EKS-DRM-00135

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00135

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	371.2	3.6E-111	14	267	254

Status

Reviewed

Ensembl Protein

FBpp0078042

UniProt Accession

O97143; Q86NL8;

Protein Name

Serine/threonine-protein kinase PLK4

Protein Synonyms/Alias

Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase SAK;

Gene Name

SAK

Gene Synonyms/Alias

SAK; CG7186;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0026371	FBpp0078042	FBtr0078387

Organism

Drosophila melanogaster

Functional Description

Serine/threonine-protein kinase that plays a centralrole in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.

Protein Length

769

Protein Sequence
(FASTA)

MLSNRAFGET IEDYEVQHLL GKGGFATVYK ARCLHTHQDV AIKMIDKKLI QGTGLTNRVR 60

QEVEIHSRLK HPSVLQLYTF FQDANYVYLV LELAHNGELH RYMNHIARPF TETEAASILK 120

QVVAGLLYLH SHNIMHRDIS LSNLLLSREM HVKIADFGLA TQLKRPDERH MTMCGTPNYI 180

SPEVVSRTSH GLPADVWSVG CMLYTLLVGR PPFETDAVQS TLNKVVMSEY IMPAHLSYEA 240

QDLINKLLKK LPHERITLEA VLCHPFMLKC SNGGHSAPGA LNVFSQSMES GDSGIITFAS 300

SDSRNSQQIR SVENSGPQQV LPQIREEFKQ VHHKLPYEQT GLFGQASTGL AEPNWPGAAK 360

SSAFCMEAGN VPNSKQASLK EDRISVPPLN TKRLLPTRYK TKNAIMSILR NGEVVLEFLK 420

FRPTYNEDRI NDICRISDDG QRIIIYQPDP GRGLPVREQP PDLQIPSGDC VYNYDNLPSK 480

HWKKYIYGAR FVGLVKSKTP KVTYFSTLGK CQLMETMTDF EIRFYSGAKL LKTPSEGLKV 540

YDRNGMLLSD YSCSESRSLI EHGNECFTHC VNISNALEVA QTKDNSCFPV TIGRRPITDV 600

QPAQRLDGLR DTTNIAFSTP KSNQGSINFS LSTISSTRNT SDFGTNCSRS NMLAAHQNIP 660

IKRINVPEIG IATELSHGVV QVQFYDGSVV SVIPSMQGGG ITYTQPNGTS THFGKGDDLP 720

FPVRDRVGQI PNIQLKLKTA PLLGSGRKTD YNNAMTPKTT TPYYNRMLL 769

Nucleotide Sequence
(FASTA)

