EKS-DRM-00073

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00073

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/NDR	499.1	5.0E-150	719	1020	302

Status

Reviewed

Ensembl Protein

FBpp0085082

UniProt Accession

Q9VA38;

Protein Name

Serine/threonine-protein kinase Warts

Protein Synonyms/Alias

Gene Name

wts

Gene Synonyms/Alias

wts; CG12072;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0011739	FBpp0085082	FBtr0085720

Organism

Drosophila melanogaster

Functional Description

Negative regulator of Yorkie (Yki) in the Hippo/SWH(Sav/Wts/Hpo) signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Inhibits nuclear localization of Yki. Regulates salivary gland degradation in a PI3K-dependent manner and Yki- and Sd- independent, mechanism.

Protein Length

1105

Protein Sequence
(FASTA)

MHPAGEKRGG RPNDKYTAEA LESIKQDLTR FEVQNNHRNN QNYTPLRYTA TNGRNDALTP 60

DYHHAKQPME PPPSASPAPD VVIPPPPAIV GQPGAGSISV SGVGVGVVGV ANGRVPKMMT 120

ALMPNKLIRK PSIERDTASS HYLRCSPALD SGAGSSRSDS PHSHHTHQPS SRTVGNPGGN 180

GGFSPSPSGF SEVAPPAPPP RNPTACSAAT PPPPVPPTSQ AYVKRRSPAL NNRPPAIAPP 240

TQRGNSPVIT QNGLKNPQQQ LTQQLKSLNL YPGGGSGAVV EPPPPYLIQG GAGGAAPPPP 300

PPSYTASMQS RQSPTQSQQS DYRKSPSSGI YSATSAGSPS PITVSLPPAP LAKPQPRVYQ 360

ARSQQPIIMQ SVKSTQVQKP VLQTAVAPQS PSSASASNSP VHVLAAPPSY PQKSAAVVQQ 420

QQQAAAAAHQ QQHQHQQSKP PTPTTPPLVG LNSKPNCLEP PSYAKSMQAK AATVVQQQQQ 480

QQQQQQQVQQ QQVQQQQQQQ QQQLQALRVL QAQAQRERDQ RERDQRERER DQQKLANGNP 540

GRQMLPPPPY QSNNNNNSEI KPPSCNNNNI QISNSNLATT PPIPPAKYNN NSSNTGANSS 600

GGSNGSTGTT ASSSTSCKKI KHASPIPERK KISKEKEEER KEFRIRQYSP QAFKFFMEQH 660

IENVIKSYRQ RTYRKNQLEK EMHKVGLPDQ TQIEMRKMLN QKESNYIRLK RAKMDKSMFV 720

KLKPIGVGAF GEVTLVSKID TSNHLYAMKT LRKADVLKRN QVAHVKAERD ILAEADNNWV 780

VKLYYSFQDK DNLYFVMDYI PGGDLMSLLI KLGIFEEELA RFYIAEVTCA VDSVHKMGFI 840

HRDIKPDNIL IDRDGHIKLT DFGLCTGFRW THNSKYYQEN GNHSRQDSME PWEEYSENGP 900

KPTVLERRRM RDHQRVLAHS LVGTPNYIAP EVLERSGYTQ LCDYWSVGVI LYEMLVGQPP 960

FLANSPLETQ QKVINWEKTL HIPPQAELSR EATDLIRRLC ASADKRLGKS VDEVKSHDFF 1020

KGIDFADMRK QKAPYIPEIK HPTDTSNFDP VDPEKLRSND STMSSGDDVD QNDRTFHGFF 1080

EFTFRRFFDD KQPPDMTDDQ APVYV 1105

Nucleotide Sequence
(FASTA)

