATCGTAA 2508

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkklseeslekikrEieilkklkhpni  60

         ykl ktlG+g++g Vk+ +h  t++kva+Ki++k+klse+ l+k++rEi+i+k ++hp++

Q: 47    YKLEKTLGKGQTGLVKTGTHCITGRKVAIKIVNKEKLSESVLQKVEREIAIMKLIEHPHV  106

         99**********************************************************

S: 61    ikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgiv  120

         ++l++v+e+kk+lyl+le++sggeLfdy+++kgrl +keark+f+q++sa++++h+++i+

Q: 107   LHLYDVYENKKYLYLLLEHVSGGELFDYLVRKGRLMSKEARKFFRQIISALDFCHAHNIC  166

         ************************************************************

S: 121   HrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkvD  180

         HrDlKpeNllld+++nik+aDFG+a+l  eg++let+cGsp+Ya+Pe+++g+kY+g+k+D

Q: 167   HRDLKPENLLLDERNNIKVADFGMASLQVEGSMLETSCGSPHYACPEVIRGEKYDGRKAD  226

         ************************************************************

S: 181   vWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpekR  240

         vWs+Gv+Lyal++g+lPfd++nl++llek+++g ++ip+ + ++++sLlr +++vdp kR

Q: 227   VWSCGVILYALLVGALPFDDDNLRNLLEKVKRGVFHIPHFVPADVQSLLRAMIEVDPGKR  286

         ************************************************************

S: 241   atieeilkhewl  252

         +++ ++ kh+w+

Q: 287   YSLADVFKHPWV  298

         ***********7

Domain Sequence
(FASTA)

YKLEKTLGKG QTGLVKTGTH CITGRKVAIK IVNKEKLSES VLQKVEREIA IMKLIEHPHV 60

LHLYDVYENK KYLYLLLEHV SGGELFDYLV RKGRLMSKEA RKFFRQIISA LDFCHAHNIC 120

HRDLKPENLL LDERNNIKVA DFGMASLQVE GSMLETSCGS PHYACPEVIR GEKYDGRKAD 180

VWSCGVILYA LLVGALPFDD DNLRNLLEKV KRGVFHIPHF VPADVQSLLR AMIEVDPGKR 240

YSLADVFKHP WV 252

Keyword

Alternative splicing; ATP-binding; Cell junction; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Synapse; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0030424	; C:axon
GO:0030054	; C:cell junction
GO:0030425	; C:dendrite
GO:0045202	; C:synapse
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0050321	; F:tau-protein kinase activity
GO:0008088	; P:axon cargo transport
GO:0007411	; P:axon guidance
GO:0034613	; P:cellular protein localization
GO:0007163	; P:establishment or maintenance of cell polarity
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0030516	; P:regulation of axon extension
GO:0007416	; P:synapse assembly
GO:0048489	; P:synaptic vesicle transport

KEGG

cel:CELE_F15A2.6

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013