EKS-DRM-00178

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00178

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	315.4	3.6E-94	20	264	245

Status

Reviewed

Ensembl Protein

FBpp0077011

UniProt Accession

Q9V3Q6;

Protein Name

Mitogen-activated protein kinase kinase kinase 7

Protein Synonyms/Alias

TGF-beta-activated kinase 1; dTAK1;

Gene Name

Tak1

Gene Synonyms/Alias

Tak1; CG18492;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0026323	FBpp0077011	FBtr0077319

Organism

Drosophila melanogaster

Functional Description

Component of a protein kinase signal transductioncascade. Mediator of TGF-beta signal transduction. Responsible for activation of the JNK MAPK pathway (basket, bsk and hemipterous, hep) in response to LPS. Component of the NF-kappa-B pathway; relish-mediated JNK inhibition involves proteasomal degradation of Tak1; certain targets of Relish that are induced during immune responses may facilitate destruction of Tak1 and switch off the JNK cascade. Participates in diverse roles such as control of cell shape and regulation of apoptosis.

Protein Length

678

Protein Sequence
(FASTA)

MATASLDALQ AAYVDFSEIT LREKVGHGSY GVVCKAVWRD KLVAVKEFFA SAEQKDIEKE 60

VKQLSRVKHP NIIALHGISS YQQATYLIME FAEGGSLHNF LHGKVKPAYS LAHAMSWARQ 120

CAEGLAYLHA MTPKPLIHRD VKPLNLLLTN KGRNLKICDF GTVADKSTMM TNNRGSAAWM 180

APEVFEGSKY TEKCDIFSWA IVLWEVLSRK QPFKGIDNAY TIQWKIYKGE RPPLLTTCPK 240

RIEDLMTACW KTVPEDRPSM QYIVGVMHEI VKDYTGADKA LEYTFVNQQI VTKESDGTVA 300

AQPDSLSSQE GELSPSSTQL TPTTAANANV NAIAISKTTT SSMTENTSST SSDITPTNSG 360

QLDNNPLFYM VTNRWDAIPE EESNESRNDS FNLTSSAEAT QRLETIRNGM ILMACKPMEQ 420

LTLDVEANGF DLSPSESSSS STNAKSDGRE RLTVTDTKPV MMTTDLSNNN GGIHAHSNGL 480

LSHANGWQAR DEELQEQEHE QEIVNSLDVD VDPDEDENDG TEQSLAEILD PELQPEPPIP 540

NDAESQLIYR DHRHMAKEYL SVDTNLYYAQ DFKDKLIVQM DRTEREQKQE LLRKMKDKEG 600

LQSLYNNLQQ QYASRQLAAG HHPQPHPHPH PNQLQHPHSH PPMHFLQDEG CGLLPGSVCG 660

GSESVEEGWV VIPPHHNA 678

Nucleotide Sequence
(FASTA)