ATGTTATCCA ATCGGGCGTT TGGAGAAACA ATTGAGGACT ATGAAGTACA GCACTTGCTG 60

GGTAAAGGTG GTTTTGCGAC CGTCTACAAG GCGCGGTGCC TGCACACTCA CCAGGATGTG 120

GCCATAAAGA TGATCGATAA AAAACTAATC CAGGGCACTG GACTCACTAA CCGCGTTCGC 180

CAGGAAGTGG AAATCCACTC CCGCCTGAAG CATCCCTCTG TGCTTCAGCT GTACACTTTC 240

TTTCAGGACG CCAACTATGT GTATTTGGTG CTCGAGCTGG CCCATAATGG GGAGCTTCAT 300

CGCTATATGA ACCACATCGC CAGACCCTTC ACAGAGACGG AAGCTGCTTC CATTCTGAAG 360

CAGGTGGTGG CGGGACTTTT GTACCTGCAC TCCCACAACA TCATGCACCG CGACATTTCG 420

CTGTCCAACC TACTGCTTAG CAGGGAAATG CACGTCAAGA TAGCCGATTT TGGACTGGCC 480

ACCCAATTAA AGCGACCTGA TGAGCGCCAT ATGACCATGT GTGGAACTCC GAACTATATT 540

TCGCCTGAGG TGGTATCGCG AACGTCTCAC GGACTGCCCG CGGACGTCTG GAGCGTTGGA 600

TGCATGCTGT ACACCCTGCT GGTTGGACGC CCGCCCTTCG AAACCGATGC AGTGCAGTCA 660

ACCCTTAACA AAGTTGTGAT GTCCGAGTAC ATTATGCCGG CTCACTTGTC TTACGAGGCG 720

CAGGACCTGA TAAACAAATT ATTAAAAAAG CTCCCGCACG AACGAATTAC CCTGGAGGCT 780

GTTCTCTGCC ATCCCTTTAT GTTGAAATGC TCAAATGGTG GACACTCTGC GCCGGGAGCG 840

TTGAATGTGT TCAGCCAGAG TATGGAAAGT GGCGATAGTG GAATCATAAC ATTTGCTAGC 900

AGTGACTCCC GGAATTCCCA GCAAATTCGA TCTGTGGAAA ACTCAGGACC ACAACAAGTG 960

CTTCCTCAAA TACGGGAGGA ATTTAAGCAA GTCCATCATA AATTGCCTTA TGAACAGACA 1020

GGTTTATTTG GGCAAGCTTC AACTGGCTTG GCAGAGCCAA ATTGGCCAGG AGCTGCTAAG 1080

TCCTCAGCGT TCTGCATGGA GGCGGGCAAT GTACCCAATT CCAAACAGGC ATCTCTTAAG 1140

GAAGATCGCA TTTCGGTGCC ACCATTGAAT ACTAAAAGAC TGCTTCCGAC GCGTTATAAG 1200

ACGAAGAATG CTATAATGAG TATTTTGCGC AATGGAGAGG TAGTTCTCGA GTTTCTTAAG 1260

TTTCGGCCAA CCTATAATGA AGATCGCATT AATGATATTT GTCGCATATC GGATGACGGA 1320

CAGCGAATCA TAATTTATCA ACCAGATCCT GGGCGTGGCT TACCAGTGAG AGAGCAGCCA 1380

CCAGATCTTC AAATACCAAG CGGAGATTGT GTATATAACT ACGATAATTT GCCCAGCAAG 1440

CACTGGAAAA AATACATATA CGGAGCTCGA TTTGTAGGGC TGGTAAAAAG CAAAACACCA 1500

AAAGTCACAT ACTTCAGTAC TCTAGGCAAA TGCCAATTGA TGGAGACAAT GACCGACTTT 1560

GAGATTCGTT TCTACTCGGG AGCTAAGCTA CTAAAGACGC CGTCCGAGGG GCTAAAGGTC 1620

TACGATCGAA ATGGAATGTT GCTCTCGGAT TATTCCTGTT CGGAGTCACG ATCCCTAATA 1680

GAGCACGGAA ACGAGTGTTT CACCCACTGT GTCAACATCA GTAATGCCTT GGAAGTAGCT 1740

CAGACGAAAG ATAATTCGTG TTTTCCAGTC ACAATCGGAC GAAGACCTAT AACAGATGTA 1800

CAACCCGCTC AGAGATTGGA TGGCCTACGA GATACCACCA ATATTGCGTT TTCCACACCA 1860

AAATCTAATC AGGGCTCAAT AAACTTTTCC TTAAGCACTA TTTCGTCGAC TCGAAATACT 1920

TCAGATTTTG GAACCAACTG CTCTAGGTCC AACATGCTAG CCGCCCATCA AAATATACCC 1980

ATCAAACGCA TAAACGTTCC TGAAATCGGA ATAGCTACTG AGCTCTCCCA TGGAGTTGTC 2040

CAAGTGCAAT TTTATGATGG ATCCGTGGTC TCTGTTATTC CGAGCATGCA AGGTGGTGGT 2100

ATAACCTATA CCCAACCCAA CGGAACTTCA ACCCACTTCG GCAAGGGCGA CGACTTGCCA 2160

TTCCCGGTCA GAGATAGAGT CGGTCAGATA CCAAACATTC AGTTAAAGTT GAAGACAGCT 2220

CCATTGCTGG GAAGCGGCAG GAAAACTGAC TACAACAATG CAATGACTCC CAAAACGACA 2280

ACGCCTTATT ATAATCGCAT GCTTCTTTAA 2310

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         ye+++llGkGgfa++y++++l+t++ +a+k+++k+l++ + ++++v++e+eih++lkh++

Q: 14    YEVQHLLGKGGFATVYKARCLHTHQDVAIKMIDKKLIQGTGLTNRVRQEVEIHSRLKHPS  73

         899*********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrr.kaltepevryylrqivsglkylhekr  119

         +++ly+ff+d+++vylvlel+++++l++++++  +++te+e++ +l+q+v+gl+ylh+++

Q: 74    VLQLYTFFQDANYVYLVLELAHNGELHRYMNHIaRPFTETEAASILKQVVAGLLYLHSHN  133

         *******************************998**************************

S: 120   ilhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghsle  179

         i+hrd++l+nl+l++e++vki+dfGlat+l++++er++t+cGtPnyi+Pev+s+++h+l

Q: 134   IMHRDISLSNLLLSREMHVKIADFGLATQLKRPDERHMTMCGTPNYISPEVVSRTSHGLP  193

         ************************************************************

S: 180   vdiWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePe  239

         +d+Ws+Gc+lytllvG+PPfet+++++t++k++++ey++P++ls ea++li+kllkk P+

Q: 194   ADVWSVGCMLYTLLVGRPPFETDAVQSTLNKVVMSEYIMPAHLSYEAQDLINKLLKKLPH  253

         ************************************************************

S: 240   erpsleevlkdefl  253

         er++le+vl+++f+

Q: 254   ERITLEAVLCHPFM  267

         ************98

Domain Sequence
(FASTA)

YEVQHLLGKG GFATVYKARC LHTHQDVAIK MIDKKLIQGT GLTNRVRQEV EIHSRLKHPS 60

VLQLYTFFQD ANYVYLVLEL AHNGELHRYM NHIARPFTET EAASILKQVV AGLLYLHSHN 120

IMHRDISLSN LLLSREMHVK IADFGLATQL KRPDERHMTM CGTPNYISPE VVSRTSHGLP 180

ADVWSVGCML YTLLVGRPPF ETDAVQSTLN KVVMSEYIMP AHLSYEAQDL INKLLKKLPH 240

ERITLEAVLC HPFM 254

Keyword

3D-structure; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007099	; P:centriole replication
GO:0007052	; P:mitotic spindle organization
GO:0046599	; P:regulation of centriole replication
GO:0007288	; P:sperm axoneme assembly

KEGG

dme:Dmel_CG7186

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008266	; Tyr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013