ATGCATCCAG CGGGCGAAAA AAGGGGCGGT CGCCCCAATG ATAAATACAC GGCGGAAGCC 60

CTCGAGAGCA TCAAGCAGGA CCTAACCCGA TTTGAAGTAC AAAATAACCA TAGGAATAAT 120

CAGAATTACA CACCTCTGCG ATACACGGCG ACCAACGGAC GCAACGATGC ACTTACTCCT 180

GACTATCACC ACGCCAAGCA GCCGATGGAG CCGCCACCCT CCGCCTCTCC TGCTCCGGAC 240

GTGGTCATAC CGCCGCCGCC CGCCATTGTA GGTCAGCCCG GAGCCGGCTC CATATCCGTA 300

TCCGGTGTGG GCGTTGGAGT GGTGGGTGTG GCGAACGGAC GTGTGCCAAA GATGATGACG 360

GCCCTAATGC CAAACAAACT GATCCGGAAG CCGAGCATCG AACGGGACAC GGCGAGCAGT 420

CACTACCTGC GCTGCAGTCC GGCTCTGGAC TCCGGAGCCG GTAGCTCCCG ATCGGACAGC 480

CCCCATTCGC ACCACACCCA CCAGCCGAGC TCGAGGACGG TGGGTAATCC AGGTGGAAAT 540

GGTGGATTTT CTCCGTCGCC AAGCGGTTTC AGTGAGGTGG CTCCACCGGC GCCGCCGCCA 600

CGCAATCCCA CCGCCTGCAG CGCGGCCACG CCCCCACCGC CAGTGCCGCC CACTAGCCAG 660

GCGTACGTGA AGCGGCGATC ACCGGCCCTG AACAACCGCC CGCCGGCGAT AGCGCCACCC 720

ACTCAGCGGG GCAACTCACC TGTAATAACC CAGAACGGGC TGAAGAACCC GCAGCAGCAG 780

TTGACGCAGC AGCTGAAGTC CCTGAACCTA TACCCAGGCG GAGGCAGTGG AGCAGTTGTG 840

GAGCCACCGC CGCCCTACCT AATTCAAGGC GGAGCCGGAG GAGCAGCACC GCCGCCGCCA 900

CCACCCAGTT ACACGGCCTC CATGCAGTCG CGGCAGTCGC CCACACAATC CCAACAATCG 960

GACTACAGGA AATCCCCGAG CAGTGGGATA TACTCGGCCA CCTCGGCGGG CTCGCCGAGC 1020

CCCATAACTG TGTCCCTGCC GCCGGCGCCG CTGGCGAAGC CACAACCACG AGTCTACCAG 1080

GCCAGGAGTC AGCAGCCGAT CATCATGCAG AGTGTGAAGA GCACGCAGGT CCAAAAGCCC 1140

GTGCTGCAAA CAGCAGTGGC GCCCCAATCG CCATCGAGTG CCTCGGCCAG CAATTCACCA 1200

GTCCACGTGC TGGCCGCTCC ACCCTCTTAC CCTCAGAAGT CCGCGGCAGT GGTGCAGCAG 1260

CAGCAACAGG CAGCAGCGGC GGCCCACCAG CAGCAGCATC AGCACCAGCA ATCCAAACCA 1320

CCAACGCCCA CCACACCGCC CTTGGTGGGT CTGAATAGCA AGCCCAATTG CCTGGAGCCA 1380

CCGTCCTATG CCAAGAGCAT GCAGGCCAAG GCGGCCACGG TGGTGCAGCA GCAGCAACAG 1440

CAGCAGCAGC AGCAACAACA GGTCCAGCAG CAGCAGGTGC AACAGCAGCA GCAGCAGCAG 1500

CAACAGCAAC TGCAGGCCTT GAGGGTGCTC CAGGCACAGG CTCAGAGGGA GCGGGATCAA 1560

CGGGAGCGGG ATCAACGGGA GCGGGAACGG GATCAGCAGA AGCTGGCCAA CGGAAATCCT 1620

GGCCGGCAGA TGCTTCCGCC GCCGCCCTAT CAGAGCAACA ACAACAACAA CAGCGAGATC 1680

AAACCGCCGA GCTGCAACAA CAACAACATA CAGATAAGCA ACAGCAACCT GGCGACGACA 1740

CCACCCATTC CGCCTGCCAA ATACAATAAC AACTCCTCCA ACACGGGCGC GAATAGCTCG 1800

GGCGGCAGCA ACGGATCCAC CGGCACCACC GCCTCCTCGT CGACCAGCTG CAAGAAGATC 1860

AAGCACGCCT CGCCCATCCC GGAGCGCAAG AAGATCTCCA AGGAGAAGGA GGAGGAGCGC 1920

