EKS-CAE-00050

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00050

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	316.2	2.7E-94	26	348	323

Status

Reviewed

Ensembl Protein

F39H11.3

UniProt Accession

P90866;

Protein Name

Cyclin-dependent kinase 8

Protein Synonyms/Alias

Cell division protein kinase 8; Mediator complex subunit cdk-8; Mediator of RNA polymerase II transcription subunit cdk-8;

Gene Name

cdk-8

Gene Synonyms/Alias

cdk-8; F39H11.3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
F39H11.3	F39H11.3	F39H11.3

Organism

Caenorhabditis elegans

Functional Description

Component of the Mediator complex, a coactivatorinvolved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity).

Protein Length

588

Protein Sequence
(FASTA)

MTLMIDENFK KQLAQRRERV EDLFYFENSK EIGRGTYGLV YKAVPKKQNG QFPNKEYALK 60

MIEGQGFSMS ACREIALFRE LRHPNLICLQ RVFLTNEKKV WLLLDYAEHD LWHVIKHHRT 120

AKSKKVPIMV PRNMVKNILF QILSGMHYLH SNWVLHRDLK PANILLMGDG PPDMRGRVKI 180

ADLGFSRIYA NPLKPMAELD PVVVTFWYRA PELLLGAKHY TKAIDVWAIG CIFAELLTAE 240

PLFFCKEEDI KAQNPYHYDQ VKRIFHLLGY PSDADWPDMK KMPDHQRLLS DARNEGTPIQ 300

TFPNSLHRYF DKWKINSQSS PYRLLVKLLT VDPTKRVSCE EAMNDIYFRK MERPPRETDD 360

VFNKYPIPYA KKEQQMTVAP DQAQQQHQQQ QVQMQQQPQM GQQQMMGQPQ MVQPQMGQPP 420

MGGAHPGVVA PDGHPHQMMQ QQQHPQQHHM QYQGMHDPMQ GGMDEGPQAK MMRMGNVPVG 480

RYAPMPPPYG APQDYHPQQG PPMVQMMQQP GPSGYYPQRP GQPTGAVPGP GPQGYMNPQM 540

GMQMGMRAPG VPPQGYMPGR GMAPPQMGQQ QPGPNQQQQQ QWQQQYHR 588

Nucleotide Sequence
(FASTA)

