EKS-DRM-00084

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00084

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	466.3	3.8E-140	196	454	259

Status

Reviewed

Ensembl Protein

FBpp0293606

UniProt Accession

P05130; Q9V7V6; Q9V7V7;

Protein Name

Protein kinase C, brain isozyme

Protein Synonyms/Alias

PKC; dPKC53E(BR);

Gene Name

Pkc53E

Gene Synonyms/Alias

Pkc53E; PKC1; CG6622;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0003091	FBpp0304291	FBtr0331960
FBgn0003091	FBpp0111731	FBtr0112819
FBgn0003091	FBpp0293607	FBtr0305070
FBgn0003091	FBpp0293606	FBtr0305069
FBgn0003091	FBpp0086197	FBtr0087048
FBgn0003091	FBpp0086198	FBtr0087049

Organism

Drosophila melanogaster

Functional Description

PKC is activated by diacylglycerol which in turnphosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Protein Length

525

Protein Sequence
(FASTA)

MNVHARCKEN VPSLCGCDHT ERRGRIYLEI NVKENLLTVQ IKEGRNLIPM DPNGLSDPYV 60

KVKLIPDDKD QSKKKTRTIK ACLNPVWNET LTYDLKPEDK DRRILIEVWD WDRTSRNDFM 120

GALSFGISEI IKNPTNGWFK LLTQDEGEYY NVPCADDEQD LLKLKQKPSQ KKPMVMRSDT 180

NTHTSSKKDM IRATDFNFIK VLGKGSFGKV LLAERKGSEE LYAIKILKKD VIIQDDDVEC 240

TMIEKRVLAL GEKPPFLVQL HSCFQTMDRL FFVMEYVNGG DLMFQIQQFG KFKEPVAVFY 300

AAEIAAGLFF LHTKGILYRD LKLDNVLLDA DGHVKIADFG MCKENIVGDK TTKTFCGTPD 360

YIAPEIILYQ PYGKSVDWWA YGVLLYEMLV GQPPFDGEDE EELFAAITDH NVSYPKSLSK 420

EAKEACKGFL TKQPNKRLGC GSSGEEDVRL HPFFRRIDWE KIENREVQPP FKPKIKHRKD 480

VSNFDKQFTS EKTDLTPTDK VFMMNLDQSE FVGFSYMNPE YVFSP 525

Nucleotide Sequence
(FASTA)

