EKS-DRM-00031

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00031

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	334.9	4.8E-100	26	335	310

Status

Reviewed

Ensembl Protein

FBpp0076098

UniProt Accession

Q9VT57; P91642; Q8MS41;

Protein Name

Cyclin-dependent kinase 8

Protein Synonyms/Alias

Cell division protein kinase 8; DmCdk8; Mediator complex subunit Cdk8; Mediator of RNA polymerase II transcription subunit Cdk8;

Gene Name

Cdk8

Gene Synonyms/Alias

Cdk8; CG10572;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0015618	FBpp0076098	FBtr0076369

Organism

Drosophila melanogaster

Functional Description

Component of the Mediator complex, a coactivatorinvolved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation.

Protein Length

454

Protein Sequence
(FASTA)

MDYDFKMKTQ IERTKVEDLF NYEGCKVGRG TYGHVYKAKW KETSDGKEYA LKQIDGTGLS 60

MSACREIALL RELKHQNVIT LIRVFLSHND RKVFLLIDYA EHDLWHIIKF HRAAKATKKQ 120

VVVPRGMVKS LLYQILDGIH YLHSNWVLHR DLKPANILVM GDGNERGRVK IADMGFARLF 180

NAPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 240

IKTSNPYHHD QLDRIFNVMG FPQDKDWEDI KKMPEHHTLT KDFKRSTYST CSLAKYMERH 300

KIKPDSKAFH LLQKLLLMDP NKRITSEQAM QDQYFQEEPQ PTQDVFAGCP IPYPKREFLT 360

DDDQEDKSDN KRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ MNAEPNAKRV RLSGAGNQQD 420

FHHQQQQQQQ QQQQQQQQQQ QMMFNQQQNF QRFN 454

Nucleotide Sequence
(FASTA)

ATGGACTACG ATTTCAAGAT GAAAACGCAG ATAGAGCGTA CGAAAGTGGA GGACCTATTC 60

AACTACGAGG GCTGTAAGGT GGGACGCGGA ACATACGGCC ACGTCTACAA GGCGAAATGG 120

AAGGAGACAA GCGATGGCAA AGAATATGCC TTGAAGCAGA TCGACGGCAC CGGATTGTCC 180

ATGTCCGCCT GCCGCGAGAT CGCATTGCTG CGCGAACTGA AGCATCAGAA CGTAATTACC 240

CTCATCCGGG TGTTTCTGTC GCACAACGAC CGCAAGGTGT TCCTGTTGAT CGACTATGCG 300

GAGCACGACC TGTGGCACAT CATTAAGTTC CATCGGGCTG CTAAGGCCAC CAAAAAGCAG 360

GTTGTGGTTC CCCGCGGTAT GGTTAAAAGT CTGCTATATC AGATTCTGGA TGGGATTCAC 420

TATCTGCACA GTAATTGGGT GCTACATCGT GACCTGAAGC CGGCCAACAT CCTGGTGATG 480

GGCGATGGCA ACGAGAGGGG CCGCGTAAAA ATCGCCGACA TGGGTTTCGC GCGGCTCTTC 540

AATGCCCCAT TGAAACCGCT GGCAGATCTG GATCCCGTGG TGGTTACCTT TTGGTATCGC 600

GCTCCGGAAC TGCTGCTTGG CGCTCGTCAC TACACCAAGG CTATCGACAT CTGGGCTATT 660

GGCTGTATTT TCGCCGAACT ACTCACTTCG GAGCCCATCT TCCATTGCCG CCAGGAGGAC 720

ATAAAGACCA GTAATCCGTA CCATCACGAT CAGCTAGACC GCATCTTCAA TGTGATGGGC 780

TTTCCGCAGG ACAAGGACTG GGAGGATATC AAGAAGATGC CGGAACATCA TACGCTGACA 840

AAGGACTTTA AACGCTCCAC ATACTCCACC TGCTCGCTGG CCAAATACAT GGAAAGGCAC 900

AAAATAAAGC CAGACAGCAA GGCCTTTCAC CTGCTGCAGA AGCTGCTGCT AATGGATCCC 960

AACAAGCGCA TCACCTCCGA ACAGGCCATG CAGGATCAGT ACTTCCAGGA GGAGCCGCAG 1020

CCCACGCAGG ACGTGTTCGC CGGCTGCCCC ATACCGTATC CCAAGCGTGA ATTCCTCACC 1080

GACGACGACC AGGAAGATAA GTCGGATAAT AAGCGACAGC AGCAACAGCA GCAGCAACAA 1140

CAGCAGCAAC AGCAGCAGCA ACAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGCAGCAA 1200

