EKS-CAE-00128

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00128

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKG	477.2	1.7E-143	160	419	260

Status

Reviewed

Ensembl Protein

F55A8.2f.1

UniProt Accession

O76360; Q688A8; Q688A9; Q7KPJ2; Q8MXG6; Q8MXG7;

Protein Name

cGMP-dependent protein kinase egl-4

Protein Synonyms/Alias

Egg-laying defective protein 4;

Gene Name

egl-4

Gene Synonyms/Alias

egl-4; cgr-1, odr-9; F55A8.2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
F55A8.2	F55A8.2a.1	F55A8.2a.1
F55A8.2	F55A8.2f.2	F55A8.2f.2
F55A8.2	F55A8.2d	F55A8.2d
F55A8.2	F55A8.2f.1	F55A8.2f.1
F55A8.2	F55A8.2e.1	F55A8.2e.1
F55A8.2	F55A8.2a.2	F55A8.2a.2
F55A8.2	F55A8.2e.2	F55A8.2e.2
F55A8.2	F55A8.2b.1	F55A8.2b.1
F55A8.2	F55A8.2b.2	F55A8.2b.2
F55A8.2	F55A8.2c.2	F55A8.2c.2
F55A8.2	F55A8.2c.1	F55A8.2c.1

Organism

Caenorhabditis elegans

Functional Description

Promotes chemoreceptor gene expression in response toincreased cGMP levels by antagonizing the gene repression functions of the class II HDAC hda-4 and the mef-2 transcription factor. Represses body size and lifespan through the dbl-1 and insulin pathways, respectively. May also signal through daf-3 and/or daf-5. Role in egg-laying and dauer formation.

Protein Length

470

Protein Sequence
(FASTA)

MKKVYVVSIF QNLSEDRISK MADVMDQDYY DGGHYIIRQG EKGDAFFVIN SGQVKVTQQI 60

EGETEPREIR VLNQGDFFGE RALLGEEVRT ANIIAQAPGV EVLTLDRESF GKLIGDLESL 120

KKDYGDKERL AQVVREPPSP VKIVDDFREE FAQVTLKNVK RLATLGVGGF GRVELVCVNG 180

DKAKTFALKA LKKKHIVDTR QQEHIFAERN IMMETSTDWI VKLYKTFRDQ KFVYMLLEVC 240

LGGELWTTLR DRGHFDDYTA RFYVACVLEG LEYLHRKNIV YRDLKPENCL LANTGYLKLV 300

DFGFAKKLAS GRKTWTFCGT PEYVSPEIIL NKGHDQAADY WALGIYICEL MLGRPPFQAS 360

DPMKTYTLIL KGVDALEIPN RRIGKTATAL VKKLCRDNPG ERLGSGSGGV NDIRKHRWFM 420

GFDWEGLRSR TLKPPILPKV SNPADVTNFD NYPPDNDVPP DEFSGWDEGF 470

Nucleotide Sequence
(FASTA)