ATGAACGTGC ATGCCCGCTG CAAGGAGAAC GTGCCCAGCC TTTGCGGATG CGATCACACG 60

GAGCGGCGGG GTCGCATTTA TCTGGAGATC AATGTCAAGG AGAATCTGCT AACTGTCCAG 120

ATCAAGGAGG GTCGCAATCT CATACCCATG GACCCAAACG GACTGAGCGA TCCCTATGTG 180

AAGGTGAAGC TAATTCCAGA TGACAAGGAT CAATCCAAGA AGAAGACTCG CACCATTAAG 240

GCTTGCCTAA ATCCTGTCTG GAATGAGACA CTCACTTATG ATCTAAAGCC CGAAGACAAG 300

GATCGACGCA TCCTGATCGA AGTGTGGGAC TGGGATCGCA CTTCACGCAA TGACTTTATG 360

GGTGCTTTGT CCTTCGGAAT CTCAGAGATC ATCAAGAACC CAACCAATGG CTGGTTCAAA 420

CTGCTCACCC AGGACGAAGG CGAGTACTAC AATGTGCCAT GTGCGGATGA TGAGCAGGAC 480

CTACTGAAGC TCAAGCAGAA ACCATCGCAA AAGAAGCCGA TGGTAATGCG CAGCGATACG 540

AACACGCATA CTTCTTCCAA AAAGGATATG ATCCGGGCCA CGGACTTTAA TTTCATCAAA 600

GTTCTCGGCA AGGGCTCATT TGGAAAGGTT TTGCTGGCTG AGCGCAAAGG CAGCGAGGAG 660

TTGTATGCCA TTAAGATACT CAAAAAGGAT GTGATCATTC AGGACGACGA CGTCGAGTGC 720

ACCATGATCG AAAAGCGTGT CCTGGCGCTG GGCGAAAAGC CACCTTTCCT GGTCCAATTA 780

CACTCCTGCT TTCAGACGAT GGACCGTTTG TTCTTTGTAA TGGAGTATGT GAATGGCGGC 840

GATTTGATGT TCCAAATCCA ACAGTTTGGC AAGTTTAAGG AACCAGTGGC CGTATTTTAT 900

GCCGCTGAAA TAGCAGCAGG TCTTTTCTTC CTTCATACCA AGGGCATACT GTATCGAGAT 960

CTAAAGCTGG ATAATGTGCT CCTAGACGCC GATGGACATG TGAAGATTGC GGACTTTGGC 1020

ATGTGCAAGG AGAACATAGT GGGTGATAAG ACCACAAAGA CTTTCTGCGG TACACCTGAT 1080

TACATTGCTC CAGAGATAAT ACTGTATCAG CCCTATGGCA AATCGGTGGA CTGGTGGGCC 1140

TATGGAGTAC TGCTTTATGA GATGTTAGTG GGCCAGCCAC CTTTCGATGG CGAAGATGAG 1200

GAGGAGCTGT TTGCCGCCAT AACTGATCAT AATGTGTCCT ATCCAAAGAG CCTGAGCAAA 1260

GAGGCCAAAG AGGCCTGCAA GGGCTTCCTA ACTAAGCAGC CAAATAAGCG TTTGGGTTGT 1320

GGTTCCTCTG GCGAGGAGGA TGTGCGTCTG CATCCCTTTT TCAGACGCAT CGATTGGGAG 1380

AAAATCGAAA ATCGCGAAGT GCAACCGCCA TTTAAGCCGA AAATTAAACA CCGCAAGGAT 1440

GTGTCCAACT TTGACAAGCA GTTCACATCA GAGAAAACAG ACTTGACGCC CACGGACAAG 1500

GTGTTTATGA TGAATCTGGA TCAGTCGGAA TTCGTTGGCT TCTCCTACAT GAATCCCGAA 1560

TATGTTTTCA GCCCATAG 1578

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp  60

         f+++kvlGkGsfgkvllae+k+++elyaik+lkkdv++qdddve+t++ekrvlal +k+p

Q: 196   FNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPP  255

         99**********************************************************

S: 61    flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg  120

         flvql+scfqt+drlffv+eyvnGGdlmf+iq+ +k+ke+ a+fyaaei+ +l+flh+kg

Q: 256   FLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKG  315

         ************************************************************

S: 121   iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks  180

         i+yrdlkldnvllda+Gh+k+adfG+cke+i+ +ktt+tfcGtPdyiaPei+ +++ygks

Q: 316   ILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKS  375

         ************************************************************

S: 181   vdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkglltkdpe  240

         vdwwa+Gvllyeml+gq+Pf+gede+elf +i++++v+yPkslskea+e +kg+ltk+p+

Q: 376   VDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPN  435

         ************************************************************

S: 241   krlGvkeegeedikehaff  259

         krlG+ ++geed++ h+ff

Q: 436   KRLGCGSSGEEDVRLHPFF  454

         ******************9

Domain Sequence
(FASTA)

FNFIKVLGKG SFGKVLLAER KGSEELYAIK ILKKDVIIQD DDVECTMIEK RVLALGEKPP 60

FLVQLHSCFQ TMDRLFFVME YVNGGDLMFQ IQQFGKFKEP VAVFYAAEIA AGLFFLHTKG 120

ILYRDLKLDN VLLDADGHVK IADFGMCKEN IVGDKTTKTF CGTPDYIAPE IILYQPYGKS 180

VDWWAYGVLL YEMLVGQPPF DGEDEEELFA AITDHNVSYP KSLSKEAKEA CKGFLTKQPN 240

KRLGCGSSGE EDVRLHPFF 259

Keyword

Alternative splicing; ATP-binding; Calcium; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005622	; C:intracellular
GO:0005524	; F:ATP binding
GO:0004697	; F:protein kinase C activity
GO:0008270	; F:zinc ion binding
GO:0035556	; P:intracellular signal transduction
GO:0035206	; P:regulation of hemocyte proliferation

KEGG

dme:Dmel_CG6622

;

InterPros

IPR000961	; AGC-kinase_C.
IPR000008	; C2_Ca-dep.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR020477	; C2_dom.
IPR018029	; C2_membr_targeting.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014375	; Protein_kinase_C_a/b/g.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 2.
PF00168	; C2; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 2.
SM00239	; C2; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 2.

Prints

PR00360	; C2DOMAIN.
PR00008	; DAGPEDOMAIN.

Created Date

20-Feb-2013