ATGAACGCTG AGCCGAATGC AAAGAGAGTT CGATTGTCCG GAGCTGGTAA CCAGCAAGAT 1260

TTTCACCACC AGCAGCAGCA GCAACAGCAG CAGCAGCAAC AACAGCAGCA GCAGCAGCAA 1320

CAGATGATGT TCAATCAGCA GCAGAACTTC CAGCGCTTCA ACTGA 1365

Domain Profile

S: 6     kigeGtygkvykakdke..tgevvAlKkirlekekegvpitalrEisllkelkhknivkL  63

         k+g+Gtyg+vykak ke  +g+ +AlK+i    + +g++++a+rEi+ll+elkh+n+++L

Q: 26    KVGRGTYGHVYKAKWKEtsDGKEYALKQI----DGTGLSMSACREIALLRELKHQNVITL  81

         79***********9977225679******....899************************

S: 64    levvakdk.kklyLvfefleqdLkkllkkkkkk.......klsleevkslllqlleglay  115

         + v+ +++ +k++L+++++e+dL++++k ++++        +++ +vksll+q+l+g++y

Q: 82    IRVFLSHNdRKVFLLIDYAEHDLWHIIKFHRAAkatkkqvVVPRGMVKSLLYQILDGIHY  141

         ****95555********************9998889999899999***************

S: 116   lHsnkilHRDlKpqNlLiske....gklklaDfGlarafssplk...tytkevvTlwYra  168

         lHsn++lHRDlKp+N+L++ +    g++k+aD+G+ar f++plk   +++++vvT+wYra

Q: 142   LHSNWVLHRDLKPANILVMGDgnerGRVKIADMGFARLFNAPLKplaDLDPVVVTFWYRA  201

         ******************98877779*************99999665699**********

S: 169   PelLlgakeYstavDiWsvgcilaelltrkplfqgkse.........idqlerifkllgt  219

         PelLlga++Y++a+DiW++gci+aellt++p+f++++e         +dql+rif+++g

Q: 202   PELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEdiktsnpyhHDQLDRIFNVMGF  261

         ************************************************************

S: 220   psekvwpevsklpeyk...ksfpkkkkk..klkekv..kklk..ekaldllekllaldpk  270

         p++k+w++++k+pe++   k+f++++++  +l++++  +k+k  +ka++ll+kll +dp+

Q: 262   PQDKDWEDIKKMPEHHtltKDFKRSTYStcSLAKYMerHKIKpdSKAFHLLQKLLLMDPN  321

         **************996655787777765577777744444465679*************

S: 271   kRisakealqhkyf  284

         kRi++++a+q++yf

Q: 322   KRITSEQAMQDQYF  335

         *************9

Domain Sequence
(FASTA)

KVGRGTYGHV YKAKWKETSD GKEYALKQID GTGLSMSACR EIALLRELKH QNVITLIRVF 60

LSHNDRKVFL LIDYAEHDLW HIIKFHRAAK ATKKQVVVPR GMVKSLLYQI LDGIHYLHSN 120

WVLHRDLKPA NILVMGDGNE RGRVKIADMG FARLFNAPLK PLADLDPVVV TFWYRAPELL 180

LGARHYTKAI DIWAIGCIFA ELLTSEPIFH CRQEDIKTSN PYHHDQLDRI FNVMGFPQDK 240

DWEDIKKMPE HHTLTKDFKR STYSTCSLAK YMERHKIKPD SKAFHLLQKL LLMDPNKRIT 300

SEQAMQDQYF 310

Keyword

Activator; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0016592	; C:mediator complex
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0001104	; F:RNA polymerase II transcription cofactor activity
GO:0007411	; P:axon guidance
GO:0022416	; P:chaeta development
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0036011	; P:imaginal disc-derived leg segmentation
GO:0007095	; P:mitotic cell cycle G2/M transition DNA damage checkpoint
GO:0045498	; P:sex comb development
GO:0006367	; P:transcription initiation from RNA polymerase II promoter

KEGG

dme:Dmel_CG10572

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013