ATGGCCACAG CATCGCTGGA CGCACTGCAG GCAGCCTATG TGGACTTCAG TGAGATAACA 60

CTAAGAGAGA AAGTCGGCCA TGGGTCCTAC GGAGTGGTCT GCAAGGCCGT TTGGCGCGAC 120

AAGCTGGTTG CCGTCAAGGA GTTCTTCGCC AGCGCCGAGC AGAAGGACAT CGAGAAGGAG 180

GTGAAGCAGT TGTCGCGCGT GAAGCACCCG AACATCATCG CTCTGCACGG GATATCCTCG 240

TACCAGCAGG CCACCTACCT GATAATGGAG TTCGCCGAAG GTGGATCGCT GCACAACTTC 300

CTTCACGGCA AGGTGAAGCC GGCATATTCT CTGGCCCACG CCATGAGCTG GGCGCGCCAA 360

TGTGCAGAGG GTCTGGCATA TTTGCATGCC ATGACGCCAA AACCACTAAT ACATCGCGAC 420

GTGAAGCCGC TGAACCTGCT CTTGACCAAC AAGGGACGCA ATCTGAAGAT ATGCGACTTC 480

GGCACGGTGG CGGACAAGTC GACCATGATG ACCAACAATC GCGGCAGTGC CGCTTGGATG 540

GCGCCCGAGG TCTTCGAAGG CTCCAAGTAT ACGGAGAAGT GTGACATTTT TAGCTGGGCC 600

ATTGTTCTAT GGGAGGTTCT GTCCAGGAAG CAGCCCTTTA AAGGCATCGA CAATGCCTAC 660

ACCATCCAGT GGAAGATCTA CAAGGGTGAA CGCCCGCCGC TGCTGACCAC TTGCCCCAAG 720

CGCATCGAGG ACCTGATGAC CGCCTGCTGG AAAACGGTGC CCGAGGATCG CCCGTCGATG 780

CAGTACATAG TGGGCGTTAT GCACGAGATC GTCAAGGACT ATACGGGGGC GGACAAGGCC 840

CTGGAATACA CGTTTGTTAA TCAACAGATT GTCACCAAAG AGAGCGACGG CACGGTGGCC 900

GCTCAACCGG ATAGCCTCAG TTCGCAGGAG GGGGAACTGA GCCCCTCGTC CACACAGTTA 960

ACACCGACAA CGGCGGCCAA CGCCAATGTG AACGCGATAG CAATATCAAA AACAACGACT 1020

AGCTCAATGA CCGAAAATAC CTCATCAACA TCATCGGACA TCACGCCGAC GAACTCGGGC 1080

CAACTGGACA ATAATCCGCT ATTCTATATG GTCACCAATC GCTGGGACGC GATTCCCGAG 1140

GAGGAGAGCA ACGAGAGCCG GAACGATAGC TTCAACCTCA CCTCGTCGGC TGAGGCCACT 1200

CAGCGCCTCG AAACGATCCG GAACGGCATG ATCCTGATGG CCTGCAAGCC CATGGAGCAG 1260

CTCACCCTCG ACGTGGAGGC GAATGGCTTT GATCTGAGTC CCAGCGAAAG CAGCAGCAGC 1320

AGCACGAACG CAAAGAGCGA TGGCCGCGAA CGACTCACGG TGACGGACAC CAAGCCGGTG 1380

ATGATGACCA CGGATCTGTC CAACAACAAC GGCGGCATCC ACGCCCACTC GAACGGACTG 1440

CTGAGCCATG CGAATGGTTG GCAAGCAAGA GATGAGGAGC TGCAGGAGCA AGAGCATGAG 1500

CAGGAGATTG TCAACTCGTT GGACGTCGAC GTGGATCCCG ACGAGGATGA GAACGACGGC 1560

ACCGAACAGT CACTGGCCGA GATTCTTGAT CCGGAGCTCC AGCCAGAGCC GCCGATACCC 1620

AACGATGCCG AATCGCAGCT CATCTACCGG GACCACCGAC ACATGGCCAA GGAGTACCTG 1680

AGCGTCGACA CGAACCTCTA CTACGCGCAG GACTTCAAGG ACAAGCTCAT CGTGCAGATG 1740

GACCGAACCG AGCGCGAACA GAAGCAGGAG CTTCTGCGCA AGATGAAGGA CAAGGAGGGT 1800

CTTCAGAGTC TTTACAACAA TCTGCAGCAG CAGTACGCTT CACGCCAACT TGCGGCCGGC 1860

CATCATCCGC AACCTCATCC TCATCCGCAT CCAAATCAGC TTCAGCATCC ACACTCACAT 1920

CCGCCTATGC ATTTCCTGCA GGATGAGGGC TGTGGACTGC TGCCCGGATC GGTGTGCGGA 1980

GGCTCTGAGT CCGTGGAAGA AGGCTGGGTG GTCATCCCAC CGCATCACAA TGCGTAG 2037

Domain Profile

S: 2     eleeligsgafgkvykakwrkkevavKklreeaeekekleklereakllsklshpniiel  61

         +l+e++g+g++g v+ka wr+k vavK++ ++ae+    +++e+e+k+ls+++hpnii+l

Q: 20    TLREKVGHGSYGVVCKAVWRDKLVAVKEFFASAEQ----KDIEKEVKQLSRVKHPNIIAL  75

         6789***************************9999....*********************

S: 62    lGvcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseapkk  121

         +G+++ +++++l+me++eggsL+n+lh+k k++++l++++swa q+A+G+aYLh ++pk+

Q: 76    HGISSYQQATYLIMEFAEGGSLHNFLHGKVKPAYSLAHAMSWARQCAEGLAYLHAMTPKP  135

         ************************************************************

S: 122   iihrdLksknvLlseekktlkicdFGtarelskstkmsaagtlawmaPEvlkeekvseks  181

         +ihrd+k+ n+Ll+++ ++lkicdFGt+ + s + +++++g++awmaPEv++ +k++ek+

Q: 136   LIHRDVKPLNLLLTNKGRNLKICDFGTVADKS-TMMTNNRGSAAWMAPEVFEGSKYTEKC  194

         ******************************77.8999999********************

S: 182   DvwSygvvLWelltrevPykdlse.laiaykvalkglrlpipsscpeklkkLmkecwned  240

         D++S+++vLWe+l+r++P+k++++ ++i++k+ +kg+r+p+ ++cp+++++Lm+ cw++

Q: 195   DIFSWAIVLWEVLSRKQPFKGIDNaYTIQWKI-YKGERPPLLTTCPKRIEDLMTACWKTV  253

         ***********************967999**9.9**************************

S: 241   pekRpsfseiv  251

         pe+Rps++ iv

Q: 254   PEDRPSMQYIV  264

         ********998

Domain Sequence
(FASTA)

TLREKVGHGS YGVVCKAVWR DKLVAVKEFF ASAEQKDIEK EVKQLSRVKH PNIIALHGIS 60

SYQQATYLIM EFAEGGSLHN FLHGKVKPAY SLAHAMSWAR QCAEGLAYLH AMTPKPLIHR 120

DVKPLNLLLT NKGRNLKICD FGTVADKSTM MTNNRGSAAW MAPEVFEGSK YTEKCDIFSW 180

AIVLWEVLSR KQPFKGIDNA YTIQWKIYKG ERPPLLTTCP KRIEDLMTAC WKTVPEDRPS 240

MQYIV 245

Keyword

ATP-binding; Complete proteome; Immunity; Innate immunity; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004706	; F:JUN kinase kinase kinase activity
GO:0046872	; F:metal ion binding
GO:0006915	; P:apoptotic process
GO:0071222	; P:cellular response to lipopolysaccharide
GO:0050829	; P:defense response to Gram-negative bacterium
GO:0051607	; P:defense response to virus
GO:0007391	; P:dorsal closure
GO:0007249	; P:I-kappaB kinase/NF-kappaB cascade
GO:0006955	; P:immune response
GO:0045087	; P:innate immune response
GO:0048802	; P:notum morphogenesis
GO:0016318	; P:ommatidial rotation
GO:0006963	; P:positive regulation of antibacterial peptide biosynthetic process
GO:0046330	; P:positive regulation of JNK cascade

KEGG

dme:Dmel_CG18492

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF07714

; Pkinase_Tyr; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013