AAGGAGTTCC GCATCAGGCA GTACTCGCCG CAAGCCTTCA AGTTCTTCAT GGAGCAGCAC 1980

ATAGAGAACG TGATCAAGTC GTATCGCCAG CGCACGTATC GCAAGAATCA GCTGGAGAAG 2040

GAGATGCACA AAGTGGGACT GCCCGATCAG ACCCAAATCG AGATGAGGAA AATGCTGAAC 2100

CAAAAGGAGA GCAACTACAT TCGATTGAAG CGCGCCAAGA TGGACAAGAG CATGTTCGTC 2160

AAACTGAAGC CCATTGGAGT GGGTGCATTT GGCGAGGTAA CGCTGGTGAG CAAAATCGAT 2220

ACCTCGAACC ATTTGTATGC GATGAAAACC CTGCGGAAAG CGGACGTTCT CAAGCGGAAT 2280

CAGGTGGCAC ACGTGAAGGC CGAGAGGGAT ATCCTCGCGG AAGCCGACAA TAACTGGGTG 2340

GTGAAGTTGT ACTACAGCTT CCAGGACAAG GATAATCTGT ACTTTGTGAT GGACTACATA 2400

CCAGGTGGTG ATCTGATGTC GCTGCTCATC AAACTGGGCA TTTTCGAGGA GGAACTGGCC 2460

AGATTCTACA TCGCCGAGGT CACCTGCGCC GTGGACAGCG TTCACAAAAT GGGCTTCATT 2520

CACAGAGACA TCAAGCCTGA CAACATACTC ATCGATAGGG ACGGACACAT AAAGCTCACC 2580

GACTTTGGCC TGTGCACGGG ATTCCGATGG ACGCACAACT CGAAGTACTA CCAGGAGAAC 2640

GGCAATCACT CGCGCCAGGA CTCGATGGAG CCCTGGGAGG AATACTCCGA GAACGGACCG 2700

AAGCCCACCG TGCTGGAGAG GCGACGGATG CGCGATCACC AAAGAGTCCT GGCCCACTCG 2760

CTGGTGGGCA CCCCGAACTA CATAGCTCCC GAGGTGCTGG AGAGAAGTGG GTACACGCAG 2820

CTGTGCGACT ACTGGAGCGT GGGCGTCATC CTCTATGAGA TGCTGGTGGG TCAGCCGCCC 2880

TTTCTGGCCA ACAGTCCGCT GGAAACGCAA CAAAAGGTCA TCAACTGGGA GAAAACCCTG 2940

CATATTCCGC CGCAGGCCGA GTTATCCCGC GAGGCTACGG ACTTGATAAG GAGGCTCTGT 3000

GCGTCGGCTG ACAAGCGGCT GGGCAAGAGC GTGGACGAGG TCAAGAGCCA CGACTTCTTC 3060

AAGGGCATCG ACTTTGCGGA CATGCGGAAG CAGAAGGCGC CCTATATACC GGAAATCAAG 3120

CACCCGACGG ACACATCCAA CTTTGATCCC GTGGATCCGG AGAAGCTGCG CTCGAATGAC 3180

TCCACCATGA GCAGCGGCGA TGATGTCGAC CAGAACGACC GCACCTTCCA CGGCTTTTTC 3240

GAATTTACCT TCCGTCGCTT CTTCGACGAC AAGCAGCCGC CGGATATGAC GGACGATCAG 3300

GCGCCGGTTT ACGTCTGA 3318

Domain Profile

S: 1     feklkvigkGafGevrlvrkkdt.kevyalkilrkkdllkknqvahvkaerdilaeadse  59

         f klk ig+GafGev+lv+k dt +++ya+k+lrk+d+lk+nqvahvkaerdilaead++

Q: 719   FVKLKPIGVGAFGEVTLVSKIDTsNHLYAMKTLRKADVLKRNQVAHVKAERDILAEADNN  778

         889*******************9779**********************************

S: 60    wvvklyysfqdkenlylvmeylPGGdlmtllikkeilkeelarfyiaelvlavesvhklG  119

         wvvklyysfqdk+nly+vm+y+PGGdlm+llik +i++eelarfyiae+++av+svhk+G

Q: 779   WVVKLYYSFQDKDNLYFVMDYIPGGDLMSLLIKLGIFEEELARFYIAEVTCAVDSVHKMG  838

         ************************************************************