ATGACATTAA TGATTGATGA AAACTTCAAA AAACAATTGG CACAGCGTCG AGAGAGAGTA 60

GAAGACCTTT TCTACTTTGA AAATTCAAAA GAAATCGGCA GAGGAACATA TGGATTAGTT 120

TATAAAGCAG TTCCGAAAAA GCAAAATGGA CAATTTCCTA ACAAAGAATA CGCATTAAAA 180

ATGATTGAGG GACAAGGATT TTCAATGTCT GCATGCAGAG AAATTGCTCT ATTCAGAGAA 240

TTAAGGCATC CGAATTTAAT CTGTCTTCAA CGTGTATTTC TTACCAACGA AAAAAAAGTC 300

TGGCTTTTAT TGGATTATGC TGAACACGAT CTCTGGCACG TTATCAAACA TCATCGAACA 360

GCTAAAAGCA AGAAAGTCCC AATTATGGTG CCACGGAATA TGGTCAAAAA TATATTGTTT 420

CAAATTTTAT CAGGAATGCA TTATTTACAT TCAAACTGGG TACTACATCG AGATCTCAAA 480

CCAGCAAATA TTCTATTAAT GGGTGATGGA CCACCTGACA TGAGAGGAAG GGTAAAAATT 540

GCTGATTTGG GATTTTCAAG AATCTATGCA AATCCTCTGA AACCGATGGC TGAATTAGAT 600

CCAGTAGTTG TCACATTCTG GTACAGAGCA CCTGAATTGC TTCTCGGAGC AAAGCATTAT 660

ACAAAGGCTA TTGACGTATG GGCTATCGGA TGCATTTTTG CCGAACTTCT TACCGCTGAA 720

CCATTATTTT TCTGTAAAGA GGAAGACATC AAGGCACAAA ATCCATATCA CTATGATCAA 780

GTGAAAAGAA TATTCCATCT ACTCGGATAC CCATCAGATG CTGATTGGCC TGATATGAAG 840

AAAATGCCGG ATCATCAAAG ACTTCTAAGC GATGCTAGAA ATGAAGGAAC ACCAATTCAA 900

ACATTTCCAA ACAGCTTGCA TCGATACTTT GATAAATGGA AAATCAATTC ACAAAGCTCT 960

CCATATAGAT TACTTGTCAA ATTGCTCACA GTTGACCCTA CAAAACGTGT CAGTTGTGAA 1020

GAAGCGATGA ATGATATTTA TTTTAGAAAA ATGGAAAGAC CACCAAGGGA GACTGACGAC 1080

GTGTTCAATA AATATCCAAT TCCATACGCG AAGAAGGAAC AGCAGATGAC GGTTGCACCA 1140

GATCAAGCAC AACAACAGCA TCAACAACAA CAAGTTCAAA TGCAACAACA ACCACAAATG 1200

GGACAGCAAC AAATGATGGG ACAACCTCAA ATGGTACAAC CTCAGATGGG ACAACCACCA 1260

ATGGGTGGAG CACATCCCGG AGTTGTTGCT CCAGATGGAC ATCCACATCA AATGATGCAG 1320

CAACAACAGC ATCCACAACA GCATCATATG CAATATCAAG GAATGCATGA TCCAATGCAA 1380

GGTGGAATGG ATGAAGGACC ACAAGCAAAA ATGATGAGAA TGGGAAATGT TCCTGTTGGA 1440

AGATATGCAC CGATGCCTCC TCCATACGGT GCTCCACAAG ACTATCACCC GCAACAAGGT 1500

CCACCAATGG TGCAAATGAT GCAACAACCG GGACCATCCG GCTACTATCC ACAACGACCA 1560

GGACAACCAA CAGGAGCAGT TCCTGGACCA GGACCTCAAG GATATATGAA TCCACAAATG 1620

GGAATGCAAA TGGGCATGCG TGCTCCAGGA GTTCCACCAC AAGGATATAT GCCTGGTCGT 1680

GGAATGGCTC CCCCTCAAAT GGGACAACAG CAACCCGGTC CAAATCAACA GCAACAACAG 1740

CAATGGCAAC AACAATATCA TCGATAA 1767

Domain Profile

S: 1     yeklekigeGtygkvykakdke.tge....vvAlKkirlekekegvpitalrEisllkel  55

         +e+ ++ig+Gtyg vyka  k+ +g+     +AlK+i    e +g++++a+rEi+l++el

Q: 26    FENSKEIGRGTYGLVYKAVPKKqNGQfpnkEYALKMI----EGQGFSMSACREIALFREL  81

         78899*************9865244444447999999....899****************

S: 56    khknivkLlevvakdkkklyLvfefleqdLkkllkkkkkk.......klsleevkslllq  108

         +h+n++ L+ v+ +++kk++L+++++e+dL++++k+++++       ++++++vk++l+q

Q: 82    RHPNLICLQRVFLTNEKKVWLLLDYAEHDLWHVIKHHRTAkskkvpiMVPRNMVKNILFQ  141

         *************999*********************9999999**99************

S: 109   lleglaylHsnkilHRDlKpqNlLiske......gklklaDfGlarafssplk...tytk  159

         +l+g++ylHsn++lHRDlKp+N+L++ +      g++k+aD+G++r +++plk   ++++

Q: 142   ILSGMHYLHSNWVLHRDLKPANILLMGDgppdmrGRVKIADLGFSRIYANPLKpmaELDP  201

         **************************99999999**************88887555799*

S: 160   evvTlwYraPelLlgakeYstavDiWsvgcilaelltrkplfqgkse.........idql  210

         +vvT+wYraPelLlgak+Y++a+D+W++gci+aellt +plf +k+e          dq+

Q: 202   VVVTFWYRAPELLLGAKHYTKAIDVWAIGCIFAELLTAEPLFFCKEEdikaqnpyhYDQV  261

         ************************************************************

S: 211   erifkllgtpsekvwpevsklpeykk.........sfpkkkkkklkekvkklk....eka  257

         +rif+llg ps+++wp+++k+p+++          +  ++ +++l+++++k+k    ++

Q: 262   KRIFHLLGYPSDADWPDMKKMPDHQRllsdarnegTPIQTFPNSLHRYFDKWKinsqSSP  321

         ************************943556655542222223355555555444321347

S: 258   ldllekllaldpkkRisakealqhkyf  284

         ++ll kll++dp+kR+s++ea+++ yf

Q: 322   YRLLVKLLTVDPTKRVSCEEAMNDIYF  348

         9************************98

Domain Sequence
(FASTA)

FENSKEIGRG TYGLVYKAVP KKQNGQFPNK EYALKMIEGQ GFSMSACREI ALFRELRHPN 60

LICLQRVFLT NEKKVWLLLD YAEHDLWHVI KHHRTAKSKK VPIMVPRNMV KNILFQILSG 120

MHYLHSNWVL HRDLKPANIL LMGDGPPDMR GRVKIADLGF SRIYANPLKP MAELDPVVVT 180

FWYRAPELLL GAKHYTKAID VWAIGCIFAE LLTAEPLFFC KEEDIKAQNP YHYDQVKRIF 240

HLLGYPSDAD WPDMKKMPDH QRLLSDARNE GTPIQTFPNS LHRYFDKWKI NSQSSPYRLL 300

VKLLTVDPTK RVSCEEAMND IYF 323

Keyword

Activator; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0010171	; P:body morphogenesis
GO:0009792	; P:embryo development ending in birth or egg hatching
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0040027	; P:negative regulation of vulval development
GO:0002119	; P:nematode larval development
GO:0040010	; P:positive regulation of growth rate
GO:0006468	; P:protein phosphorylation
GO:0006355	; P:regulation of transcription, DNA-dependent
GO:0000003	; P:reproduction
GO:0006351	; P:transcription, DNA-dependent

KEGG

cel:CELE_F39H11.3

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013