EKS-DRM-00027

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00027

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	363.7	7.8E-109	384	677	294

Status

Reviewed

Ensembl Protein

FBpp0293474

UniProt Accession

Q9VPC0; Q5U100; Q8MQR7; Q94889; Q9TXB3;

Protein Name

Serine/threonine-protein kinase PITSLRE

Protein Synonyms/Alias

Cell division cycle 2-like;

Gene Name

Pitslre

Gene Synonyms/Alias

Pitslre; CG4268;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0016696	FBpp0293474	FBtr0304935
FBgn0016696	FBpp0293475	FBtr0304936
FBgn0016696	FBpp0077905	FBtr0078247
FBgn0016696	FBpp0077908	FBtr0078250
FBgn0016696	FBpp0303913	FBtr0331523

Organism

Drosophila melanogaster

Functional Description

Acts as a negative regulator of the normal cell cycleprogression. May function in regulating proliferation by the phosphorylation and subsequent plasma membrane targeting of galactosyltransferase (By similarity).

Protein Length

778

Protein Sequence
(FASTA)

MEEPTRRSSK YAESKDAESL EQDLRSRLLK KRHNYVKDYE TEENYEHRVE RSDRREGGRK 60

ERERTVRSTH KQNRHDRVIE LLDSPEQEHH HQHQHKSHRS KWREEVEVIR RKVPEDLELL 120

ARREKLLAAE RESRQRKQTA REELEARREL LRERNEHSDA LSPTTVAASV TAGLNIHVKR 180

KSKPDNYEKE IKLKKRREDD IEVIRDDDDE ESEESDSNEE VPEQDSEGSA TESGSEDSYA 240

SKKKSKIKSK SQLEDDDEDL PLPDSPLSVG ELYKSPKQRQ RSRSVSSKSS SQSSRSSRSR 300

SRSRSQSSLE DEVDRQDVGA DASPSSSTRS EERGMTQEQP EEKPEEKLKE KQKSLEEQIP 360

CDDKGIPLPN YYPGVQGCRS VEEFQCLNRI EEGTYGVVYR AKDKRTNEIV ALKRLKMEKE 420

KEGFPITSLR EINTLLKGQH PNIVTVREIV VGSNMDKIFI VMDYVEHDLK SLMETMKNRK 480

QSFFPGEVKC LTQQLLRAVA HLHDNWILHR DLKTSNLLLS HKGILKVGDF GLAREYGSPI 540

KKYTSLVVTL WYRAPELLLC SPVYSTPIDV WSVGCIFAEF LQMLPLFPGK SEIDELNRIF 600

KELGTPNEKI WPGYTELPAV KNMLSQNSQF TEYPVSQLRK HFQEKTSEMG LSLLQGLLTY 660

DPKQRLSADA ALKHGFFKEL PLPIDPSMFP TWPAKSELGA RKAQASSPKP PSGGSQFKQL 720

GRDEPIIVGP GNKLSSGIIT GNKKSHGAGG SSASTGFVLN AGITQRQLAM GPGFSLKF 778

Nucleotide Sequence
(FASTA)

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 60

GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 120

ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 180

GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 240

CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 300

AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 360

GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 420

CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 480

CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 540

AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 600

ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 660

GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 720

AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 780

CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 840

CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 900

TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 960

GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 1020

GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 1080

TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 1140

GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 1200

GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 1260

AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 1320

CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 1380

GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 1440

CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 1500

CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 1560

CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 1620

AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 1680

TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 1740

TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 1800

AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 1860

AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 1920

CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 1980

GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 2040

CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 2100

CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 2160

GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 2220

GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 2280

GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA 2337

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekekegvpitalrEisllkelkhkni  60

         +++l++i+eGtyg+vy+akdk+t+e+vAlK++++ekekeg+pit+lrEi++l + +h+ni

Q: 384   FQCLNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNI  443

         7899********************************************************

S: 61    vkLlevva.kdkkklyLvfefleqdLkkllkkkkkk..klsleevkslllqlleglaylH  117

         v++ e+v+ ++ +k+++v++++e+dLk+l++++k++  ++ + evk l++qll+++a+lH

Q: 444   VTVREIVVgSNMDKIFIVMDYVEHDLKSLMETMKNRkqSFFPGEVKCLTQQLLRAVAHLH  503

         *******95666*******************99988778999******************

S: 118   snkilHRDlKpqNlLiskegklklaDfGlarafssplktytkevvTlwYraPelLlgake  177

         +n+ilHRDlK++NlL+s++g+lk++DfGlar+++sp k+yt+ vvTlwYraPelLl +

Q: 504   DNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSLVVTLWYRAPELLLCSPV  563

         ************************************************************

S: 178   YstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsklpeyk.k  236

         Yst +D+Wsvgci+ae+l+  plf+gkseid+l+rifk lgtp+ek+wp++++lp +k +

Q: 564   YSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKnM  623

         *********************************************************963

S: 237   .....sfpkkkkkklkekvkklkek.aldllekllaldpkkRisakealqhkyf  284

              +f++++ ++l++++++ +++ +l+ll+ ll++dpk+R+sa++al+h +f

Q: 624   lsqnsQFTEYPVSQLRKHFQEKTSEmGLSLLQGLLTYDPKQRLSADAALKHGFF  677

         6777789999999******7766555*************************998

Domain Sequence
(FASTA)

FQCLNRIEEG TYGVVYRAKD KRTNEIVALK RLKMEKEKEG FPITSLREIN TLLKGQHPNI 60

VTVREIVVGS NMDKIFIVMD YVEHDLKSLM ETMKNRKQSF FPGEVKCLTQ QLLRAVAHLH 120

DNWILHRDLK TSNLLLSHKG ILKVGDFGLA REYGSPIKKY TSLVVTLWYR APELLLCSPV 180

YSTPIDVWSV GCIFAEFLQM LPLFPGKSEI DELNRIFKEL GTPNEKIWPG YTELPAVKNM 240

LSQNSQFTEY PVSQLRKHFQ EKTSEMGLSL LQGLLTYDPK QRLSADAALK HGFF 294

Keyword

ATP-binding; Cell cycle; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007155	; P:cell adhesion
GO:0007049	; P:cell cycle
GO:0008360	; P:regulation of cell shape

KEGG

dme:Dmel_CG4268

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013