S: 120   fihrdlkPdnllidakGhikltdfGlctGlkkthrseyyk.elekakeddlease.....  173

         fihrd+kPdn+lid++GhikltdfGlctG+++th+s+yy+ + +++++d++e +e

Q: 839   FIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHNSKYYQeNGNHSRQDSMEPWEeysen  898

         ****************************************999**********9999999

S: 174   ..elkvlerrkkkknrrllayslvGtpdyiapevlekkgynklcdwWslGvilyemlvGy  231

           + +vlerr++++++r+la+slvGtp+yiapevle++gy++lcd+Ws+GvilyemlvG+

Q: 899   gpKPTVLERRRMRDHQRVLAHSLVGTPNYIAPEVLERSGYTQLCDYWSVGVILYEMLVGQ  958

         99999*******************************************************

S: 232   pPflaetpqetykkvinwketlkfpeevelseeakdlierliadaekrlgkkGvdeikkh  291

         pPfla++p et++kvinw++tl++p+++els+ea+dli+rl+a+a+krlgk+ vde+k+h

Q: 959   PPFLANSPLETQQKVINWEKTLHIPPQAELSREATDLIRRLCASADKRLGKS-VDEVKSH  1017

         ***************************************************9.*******

S: 292   pff  294

+ff

Q: 1018  DFF  1020

**9

Domain Sequence
(FASTA)

FVKLKPIGVG AFGEVTLVSK IDTSNHLYAM KTLRKADVLK RNQVAHVKAE RDILAEADNN 60

WVVKLYYSFQ DKDNLYFVMD YIPGGDLMSL LIKLGIFEEE LARFYIAEVT CAVDSVHKMG 120

FIHRDIKPDN ILIDRDGHIK LTDFGLCTGF RWTHNSKYYQ ENGNHSRQDS MEPWEEYSEN 180

GPKPTVLERR RMRDHQRVLA HSLVGTPNYI APEVLERSGY TQLCDYWSVG VILYEMLVGQ 240

PPFLANSPLE TQQKVINWEK TLHIPPQAEL SREATDLIRR LCASADKRLG KSVDEVKSHD 300

FF 302

Keyword

ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005815	; C:microtubule organizing center
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007446	; P:imaginal disc growth
GO:0042771	; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:0090090	; P:negative regulation of canonical Wnt receptor signaling pathway
GO:0060253	; P:negative regulation of glial cell proliferation
GO:0046621	; P:negative regulation of organ growth
GO:0045464	; P:R8 cell fate specification
GO:0008360	; P:regulation of cell shape
GO:0048814	; P:regulation of dendrite morphogenesis
GO:0045570	; P:regulation of imaginal disc growth
GO:0010212	; P:response to ionizing radiation
GO:0072089	; P:stem cell proliferation
GO:0060439	; P:trachea morphogenesis

KEGG

dme:Dmel_CG12072

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 2.
PF00433	; Pkinase_C; 1.

SMARTs

SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013