ATGAAGAAGG TTTATGTGGT ATCAATCTTC CAAAATTTGT CGGAAGATCG CATCTCAAAG 60

ATGGCCGACG TCATGGATCA GGACTATTAT GATGGTGGAC ATTATATCAT TCGACAGGGT 120

GAAAAGGGTG ACGCATTCTT CGTAATCAAC TCAGGACAAG TGAAAGTCAC CCAACAAATC 180

GAAGGAGAAA CCGAGCCCCG AGAAATCCGT GTGCTCAATC AAGGAGACTT CTTTGGAGAA 240

CGTGCACTGC TCGGCGAGGA GGTCCGTACA GCCAATATTA TTGCACAAGC TCCAGGAGTT 300

GAAGTACTTA CTTTGGATCG AGAATCATTC GGAAAGCTTA TCGGAGATCT TGAAAGCCTT 360

AAAAAAGACT ATGGAGATAA GGAACGTTTG GCTCAAGTTG TCCGAGAGCC GCCGAGCCCT 420

GTGAAGATTG TCGACGACTT CCGAGAGGAG TTTGCACAGG TTACACTGAA AAATGTGAAG 480

AGGCTAGCGA CACTTGGAGT TGGAGGATTT GGAAGAGTTG AACTTGTCTG TGTAAATGGA 540

GATAAGGCAA AGACGTTTGC GCTGAAGGCT CTGAAGAAGA AGCATATTGT TGATACCCGG 600

CAACAGGAGC ACATCTTTGC CGAGCGGAAT ATTATGATGG AAACTTCAAC TGATTGGATT 660

GTCAAGCTTT ATAAGACTTT TAGAGATCAA AAGTTTGTGT ACATGCTGCT CGAGGTATGC 720

CTTGGCGGTG AACTGTGGAC GACACTCCGC GATCGAGGAC ACTTTGACGA TTACACCGCC 780

CGTTTCTACG TGGCCTGCGT GCTCGAGGGT CTCGAATATC TGCATCGCAA GAATATTGTC 840

TACCGAGATT TGAAGCCCGA AAACTGTCTG CTCGCCAACA CTGGATATCT GAAACTTGTG 900

GATTTCGGAT TCGCAAAGAA ACTCGCGTCT GGAAGGAAGA CGTGGACATT CTGTGGAACA 960

CCGGAATACG TGTCACCTGA AATTATTCTC AATAAGGGAC ACGATCAAGC CGCCGACTAT 1020

TGGGCTCTTG GAATATATAT CTGTGAACTG ATGCTCGGAC GGCCACCGTT CCAGGCTAGT 1080

GATCCTATGA AGACGTATAC ATTGATCCTA AAGGGAGTAG ACGCACTGGA GATTCCGAAT 1140

CGTCGGATTG GAAAGACGGC GACGGCGTTG GTGAAGAAGC TCTGCAGGGA TAACCCGGGA 1200

GAACGGCTCG GCAGTGGAAG TGGTGGGGTG AATGATATTC GGAAGCATCG GTGGTTTATG 1260

GGATTCGATT GGGAAGGATT GAGAAGTAGG ACGTTGAAAC CTCCAATTCT GCCGAAGGTG 1320

TCCAACCCAG CAGACGTGAC GAATTTCGAC AATTATCCAC CAGATAATGA TGTTCCACCA 1380

GACGAGTTTT CCGGATGGGA TGAGGGATTC TAG 1413

Domain Profile

S: 2     kviatlgvggfgrvelvkvksdeskafalkilkkkhivdtkqqehvlsekrileeaksdf  61

         k++atlgvggfgrvelv+v+ d++k+falk+lkkkhivdt+qqeh+++e++i++e+++d+

Q: 160   KRLATLGVGGFGRVELVCVNGDKAKTFALKALKKKHIVDTRQQEHIFAERNIMMETSTDW  219

         899*********************************************************

S: 62    ivklyrtfkdekyvyllleaclggelwtilrdrgsfddktakfivacvvealeylhskni  121

         ivkly+tf+d+k+vy+lle+clggelwt+lrdrg+fdd ta+f+vacv+e+leylh+kni

Q: 220   IVKLYKTFRDQKFVYMLLEVCLGGELWTTLRDRGHFDDYTARFYVACVLEGLEYLHRKNI  279

         ************************************************************

S: 122   iyrdlkpenllldeegylklvdfgfakklksgkktwtfcgtpeyvapeiilnkghdlsvd  181

         +yrdlkpen+ll+++gylklvdfgfakkl+sg+ktwtfcgtpeyv+peiilnkghd+++d

Q: 280   VYRDLKPENCLLANTGYLKLVDFGFAKKLASGRKTWTFCGTPEYVSPEIILNKGHDQAAD  339

         ************************************************************

S: 182   ywalgilvyelltgkppfsgvdplktynlilkgidklelp.rkitkeaedlikklcrdnp  240

         ywalgi+++el+ g+ppf+++dp+kty+lilkg+d+le+p r+i+k+a++l+kklcrdnp

Q: 340   YWALGIYICELMLGRPPFQASDPMKTYTLILKGVDALEIPnRRIGKTATALVKKLCRDNP  399

         ****************************************899*****************

S: 241   aerlgylkggikdikkhkwf  260

         +erlg+ +gg++di+kh+wf

Q: 400   GERLGSGSGGVNDIRKHRWF  419

         *******************9

Domain Sequence
(FASTA)

KRLATLGVGG FGRVELVCVN GDKAKTFALK ALKKKHIVDT RQQEHIFAER NIMMETSTDW 60

IVKLYKTFRD QKFVYMLLEV CLGGELWTTL RDRGHFDDYT ARFYVACVLE GLEYLHRKNI 120

VYRDLKPENC LLANTGYLKL VDFGFAKKLA SGRKTWTFCG TPEYVSPEII LNKGHDQAAD 180

YWALGIYICE LMLGRPPFQA SDPMKTYTLI LKGVDALEIP NRRIGKTATA LVKKLCRDNP 240

GERLGSGSGG VNDIRKHRWF 260

Keyword

Alternative splicing; ATP-binding; cGMP; cGMP-binding; Coiled coil; Complete proteome; Developmental protein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0030553	; F:cGMP binding
GO:0004692	; F:cGMP-dependent protein kinase activity
GO:0046872	; F:metal ion binding
GO:0008340	; P:determination of adult lifespan
GO:0008286	; P:insulin receptor signaling pathway
GO:0030308	; P:negative regulation of cell growth
GO:0040015	; P:negative regulation of multicellular organism growth
GO:0046621	; P:negative regulation of organ growth
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0046662	; P:regulation of oviposition
GO:0030431	; P:sleep

KEGG

cel:CELE_F55A8.2

;

InterPros

IPR000961	; AGC-kinase_C.
IPR016232	; cGMP-dependent_protein_kinase.
IPR002374	; cGMP_dep_kinase.
IPR018490	; cNMP-bd-like.
IPR018488	; cNMP-bd_CS.
IPR000595	; cNMP-bd_dom.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014710	; RmlC-like_jellyroll.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00027	; cNMP_binding; 2.
PF00069	; Pkinase; 1.

SMARTs

SM00100	; cNMP; 2.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00888	; CNMP_BINDING_1; 2.
PS00889	; CNMP_BINDING_2; 2.
PS50042	; CNMP_BINDING_3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00104

; CGMPKINASE.

Created Date

20-Feb-2013